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Citations for Peprotech :
101 - 150 of 173 citations for Caspase 12 CASP12 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2024Quote: K562 cells were cultured in 12-well plates and treated with 10 ng/mL interferon beta (PeproTech, #300-02BC) for 24 hours ...
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bioRxiv - Immunology 2024Quote: Cells isolated from adipose tissue were stimulated for 3 h with Phorbol 12-myristate 13-acetate (0.5 µg/ml) (PeproTech) and ionomycin (0.5 µg/ml ...
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bioRxiv - Immunology 2023Quote: ... seeded at 2 ml per well in 12 well flat-bottom plates and treated with C5a (PeproTech, 300-70) or avacopan for 72 hrs ...
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bioRxiv - Developmental Biology 2020Quote: ETV2-ECs and ETV2-SPI1-ECs were cultured on coverslips until day 12 of differentiation with VEGFA (50ng/ml) (cat. 100-20, Peprotech). BALB/c LSECs were cultured for 1.5-2 hours after isolation ...
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bioRxiv - Immunology 2019Quote: ... and were then cultured at 1-2×106/ml in 12 well-plates with differentiation media containing 50 ng/ml Interleukin 4 (IL-4, 200-04 A, Peprotech) and 100 ng/ml granulocyte-monocyte colony stimulating factor (GM-CSF ...
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bioRxiv - Cancer Biology 2019Quote: ... At day 12 cell cultures were enriched with hNCCs and cultured in N2B27 supplemented with bFGF and EGF (both 20ng/ml, Peprotech) and 3 μM CHIR99021.
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bioRxiv - Neuroscience 2020Quote: ... 2×105 cells/well/1mL AM medium were seeded on 12-well plates and were treated with 10 ng/ml IL-1β (Peprotech) for 24 hours ...
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bioRxiv - Cancer Biology 2020Quote: Cells were cultured in 96-well plates and treated with 50 μM of Etoposide for 12 h or with 200 ng/mL Trail (Peprotech) and 1 mM MG132 (Sigma) ...
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bioRxiv - Neuroscience 2022Quote: ... Astrocytes were seeded for coculture at a ratio of 1 well of a 6-well plate to 6 wells of a 12-well plate and maintained in E6 medium containing 10 ng/ml CNTF (Peprotech) and 10 ng/ml EGF (Peprotech ...
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bioRxiv - Immunology 2022Quote: ... for Th1 polarization the medium was supplemented with 5 ng/ml IL-2 and 10 ng/ml IL-12 (both from Peprotech), and 10 μg/ml anti-IL-4 (11B11 ...
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bioRxiv - Immunology 2020Quote: ... and were then cultured at 1-2 x 106/ml in 12-well plates with differentiation media containing 50 ng/ml Interleukin 4 (Peprotech) and 100 ng/ml granulocyte-monocyte colony stimulating factor (GM-CSF ...
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bioRxiv - Immunology 2021Quote: ... Activated C7 cells were concentrated and 4 ml of culture was resuspended in 1 ml RPMI+ containing 10 μg/mL ESAT61-20 peptide + 20 ng/mL IL-12 (Peprotech) + 20 ng/mL IL-2 (Peprotech) ...
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bioRxiv - Immunology 2022Quote: Primary neuronal cells (DIV14) or oligodendrocytes were stimulated with 100 ng/μl of recombinant murine IL-12p70 (#210-12, Peprotech) for 5 min ...
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bioRxiv - Immunology 2022Quote: ... primary neuronal cells were stimulated with 30 ng/μl or 100 ng/μl of Recombinant Murine IL-12p70 (#210-12, Peprotech) for 18 hours ...
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bioRxiv - Immunology 2022Quote: ... mice were repeatedly immunized every 14 days (5 times) subcutaneously (s.c.) into the flank with 10μg of human TNFR2 protein (Peprotech, 310-12) and 20μg MPLA (Sigma ...
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bioRxiv - Neuroscience 2023Quote: ... Then NPCs were seeded on Matrigel®-coated flasks at 50 000 cells/cm2 and kept in Astrocyte Induction medium (DMEM/F-12 supplemented with 1x N2 supplement, 1x B27 supplement, 10ng/ml FGF-2 (PeproTech) and 10ng/ml EGF (Miltenyi) ...
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bioRxiv - Immunology 2023Quote: CD11b+ mouse lung cells were seeded in 12-well dishes for 2 days in DMEM supplemented with M-Csf (315-02, Peprotech). Cells were stimulated with murine IL-6 for 72 hours ...
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bioRxiv - Immunology 2023Quote: ... 3×105 CD14+ monocytes were seeded in a 12-well plate in 1 mL complete medium containing 20 ng/mL GM-CSF (#300-03; Peprotech) or 50 ng/mL M-CSF (#300-25 ...
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bioRxiv - Immunology 2021Quote: ... a 6-week-old C57BL/6 mouse was repeatedly immunized 5 times every other week subcutaneously (s.c.) into the flank with 10ug of human TNFR2 protein (Peprotech, 310-12)/20ug MPLA (Sigma ...
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bioRxiv - Cell Biology 2022Quote: ... Activated cells were incubated for 5 days in the presence of the following: IL-12 (25 ng/ml, PeproTech, London, UK) and neutralizing antibody anti–IL-4 (1 μg/ml ...
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bioRxiv - Immunology 2023Quote: ... single-cell suspensions of spleens from 10-week-old Foxp3Thy1.1 mice were stimulated for 15 minutes at 37°C with the indicated concentration of recombinant mouse IL2 (PeproTech, 212-12) in serum-free RPMI 1640 ...
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bioRxiv - Pathology 2023Quote: ... we used the established in vivo Treg induction cocktail consisting of recombinant murine IL2 (0.01 ug/gm, Peprotech, Catalog 212-12) complexed to anti-IL2 antibody (0.05 ug/gm ...
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bioRxiv - Bioengineering 2023Quote: ... cells were differentiated at a concentration of 5 x 105 cells/mL with 100 nM phorbol 12-myristate 13-acetate (PMA, PeproTech, 1652981) in complete RPMI media for 24 hours followed by 3 days of resting in complete RPMI media ...
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bioRxiv - Neuroscience 2020Quote: ... Goat anti-mCSF1 antibody (PeproTech) was applied and incubated at room temperature overnight ...
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bioRxiv - Cancer Biology 2021Quote: ... BMCs were cultured in DMEM/F-12 supplemented with 20 ng/mL murine M-CSF (315-02-10, Peprotech, Cranbury, NJ, USA) in a density of 4 × 106/mL ...
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bioRxiv - Cancer Biology 2022Quote: Single-cell suspensions of cell lines were suspended at a density of 2,000 cells/ml in DMEM/Ham’s F-12 (nacalai tesque, no. 11581-15) containing 20 ng/ml bFGF (Peprotech, no. 100-18B), 20 ng/ml EGF (Wako ...
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A critical role for heme synthesis and succinate in the regulation of pluripotent states transitionsbioRxiv - Developmental Biology 2022Quote: ... 33010-018)-coated plates at a density of 30 000 cells/cm2 and by supplementing the N2B27 medium with 12 ng/ml of bFGF (Peprotech, 100-18B) and 20 ng/ml of activin A (Peprotech ...
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bioRxiv - Immunology 2021Quote: ... Activation was carried out at a cell density of 1M/ml in 100 U/ml Recombinant Murine IL-2 (Peprotech 212-12). Dynabeads™ Mouse T-Activator CD3/CD28 (ThermoFisher 11453D ...
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bioRxiv - Immunology 2022Quote: ... same number of magnetically sorted naive T cells were co-cultured with equal numbers of BMDCs supplemented with 20 ng/ml of recombinant mouse IL-2 (PEPROTECH: 212-12) for 24-72 hours ...
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bioRxiv - Immunology 2022Quote: ... Specificity was validated by performing normalized supernatant ELISAs against the extracellular domain of human TNFR2 (Peprotech, 310-12 and in-house production). Flow cytometry phenotyping of hybridoma cells was performed on a BD FACS Aria III ...
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bioRxiv - Cell Biology 2023Quote: ... cells were washed 3 times with PBS and incubated in naïve dedifferentiation medium of mTeSR-1 supplemented with 12 ng/mL bFGF (Peprotech #AF-100-18B), 1 µM PD0325901 (MEKi ...
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bioRxiv - Neuroscience 2023Quote: ... this medium was switched to N2 medium (Advanced DMEM/F-12, 4% FBS, 1% N2, 1% Penicillin-Streptomycin) supplemented with 10 ng/mL CNTF (PeproTech, 450-13). After 12 days ...
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bioRxiv - Neuroscience 2023Quote: ... cells were expanded in N2 medium (Advanced DMEM/F-12, 4% FBS, 1% N2, 1% Penicillin-Streptomycin) supplemented with 10 ng/mL CNTF (PeproTech, 450-13), which was switched to 10 ng/mL EGF after 12 days ...
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bioRxiv - Immunology 2023Quote: ... A total of 1.5×106/well CD14+ monocytes were seeded in a 12-well plate with 50 ng/mL of hM-CSF (Peprotech, 300-25). On day 6 ...
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bioRxiv - Neuroscience 2020Quote: ... NEM was replaced by astrocyte differentiation medium (DMEM/F-12, 5ng/ml BMP2, 5ng/ml CNTF, and 10 ng/ml bFGF (PeproTech, Rock Hill, NJ)) ...
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bioRxiv - Immunology 2022Quote: ... The following cytokines were added at the indicated concentrations for murine γδT cells: recombinant murine IL-12 (10ng/ml) (Peprotech, Rocky Hill, NJ), IL-15 (50ng/ml ...
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bioRxiv - Immunology 2020Quote: ... All primary anti-chemokine antibodies used in this study were purified polyclonal rabbit antibodies from Peprotech. Recombinant human MFG-E8 protein produced in mouse myeloma cells was purchased from R&D Systems (Minneapolis ...
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bioRxiv - Immunology 2020Quote: ... T cells were cultured in the aforementioned medium supplemented with 1ug/ml OVA Peptide (323-339) and 50U/ml mIL-2 (Peprotech, Cat. No. 212-12). On DAY4 OTII cells were transduced with C1qbp lentiviral particles (Lenti ORF ...
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bioRxiv - Neuroscience 2021Quote: ... Primary antibody for CXCL10 (Peprotech 1:10,000) was incubated at 4 degrees overnight in 10% normal goat serum ...
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bioRxiv - Genetics 2022Quote: ... clone 145-2C11, WEHI antibody facility), anti-CD28 (2μg/ml, clone 3751, WEHI antibody facility) and human IL-2 (Peprotech). CD8 T cell cultures contained 25μg/ml anti-mouse IL2 (clone S4B6 ...
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bioRxiv - Immunology 2023Quote: ... The following anti-mouse primary antibodies were used: anti-RELM-beta (1:500, rabbit polyclonal antibody, Peprotech, 500-p215), anti-MUC2 (1:200 ...
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bioRxiv - Immunology 2023Quote: ... Primary antibodies (RELMβ (1:200, Peprotech 500-p215)) were diluted in the same blocking buffer and incubated with the organoids over night at 4 °C ...
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bioRxiv - Neuroscience 2023Quote: ... Primary antibodies: TDP-43 (Peprotech 10782-2-AP); GAPDH (Millipore Cat# MAB374 ...
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bioRxiv - Cancer Biology 2019Quote: ... using 5 μg/mL of the monoclonal anti-mouse IFN-γ antibody (eBioscience) for capture and 1 μg/mL of the polyclonal biotinylated anti-mouse IFN-γ antibody (PeproTech) for detection.
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bioRxiv - Immunology 2022Quote: ... following the manufacturer protocol, after which straining for ARG1 antibody staining (eBioscience, clone: A1exF5) and RELMα primary antibody staining (Peprotech Cat# 500-P214) proceeded ...
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bioRxiv - Immunology 2021Quote: ... we injected 32 μg anti-CCL5 antibody (500-P118, Peprotech) in 200 μl PBS per mouse intraperitoneally ...
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bioRxiv - Immunology 2022Quote: ... αCD28 (0.5μg/ml) antibodies and rhIL-2 (100U/ml) (PeproTech) at a seeding density of 1×106 cells/ml ...
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bioRxiv - Immunology 2023Quote: ... and polyclonal biotinylated rabbit anti-RELMα detection antibodies (all Peprotech) were used according to a previously described protocol [38].
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bioRxiv - Immunology 2021Quote: ... then incubated with anti-RELMα primary antibody (Peprotech, Cat# 500-P214), and subsequently an anti-rabbit secondary antibody (Life technologies) ...
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bioRxiv - Immunology 2020Quote: ... Chemokine oligomers were detected by specific rabbit anti-chemokine antibodies (Peprotech) followed by development with an HRP-conjugated anti-rabbit antibody (Abcam).