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Citations for Peprotech :
151 - 189 of 189 citations for Cow Melanocyte Stimulating Hormone Receptor MC1R ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... 55 uM 2-mercaptoethanol and 20 ng/ml of recombinant murine granulocyte-macrophage colony-stimulating factor (PeproTech, Rocky Hill, NJ, United States)] at 1:5 apoptotic cancer cell to cDC1 ratio ...
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bioRxiv - Microbiology 2023Quote: ... and 50 μM β-mercaptoethanol and differentiated with 20 ng/ml murine granulocyte-macrophage colony-stimulating factor (GM-CSF) (Peprotech, 315-03). BMDCs were stained for CD11c and analyzed by flow cytometry (LSR-Fortessa flow cytometer ...
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bioRxiv - Immunology 2023Quote: ... in the presence of 40 ng/ml of granulocyte-macrophage colony-stimulating factor (GM-CSF) and IL-4 (both from Peprotech or Biolegend). Cells were cultured in 24-well plates in DMEM (Gibco) ...
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bioRxiv - Immunology 2024Quote: ... cells were isolated from bone marrow of C57Bl/6 mice and cultured with granulocyte-macrophage stimulating factor (100ng or 2×103 units per 3×106 cells, Peprotech, Cranbury, NJ) in RPMI-1640 media containing 10% heat inactivated fetal bovine serum ...
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bioRxiv - Cell Biology 2023Quote: ... BMMs were seeded at a density of 1.2 × 105 cells/well into 24-well culture plates and incubated in α-MEM (SH30265.01; Hyclone, Rockford, IL, USA) containing 20 ng/mL macrophage colony-stimulating factor (M-CSF) (300-25; PeproTech, Cranbury, NJ, USA). To induce osteoclast differentiation ...
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bioRxiv - Cell Biology 2023Quote: ... and 50 ng/ml receptor activator of nuclear factor κB ligand (RANKL, 310-01, PeproTech). Explants were cultured for 20 days in an incubator at 37°C with 5% CO2 and medium was replaced every 2 days in both compartments ...
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bioRxiv - Biochemistry 2023Quote: ... The expression of IL-6 in culture supernatants was detected using the Murine IL-6 Mini ABTS ELISA Development Kit (PeproTech, 900-M50) according to the manufacturer’s protocol.
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bioRxiv - Immunology 2023Quote: ... Supernatants were harvested and used in triplicate to measure the concentration of VEGF-A by ELISA using the kit from PeproTech (Cranbury, NJ). In another experiment ...
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bioRxiv - Immunology 2020Quote: ... Isolated PBMC were washed with PBS and resuspended by RPMI-1640 which supplemented 50ng/ml recombinant human macrophage colony-stimulating factor (M-CSF) (AF-300-25-500, PeproTech, Cranbury, NJ, USA), then cultured at 37°C in 5% CO2 for 5 days to differentiate into human monocyte derived macrophages (hMDMs ...
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bioRxiv - Neuroscience 2022Quote: ... 10k primary rat microglia were added to 20k DIV12 primary hippocampal neurons with 40 ng/mL of rat macrophage colony stimulating factor (MCSF) (Peprotech 400-28-100UG). At the time of plating ...
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bioRxiv - Microbiology 2023Quote: ... 100 ⍰g/ml streptomycin and 50 ⍰M β-mercaptoethanol plus 20 ng/ml murine granulocyte-macrophage colony-stimulating factor (GM-CSF) (Peprotech, 315-03). On day 4 ...
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bioRxiv - Bioengineering 2022Quote: ... The supernatant was discarded and the cells were re-suspended in BMDM (the same supplemented DMEM used for nerve fibroblasts, further supplemented with macrophage colony stimulating factor [400-28, Peprotech; 50 ng/ml]). Cells were counted in a hemocytometer and seeded on 100 x 15 mm uncoated petri dishes (Sigma ...
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bioRxiv - Immunology 2024Quote: ... and cultured in DC media (15% FBS, 1% A/A, 50µM α-MTG, 20ng/mL granulocyte-macrophage colony stimulating factor (GM-CSF; Peprotech cat#: 315-03)
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bioRxiv - Microbiology 2019Quote: ... and 100 to 500 ng/ml of the receptor activator of NF-κB ligand (RANKL; Peprotech). This process was utilized to promote cell differentiation (34 ...
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bioRxiv - Cancer Biology 2023Quote: ... 10 ng/mL IL-3 (Cat#200-03), and 10 ng/mL granulocyte colony-stimulating factor (G-CSF, Cat#300-23) (all from Peprotech, Rock Hill, NJ, USA). Additionally ...
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bioRxiv - Cancer Biology 2023Quote: ... and ligand for fms-like tyrosine kinase receptor-3 (10ng/ul) (all from Peprotech, Cranbury, New Jersey). OP-puro was immediately added to the medium (20uM final concentration ...
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bioRxiv - Immunology 2022Quote: ... CD14+ cells were incubated for 6 days in the presence of 50 ng/ml granulocyte-macrophage colony- stimulating factor (GM-CSF) and 20 ng/ml interleukin-4 (IL-4) (PeproTech®, Neuilly sur Seine, France). The medium used for culture was RPMI ...
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bioRxiv - Immunology 2019Quote: HBD3 concentrations in vaginal lavage was measured by ELISA (PeproTech, London, UK) per manufacturer’s instructions ...
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bioRxiv - Physiology 2021Quote: ... and Human TNF-alpha Stand TMB ELISA (900-T25) was from PeproTech. Lysates were collected for either western blotting or qPCR.
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bioRxiv - Cancer Biology 2022Quote: ... and MDK downregulation or overexpression was determined by ELISA (Peprotech; 900-K190) or qRT-PCR.
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bioRxiv - Immunology 2020Quote: ... Cytokines and chemokines were measured in the cell media supernatant by ELISA (Peprotech). For western blotting ...
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bioRxiv - Bioengineering 2020Quote: ... Growth factor retention was measured using ELISAs (Peprotech, 900-K08 and 900-K10) and a BioTek Synergy 2 luminescent plate reader (Gen5 software).
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bioRxiv - Neuroscience 2021Quote: ... in the presence of 30 ng/mL M-CSF and 100 ng/mL receptor activator of nuclear factor kappa-B ligand (RANKL, PeproTech) (Sousa et al. ...
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bioRxiv - Bioengineering 2022Quote: ... priming medium was replaced by osteoclast medium (priming medium + 50 ng/ml receptor activator of nuclear factor κB ligand (RANKL, 310-01, PeproTech)) to induce osteoclastic differentiation ...
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bioRxiv - Cell Biology 2020Quote: ... Adherent cells were then detached with a cell scrapper and seeded at a density of 5 × 104 cells/cm2 in the presence of 30 ng/mL M-CSF and 100 ng/mL receptor activator of nuclear factor kappa-B ligand (RANKL, PeproTech) (Bartell et al. ...
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bioRxiv - Microbiology 2020Quote: ... which was also combined with 100 or 500 ng/ml of the receptor activator of the NF-κB ligand (RANKL; Peprotech) application to the basolateral media where indicated.
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bioRxiv - Bioengineering 2023Quote: ... After 2 days the coculture medium was replaced by coculture medium additionally containing 50 ng/mL receptor activator of NFκ-B ligand (RANKL, 310-01, PeproTech). Medium was changed weekly inside the incubator via the self-healing injection ports by removing 3 mL of the total 6 mL and replacing it by 3 mL fresh coculture medium with double the concentration of dexamethasone ...
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bioRxiv - Microbiology 2019Quote: ... and 10 healthy controls (sampled once each) were quantified by two-step sandwich ELISA following the manufacturer’s instructions (Peprotech Human BD-2 and BD-3 ...
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bioRxiv - Synthetic Biology 2020Quote: ... cells were spun down and supernatants were collected and analyzed by ELISA for IL-7 following the manufacturer’s recommended protocol (Peprotech).
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bioRxiv - Cell Biology 2020Quote: ... Quantification of released BMP-2 was accomplished using a α-BMP-2 enzyme-linked immunosorbent (ELISA) assay (Peprotech, Hamburg, Germany) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2019Quote: ... The concentration of TNF in cell culture supernatants was quantified by enzyme-linked immunosorbent assay (ELISA) following the instructions provided by the manufacturer (Peprotech, UK). Absorbance at 405 nm ...
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bioRxiv - Cancer Biology 2019Quote: ... Samples were examined by ELISA capturing with Fc-iNKG2D and detecting with biotinylated rabbit-anti-human IL-2 polyclonal antibody (Peprotech #500-P22BT) followed by incubation with streptavidin-HRP ...
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bioRxiv - Bioengineering 2020Quote: ... Hydrogel release samples were analyzed for IL4 and IL13 concentrations using ABTS sandwich ELISAs according to manufacturer’s instructions (Peprotech, Rocky Hill, NJ).
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bioRxiv - Microbiology 2019Quote: Concentration of IL-8 and CXCL16 were measured in the simplified inflammation model with the Human IL-8 (CXCL-8) and Human CXCL16 Mini ABTS ELISA Development Tests (PeproTech, London, UK) and the ABTS ELISA Buffer Kit (PeproTech ...
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bioRxiv - Immunology 2022Quote: ... Specificity was validated by performing normalized supernatant ELISAs against the extracellular domain of human TNFR2 (Peprotech, 310-12 and in-house production). Flow cytometry phenotyping of hybridoma cells was performed on a BD FACS Aria III ...
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bioRxiv - Cancer Biology 2022Quote: ... Equal amounts of conditioned media were measured using a VEGF-A kit (Peprotech, Rock Hill, NJ) that recognizes human VEGF-A (hVEGF-A ...
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bioRxiv - Cell Biology 2021Quote: ... Each serum was assayed for murine IFN-y in duplicate using an enzyme immunoassay kit (Peprotech, BGK01580).
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bioRxiv - Immunology 2023Quote: ... fixed cells were permeabilised with the Transcription Factor staining kit (eBioscience) for 20min at room temperature and stained for 45min with rabbit anti-mouse RELMα (Peprotech) and biotinylated goat anti-mouse Ym1 (R&D) ...
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bioRxiv - Immunology 2024Quote: ... CD14+ monocytes were isolated from PBMCs using the EasySep Human CD14 Positive Selection Kit (Stem Cell) and cultured with 100U/mL GM-CSF (Peprotech) for 4 days prior to plating for stimulation ...