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Citations for Peprotech :
2351 - 2400 of 2402 citations for IL 1RL2 Human HEK293 Fc since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: ... then 50 µL of 24 h co-culture supernatants or serial dilutions of Recombinant Human IFNγ standard (#300-02, Peprotech, USA) were added to the wells and incubated for 2 h at RT ...
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bioRxiv - Developmental Biology 2023Quote: ... The differentiation medium was composed of modified N2B27 medium supplemented with 50 ng/ml recombinant human FGF4 (Peprotech, 100-31-25), 1 μg/ml heparin (Sigma ...
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bioRxiv - Immunology 2024Quote: ... NBP2-35892), IFNω (Novusbio, NBP2-35893), IFNγ (Novusbio, NBP2-34992), IFNλ1 (Novusbio, NBP2-34996) and recombinant human TNFα (PeproTech, 300-01A).
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bioRxiv - Immunology 2024Quote: ... 50 μM 2-mercaptoethanol) with 100 ng/ml of human FMS-like tyrosine kinase 3 ligand (FLT3L, PeproTech, Cranbury, NJ, USA) for 7 days ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were deprived of serum for 2h and stimulated with 2 ng/ml of recombinant human EGF (Peprotech; Catalog #AF-100-15) for 2 ...
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bioRxiv - Bioengineering 2021Quote: ... Media was serum-free TexMACS (Miltentyi Biotech 170-076-307) supplemented with recombinant human IL2 in concentrations specified by the DOE (Peprotech 200-02). Cell cultures were expanded for 14 days as counted from the time of initial seeding and activation ...
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bioRxiv - Cancer Biology 2019Quote: ... in 200µl of RPMI supplemented with 2% FBS and 5% Matrigel with or without the addition of human IFN-γ (PeproTech; #300-02), mouse IFN-γ (PeproTech ...
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bioRxiv - Bioengineering 2021Quote: ... HepatoSTIM™ medium was supplemented with 10 ng mL−1 of recombinant human TGF-β1 (100-21; PeproTech®, Rocky Hill, NJ), 100 ng mL−1 of recombinant human GDF-15 (120-28C ...
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bioRxiv - Cancer Biology 2022Quote: ... the selected monocytes were cultured in complete Advanced-RMPI 1640 supplemented with either 20 ng/mL human GM-CSF (PeproTech, RockyHill, NJ), for M1 differentiation ...
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bioRxiv - Immunology 2022Quote: ... OT-I T cells were activated with 10-8 SIINFEKL or SIITFEKL (Cambridge Peptides) in the presence or absence of recombinant human TGFβ (Peprotech, 5ng/ml). Where indicated ...
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bioRxiv - Cancer Biology 2020Quote: ... H1975 cells were cultured in stem cell culture media (Advanced DMEM/F12 [Thermo Fisher Scientific], 1% Glutamax, B-27 supplement [Thermo Fisher Scientific], 10 ng/mL recombinant human HB-EGF [PeproTech, Cranbury ...
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bioRxiv - Pathology 2020Quote: ... HUVEC were plated at a density of 10,000 cells/96-well plate in EGM-2 supplemented with recombinant human TNF-α (300-01A, PeproTech EC Ltd), IL1-β (200-01B ...
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bioRxiv - Developmental Biology 2021Quote: ... LCDMYI supplementation was added as indicated at the following concentrations: 10 ng ml−1 recombinant human LIF (L, 10 ng ml−1; Peprotech, 300-05), CHIR99021 (C ...
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bioRxiv - Biochemistry 2022Quote: ... cells were diluted to a density of 6e4/mL and 50 μL (3e3 cells) were added to 50 μL of media containing 2x of a serial dilution of human CSF3 (PeproTech 300-23) in a 96-well plate ...
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bioRxiv - Bioengineering 2022Quote: ... Hydrogels were cultured in 48 well plates for 7 days and maintained in ECM with or without additional growth factors (± 100 ng/mL recombinant human VEGF165; PeproTech 100-20) and hormones ...
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bioRxiv - Neuroscience 2022Quote: ... hNPCs were plated on poly-L-ornithine- and laminin-coated plates and were differentiated with growth media (without FGF-b) supplemented with 25ng/mL recombinant human glial-derived neurotrophic factor (GDNF; PeproTech US, NJ). Fifty percent of cell media were removed every three to four days and replaced with fresh GDNF supplemented media ...
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bioRxiv - Immunology 2022Quote: ... Specificity was validated by performing normalized supernatant ELISAs against the extracellular domain of human TNFR2 (Peprotech, 310-12 and in-house production). Flow cytometry phenotyping of hybridoma cells was performed on a BD FACS Aria III ...
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bioRxiv - Neuroscience 2024Quote: ... Acute treatment was evaluated in 9 month-old mice by pre-mixing and co-administering 5 µg HCT-555 with 25 ng recombinant human BDNF (Peprotech, 450-02) into one tibialis anterior muscle and 5 µg HCT-555 with PBS vehicle into the contralateral tibialis anterior ...
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bioRxiv - Immunology 2024Quote: HIOs cultured for 7-12 days were treated with 0.1-10 µM indicated JAK inhibitor 1h prior to 50ng/ml human recombinant IFN-γ (300-02, PeproTech, Rehovot, Israel) stimuli for 24h ...
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bioRxiv - Cancer Biology 2024Quote: ... After inhibitor treatment spheroids were treated with a combination of growth factors or vehicle for 30 minutes before fixation in-situ: 100 nM Human IGF1 (Peprotech #100-11) and 100 nM Human EGF (Peprotech #AF-100-15-1mg) ...
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bioRxiv - Immunology 2023Quote: All cell lines were seeded in flat 96-well plates at a density of 0.25 × 105 cells/well and cultured for 24 h in the absence or presence of human recombinant IFNγ and TNFα with activities of 2 × 107 Units/mg (PeproTech, Rehovot, Israel). Cells lines were then co-cultured with 100,000 (at density of 1×106 cell/ml ...
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bioRxiv - Cell Biology 2023Quote: ... The medium was changed every day until day 8 when medium was replaced by E6 medium supplemented with 10 ng/mL human Platelet-Derived Growth Factor BB (PDGF-BB, PeproTech, 100-14B) and 2 ng/mL Recombinant Human Transforming Growth Factor-β1 (TGF-β1 ...
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bioRxiv - Bioengineering 2023Quote: ... M1-like MØs were obtained by culturing M0 MØs in RPMI media supplemented with 10pg/ml Lipopolysaccharides (MilliporeSigma, MO, USA) and 1 ng/ml human Interferon-gamma (Peprotech, NJ, USA). Protocol for generating M0 ...
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bioRxiv - Cancer Biology 2024Quote: ... Exploration of BMP2 effect on HSJD-DIPG-012 and HSJD-DIPG-014 spheroids was done using 10 ng/mL of human recombinant BMP2 (Peprotech, 120-02C) before protein quantification by western blot.
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bioRxiv - Biochemistry 2024Quote: ... Sigma-Aldrich P6407) in OPC proliferation media (DMEM-Sato) supplemented with 10 ng/ml recombinant human ciliary neurotropic factor (CNTF; Peprotech 450-13), 10 µM forskolin (Sigma-Aldrich F6886) ...
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Nanoparticle-delivered TLR4 and RIG-I agonists enhance immune response to SARS-CoV-2 subunit vaccinebioRxiv - Immunology 2022Quote: ... RBC-lysed murine bone marrow was seeded at 2 million cells/mL in complete RPMI media with 200 ng/mL human FLT3 ligand (PeproTech, Cat# 300-19) in a 6-well plate (10 million cells/well) ...
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bioRxiv - Immunology 2022Quote: ... The CD34 cell purity was checked by FACS and cells were cultured in IMDM with 10% HI FBS supplemented with human cytokine and growth factor cocktail from Peprotech (hTPO (#300-18) (10 ng/ml) ...
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bioRxiv - Bioengineering 2021Quote: ... supplemented with 50 ng/mL FGF2 and 30 ng/mL animal-free recombinant human Bone Morphogenic Protein 4 (BMP4, Peprotech, AF-120-05ET) for 24 hours ...
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bioRxiv - Cancer Biology 2021Quote: ... The cells were incubated in RPMI1640 for THP-1 culture supplemented with human M-CSF at 20 ng/ml (cross-react with mouse, Peprotech, Cranbury, NJ, USA). After 2 days of culture ...
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bioRxiv - Microbiology 2021Quote: ... control and CSP-stable transfected HepG2 cells were pre-treated with 1 U/mL recombinant human IFN-γ (Peprotech, Rocky Hill, NJ, USA) or equivoluminal medium for 6 h ...
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bioRxiv - Immunology 2020Quote: ... 1640 Medium supplemented with 10% fetal bovine serum (35-076-CV; Coring, New York, USA) and 20 ng/μL Recombinant Human GM-CSF (300-03; Peprotech, Rocky Hill, USA).BEAS-2B cells (CRL-9609 ...
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bioRxiv - Cancer Biology 2021Quote: ... MCF7 cells were cultured in 2D in the absence or presence of 10 ng/mL human TGFβ (PeproTech®, Rocky Hill, NJ, USA) for 3 weeks where TGFβ was replenished every 2 days ...
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bioRxiv - Immunology 2021Quote: ... Human monocyte-derived macrophages (HMDMs) were differentiated from purified CD14+ monocytes in the presence of recombinant human M-CSF (50 ng/ml; PeproTech, Cranbury, NJ, USA) for 6 days in RPMI 1640 medium supplemented with 10% FBS ...
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bioRxiv - Immunology 2020Quote: ... Isolated PBMC were washed with PBS and resuspended by RPMI-1640 which supplemented 50ng/ml recombinant human macrophage colony-stimulating factor (M-CSF) (AF-300-25-500, PeproTech, Cranbury, NJ, USA), then cultured at 37°C in 5% CO2 for 5 days to differentiate into human monocyte derived macrophages (hMDMs ...
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bioRxiv - Immunology 2022Quote: ... Cells were differentiated to macrophages in RPMI x1640 supplemented with 10% FBS containing 10 ng/ml of recombinant human GM-CSF (Peprotech, New Jersey, US) for 7 days ...
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bioRxiv - Bioengineering 2022Quote: ... MDTs were exposed to a neutral culture medium or to a 20 ng/ml of TNF solution (Recombinant TNF alpha human; 300-01A, PeproTech, Thermo Fisher Scientific) in culture medium for either 30 minutes or 240 minutes ...
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bioRxiv - Developmental Biology 2024Quote: ... proteins were supplemented in Maturation media by dissolving Matrigel at 1% (v/v) containing human recombinant brain-derived neurotrophic factor (BDNF; PeproTech, AF-450-02). The aggregated cells were referred to as cerebral organoids from this stage onward and were allowed to further develop in maturation media until the experimental endpoints ...
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bioRxiv - Immunology 2023Quote: ... were differentiated from PBMCs by culturing the cells in RPMI complete medium supplemented with 10% AB-positive human serum and 15 ng/ml M-CSF (PeproTech, 300-25-100UG). After 2 days of culturing ...
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bioRxiv - Immunology 2023Quote: ... iPSCs were differentiated to macrophages[73] and stimulated for 6h and 24h with 10 ng/mL recombinant human interleukin-10 (Peprotech, 200-10-2), 10 ng/mL recombinant human interferon-b (Peprotech ...
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bioRxiv - Bioengineering 2021Quote: PTMs were differentiated from hiPSCs using the above protocol for hiPSC-derived Leydig cells with the modification that PDGF-AA is replaced by human recombinant Platelet-Derived Growth Factor-BB (PDGF-BB, Peprotech, 100-14B, Figure 1B). On day 25 the cells were expanded on PLL-coated plates in DMEM/F12 ...
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bioRxiv - Neuroscience 2020Quote: ... and 1% insulin transferrin selenium (Cat N° 41400045) in IMDM (Cat N° 12440053) supplemented with 5 ng/ml recombinant human basic fibroblast growth factor (bFGF) (Peprotech, Cat N° 100-18B). Medium was changed every 4 days ...
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bioRxiv - Immunology 2022Quote: ... hCD34+ cells were either kept in culture at 37°C in RPMI medium with human recombinant Stem Cell Factor (SCF) at 50 ng/ml (PeproTech, Rocky Hill, NJ, USA), human recombinant thrombopoietin (TPO ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Differentiated sensory neurons were maintained in N2 medium with neuronal growth factors (10 ng/ml human β-NGF, BDNF, NT3 and GDNF; PeproTech, Rocky Hill, NJ) in a 37°C incubator under 5% CO2 ...
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bioRxiv - Microbiology 2021Quote: ... 2.5×105 HUVECs were plated in wells of 6-well plates and stimulated overnight with 20 ng.ml−1 human recombinant TNFα (PeproTech/Tebu Bio, #300-01A-A) or infected at MOI=200 with Neisseria meningitidis for 30 min ...
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bioRxiv - Neuroscience 2022Quote: ... and plated on low-attachment 6-well plate in E8 with 20 ng/ml human basic fibroblast growth factor (bFGF2; Peprotech, Somerset County, NJ, USA) and 20 µM Rho kinase inhibitor (Sigma-Aldrich ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Immature iPSC-SNs were maintained in N2 medium supplemented with human growth factors (25 ng/mL NGF-β, 450-01; BDNF, 450-02; GDNF, 450-10; NT-3, 450-03; Peprotech, Cranbury, NJ, USA), and 0.2 mM L-ascorbic acid (A4403 ...
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bioRxiv - Biophysics 2023Quote: ... we tuned the cellular contractility by replacing the medium with a new one containing recombinant human TGF-β (2.5 ng/mL, 100-21, PeproTech, Rocky Hill, NJ, USA), to promote cellular contractility ...
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bioRxiv - Cell Biology 2023Quote: ... cells were plated in 1% FBS- containing DMEM for 72 hrs with or without 4ng/mL human recombinant TGF-β (Peprotech; cat #100-21).
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bioRxiv - Cancer Biology 2023Quote: ... the FGF and EGF mitogens in the media were replaced by 100 ng/ml of BMP4 (Recombinant human BMP-4, PeproTech, Cat# AF-120-05ET) (quiescence media) ...
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bioRxiv - Cell Biology 2024Quote: ... Catalog No.501362646), 10 μM SB (SB431542, Selleckchem, Catalog No.101762-616) and 5 ng/mL FGF2 (FGF basic 154aa, human, Peprotech, Catalog No.10771-938).