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Citations for Peprotech :
401 - 420 of 420 citations for 3 3 Bromomethyl phenyl thiophene since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2024Quote: ... cells were isolated from bone marrow of C57Bl/6 mice and cultured with granulocyte-macrophage stimulating factor (100ng or 2×103 units per 3×106 cells, Peprotech, Cranbury, NJ) in RPMI-1640 media containing 10% heat inactivated fetal bovine serum ...
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bioRxiv - Bioengineering 2024Quote: ... Isolated PB-NK cells were expanded with irradiated K562-mb15-41BBL feeder cells (1-3 days before NK cell isolation, 100 Gy) along with 50 IU/mL IL-2 (Peprotech, Cranbury, NJ) and 2 ng/mL IL-15 (Peprotech ...
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bioRxiv - Cancer Biology 2022Quote: ... cells were starved for 3 h prior to ligand stimulation with EGF (50 ng/mL, 10 min; PeproTech, Rocky Hill, NJ, USA), and were lysed in ice-cold lysis buffer (50 mM Tris-HCl ...
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bioRxiv - Cancer Biology 2023Quote: ... 6 ng/ml murine interleukin 3 (mIL3) and 20 ng/ml murine stem cell factor (mSCF) (all from Peprotech, Rocky Hill, NJ), and incubated at 5% CO2 and 37°C ...
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bioRxiv - Neuroscience 2024Quote: ... EBs cell culture media was replaced with X-VIVO 15 media supplemented with 50 ng/ml Flt-3-ligand (PeproTech, 300-19), 50 ng/ml M-CSF (PeproTech ...
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bioRxiv - Immunology 2024Quote: ... bone marrow precursor cells were obtained from flushed femurs and tibias of C57BL/6J mice and cultured at 3*106 cells/ 10 ml in petri dishes in fully supplemented RPMI and rmGM-CSF (200 U/ml; Peprotech, Hamburg, Germany). Medium was exchanged on days 3 and 6 and the cells were cryopreserved on day 7 for further use.56
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bioRxiv - Neuroscience 2024Quote: ... OPCs from each animal were expanded in 3x 175cm2 flasks for 3-4 days in the presence of 10ng/mL platelet derived growth factor-aa (PDGFAA, Peprotech 100-13A). Cells were harvested fresh for each round of experiments and used at the time of first passage ...
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bioRxiv - Cell Biology 2024Quote: ... Monocytes were differentiated into primary human osteoclasts over 10 days, with media refreshed every 2-3 days and receptor activated nuclear factor κB ligand (RANKL, 25 ng/µL (PeproTech, London, UK) added on differentiation day 8 to stimulate osteoclast formation.
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bioRxiv - Cell Biology 2021Quote: ... collected in HBSS+/+ and used as controls (3×105 cells/ml) or were incubated with 10 ng/ml TNFα (PeproTech #AF-300-01A), 10 µg/ml recSema7A Fc (R&D 2068-S7) ...
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bioRxiv - Cancer Biology 2022Quote: ... RRID:CVCL_0161) murine cell lines were grown in RPMI-1640 supplemented with 10% FBS and 10ng/ml murine IL-3 (Peprotech, Cat# 213-13). HEK 293T/17 (ATCC Cat# CRL-11268 ...
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bioRxiv - Microbiology 2024Quote: To differentiate MUTZ-3 cells into a Langerhans cell phenotype (MUTZ-LC) 2×105 cells/ml MUTZ-3 were exposed to 100ng/ml GM-CSF (PeproTech Cat# 300-03), 10ng/ml TGFβ (PeproTech Cat# 100-21 ...
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bioRxiv - Immunology 2024Quote: ... at a 1:3 T cell:bead ratio cultured in R10 media and 20 IU of recombinant human IL-2 (Peprotech, Cat-no. 200-02). Cells were transduced with CAR lentivirus at a multiplicity of infection (MOI ...
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bioRxiv - Immunology 2022Quote: ... Cells were incubated for 3 hours at 37°C in RPMI media supplemented with or without recombinant mouse IL-21 (20 ng/mL, Peprotech cat. 210-21-100). Cells were stimulated with either anti-Igκ and anti-Igλ or anti-CD40 ...
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bioRxiv - Molecular Biology 2022Quote: ... with cytokines (150 ng/ml SCF, PeproTech, Cat 300-07; 150 ng/ml Flt-3, 20 ng/ml TPO, PeproTech Cat AF-300-18) and 1% HEPES (Sigma-Aldrich ...
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bioRxiv - Cancer Biology 2022Quote: Panc1 and 4039 cells grown to 80% confluency over 3 days were stimulated for 30 minutes with 30 ng/mL PDGF (Peprotech Cat. no. 100-00AB), then placed immediately on ice ...
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bioRxiv - Cancer Biology 2023Quote: ... 10 ng/mL IL-3 (Cat#200-03), and 10 ng/mL granulocyte colony-stimulating factor (G-CSF, Cat#300-23) (all from Peprotech, Rock Hill, NJ, USA). Additionally ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Immature iPSC-SNs were maintained in N2 medium supplemented with human growth factors (25 ng/mL NGF-β, 450-01; BDNF, 450-02; GDNF, 450-10; NT-3, 450-03; Peprotech, Cranbury, NJ, USA), and 0.2 mM L-ascorbic acid (A4403 ...
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bioRxiv - Cell Biology 2022Quote: ... i3Neurons were cryo-preserved in i3Neuron media (BrainPhys Neuronal Medium supplemented [StemCell] with 2% B27 [Gibco], 10ng/mL BDNF [PreproTech], 10ng/mL NT-3 [PeproTech], and 1µg/mL Laminin [Corning]) with 10% DMSO added ...
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bioRxiv - Developmental Biology 2024Quote: ... the medium was changed to late hCNCCs medium by supplementing the early hCNCCs medium with 3 µM CHIR 99021 (Selleckchem, catalog no. S2924) and 50 pg/mL BMP2 (Peprotech, catalog no. AF-120-02) to enhance cell proliferation and reduce migration28 ...
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bioRxiv - Molecular Biology 2024Quote: ... cells were detached using TrypLE and seeded on nongelatinized bacterial dishes for suspension culture at 3 million cells per 75 cm2 and cultured in N2B27 containing 10 ng/mL EGF and FGF (Peprotech 315-09 and 100-18B). After 3 days ...