Labshake search
Citations for Peprotech :
351 - 400 of 404 citations for Rat Colony Assay Media since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2024Quote: ... cells were replated as single cells onto poly-D-lysine-laminin-coated plates at 1×106 cells/cm2 for astrogenic switch and progenitor expansion in N2B27 media supplemented with 10 ng/mL FGF2 (Peprotech) and 10 ng/mL Human EGF (Peprotech ...
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bioRxiv - Genomics 2024Quote: ... macrophages were cultured in macrophage media supplemented with 20 ng/ml human recombinant IFN-γ (PeproTech, catalog no. 300-02) and 25 ng/ml LPS (Invivogen ...
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bioRxiv - Biochemistry 2023Quote: ... cells were plated (60,000 cells/well in 24-well plates) two days before treatment in complete media supplemented with 25 ng/mL murine IL-4 (Peprotech) for M2-polarization or without any cytokines (M0) ...
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bioRxiv - Genomics 2023Quote: ... CD34+ HSPCs were differentiated to the megakaryocyte lineage by culturing for 11 days in IMDM based media containing 30ng/mL rhTPO (PeproTech), 1ng/mL rhSCF (PeproTech) ...
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bioRxiv - Immunology 2023Quote: ... beads taken off each well and replated for resting in T cell media containing 10 U/mL of Interleukin-2 (IL-2; Peprotech). To restart each stimulation cycle ...
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bioRxiv - Biophysics 2021Quote: ... bone marrow progenitor cells were cultured in complete RPMI media supplemented with interleukin-4 (IL-4, 10 ng/mL, PeproTech, Canada) and granulocyte-macrophage colony-stimulating factor (GM-CSF ...
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bioRxiv - Molecular Biology 2020Quote: ... NPCs were cultured for up to 5 passages on Matrigel with neural induction media supplemented with 40 ng/ml bFGF (Peprotech: (AF)100-18B) ...
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bioRxiv - Cell Biology 2020Quote: ... The monolayer was washed 3 times with HBSS to remove non-adherent cells and monocytes were differentiated for 3 days in RPMI media containing 20 ng/ml M-CSF and 20ng/ml recombinant human RANKL (PeproTech, UK). After 3 days of culture ...
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bioRxiv - Immunology 2022Quote: ... and differentiated for 7 days in Iscove’s Modified Dulbecco’s Media (IMDM) supplemented with 20 ng/ml of M-CSF (Peprotech, Rocky Hill, NJ), 10% heat-inactivated fetal bovine serum (FBS ...
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bioRxiv - Immunology 2020Quote: ... Rinse solution was then aspirated and replaced with 1 mL of 2X concentrated EB medium (1X EB media: OXE8 medium supplemented with 50 ng/mL BMP4 (Peprotech, #PHC9534), 50 ng/mL VEGF (Peprotech ...
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bioRxiv - Neuroscience 2022Quote: ... qNSCs were treated with aNSC media with the addition of 0.5 μg/mL of the BMP-4 antagonist noggin (PeproTech 120-10C) to more reproducibly induce quiescence exit.
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bioRxiv - Neuroscience 2023Quote: ... the media was changed to promote the neuronal differentiation and it was supplemented with 20 ng/ml BDNF (PeproTech, 450-02), 20 ng/ml NT-3 (Alomone labs ...
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bioRxiv - Immunology 2024Quote: ... Transduced cells were incubated for 4 days with plate-bound anti-CD3/anti-CD28 in media supplemented with hIL-2 (0.0344 units/mL, Peprotech #200-02). Transduction efficiency was assessed for each experiment by flow cytometry.
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bioRxiv - Cell Biology 2022Quote: ... and media were replaced with either pre-warmed SFM or pre-warmed SFM supplemented with 20 ng/mL HGF (PeproTech, 100-39) for 30 min ...
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bioRxiv - Neuroscience 2021Quote: ... Cells were dissociated using 5mM EDTA in HBSS and resuspended in media with B-27 supplement for 4 hours before incubation with recombinant BDNF protein (100nM, Peprotech, #450-02) or vehicle (equal volume of 0.1% BSA in H2O) ...
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bioRxiv - Neuroscience 2021Quote: ... Neural rosettes were selected manually for further expansion on Geltrex coated dishes and grown in neural differentiation media (NDM) (Neurobasal supplemented with NEAA, Glutamax, N2, B27, 1μM cAMP, 10 ng/ml BDNF (Peprotech, Cranbury, NJ. U.S.), 10 ng/ml GDNF (Peprotech ...
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bioRxiv - Immunology 2021Quote: ... cells were starved for 6 hours in fresh media lacking cytokines and subsequently treated with 100 U/mL of either IL-2 or IL-15 (PeproTech 200-15).
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bioRxiv - Microbiology 2020Quote: ... Frozen activated PBMC cells were thawed and resuspended in complete PBMC media containing RPMI supplemented with 21 ng/mL human IL-2 (Peprotech; #200-02), 10% fetal bovine serum and 1% penicillin-streptomycin ...
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bioRxiv - Cell Biology 2021Quote: ... For dendritic cell differentiation the bone marrow was resuspended in 25 ml of complete RPMI 1640 media supplemented with 20 ng/ml GM-CSF (Peprotech #315-03), 10 ng/ml IL-4 (Peprotech #AF-214-14 ...
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bioRxiv - Immunology 2020Quote: ... cells were washed and cultured for a further 4 days in media containing 20 ng/mL recombinant human IL-2 (Peprotech, London, UK). For analysis of effector cytokine production ...
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bioRxiv - Immunology 2022Quote: ... primary BMDMs were grown for 7 days in complete RPMI media containing 20 ng/mL of recombinant MCSF (Peprotech, Rocky Hill, NJ) prior to use in assays ...
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bioRxiv - Cell Biology 2021Quote: ... Purified microglia cells were span down at 300 × g and plated at 100 103 cells per well in microglia media supplemented with 100 ng/ml of rhIL34 (Peprotech, 200-34), 2 ng/ml TGFb2 (Peprotech ...
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bioRxiv - Biochemistry 2022Quote: ... cells were diluted to a density of 6e4/mL and 50 μL (3e3 cells) were added to 50 μL of media containing 2x of a serial dilution of human CSF3 (PeproTech 300-23) in a 96-well plate ...
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bioRxiv - Neuroscience 2022Quote: ... hNPCs were plated on poly-L-ornithine- and laminin-coated plates and were differentiated with growth media (without FGF-b) supplemented with 25ng/mL recombinant human glial-derived neurotrophic factor (GDNF; PeproTech US, NJ). Fifty percent of cell media were removed every three to four days and replaced with fresh GDNF supplemented media ...
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bioRxiv - Neuroscience 2023Quote: ... cells were plated (15,000 cells/well) on gelatincoated 6-well TC dishes in AK media + EGF (Peprotech 315-09, 20 ng/mL), FGF1 (Peprotech 450-33 ...
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bioRxiv - Bioengineering 2023Quote: ... OPCs were cultured using OPC maintenance/proliferation media (OMM) composed of N2B27 supplemented with 10ng/ml insulin-like growth factor (IGF-1; Peprotech, 100-11), 15ng/ml platelet-derived growth factor AA (PDGFaa ...
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bioRxiv - Bioengineering 2023Quote: ... M1-like MØs were obtained by culturing M0 MØs in RPMI media supplemented with 10pg/ml Lipopolysaccharides (MilliporeSigma, MO, USA) and 1 ng/ml human Interferon-gamma (Peprotech, NJ, USA). Protocol for generating M0 ...
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bioRxiv - Neuroscience 2023Quote: ... cells were resuspended in 320 μl of fresh DRG media (F12, 10% hi-FBS, 1% Pen/Strep, 20 ng/ml mouse GDNF (Peprotech #450-44), 50 ng/ml mouse NGF (Peprotech #450-34) ...
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bioRxiv - Immunology 2023Quote: ... TIL or KSQ-001 cells were added to spheroid plate in 100μL REP media supplemented with 6000IU/mL IL-2 (Peprotech, Cat#200-02). Images were taken via Incucyte S3 (Sartorius ...
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bioRxiv - Cancer Biology 2023Quote: Primary human AML specimens were cultured as previously described using InVitria Metabolomics AF media supplemented 10nM human cytokines SCF (PEPROTech, 300-07), IL3 (PEPROTech ...
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bioRxiv - Cell Biology 2024Quote: ... the plating medium was removed and replaced with complete media used for pHi manipulations (see section above) or Wnt stimulation (40 ng/mL (Cf) murine WNT3a (Peprotech; 315-20)) ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... and prostaglandin E2 (PGE2) enzyme-linked immunosorbent assay (ELISA) kit were purchased from Peprotech (NSW, Australia). Dulbecco’s Modified Eagle Medium (DMEM ...
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Nanoparticle-delivered TLR4 and RIG-I agonists enhance immune response to SARS-CoV-2 subunit vaccinebioRxiv - Immunology 2022Quote: ... RBC-lysed murine bone marrow was seeded at 2 million cells/mL in complete RPMI media with 200 ng/mL human FLT3 ligand (PeproTech, Cat# 300-19) in a 6-well plate (10 million cells/well) ...
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bioRxiv - Physiology 2019Quote: ... Cells were then serum starved in DMEM culture media with 2% BGS and the conditioned culture was supplemented with 200ng/ml of murine IL-10 recombinant protein (Peprotech, Rocky Hill, NJ). After 48 hours ...
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bioRxiv - Cell Biology 2021Quote: ... For macrophage differentiation the bone marrow was resuspended in 25 ml of complete RPMI 1640 media supplemented with 20 ng/ml M-CSF (Peprotech #AF-315-02) and 50 ng/ml Amphotericin B ...
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bioRxiv - Immunology 2021Quote: ... were plated with the irradiated 40LB cells in R10 media with 1 ng/mL recombinant mouse interleukin-4 (rmIL-4; Peprotech, East Windsor, NJ). On day 4 ...
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bioRxiv - Cancer Biology 2022Quote: ... All lung NET organoids and some LCNEC organoids were grown in media additionally supplemented with 0.05 μg/ml EGF (Peprotech, Cat# AF-100-15). Media was changed once a week.
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bioRxiv - Bioengineering 2023Quote: ... NPSCs were expanded using NPSC proliferation media (NPM) composed of N2B27 supplemented with 20 ng/ml epidermal growth factor (EGF; Peprotech, AF-100-15), 20ng/ml basic fibroblast growth factor (bFGF ...
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bioRxiv - Cancer Biology 2023Quote: ... the old serum-free medium was replaced with new serum-free media with or without 100 ng/mL EGF (Peprotech AF-100-15). For each experimental condition (i.e. ...
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bioRxiv - Neuroscience 2023Quote: ... The last 3 days in culture involve feeding in iMGL maturation media, which is iMGL medium freshly supplemented with 100ng/mL CD200 (Novoprotein, cat#BP004) and 100 ng/mL CX3CL1 (Peprotech, cat#300-18). Mature iMGL were treated with LPS (ThermoFisher Scientific ...
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bioRxiv - Genomics 2024Quote: ... For M2 polarization macrophages were cultured in macrophage media supplemented with 20 ng/ml recombinant human IL-4 (PeproTech, catalog no. 200-04). Cells were harvested after 24 ...
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bioRxiv - Developmental Biology 2024Quote: ... proteins were supplemented in Maturation media by dissolving Matrigel at 1% (v/v) containing human recombinant brain-derived neurotrophic factor (BDNF; PeproTech, AF-450-02). The aggregated cells were referred to as cerebral organoids from this stage onward and were allowed to further develop in maturation media until the experimental endpoints ...
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bioRxiv - Immunology 2022Quote: ... Cells were incubated for 3 hours at 37°C in RPMI media supplemented with or without recombinant mouse IL-21 (20 ng/mL, Peprotech cat. 210-21-100). Cells were stimulated with either anti-Igκ and anti-Igλ or anti-CD40 ...
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bioRxiv - Genomics 2021Quote: ... Microglia progenitors were harvested from suspension after 14-21 days and plated onto PDL-coated plates in microglia maturation media (2 mM GlutaMax, 1x Antibiotic-Antimycotic, 100 ng/mL IL-34 (Peprotech; Cat. No. 200-34), and 10 ng/mL GM-CSF (Peprotech ...
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bioRxiv - Cancer Biology 2023Quote: ... the FGF and EGF mitogens in the media were replaced by 100 ng/ml of BMP4 (Recombinant human BMP-4, PeproTech, Cat# AF-120-05ET) (quiescence media) ...
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bioRxiv - Cell Biology 2020Quote: ... They were grown under standard conditions at 37°C and 5% CO2 with NeuroCult basal media (STEMCELL, Vancouver, BC, CA) with EGF (50 ng/ml, PeproTech, Inc., Rocky Hill, NJ, USA), FGF (100 ng/ml ...
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bioRxiv - Immunology 2020Quote: ... The cells were then centrifuged and the cell pellet resuspended in RPMI-1640 supplemented media with 300U/mL rhIL-2 (Peprotech, 200-02 and StemCell, 78036.3), 100 ng/mL of anti-CD3 (eBiosciences ...
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bioRxiv - Cell Biology 2022Quote: ... i3Neurons were cryo-preserved in i3Neuron media (BrainPhys Neuronal Medium supplemented [StemCell] with 2% B27 [Gibco], 10ng/mL BDNF [PreproTech], 10ng/mL NT-3 [PeproTech], and 1µg/mL Laminin [Corning]) with 10% DMSO added ...
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bioRxiv - Cell Biology 2020Quote: ... Quantification of released BMP-2 was accomplished using a α-BMP-2 enzyme-linked immunosorbent (ELISA) assay (Peprotech, Hamburg, Germany) according to the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2022Quote: ... All the supernatants were evaluated by using a human FGF-basic standard ABTS enzyme-linked immunosorbent assay (ELISA) development kit and ABTS ELISA buffer kit (both Peprotech).