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Citations for Peprotech :
251 - 300 of 389 citations for Tryptic Soy Agar TSA CRITERION Dehydrated Culture Media since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2020Quote: ... Neuronal maturation and terminal differentiation were achieved by plating the neural stem cells in the density of 25,000-35,000 cells/cm2 in the Neural Maturation Media (NMM) composed of NBM supplemented with BDNF (20ng/ml) (450-02, Peprotech), GDNF (10ng/ml ...
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bioRxiv - Neuroscience 2020Quote: ... Expansion of the neural progenitor cells was carried out in Neural Expansion Media (NEM) which is composed of NBM supplemented with FGF (10ng/ml) (100-18C, Peprotech) and EGF (10ng/ml ...
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bioRxiv - Neuroscience 2020Quote: Treatment of primary neurons - The primary neurons were treated with APOE from conditioned media or recombinant APOE protein (350-02, 350-04, Peprotech). For conditioned media treatment ...
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bioRxiv - Immunology 2021Quote: ... and the clones with the highest efficacy were expanded and adapted for suspension growth in Pepro AF-CHO serum free media (Peprotech). Six liters of media at 5×106 cells/ml was centrifuged at 1000xg for 30 minutes and the secreted protein was purified to homogeneity as previously described (Albright et al. ...
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bioRxiv - Neuroscience 2021Quote: ... the following inhibitors were added to the NPC media (to make N2:B27-FGF): 10 ng/mL bFGF (basic Fibroblast Growth Factor; Peprotech), 100 μM β-mercaptoethanol (Life Technologies) ...
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bioRxiv - Neuroscience 2020Quote: ... followed by another seven days of neural proliferation media supplemented with 20 ng/mL of epidermal growth factor (EGF, Peprotech). Neuronal Maturation step was achieved by changing the media to Neurobasal with GlutaMAX ...
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bioRxiv - Immunology 2020Quote: ... the non-adherent cells were transferred to a new petri dish and incubated with BMDM media + 20 ng/ml macrophage-colony stimulating factor (M-CSF; Peprotech). After 3 days ...
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bioRxiv - Neuroscience 2020Quote: ... The plate was incubated overnight at 37 °C to allow for SF attachment and the following morning media was replaced with either 200µl of fresh DMEM containing 10 ng/ml of TNF-α (PeproTech), or fresh DMEM alone ...
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bioRxiv - Immunology 2020Quote: ... and were then cultured at 1-2 x 106/ml in 12-well plates with differentiation media containing 50 ng/ml Interleukin 4 (Peprotech) and 100 ng/ml granulocyte-monocyte colony stimulating factor (GM-CSF ...
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bioRxiv - Cell Biology 2020Quote: ... lines were maintained in StemFiT AK02 media (Ajinomoto) supplemented with 100 ng/ml recombinant human basic fibroblast growth factor (bFGF, Peprotech) (hereafter F/A media ...
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bioRxiv - Molecular Biology 2021Quote: ... and 3 x 106 cells were plated to grow in suspension in 90 mm bacterial petri dishes containing N2B27 + Dox media supplemented with 10 ng/mL EGF and FGF (Peprotech). On day 10 ...
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bioRxiv - Immunology 2020Quote: ... approximately 2 x 106 cells were then seeded per 100 mm plate in 10 ml of media containing 20 ng/ml of recombinant murine (rm) GM-CSF (Peprotech). On day 3 ...
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bioRxiv - Immunology 2021Quote: ... 1×106 cells/chamber were subjected to chemotaxis in RPMI media with or without the chemoattractant (human IL-27 (500 ng/ml from PeproTech) or N-formyl-methionyl-leucyl-phenylalanine (fMLP ...
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bioRxiv - Molecular Biology 2022Quote: ... Both SFEM II and our custom expansion media were supplemented with 100 ng/mL stem cell factor (Peprotech, 250-03), 40 ng/mL insulin-like growth factor (Peprotech ...
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bioRxiv - Cancer Biology 2023Quote: ... CD14+ monocytes were cultured for 7 days in Roswell Park Memorial Institute media containing 10-ng/mL human granulocyte-macrophage colony-stimulating factor and 10-ng/mL human IL-6 (PeproTech). For cell binding competition with AL009 ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells were passed through 70-micron cell strainer and subsequently cultured 10mL complete RPMI media supplemented with 20 ng/mL of recombinant murine M-CSF (576406; BioLegend, 315-02 PeproTech). On day 3 ...
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bioRxiv - Neuroscience 2023Quote: ... 10% of the total volume from the iMG conditioned media was added to fresh N2B27 with 10ng/ml BDNF (450-02, Peprotech) and 10ng/ml GDNF (450-10 ...
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bioRxiv - Immunology 2023Quote: ... and cultured in complete RPMI 1640 media (10% fetal bovine serum, 1% penicillin-streptomycin, 1% L-glutamine) supplemented with 10 ng/mL M-CSF (PeproTech). Cells were cultured for 5 days at 37°C in a humidified 5% CO2 atmosphere under continuous high-dose 100 ng/mL lipopolysaccharide (LPS ...
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bioRxiv - Bioengineering 2023Quote: ... At Day 11 the cytokine composition of the media was changed to 10ng/μl of bFGF and human recombinant Hepatocyte Growth Factor (HGF) at 10ng/μl (Peprotech). From Day 15 onwards ...
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bioRxiv - Immunology 2023Quote: Primary Keratinocytes from three donors were seeded in 24-well plates and stimulated with KGM Media supplemented either with 0.1 ng/ml or 10 ng/ml of recombinant human IFN-γ (PeproTech), or infected with T ...
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bioRxiv - Molecular Biology 2022Quote: ... MA) or sterile PBS containing 0.01% Tween-20 immediately followed by treatment media with or without TNFα (Peprotech, Cranbury, NJ) for 15 min ...
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bioRxiv - Immunology 2022Quote: Single bone marrow cell suspensions were plated at 4×106 per well in 2 mL of complete IMDM media supplemented with 150 ng/mL of FLT3L (PeproTech). On day 7 of differentiation ...
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bioRxiv - Developmental Biology 2024Quote: ... expanded neural progenitors were re-plated at a density of 3×104 cells/cm2 in expansion media and 24 hours later switched to N2B27 supplemented with 10 ng/mL human recombinant CNTF (Peprotech) and 10 ng/mL human recombinant BMP4 (Peprotech ...
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bioRxiv - Developmental Biology 2024Quote: ... cells were replated as single cells onto poly-D-lysine-laminin-coated plates at 1×106 cells/cm2 for astrogenic switch and progenitor expansion in N2B27 media supplemented with 10 ng/mL FGF2 (Peprotech) and 10 ng/mL Human EGF (Peprotech ...
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bioRxiv - Genomics 2023Quote: ... and 3 x 106 cells were plated to grow in suspension in 90 mm bacterial petri dishes containing N2B27 media supplemented with 1 μg/ml doxycycline and 10 ng/ml EGF and FGF (Peprotech). Embryoid-body-like aggregates were collected by mild centrifugation (100 g for 2 min ...
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bioRxiv - Neuroscience 2023Quote: ... Media was changed every 24h up to day 10 on which cells were mechanically clump passaged using 0.02% EDTA in PBS onto fibronectin coated plates and maintained in the same media without SMAD inhibitors and including 25ng/ml activin-A (Peprotech). All subsequent media changes were half volumes and every 48h before passaging again onto poly-l-ornithine and laminin coated 12w plates on day 20 ...
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bioRxiv - Cancer Biology 2023Quote: ... These tumoroids were then cultured in BEN selection media (basal medium with mouse recombinant EGF (50 ng/ml) and Noggin (100 ng/ml) (PeproTech) without R-spondin ...
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bioRxiv - Biochemistry 2023Quote: ... cells were plated (60,000 cells/well in 24-well plates) two days before treatment in complete media supplemented with 25 ng/mL murine IL-4 (Peprotech) for M2-polarization or without any cytokines (M0) ...
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bioRxiv - Cancer Biology 2023Quote: ... Differentiation of BM cells into DCs was carried out in low attachment 10 mm cell culture dish in presence of bone marrow-differentiation media in presence of recombinant murine Granulocyte-Macrophage Colony-Stimulating Factor (GM-CSF) (Cat. 315-03, Peprotech) for 48 a ...
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bioRxiv - Genetics 2023Quote: ... An aliquot of 500,000 cells were plated in 200 µL of media in a 96-well plate and stimulated with 20 ng/mL of IL-6 (Peprotech) for 15 minutes ...
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bioRxiv - Genomics 2023Quote: ... CD34+ HSPCs were differentiated to the megakaryocyte lineage by culturing for 11 days in IMDM based media containing 30ng/mL rhTPO (PeproTech), 1ng/mL rhSCF (PeproTech) ...
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bioRxiv - Immunology 2023Quote: ... beads taken off each well and replated for resting in T cell media containing 10 U/mL of Interleukin-2 (IL-2; Peprotech). To restart each stimulation cycle ...
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bioRxiv - Immunology 2022Quote: ... The lower compartments were filled with 600 µL of complete IMDM media with or without 200 ng/mL of CCL19 (PeproTech). After incubation at 37°C for 21 hours ...
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bioRxiv - Cancer Biology 2022Quote: ... Quiescence was induced by plating cells at a density of 10 cells/mm2 in NSC media in the absence of EGF/FGF-2 and supplemented with BMP4 (10 ng/ml, Peprotech). Cells were treated for 1 day or 3 days ...
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bioRxiv - Cell Biology 2022Quote: ... The monolayer was washed 3 times with HBSS to remove non-adherent cells and monocytes were differentiated into macrophages for 3 days in RPMI media + 10% FBS containing 30 ng/ml M-CSF (PeproTech) and for further 4 days in media containing 30 ng/ml RANKL (PeproTech ...
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bioRxiv - Immunology 2023Quote: ... The transfection mix was then added dropwise to 2.5×106 M0 macrophages (pre-cultured for 6 days in a 6-well plate in antibiotic-free RPMI1640 macrophage media containing M-CSF (50ng/ml, Peprotech) – with media change on d3) ...
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bioRxiv - Genetics 2023Quote: ... and seeded on a poly-D-lysine/laminin plate with N2B27 media supplemented with BDNF and GDNF (10 ng/ml each; Peprotech). Media were changed the next day to facilitate neuronal maturation and survival ...
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bioRxiv - Developmental Biology 2024Quote: ... On day 11 the cytokine composition of the media was changed to 10ng/ml of bFGF and human recombinant Hepatocyte Growth Factor (HGF) at 10ng/ml (Peprotech). From day 15 onwards ...
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bioRxiv - Cancer Biology 2024Quote: ... HSCs were transferred into 200 µl of complete HSC media supplemented with 100ng/ml recombinant mouse TPO and 10ng/ml recombinant mouse SCF (PeproTech Recombinant Murine TPO Catalogue Number ...
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bioRxiv - Cell Biology 2024Quote: ... for 3 days and then cultured in 10% FBS α-MEM media supplemented with 100ng/mL RANKL (PeproTech, 315-11) and 30 ng/mL M-CSF for 6 to 7 days ...
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bioRxiv - Molecular Biology 2024Quote: ... Neuronal maturation and terminal differentiation were achieved by plating the NSCs at a density of 25,000-35,000 cells/ cm2 in the Neural Maturation Media (NMM) composed of NBM supplemented with BDNF (20 ng/ml) (450-02, Peprotech), GDNF (10 ng/ml ...
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bioRxiv - Immunology 2024Quote: ... Cells were then resuspended in RPMI 1640 media containing 30 ng/ml GM-CSF and 20 ng/ml recombinant human TGF-β1 (PeproTech) and plated in untreated 48- or 24-well plates ...
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bioRxiv - Cancer Biology 2024Quote: ... Media was supplemented with VEGF (50 ng/mL) on all days and Angiopoietin 1 (Ang-1) (100 ng/mL) (Peprotech) from day 3 onward.
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bioRxiv - Neuroscience 2024Quote: ... CD11b and CD45 were derived from mesodermally-patterned embryoid bodies cultured in myeloid precursor media containing 100 ng/ml M-CSF (Peprotech) and 25 ng/ml IL-3 (Gibco ...
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bioRxiv - Immunology 2024Quote: ... PBMCs were thawed at 37°C and incubated in complete RPMI media with 10% heat-inactivated FBS and supplemented with IL-2 (50 U/ml; PeproTech). 1 x 104 PBMCs were plated per well in round bottom clear plate (Greiner ...
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bioRxiv - Immunology 2024Quote: ... Samples were stimulated and incubated overnight in complete RPMI media with 10% heat-inactivated FBS and supplemented with IL-2 (50 U/ml; PeproTech).
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bioRxiv - Immunology 2024Quote: ... KHYG1 cells were then single-cell sorted into 96-well plates and grown in RPMI-1640 media supplemented with recombinant human IL-2 1000 U/mL (Peprotech) for 5-6 weeks ...
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bioRxiv - Molecular Biology 2024Quote: ... The NSCs were maintained in Neural Expansion Media (NEM) composed of NBM supplemented with FGF (10 ng/ml) (100-18C, Peprotech) and EGF (10 ng/ml ...
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bioRxiv - Cancer Biology 2024Quote: ... cells were detached with 0.1 mM EDTA (Life Sciences) and re-plated with media supplemented with 10 μM Y-27632 Dihydrochloride (PeproTech, #1293823). CHLA-01-Med primary and CHLA-01R-Med recurrent MB cell lines purchased from ATCC were cultured in suspension in Gibco DMEM-F12 media (Life Technologies ...
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bioRxiv - Bioengineering 2024Quote: ... media was changed every other day with M2 + 20 ng/mL FGF2 + 20 ng/mL EGF (PeproTech, AF-100-15). From days 26-29 ...