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Citations for Peprotech :
351 - 398 of 398 citations for Ceramide Synthase 3 CERS3 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... then incubated with anti-RELMα primary antibody (Peprotech, Cat# 500-P214), and subsequently an anti-rabbit secondary antibody (Life technologies) ...
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bioRxiv - Immunology 2020Quote: ... Chemokine oligomers were detected by specific rabbit anti-chemokine antibodies (Peprotech) followed by development with an HRP-conjugated anti-rabbit antibody (Abcam).
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bioRxiv - Cancer Biology 2021Quote: ... The primary antibodies used were: anti-PTHrP (Peprotech, Cat# 500-P276), anti-β-casein (Santa Cruz Cat# 166530) ...
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bioRxiv - Cell Biology 2023Quote: ... and TGFβ neutralizing antibodies were purchased from PeproTech (Rocky Hill, NJ).
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bioRxiv - Cell Biology 2020Quote: ... from serial transplants were thawed and maintained in medium (DMEM with 15% FBS + 100 units/ml penicillin streptomycin and 2mM L-glutamine) and supplemented with 10ng/ml IL-3 (Peprotech, 213-13), 10ng/ml IL-6 (Peprotech 216-1 ...
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bioRxiv - Cancer Biology 2020Quote: ... 6 ng/ml murine Interleukin 3 (mIL3) and 20 ng/ml murine stem cell factor (mSCF) (all from Peprotech, Rocky Hill, NJ), and incubated in 5% CO2 at 37°C ...
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bioRxiv - Immunology 2022Quote: ... Cells were transdifferentiation for 7 days at 60,000 cells/cm2 in medium containing 50 ng/ml IL-3 (200-03 B, Peprotech, New Jersey, USA), 50 ng/ml M-CSF (300-25 B ...
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bioRxiv - Cancer Biology 2020Quote: Tumor cells were seeded in 24-well-plates at a density of 3×105 cells per well and treated either with a serial dilution series of IFNγ (PeproTech, 300-02) (starting at 50 ng/ml in two-fold dilution steps ...
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bioRxiv - Cell Biology 2020Quote: ... were maintained in medium (RPMI with 20% FBS + 100 units/ml penicillin streptomycin and 2mM L-glutamine) and supplemented with 10ng/ml IL-3 (Peprotech, 213-13). For in vivo experiments ...
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bioRxiv - Cell Biology 2022Quote: ... After 24 hours cells were re-plated at 30,000 cells/ml in gelatinized 24-well plates in DMEM complete medium supplemented with IL-3 (Peprotech, Cat#213-13) mCSF-1 (Peprotech ...
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bioRxiv - Cancer Biology 2022Quote: ... cells were starved for 3 h prior to ligand stimulation with EGF (50 ng/mL, 10 min; PeproTech, Rocky Hill, NJ, USA), and were lysed in ice-cold lysis buffer (50 mM Tris-HCl ...
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bioRxiv - Cancer Biology 2023Quote: ... 6 ng/ml murine interleukin 3 (mIL3) and 20 ng/ml murine stem cell factor (mSCF) (all from Peprotech, Rocky Hill, NJ), and incubated at 5% CO2 and 37°C ...
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bioRxiv - Bioengineering 2024Quote: ... Isolated PB-NK cells were expanded with irradiated K562-mb15-41BBL feeder cells (1-3 days before NK cell isolation, 100 Gy) along with 50 IU/mL IL-2 (Peprotech, Cranbury, NJ) and 2 ng/mL IL-15 (Peprotech ...
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bioRxiv - Immunology 2024Quote: ... cells were isolated from bone marrow of C57Bl/6 mice and cultured with granulocyte-macrophage stimulating factor (100ng or 2×103 units per 3×106 cells, Peprotech, Cranbury, NJ) in RPMI-1640 media containing 10% heat inactivated fetal bovine serum ...
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bioRxiv - Neuroscience 2019Quote: ... Cells were then left unstimulated or stimulated to generate A1 polarized astrocytes by adding recombinant rat Il-1α (3 ng/mL, PeproTech, Rocky Hill, NJ), recombinant human TNFα (30 ng/mL ...
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bioRxiv - Cell Biology 2021Quote: ... collected in HBSS+/+ and used as controls (3×105 cells/ml) or were incubated with 10 ng/ml TNFα (PeproTech #AF-300-01A), 10 µg/ml recSema7A Fc (R&D 2068-S7) ...
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bioRxiv - Cancer Biology 2022Quote: ... RRID:CVCL_0161) murine cell lines were grown in RPMI-1640 supplemented with 10% FBS and 10ng/ml murine IL-3 (Peprotech, Cat# 213-13). HEK 293T/17 (ATCC Cat# CRL-11268 ...
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bioRxiv - Immunology 2019Quote: ... the suspension cells were removed and culture in a new flask along with 10ng/ml recombinant murine IL-3 (Peprotech, Rocky Hill, NJ). Cells were passaged twice per week ...
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bioRxiv - Immunology 2019Quote: ... 50μM 2-ME and 100 ng/ml of recombinant murine fms-like tyrosine kinase-3 ligand (Flt3L, PeproTech Inc., Rocky Hill, NJ, USA) for 7days ...
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bioRxiv - Microbiology 2024Quote: To differentiate MUTZ-3 cells into a Langerhans cell phenotype (MUTZ-LC) 2×105 cells/ml MUTZ-3 were exposed to 100ng/ml GM-CSF (PeproTech Cat# 300-03), 10ng/ml TGFβ (PeproTech Cat# 100-21 ...
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bioRxiv - Immunology 2021Quote: ... antibody alone or in combination with IL-4 (20 ng/ml, Peprotech) for up to 1 day ...
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bioRxiv - Immunology 2022Quote: ... Cells were incubated for 3 hours at 37°C in RPMI media supplemented with or without recombinant mouse IL-21 (20 ng/mL, Peprotech cat. 210-21-100). Cells were stimulated with either anti-Igκ and anti-Igλ or anti-CD40 ...
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bioRxiv - Molecular Biology 2022Quote: ... with cytokines (150 ng/ml SCF, PeproTech, Cat 300-07; 150 ng/ml Flt-3, 20 ng/ml TPO, PeproTech Cat AF-300-18) and 1% HEPES (Sigma-Aldrich ...
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bioRxiv - Cancer Biology 2022Quote: Panc1 and 4039 cells grown to 80% confluency over 3 days were stimulated for 30 minutes with 30 ng/mL PDGF (Peprotech Cat. no. 100-00AB), then placed immediately on ice ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Immature iPSC-SNs were maintained in N2 medium supplemented with human growth factors (25 ng/mL NGF-β, 450-01; BDNF, 450-02; GDNF, 450-10; NT-3, 450-03; Peprotech, Cranbury, NJ, USA), and 0.2 mM L-ascorbic acid (A4403 ...
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bioRxiv - Cancer Biology 2023Quote: ... 10 ng/mL IL-3 (Cat#200-03), and 10 ng/mL granulocyte colony-stimulating factor (G-CSF, Cat#300-23) (all from Peprotech, Rock Hill, NJ, USA). Additionally ...
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bioRxiv - Neuroscience 2020Quote: ... the membrane was incubated with anti-mCSF1 antibody (1:1000, PeproTech, 500-P62G) at 4°C overnight ...
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bioRxiv - Immunology 2021Quote: ... All cytokines and antibodies noted above were purchased from PeproTech (Rocky Hill, NJ) and BioXCell (West Lebanon ...
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bioRxiv - Immunology 2022Quote: ... All the cells were labeled with monoclonal anti-human TNFα antibody (Peprotech, USA) and subsequently washed with 1X PBS followed by treatment with FITC-conjugated secondary anti-mouse antibody ...
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bioRxiv - Molecular Biology 2019Quote: ... After 7 days cells were detached using TryplE and seeded on non-gelatinized bacterial dishes for suspension culture at 3 million cells per 75cm2 and cultured in N2B27 containing 10ng/mL EGF and FGF (Peprotech 315-09 and 100-18B). After 3 days floating aggregates were seeded on gelatinized dishes ...
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bioRxiv - Cell Biology 2022Quote: ... i3Neurons were cryo-preserved in i3Neuron media (BrainPhys Neuronal Medium supplemented [StemCell] with 2% B27 [Gibco], 10ng/mL BDNF [PreproTech], 10ng/mL NT-3 [PeproTech], and 1µg/mL Laminin [Corning]) with 10% DMSO added ...
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LRP1 mediates leptin transport by coupling with the short-form leptin receptor in the choroid plexusbioRxiv - Neuroscience 2023Quote: ... The membranes were incubated with antibodies against leptin (Cat#: 500-P68, PeproTech, rebury, NJ), pSTAT3 (Cat# ...
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bioRxiv - Microbiology 2019Quote: ... antibodies in the presence of 50 U/ml IL-2 (PeproTech, Inc., Rocky Hill, NJ). On day 2 of activation ...
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bioRxiv - Cell Biology 2020Quote: ... IL-1β and recombinant TNFα and TGFβ neutralizing antibody were purchased from PeproTech (Rocky Hill, NJ).
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bioRxiv - Immunology 2020Quote: ... Plates were washed extensively with TBS and bound chemokine was detected with specific rabbit anti-chemokine antibodies (Peprotech) and HRP-conjugated anti-rabbit secondary antibody development (Abcam) ...
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bioRxiv - Cancer Biology 2024Quote: ... clone OKT3) and anti-CD28 (eBiosciences, clone CD28.2) antibodies in IL-2-containg medium (0.01 ug/ml, Peprotech) for 4 days ...
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bioRxiv - Cancer Biology 2021Quote: ... Recombinant IFN-γ (300-02) and anti-human IFN-γ antibody (506532) were purchased from PeproTech (Rocky Hill, USA). Etoposide (HY-13629 ...
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bioRxiv - Immunology 2019Quote: ... plated for 40 h on a 96-well PVDF plate pre-coated with IGF-1 capture antibody (Peprotech Inc.) and maintained in islet media at 37°C in 5% CO2 ...
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bioRxiv - Cell Biology 2021Quote: ... These cells were then activated using CD3 and CD28 antibodies in the presence of 100U/ml IL-2 (Peprotech). For reprogramming ...
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bioRxiv - Immunology 2020Quote: ... Strips were washed with TBS-T (TBS, 0.1% Tween-20) and bound protein was detected with specific rabbit anti-chemokine antibodies (Peprotech) followed by an HRP-conjugated anti-rabbit antibody (Abcam ...
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bioRxiv - Immunology 2023Quote: ... in the presence of 2mg/mlaCD28 (clone 37.51, WEHI antibody facility) and 100U/ml recombinant human IL-2 (Peprotech), in 96 well flat-bottom plates at 104 cells per well ...
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bioRxiv - Immunology 2022Quote: ... TCR condition – cells activated with anti-CD3/CD28 antibodies on day 0 and cultured with IL-2 (2 ng/ml, PeproTech); TCR+TCR condition – cells activated with anti-CD3/CD28 on day 0 ...
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bioRxiv - Cancer Biology 2023Quote: ... anti-IL-18 (1 µg/mL, MBL), mIgG1 (1 or 10 µg/mL, R&D) antibodies or IL-1RA (100 ng/mL, Peprotech).
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bioRxiv - Cancer Biology 2023Quote: ... with 100 μl of processed CM in the presence or absence of anti-LIF antibody (4 μg/ml) or recombinant mouse LIF (0.1 μg/ml; Peprotech, 250-02). Spheroids were quantified after 8 d culture.
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bioRxiv - Cancer Biology 2019Quote: ... Samples were examined by ELISA capturing with Fc-iNKG2D and detecting with biotinylated rabbit-anti-human IL-2 polyclonal antibody (Peprotech #500-P22BT) followed by incubation with streptavidin-HRP ...
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bioRxiv - Immunology 2022Quote: ... in 500 μL of D10 medium in 24 well plates were stimulated for 7 days at 37°C with anti-mouse CD3 (1 µg/mL) and CD28 (0.5 mg/mL) antibodies plus mouse IL-6 (20 ng/mL) (Peprotech, Rocky Hill, NJ), and human TGF-β1 (2 ng/mL ...
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bioRxiv - Biochemistry 2022Quote: TNFR2-expressing RamosBlue cells were seeded at 5 × 104 cells/well in 100 μL of medium containing antibodies at the indicated concentrations in the absence or presence of 50 ng/mL TNFα (Peprotech #AF-200-01A). The cells were incubated for 18 h ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... and PTX3 levels were analyzed in duplicate using commercially available antibodies (all from R&D Systems, Minneapolis, MN, USA; except IL-1Ra, Peprotech, RockyHill, NJ, USA) in a 384 format using a combination of a SELMA (Jena ...