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Citations for Peprotech :
351 - 400 of 1717 citations for 1 Piperidineacetamide 4 2 benzothiazolyl N 4 4 morpholinyl phenyl 9ci since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2024Quote: ... 100 units/mL penicillin and 100 μg/mL streptomycin] containing IL-4 (20 ng/mL) and GM-CSF (20 ng/ml) (PeproTech) and cultured for 5d with replacement of half of the medium and addition of fresh cytokines every other day ...
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bioRxiv - Developmental Biology 2024Quote: ... 3 μM CHIR treatment was performed from day 4 alongside 50ng/ml Fgf2 (Murine FGF-basic, Peprotech, 450-33-100). CHIR treatment was maintained for two days while Fgf2 was maintained for three additional days.
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bioRxiv - Immunology 2021Quote: ... Th17 cell cultures received 5 ng/mL TGF-β and 50 ng/mL IL-6 recombinant and 20 µg/mL anti-IL-4 and 100 µg anti-IFN-γ (PeproTech). After 48 h ...
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bioRxiv - Cell Biology 2021Quote: ... purified and cultured with 10% FBS containing GM-CSF (10 ng/mL) and IL 4 (10 ng/mL) (all PeproTech, USA). FBS (10% ...
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bioRxiv - Biophysics 2021Quote: ... after 24 h recombinant human IL-4 (20 ng/ml) and hSCF (100 ng/ml) (both Peprotech, Rocky Hill, NJ, USA) were added ...
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bioRxiv - Microbiology 2021Quote: ... and fresh 10% fetal bovine serum (10% FBS) with GM-CSF (10 ng/mL) and IL-4 (10 ng/mL) (all PeproTech, USA) were added ...
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bioRxiv - Cell Biology 2021Quote: ... Macrophages were then skewed towards the M2 phenotype using recombinant human IL-4 (20 ng/ml) (Peprotech, Cat#AF-200-04), IL-13 (20 ng/ml ...
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bioRxiv - Neuroscience 2022Quote: ... qNSCs were treated with aNSC media with the addition of 0.5 μg/mL of the BMP-4 antagonist noggin (PeproTech 120-10C) to more reproducibly induce quiescence exit.
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bioRxiv - Immunology 2022Quote: ... Whole mouse splenocytes and human PBMCs were plated at a density of 5×106 cells per well in a Cepallet W-type 24 well microplate (DIC, Japan) and cultured for 4 days with or without mouse/human M-CSF (Peprotech, USA). Surviving macrophages adhered to 24-well plates were detached by lowering the temperature on ice ...
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bioRxiv - Immunology 2024Quote: ... together with 10 ng/mL recombinant mouse IL-4 or 3 μg/mL LPS with 10 ng/mL recombinant murine IFN-ψ (Peprotech) and 20 ng/mL recombinant human BAFF or 5 μg/mL anti-CD40 (Bio X Cell ...
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bioRxiv - Cancer Biology 2023Quote: ... with 100 μl of processed CM in the presence or absence of anti-LIF antibody (4 μg/ml) or recombinant mouse LIF (0.1 μg/ml; Peprotech, 250-02). Spheroids were quantified after 8 d culture.
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bioRxiv - Immunology 2024Quote: ... was used for sensitizing the cells in the presence of recombinant human IL-4 (Gibco, PeproTech, #200-04; 10 ng/mL) for three days ...
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bioRxiv - Cell Biology 2024Quote: ... centrifuged and resuspended in satellite cell growth medium (SCGM: F-10 Ham’s medium supplemented with 20% FBS, 4 ng/ ml FGF2 (PeproTech; 100-18B), 1% penicillin–streptomycin and 10mM HEPEs) ...
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bioRxiv - Neuroscience 2021Quote: ... Cells were dissociated using 5mM EDTA in HBSS and resuspended in media with B-27 supplement for 4 hours before incubation with recombinant BDNF protein (100nM, Peprotech, #450-02) or vehicle (equal volume of 0.1% BSA in H2O) ...
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bioRxiv - Immunology 2021Quote: ... Cells were cultured at 1×106 cells/ml in complete RPMI-1640 supplemented with 20 ng/ml GM-CSF and 5 ng/ml IL-4 (PeproTech, Cranbury, NJ) for 7 days (Replaced 70% of medium on Day 2 and Day 5) ...
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bioRxiv - Immunology 2020Quote: ... Alternately M2 MΦs were activated as follows: M2a MΦs from stimulation with 20 ng/mL recombinant human IL -4 (PeproTech; Rock Hill, NJ, USA) and 25 ng/mL recombinant human IL-13 (PeproTech ...
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bioRxiv - Immunology 2020Quote: ... and Th17 (anti-IFN-γ-10μg/ml, anti-IL-4–10μg/ml, IL-6-Peprotech,20ng/ml, TGF-β-Peprotech,2ng/ml) polarizing conditions for 4 days ...
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bioRxiv - Immunology 2020Quote: ... and Th17 (anti-IFN-γ-10μg/ml, anti-IL-4–10μg/ml, IL-6-Peprotech,20ng/ml, TGF-β-Peprotech,2ng/ml) polarizing conditions for 4 days ...
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bioRxiv - Cancer Biology 2023Quote: ... cells were cultured in the absence of EGF and FGF and with the addition of either 10 ng/mL BMP-4 (Peprotech; #315-27) or 1% or 2% foetal bovine serum (Gibco ...
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bioRxiv - Immunology 2023Quote: ... in the presence of 40 ng/ml of granulocyte-macrophage colony-stimulating factor (GM-CSF) and IL-4 (both from Peprotech or Biolegend). Cells were cultured in 24-well plates in DMEM (Gibco) ...
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bioRxiv - Systems Biology 2024Quote: ... Osteogenic differentiation was initiated on Day 0 by culturing in osteogenic differentiation medium consisting of growth medium and 100 ng/mL bone morphogenetic protein-4 (BMP4) (PeproTech, 315-27). The osteogenic differentiation medium was replaced every second day.
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bioRxiv - Neuroscience 2024Quote: ... OPCs from each animal were expanded in 3x 175cm2 flasks for 3-4 days in the presence of 10ng/mL platelet derived growth factor-aa (PDGFAA, Peprotech 100-13A). Cells were harvested fresh for each round of experiments and used at the time of first passage ...
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bioRxiv - Bioengineering 2021Quote: ... supplemented with 50 ng/mL FGF2 and 30 ng/mL animal-free recombinant human Bone Morphogenic Protein 4 (BMP4, Peprotech, AF-120-05ET) for 24 hours ...
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bioRxiv - Neuroscience 2021Quote: ... yolk sac embryoid bodies (YS-EBs) were generated by treating EBs with bone morphogenetic protein 4 (BMP4, 50 ng/ml, Peprotech; to induce mesoderm), stem cell factor (SCF ...
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bioRxiv - Cell Biology 2021Quote: ... cells were treated for 4 days with 50 ng/mL of brain-derived growth factor (BDNF) (Peprotech, Rocky Hill, NJ, USA, #450-02B) in serum-depleted EMEM/F12 media supplemented with 1% pen/strep 88,89.
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bioRxiv - Cell Biology 2021Quote: ... Serum-starved cells were pretreated with 20 nM aPC (Hematologic Technologies, #HCAPC-0080) for 4 h and then incubated with 25 ng/mL TNF-α (PeproTech, #300-01A) for 24 h ...
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bioRxiv - Immunology 2023Quote: ... or 12 well plates and treated with 50 ng/mL of IFN-γ (R & D, 485-MI) or 25 ng/mL of IL-4 (PeproTech, 214-14) for 24 hours ...
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bioRxiv - Neuroscience 2024Quote: ... were generated from hiPSCs and induced to yolk sac EBs (YS-EBs) by adding bone morphogenetic protein 4 (BMP4, 50 ng/mL, Peprotech; to induce mesoderm), vascular endothelial growth factor (VEGF ...
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bioRxiv - Immunology 2024Quote: ... 1×105 BMDM were added to iDpt;R26YFP-CTRL and iDpt;R26YFP;Csf1fl/f Exons 4–6 fibroblasts or plated in the presence/absence of M-CSF (Peprotech; Cat# 315-02). The 96-well plate was placed on the Incucyte SX5 (Sartorius) ...
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bioRxiv - Immunology 2023Quote: ... After 2 days of stimulation and resting o/n in fresh medium containing 10 ng/ml rhIL-2 (Peprotech), T cell were labelled in modified RPMI 1640 containing 1 g/l 13C6-glucose (Sigma) ...
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bioRxiv - Genomics 2021Quote: ... collected and plated at 10,000 per well in 96-well ultra-low attachment plates in 100 mL embryoid body medium (10 mM ROCK inhibitor, 50 ng/mL BMP-4 (Peprotech; Cat. No. 120-05), 20 ng/mL SCF (Peprotech ...
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bioRxiv - Cancer Biology 2023Quote: ... the FGF and EGF mitogens in the media were replaced by 100 ng/ml of BMP4 (Recombinant human BMP-4, PeproTech, Cat# AF-120-05ET) (quiescence media) ...
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bioRxiv - Neuroscience 2024Quote: ... NPC were plated on poly- L-ornithine/laminin-coated flasks at 50’000 cells/cm2 in2µg/ml laminin-supplemented neural expansion medium (DMEM/F-12 + Glutamax, Gibco®; 1x N-2 supplement, Gibco®; 1x B27 supplement without vitamin A, Gibco®; 10 ng/ml FGF-2, PeproTech; 10 ng/ml EGF, Miltenyi). NPC were then transduced with a VSV-G-pseudotyped lentivirus (carrying pCW57.1 plasmid from Addgene # 41393 (gift from David Root ...
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bioRxiv - Neuroscience 2023Quote: ... murine IL-2 (1 μg; Peprotech) was mixed with anti-mouse IL-2 (5 μg ...
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bioRxiv - Neuroscience 2020Quote: ... and 1% insulin transferrin selenium (Cat N° 41400045) in IMDM (Cat N° 12440053) supplemented with 5 ng/ml recombinant human basic fibroblast growth factor (bFGF) (Peprotech, Cat N° 100-18B). Medium was changed every 4 days ...
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bioRxiv - Bioengineering 2021Quote: ... and fibroblast growth factor 2 (FGF-2, 5 ng mL-1; PeproTech). Cells were passaged before reaching 90% confluency and the medium was changed every 2–3 days.
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bioRxiv - Bioengineering 2024Quote: ... Noggin (“N,” 100 ng/mL, PeproTech), R-spondin-conditioned medium (“R,” 5% v/v ...
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bioRxiv - Bioengineering 2021Quote: ... and 10 ng mL−1 FGF-2 (PeproTech). For immunocytochemistry ...
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bioRxiv - Immunology 2020Quote: ... 1 μg of recombinant murine IL-2 (PeproTech) was combined with 10 μg anti-mouse IL-2 (clone S4B6 ...
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bioRxiv - Cell Biology 2023Quote: ... and FGF-2 (50 ng mL-1, Peprotech). For the initial period of culture ...
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bioRxiv - Bioengineering 2021Quote: ... IGF-2 (30 ng ml-1, PeproTech, 100-12), and EGF (50 ng ml-1 ...
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bioRxiv - Neuroscience 2022Quote: ... 1% Glutamax) containing 20 ng/mL FGF-2 (PeproTech) and 20 ng/mL EGF (R&D Systems) ...
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bioRxiv - Immunology 2024Quote: ... with human recombinant IL-2 (1 ng/ml, Peprotech), IL-7 (5 ng/ml ...
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bioRxiv - Synthetic Biology 2024Quote: ... and 1% recombinant human IL-2 (Peprotech, 200-02). All cells were maintained in a humidified incubator at 37°C with 5% CO2 and passaged as per standard protocols to ensure optimal growth and experimental conditions.
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bioRxiv - Immunology 2024Quote: ... at a 1:1 ratio and recombinant human IL-2 (Peprotech, ThermoFisher Scientific), and expanded in RPMI 1640 medium supplemented with 10% fetal bovine serum (FBS) ...
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bioRxiv - Bioengineering 2024Quote: ... 1 ng/mL human fibroblast growth factor 2 (FGF-2) (PeproTech #100-18B, Rocky Hill, NJ, USA), supplemented with 1 % antibiotic/antimycotic (ThermoFisher #1540062 ...
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bioRxiv - Developmental Biology 2022Quote: ... 2 ng/mL insulin-like growth factor 1 (IGF1) (Peprotech), and 10 ng/mL hepatocyte growth factor (HGF ...
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bioRxiv - Bioengineering 2024Quote: ... rhIL-2 (20 ng ml-1, Cat: 200-02, Peprotech) and rhIL-7 (5 ng ml-1) ...
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bioRxiv - Immunology 2024Quote: ... at 1:1 beads-to-cells ratio supplemented with 30U/ml human IL-2 (Peprotech).
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bioRxiv - Immunology 2024Quote: ... and 50 U ml−1 rhIL-2 (cat. 200-02, Peprotech). On day 2 post activation ...