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Citations for Peprotech :
251 - 300 of 327 citations for Tripartite Motif Containing Protein 55 TRIM55 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... and polyclonal biotinylated rabbit anti-RELMα detection antibodies (all Peprotech) were used according to a previously described protocol [38].
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bioRxiv - Cancer Biology 2022Quote: ... Cells were afterwards stimulated with indicated amount of recombinant protein CXCL2 (250-15-20, PeproTech GmbH) in DMEM medium without FCS or conditioned medium of endothelial cells.
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bioRxiv - Cancer Biology 2023Quote: ... or c) supplemented with recombinant human IGFBP5 protein (500 ng/ml; Peprotech, Rocky Hill, NJ, USA). Cell lysates were obtained using RIPA buffer with the Halt Protease and Phosphatase Inhibitor Cocktail (Thermo Fisher ...
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bioRxiv - Cell Biology 2021Quote: ... purified and cultured with 10% FBS containing GM-CSF (10 ng/mL) and IL 4 (10 ng/mL) (all PeproTech, USA). FBS (10% ...
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bioRxiv - Immunology 2020Quote: ... containing 9 µg of encapsulated GM-CSF (Murine GM-CSF, Sanofi, USA; Human - Leukine® for pig and rabbit, Peprotech, USA) were combined with a 1 mL solution of captured antigen ...
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bioRxiv - Cell Biology 2020Quote: ... The monolayer was washed 3 times with HBSS to remove non-adherent cells and monocytes were differentiated for 3 days in RPMI media containing 20 ng/ml M-CSF and 20ng/ml recombinant human RANKL (PeproTech, UK). After 3 days of culture ...
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bioRxiv - Developmental Biology 2019Quote: ... Cells were then washed 2 times in PBS and replaced with medium containing fresh SB431542 or 5ng/mL TGFβ1 (Peprotech, 100-21C) or 10ng/mL Activin A (Sigma Aldrich ...
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bioRxiv - Biochemistry 2019Quote: ... The cells were subject to cytokines stimulation in serum free medium containing 10 ng/ml rat IFN-γ (Peprotech, #400-20) and 10 ng/ml rat TNF-α (Peprotech ...
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bioRxiv - Developmental Biology 2023Quote: ... and were maintained under a feeder-free condition in the StemFit® Basic03 medium (SFB-503, Ajinomoto) containing bFGF (100-18B, Peprotech). Prior to passaging ...
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bioRxiv - Developmental Biology 2023Quote: ... the media was replaced with Xen medium containing 0.1 µM retinoic acid (Thermo Fischer, #17110052) and 10 ng/mL Activin A (Peprotech, #120-14). On Day4 ...
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bioRxiv - Immunology 2024Quote: ... A single round of co-sedimentation with retroviral stock was performed in medium containing 5% WEHI-3B-conditioned medium and 10 ng/mL IL-7 (Peprotech, USA). After transduction ...
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bioRxiv - Microbiology 2020Quote: ... Frozen activated PBMC cells were thawed and resuspended in complete PBMC media containing RPMI supplemented with 21 ng/mL human IL-2 (Peprotech; #200-02), 10% fetal bovine serum and 1% penicillin-streptomycin ...
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bioRxiv - Microbiology 2020Quote: ... Pellets were resuspended in activation medium containing RPMI supplemented with 21 ng/mL human IL-2 (Peprotech, Rocky Hill, NJ; #200-02), 1.5 μg/mL PHA (Remel ...
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bioRxiv - Cell Biology 2021Quote: ... bone marrow progenitor cells were collected in cRPMI and replated in cRPMI containing 20 ng/mL recombinant murine GM-CSF (Peprotech, 315-03) (DC media) ...
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bioRxiv - Immunology 2022Quote: ... Cells were transdifferentiation for 7 days at 60,000 cells/cm2 in medium containing 50 ng/ml IL-3 (200-03 B, Peprotech, New Jersey, USA), 50 ng/ml M-CSF (300-25 B ...
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bioRxiv - Cancer Biology 2022Quote: Single-cell suspensions of cell lines were suspended at a density of 2,000 cells/ml in DMEM/Ham’s F-12 (nacalai tesque, no. 11581-15) containing 20 ng/ml bFGF (Peprotech, no. 100-18B), 20 ng/ml EGF (Wako ...
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bioRxiv - Immunology 2020Quote: ... cells were washed and cultured for a further 4 days in media containing 20 ng/mL recombinant human IL-2 (Peprotech, London, UK). For analysis of effector cytokine production ...
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bioRxiv - Immunology 2022Quote: ... primary BMDMs were grown for 7 days in complete RPMI media containing 20 ng/mL of recombinant MCSF (Peprotech, Rocky Hill, NJ) prior to use in assays ...
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bioRxiv - Biochemistry 2022Quote: ... cells were diluted to a density of 6e4/mL and 50 μL (3e3 cells) were added to 50 μL of media containing 2x of a serial dilution of human CSF3 (PeproTech 300-23) in a 96-well plate ...
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bioRxiv - Immunology 2022Quote: A single cell suspension was prepared by flushing the bone marrow from the hind legs in RPMI containing 10% FCS buffer with 10 ng/mL IL6 (Peprotech, 216-16). A red blood cell lysis step was performed in 2 mL ammonium-chloride-potassium (ACK ...
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bioRxiv - Immunology 2023Quote: ... Bone marrow was plated into non-cell-culture treated 10cm petri dishes in 8mL supplemented RPMI medium containing either 20ng/mL recombinant M-CSF (Peprotech, #315-02) or 20ng/mL recombinant GM-CSF (Peprotech ...
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bioRxiv - Cancer Biology 2023Quote: ... unsorted bone marrow cells from Myc+/+ or MycT58A/T58Amice (25,000 cells/ml) were cultured in IMDM containing stem cell factor (SCF Peprotech, 50 ng/ml), IL-6 (Peprotech ...
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bioRxiv - Bioengineering 2024Quote: ... Cells were then moved to a flat-bottom 96 well plate containing 160 uL of medium supplemented with 500 U/mL IL-2 (Peprotech, Cranbury, NJ) or 10 ng/mL of IL-7 (BioTechne ...
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bioRxiv - Cell Biology 2023Quote: ... BMMs were seeded at a density of 1.2 × 105 cells/well into 24-well culture plates and incubated in α-MEM (SH30265.01; Hyclone, Rockford, IL, USA) containing 20 ng/mL macrophage colony-stimulating factor (M-CSF) (300-25; PeproTech, Cranbury, NJ, USA). To induce osteoclast differentiation ...
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bioRxiv - Cell Biology 2020Quote: ... the organoids were grown in DM supplemented with bone morphogenetic protein 4 (BMP4) (50 ng/mL, PeproTech) or Purmorphamine (1 μm ...
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bioRxiv - Cell Biology 2019Quote: ... and bone morphogenetic protein-2 (BMP2) 300 ng/ml (Peprotech, cat. # 120-02-2UG, Rocky Hill, NJ). The SSC used for differentiation experiments were no older than passage 3 in culture.
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bioRxiv - Cancer Biology 2023Quote: ... Supernatants were assayed for IL-12 protein with the Murine IL-12 Standard ELISA Development Kit (Peprotech) according to manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... then incubated with anti-RELMα primary antibody (Peprotech, Cat# 500-P214), and subsequently an anti-rabbit secondary antibody (Life technologies) ...
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bioRxiv - Immunology 2020Quote: ... Chemokine oligomers were detected by specific rabbit anti-chemokine antibodies (Peprotech) followed by development with an HRP-conjugated anti-rabbit antibody (Abcam).
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bioRxiv - Cancer Biology 2021Quote: ... The primary antibodies used were: anti-PTHrP (Peprotech, Cat# 500-P276), anti-β-casein (Santa Cruz Cat# 166530) ...
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bioRxiv - Cell Biology 2023Quote: ... and TGFβ neutralizing antibodies were purchased from PeproTech (Rocky Hill, NJ).
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bioRxiv - Molecular Biology 2019Quote: ... 10 weeks old) were subcutaneously injected into the groin regions with 400 μL Matrigel containing recombinant mouse basic FGF (PeproTech, 250 ng/mL) and heparin (Sigma ...
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bioRxiv - Cell Biology 2021Quote: ... the transwells were dipped twice in PBS and transferred to a 6-well plate containing NSC medium (DMEM:F12 – glutamax, 1% pen/strep, 10 ng/mL EGF (Peprotech, AF-100-15-A), 10 ng/mL FGF (Peptrotech ...
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bioRxiv - Immunology 2019Quote: ... the cells were resuspended and cultured in macrophage-conditioned medium containing complete 1640 medium containing 10% (vol/vol) FBS and 100 U/ml penicillin and streptomycin and 20 ng/ml murine M-CSF (Peprotech, cat. # 315-02) for 7 days.
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bioRxiv - Immunology 2022Quote: ... and histone isolation procedures activated CD8+ Teff cells were re-cultured (5 × 106 cells/well in 6-well plates) for up to 48h in IMDM containing 50 U/mL IL-2 (PeproTech, Rocky Hill, NJ) prior to use in assays ...
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bioRxiv - Immunology 2022Quote: ... Cells were differentiated to macrophages in RPMI x1640 supplemented with 10% FBS containing 10 ng/ml of recombinant human GM-CSF (Peprotech, New Jersey, US) for 7 days ...
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bioRxiv - Cell Biology 2023Quote: ... neuromuscular co-cultured were gently covered with 2 mL of DM containing brain-derived neurotrophic factor (BDNF, 10 ng/mL, PeproTech 450-02-10μg) and glial cell-derived neurotrophic factor (GDNF ...
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bioRxiv - Bioengineering 2024Quote: ... NK cells were allowed to recover at a density of 1E6 cells/mL in antibiotic-free medium containing 100 IU/mL IL-2 (PeproTech, Rocky Hill, NJ) for 72 hours at 37°C ...
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bioRxiv - Developmental Biology 2024Quote: ... proteins were supplemented in Maturation media by dissolving Matrigel at 1% (v/v) containing human recombinant brain-derived neurotrophic factor (BDNF; PeproTech, AF-450-02). The aggregated cells were referred to as cerebral organoids from this stage onward and were allowed to further develop in maturation media until the experimental endpoints ...
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bioRxiv - Neuroscience 2022Quote: ... IL-34 (100 ng/mL) and GM-CSF (10 ng/mL) (all protein growth factors were from Peprotech). Prior to thaw ...
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bioRxiv - Bioengineering 2023Quote: ... then for the next 4 days media was supplemented with 50ng/mL bone morphogenetic protein 4 (BMP4) (Peprotech), and for the final 4 days 50ng/mL of hepatocyte growth factor (HGF ...
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bioRxiv - Immunology 2021Quote: ... antibody alone or in combination with IL-4 (20 ng/ml, Peprotech) for up to 1 day ...
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bioRxiv - Biophysics 2023Quote: ... we tuned the cellular contractility by replacing the medium with a new one containing recombinant human TGF-β (2.5 ng/mL, 100-21, PeproTech, Rocky Hill, NJ, USA), to promote cellular contractility ...
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bioRxiv - Cell Biology 2023Quote: ... cells were plated in 1% FBS- containing DMEM for 72 hrs with or without 4ng/mL human recombinant TGF-β (Peprotech; cat #100-21).
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bioRxiv - Neuroscience 2021Quote: ... Cells were treated with either vehicle (equal volume added of 0.1% BSA in H2O) or 100nM BDNF recombinant protein (Peprotech, #450-02 ...
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bioRxiv - Neuroscience 2020Quote: ... the membrane was incubated with anti-mCSF1 antibody (1:1000, PeproTech, 500-P62G) at 4°C overnight ...
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bioRxiv - Immunology 2021Quote: ... All cytokines and antibodies noted above were purchased from PeproTech (Rocky Hill, NJ) and BioXCell (West Lebanon ...
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bioRxiv - Immunology 2022Quote: ... All the cells were labeled with monoclonal anti-human TNFα antibody (Peprotech, USA) and subsequently washed with 1X PBS followed by treatment with FITC-conjugated secondary anti-mouse antibody ...
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bioRxiv - Molecular Biology 2019Quote: ... After 7 days cells were detached using TryplE and seeded on non-gelatinized bacterial dishes for suspension culture at 3 million cells per 75cm2 and cultured in N2B27 containing 10ng/mL EGF and FGF (Peprotech 315-09 and 100-18B). After 3 days floating aggregates were seeded on gelatinized dishes ...
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bioRxiv - Immunology 2021Quote: ... Sorted cells were seeded in 10 cm tissue-culture dishes of 80 – 90% confluent OP9 cells containing 5 ng/ml Flt-3L (R&D, Catalogue no.-427-FL) and 1 ng/ml IL-7 (PeproTech, Catalogue no.- 217-17-10). For T-cell development assay ...