Labshake search
Citations for Peprotech :
351 - 400 of 1012 citations for 1 4 Nitrophenyl cyclobutanecarbonitrile since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: 750 000 MCs were either FACS-sorted after 24h of coculture with resting or anti-CD3/28 coated beads-activated effector/memory CD4+ T cells or stimulated for 4 hours with 10 ng/mL of IL-33 (Peprotech) or with 100 ng/mL of DNP-BSA (thermo-fischer scientific) ...
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bioRxiv - Immunology 2023Quote: ... were cultured in six-well plates (2 ml per well) in complete RPMI 1640 medium + 10% FBS + 10 ng/ml human IL-4 (PeproTech) + 100 ng/ml human GM-CSF (PeproTech) ...
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bioRxiv - Immunology 2022Quote: Single bone marrow cell suspensions were plated at 4×106 per well in 2 mL of complete IMDM media supplemented with 150 ng/mL of FLT3L (PeproTech). On day 7 of differentiation ...
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bioRxiv - Immunology 2022Quote: ... Th2 cells were generated by activating naïve CD4+ T cells with 1 μg/mL anti-CD3ε/3 μg/mL anti-CD28 for 3 days in complete T cell medium supplemented with 250U recombinant IL-4 (PeproTech), 6 μg/mL anti-IL-12 (BioXcell) ...
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bioRxiv - Immunology 2024Quote: ... R&D), IL-27 (20 ng/mL, R&D) as well as with 2 μg/mL anti-mouse IL-4 (Peprotech). Alternatively ...
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bioRxiv - Biochemistry 2023Quote: ... cells were plated (60,000 cells/well in 24-well plates) two days before treatment in complete media supplemented with 25 ng/mL murine IL-4 (Peprotech) for M2-polarization or without any cytokines (M0) ...
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bioRxiv - Immunology 2023Quote: ... Cultures were changed on day 4 to new complete RPMI 1640 medium containing recombinant murine IL-5 (10 ng/ml, Peprotech) and further fed with IL-5 every other day between days 10 to 14 ...
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bioRxiv - Immunology 2022Quote: ... or into bone marrow-derived dendritic cells (BMDC) in cRPMI supplemented with recombinant mouse IL-4 and GM-CSF (Peprotech). Influenza virus strains A/California/04/2009 (H1N1) ...
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bioRxiv - Molecular Biology 2024Quote: ... followed by an additional 4 hours with or without an apoptotic stimulus of 10 ng/ml TNFα (Peprotech, Cranbury, NJ) and 20 μg/mL CHX ...
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bioRxiv - Immunology 2024Quote: ... 100 units/mL penicillin and 100 μg/mL streptomycin] containing IL-4 (20 ng/mL) and GM-CSF (20 ng/ml) (PeproTech) and cultured for 5d with replacement of half of the medium and addition of fresh cytokines every other day ...
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bioRxiv - Neuroscience 2024Quote: ... and IFN-γ (20 ng/mL) (M1 stimulation) or IL-4 (20 ng/mL) and IL-13 (15 ng/mL, all from Peprotech) (M2 stimulation).
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bioRxiv - Developmental Biology 2024Quote: ... 3 μM CHIR treatment was performed from day 4 alongside 50ng/ml Fgf2 (Murine FGF-basic, Peprotech, 450-33-100). CHIR treatment was maintained for two days while Fgf2 was maintained for three additional days.
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bioRxiv - Genetics 2021Quote: ... 10μg/L insulin-like growth factor-1 (IGF-1) (Peprotech, USA), and 1% insulin transferrin selenium (ITS ...
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bioRxiv - Cancer Biology 2022Quote: ... Transforming growth factor beta-1 (TGFb-1) was obtained from Peprotech.
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bioRxiv - Immunology 2021Quote: ... Th17 cell cultures received 5 ng/mL TGF-β and 50 ng/mL IL-6 recombinant and 20 µg/mL anti-IL-4 and 100 µg anti-IFN-γ (PeproTech). After 48 h ...
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bioRxiv - Cell Biology 2021Quote: ... purified and cultured with 10% FBS containing GM-CSF (10 ng/mL) and IL 4 (10 ng/mL) (all PeproTech, USA). FBS (10% ...
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bioRxiv - Biophysics 2021Quote: ... after 24 h recombinant human IL-4 (20 ng/ml) and hSCF (100 ng/ml) (both Peprotech, Rocky Hill, NJ, USA) were added ...
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bioRxiv - Microbiology 2021Quote: ... and fresh 10% fetal bovine serum (10% FBS) with GM-CSF (10 ng/mL) and IL-4 (10 ng/mL) (all PeproTech, USA) were added ...
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bioRxiv - Cell Biology 2021Quote: ... Macrophages were then skewed towards the M2 phenotype using recombinant human IL-4 (20 ng/ml) (Peprotech, Cat#AF-200-04), IL-13 (20 ng/ml ...
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bioRxiv - Immunology 2024Quote: ... Transduced cells were incubated for 4 days with plate-bound anti-CD3/anti-CD28 in media supplemented with hIL-2 (0.0344 units/mL, Peprotech #200-02). Transduction efficiency was assessed for each experiment by flow cytometry.
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bioRxiv - Cancer Biology 2022Quote: ... PBMCs were harvested from K3EDTA anti-coagulated whole blood by density gradient centrifugation with Lymphoprep and plated in 12 well plates at 2-4 × 106 cells per well in 10% RPMI + 10 ng/mL human CSF (300-25, Peprotech, UK). Medium was replaced every 2-3 days until adherent populations of macrophages had established ...
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bioRxiv - Immunology 2024Quote: ... together with 10 ng/mL recombinant mouse IL-4 or 3 μg/mL LPS with 10 ng/mL recombinant murine IFN-ψ (Peprotech) and 20 ng/mL recombinant human BAFF or 5 μg/mL anti-CD40 (Bio X Cell ...
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bioRxiv - Cancer Biology 2023Quote: ... with 100 μl of processed CM in the presence or absence of anti-LIF antibody (4 μg/ml) or recombinant mouse LIF (0.1 μg/ml; Peprotech, 250-02). Spheroids were quantified after 8 d culture.
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bioRxiv - Neuroscience 2022Quote: ... then 4 million cells were seeded per well in 2 ml of EB Medium: complete mTeSR + 0.05 ug/ml BMP-4 (PeproTech, #120-05) + 0.05 ug/ml VEGF (PeproTech ...
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bioRxiv - Neuroscience 2022Quote: ... qNSCs were treated with aNSC media with the addition of 0.5 μg/mL of the BMP-4 antagonist noggin (PeproTech 120-10C) to more reproducibly induce quiescence exit.
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bioRxiv - Immunology 2022Quote: ... Whole mouse splenocytes and human PBMCs were plated at a density of 5×106 cells per well in a Cepallet W-type 24 well microplate (DIC, Japan) and cultured for 4 days with or without mouse/human M-CSF (Peprotech, USA). Surviving macrophages adhered to 24-well plates were detached by lowering the temperature on ice ...
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bioRxiv - Immunology 2024Quote: ... was used for sensitizing the cells in the presence of recombinant human IL-4 (Gibco, PeproTech, #200-04; 10 ng/mL) for three days ...
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bioRxiv - Cell Biology 2024Quote: ... centrifuged and resuspended in satellite cell growth medium (SCGM: F-10 Ham’s medium supplemented with 20% FBS, 4 ng/ ml FGF2 (PeproTech; 100-18B), 1% penicillin–streptomycin and 10mM HEPEs) ...
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bioRxiv - Cell Biology 2024Quote: ... containing growth factors (50 ng ml−1 EGF, PeproTech; 500 ng ml−1 R-spondin, PeproTech; and 100 ng ml−1 Noggin, PeproTech) were added and refreshed every 2 or 3 days ...
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bioRxiv - Neuroscience 2024Quote: ... the expansion medium was gradually exchanged to FGF medium (Neurobasal medium containing 2% B27, 1% NEAA, 1% Glutamax, 1% FBS, and 8 ng/ml human FGF (Peprotech), 5 ng/ml human CNTF (Peprotech ...
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bioRxiv - Bioengineering 2024Quote: ... and 1:100 vol/vol respectively with ACA and inuslin-like growth factor-1 (IGF-1) (PeproTech, 100-11R3-1MG) added to the whole solution at a ratio of 1:50 vol/vol and 1:20000 vol/vol respectively ...
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bioRxiv - Cell Biology 2021Quote: ... 250 ng.mL-1 amphotericin B and 25 ng.mL-1 hM-CSF (PeproTech) for 5 to 7 days ...
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bioRxiv - Immunology 2022Quote: ... mixed 1:1 with serial dilutions of either CXCL4 (Peprotech, Hamburg, Germany) or CXCL4L1 (ChromaTec ...
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bioRxiv - Bioengineering 2021Quote: ... 1 % P/S and 1 ng/ml human FGF2 (Peprotech, 100-18B). When the MSCs reached 80-90 % confluency ...
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bioRxiv - Neuroscience 2021Quote: ... Cells were dissociated using 5mM EDTA in HBSS and resuspended in media with B-27 supplement for 4 hours before incubation with recombinant BDNF protein (100nM, Peprotech, #450-02) or vehicle (equal volume of 0.1% BSA in H2O) ...
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bioRxiv - Immunology 2020Quote: ... cells were washed and cultured for a further 4 days in media containing 20 ng/mL recombinant human IL-2 (Peprotech, London, UK). For analysis of effector cytokine production ...
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bioRxiv - Immunology 2021Quote: ... Cells were cultured at 1×106 cells/ml in complete RPMI-1640 supplemented with 20 ng/ml GM-CSF and 5 ng/ml IL-4 (PeproTech, Cranbury, NJ) for 7 days (Replaced 70% of medium on Day 2 and Day 5) ...
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bioRxiv - Immunology 2020Quote: ... Alternately M2 MΦs were activated as follows: M2a MΦs from stimulation with 20 ng/mL recombinant human IL -4 (PeproTech; Rock Hill, NJ, USA) and 25 ng/mL recombinant human IL-13 (PeproTech ...
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bioRxiv - Immunology 2020Quote: ... and Th17 (anti-IFN-γ-10μg/ml, anti-IL-4–10μg/ml, IL-6-Peprotech,20ng/ml, TGF-β-Peprotech,2ng/ml) polarizing conditions for 4 days ...
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bioRxiv - Immunology 2020Quote: ... and Th17 (anti-IFN-γ-10μg/ml, anti-IL-4–10μg/ml, IL-6-Peprotech,20ng/ml, TGF-β-Peprotech,2ng/ml) polarizing conditions for 4 days ...
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bioRxiv - Cancer Biology 2023Quote: ... cells were cultured in the absence of EGF and FGF and with the addition of either 10 ng/mL BMP-4 (Peprotech; #315-27) or 1% or 2% foetal bovine serum (Gibco ...
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bioRxiv - Immunology 2023Quote: ... in the presence of 40 ng/ml of granulocyte-macrophage colony-stimulating factor (GM-CSF) and IL-4 (both from Peprotech or Biolegend). Cells were cultured in 24-well plates in DMEM (Gibco) ...
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bioRxiv - Systems Biology 2024Quote: ... Osteogenic differentiation was initiated on Day 0 by culturing in osteogenic differentiation medium consisting of growth medium and 100 ng/mL bone morphogenetic protein-4 (BMP4) (PeproTech, 315-27). The osteogenic differentiation medium was replaced every second day.
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bioRxiv - Neuroscience 2024Quote: ... OPCs from each animal were expanded in 3x 175cm2 flasks for 3-4 days in the presence of 10ng/mL platelet derived growth factor-aa (PDGFAA, Peprotech 100-13A). Cells were harvested fresh for each round of experiments and used at the time of first passage ...
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bioRxiv - Molecular Biology 2020Quote: ... TGF-β 1(Peprotech) was added into the media at concentration of 10ng/ml ...
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bioRxiv - Biochemistry 2020Quote: ... 1 μM forskolin (PeproTech), 1 μM Wnt-C59 (Bio-Techne Ltd. ...
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bioRxiv - Cell Biology 2020Quote: ... 1 μM PGE2 (Peprotech), 50% Wnt3a (conditioned medium) ...
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bioRxiv - Neuroscience 2022Quote: ... and IGF-1 (Peprotech), plated onto poly-D-lysine (100µg/ml ...
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bioRxiv - Biophysics 2022Quote: ... EGF (Peprotech,1 mg): Resuspend at 100 ug/ml in sterile ddH2O and store aliquots at -20°C ...
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bioRxiv - Developmental Biology 2023Quote: ... 1 μM PD0325901 (Peprotech) and 3 μM CHIR99021-at 37 °C and 5% CO2 incubating condition ...