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Citations for Peprotech :
401 - 450 of 467 citations for Anti PI 4 kinase II beta Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... Rabbit anti-RELMβ (1:200 dilution, PeproTech), Rabbit anti-MUC2 (1:200 dilution ...
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bioRxiv - Cell Biology 2021Quote: ... purified and cultured with 10% FBS containing GM-CSF (10 ng/mL) and IL 4 (10 ng/mL) (all PeproTech, USA). FBS (10% ...
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bioRxiv - Biophysics 2021Quote: ... after 24 h recombinant human IL-4 (20 ng/ml) and hSCF (100 ng/ml) (both Peprotech, Rocky Hill, NJ, USA) were added ...
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bioRxiv - Microbiology 2021Quote: ... and fresh 10% fetal bovine serum (10% FBS) with GM-CSF (10 ng/mL) and IL-4 (10 ng/mL) (all PeproTech, USA) were added ...
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bioRxiv - Cell Biology 2021Quote: ... Macrophages were then skewed towards the M2 phenotype using recombinant human IL-4 (20 ng/ml) (Peprotech, Cat#AF-200-04), IL-13 (20 ng/ml ...
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bioRxiv - Neuroscience 2022Quote: ... then 4 million cells were seeded per well in 2 ml of EB Medium: complete mTeSR + 0.05 ug/ml BMP-4 (PeproTech, #120-05) + 0.05 ug/ml VEGF (PeproTech ...
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bioRxiv - Neuroscience 2022Quote: ... qNSCs were treated with aNSC media with the addition of 0.5 μg/mL of the BMP-4 antagonist noggin (PeproTech 120-10C) to more reproducibly induce quiescence exit.
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bioRxiv - Immunology 2022Quote: ... Whole mouse splenocytes and human PBMCs were plated at a density of 5×106 cells per well in a Cepallet W-type 24 well microplate (DIC, Japan) and cultured for 4 days with or without mouse/human M-CSF (Peprotech, USA). Surviving macrophages adhered to 24-well plates were detached by lowering the temperature on ice ...
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bioRxiv - Cancer Biology 2022Quote: ... PBMCs were harvested from K3EDTA anti-coagulated whole blood by density gradient centrifugation with Lymphoprep and plated in 12 well plates at 2-4 × 106 cells per well in 10% RPMI + 10 ng/mL human CSF (300-25, Peprotech, UK). Medium was replaced every 2-3 days until adherent populations of macrophages had established ...
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bioRxiv - Immunology 2024Quote: ... together with 10 ng/mL recombinant mouse IL-4 or 3 μg/mL LPS with 10 ng/mL recombinant murine IFN-ψ (Peprotech) and 20 ng/mL recombinant human BAFF or 5 μg/mL anti-CD40 (Bio X Cell ...
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bioRxiv - Immunology 2021Quote: ... All cytokines and antibodies noted above were purchased from PeproTech (Rocky Hill, NJ) and BioXCell (West Lebanon ...
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bioRxiv - Neuroscience 2022Quote: ... rabbit anti-IL-6 (1:1000 Peprotech, USA), and mouse anti-Iba-1 (1:1000 Millipore ...
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bioRxiv - Microbiology 2021Quote: ... 2 μg/mL anti-IL-12 (PeproTech, P154G), 10 ng/mL TGF-β1 (PeproTech ...
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bioRxiv - Microbiology 2022Quote: ... and Rabbit anti-RELMβ (1:100 dilution, PeproTech). DNA was labeled with DAPI (0.5 μg/mL ...
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bioRxiv - Immunology 2022Quote: ... anti-IFNγ and rmIL-12 (2ng/ml, Peprotech). Intracellular levels of T-bet ...
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bioRxiv - Neuroscience 2023Quote: ... rabbit anti-CCL2 (1:100, PeproTech #500-P76), rabbit anti-connexin 43 (1:500 ...
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bioRxiv - Neuroscience 2021Quote: ... Cells were dissociated using 5mM EDTA in HBSS and resuspended in media with B-27 supplement for 4 hours before incubation with recombinant BDNF protein (100nM, Peprotech, #450-02) or vehicle (equal volume of 0.1% BSA in H2O) ...
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bioRxiv - Immunology 2020Quote: ... cells were washed and cultured for a further 4 days in media containing 20 ng/mL recombinant human IL-2 (Peprotech, London, UK). For analysis of effector cytokine production ...
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bioRxiv - Developmental Biology 2019Quote: ... From day 2 to day 4 the medium was additionally supplemented with BMP and TGFβ inhibitors (Noggin, Peprotech and SB431542, Tocris Bioscience). Treatment with MEK inhibitor (PD184352 ...
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bioRxiv - Immunology 2021Quote: ... Cells were cultured at 1×106 cells/ml in complete RPMI-1640 supplemented with 20 ng/ml GM-CSF and 5 ng/ml IL-4 (PeproTech, Cranbury, NJ) for 7 days (Replaced 70% of medium on Day 2 and Day 5) ...
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bioRxiv - Immunology 2020Quote: ... Alternately M2 MΦs were activated as follows: M2a MΦs from stimulation with 20 ng/mL recombinant human IL -4 (PeproTech; Rock Hill, NJ, USA) and 25 ng/mL recombinant human IL-13 (PeproTech ...
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bioRxiv - Cancer Biology 2023Quote: ... cells were cultured in the absence of EGF and FGF and with the addition of either 10 ng/mL BMP-4 (Peprotech; #315-27) or 1% or 2% foetal bovine serum (Gibco ...
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bioRxiv - Neuroscience 2023Quote: ... this medium was switched to N2 medium (Advanced DMEM/F-12, 4% FBS, 1% N2, 1% Penicillin-Streptomycin) supplemented with 10 ng/mL CNTF (PeproTech, 450-13). After 12 days ...
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bioRxiv - Immunology 2023Quote: ... in the presence of 40 ng/ml of granulocyte-macrophage colony-stimulating factor (GM-CSF) and IL-4 (both from Peprotech or Biolegend). Cells were cultured in 24-well plates in DMEM (Gibco) ...
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bioRxiv - Neuroscience 2023Quote: ... cells were expanded in N2 medium (Advanced DMEM/F-12, 4% FBS, 1% N2, 1% Penicillin-Streptomycin) supplemented with 10 ng/mL CNTF (PeproTech, 450-13), which was switched to 10 ng/mL EGF after 12 days ...
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bioRxiv - Bioengineering 2021Quote: ... supplemented with 50 ng/mL FGF2 and 30 ng/mL animal-free recombinant human Bone Morphogenic Protein 4 (BMP4, Peprotech, AF-120-05ET) for 24 hours ...
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bioRxiv - Neuroscience 2021Quote: ... yolk sac embryoid bodies (YS-EBs) were generated by treating EBs with bone morphogenetic protein 4 (BMP4, 50 ng/ml, Peprotech; to induce mesoderm), stem cell factor (SCF ...
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bioRxiv - Neuroscience 2019Quote: ... the yolk sac embryoid bodies (YS-EBs) were generated by treating the EBs with bone morphogenetic protein 4 (BMP4, 50 ng/ml, Peprotech; to induce mesoderm), vascular endothelial growth factor (VEGF ...
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bioRxiv - Cell Biology 2021Quote: ... cells were treated for 4 days with 50 ng/mL of brain-derived growth factor (BDNF) (Peprotech, Rocky Hill, NJ, USA, #450-02B) in serum-depleted EMEM/F12 media supplemented with 1% pen/strep 88,89.
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bioRxiv - Cell Biology 2021Quote: ... Serum-starved cells were pretreated with 20 nM aPC (Hematologic Technologies, #HCAPC-0080) for 4 h and then incubated with 25 ng/mL TNF-α (PeproTech, #300-01A) for 24 h ...
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bioRxiv - Immunology 2023Quote: ... or 12 well plates and treated with 50 ng/mL of IFN-γ (R & D, 485-MI) or 25 ng/mL of IL-4 (PeproTech, 214-14) for 24 hours ...
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bioRxiv - Immunology 2024Quote: ... or 6.5 were either left unstimulated or were polarized toward a pro-resolution (‘M2’) or pro-inflammatory phenotype (‘M1’) by addition of 10 ng/mL IL-4 (PeproTech, CAT#214-14) and 10 ng/mL IL-13 (PeproTech ...
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bioRxiv - Genomics 2024Quote: ... For M2 polarization macrophages were cultured in macrophage media supplemented with 20 ng/ml recombinant human IL-4 (PeproTech, catalog no. 200-04). Cells were harvested after 24 ...
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LRP1 mediates leptin transport by coupling with the short-form leptin receptor in the choroid plexusbioRxiv - Neuroscience 2023Quote: ... The membranes were incubated with antibodies against leptin (Cat#: 500-P68, PeproTech, rebury, NJ), pSTAT3 (Cat# ...
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bioRxiv - Developmental Biology 2020Quote: ... rabbit anti-CXCL14 (0.2 μg/ml, Peprotech, 500-P237), mouse anti-chick β-actin antibody (1:4000 ...
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bioRxiv - Cell Biology 2021Quote: ... anti-CTGF from Santa Cruz (sc-14939) and anti-FGF2 from PeproTech (#500-P18). FGF2 in condioned medium was also analysed by FGF2 V-PLEX ELISA from MSD (#K151MDD).
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bioRxiv - Neuroscience 2021Quote: ... previously coated with anti-mouse CD3 (clone #17A2, PeproTech) and anti-mouse CD28 (clone #37.51 ...
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bioRxiv - Immunology 2021Quote: ... cells isolated from the femur and tibia were cultured at 1 × 106 cells/ml in 10 ml of complete DMEM (cDMEM) supplemented with 4% recombinant mouse GM-CSF (Peprotech, Rocky Hill, NJ, USA) at 37°C in 7.5% CO2 ...
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bioRxiv - Genomics 2021Quote: ... collected and plated at 10,000 per well in 96-well ultra-low attachment plates in 100 mL embryoid body medium (10 mM ROCK inhibitor, 50 ng/mL BMP-4 (Peprotech; Cat. No. 120-05), 20 ng/mL SCF (Peprotech ...
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bioRxiv - Cancer Biology 2023Quote: ... the FGF and EGF mitogens in the media were replaced by 100 ng/ml of BMP4 (Recombinant human BMP-4, PeproTech, Cat# AF-120-05ET) (quiescence media) ...
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bioRxiv - Microbiology 2019Quote: ... antibodies in the presence of 50 U/ml IL-2 (PeproTech, Inc., Rocky Hill, NJ). On day 2 of activation ...
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bioRxiv - Immunology 2019Quote: ... Polyclonal biotynilated anti-mouse Relm-α was purchased from Peprotech, NJ ...
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bioRxiv - Cell Biology 2020Quote: ... IL-1β and recombinant TNFα and TGFβ neutralizing antibody were purchased from PeproTech (Rocky Hill, NJ).
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bioRxiv - Cancer Biology 2022Quote: ... All cytokines for murine macrophage polarization (mIL-4, mIL-13, mIFN-γ, hIFN-γ, hTGF-β, hIL-2, and mGM-CSF) were purchased from PeproTech (Rocky Hill, NJ). KMO inhibitor (UPF648) ...
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bioRxiv - Immunology 2023Quote: ... and biotinylated anti-TNF-α Ab from PeproTech (Thermo Fisher Scientific). BAL was analyzed for IL-6 ...
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bioRxiv - Immunology 2021Quote: ... or purified polyclonal rabbit anti-Relmα (PeproTech Cat# 500-P214-50ug, RRID:AB_1268843) and biotinylated anti-Ym1/2 (polyclonal ...
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bioRxiv - Neuroscience 2022Quote: ... and rabbit-anti IL-1β (1:500, rabbit, PeproTech, Rocky Hill, NJ). All the western blot analysis was performed three to four times ...
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bioRxiv - Immunology 2019Quote: ... plated for 40 h on a 96-well PVDF plate pre-coated with IGF-1 capture antibody (Peprotech Inc.) and maintained in islet media at 37°C in 5% CO2 ...
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bioRxiv - Cell Biology 2021Quote: ... These cells were then activated using CD3 and CD28 antibodies in the presence of 100U/ml IL-2 (Peprotech). For reprogramming ...
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bioRxiv - Immunology 2023Quote: ... in the presence of 2mg/mlaCD28 (clone 37.51, WEHI antibody facility) and 100U/ml recombinant human IL-2 (Peprotech), in 96 well flat-bottom plates at 104 cells per well ...