Labshake search
Citations for Peprotech :
2001 - 2050 of 2430 citations for IL 13 Human HEK 293 mFc since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: Splenic T-cells from Ldlr-/- and T-AbcdkoLdlr-/- mice were isolated and stimulated with Mouse T-Activator CD3/CD28 Dynabeads (ratio beads to cells 1:2) in the presence of 100 U/mL IL-2 (Peprotech) and 5 ng/mL TGFβ1 (Peprotech ...
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bioRxiv - Cancer Biology 2019Quote: ... and maintained in culture for a total of approximately 2-3 months in DMEM supplemented with 1 ng/mL IL-3 (Peprotech). Mycoplasma testing was not performed ...
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bioRxiv - Immunology 2021Quote: 750 000 MCs were either FACS-sorted after 24h of coculture with resting or anti-CD3/28 coated beads-activated effector/memory CD4+ T cells or stimulated for 4 hours with 10 ng/mL of IL-33 (Peprotech) or with 100 ng/mL of DNP-BSA (thermo-fischer scientific) ...
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bioRxiv - Immunology 2020Quote: ... in the presence of 50 ng/ml granulocyte-macrophage colony-stimulating factor (GM-CSF) and of 20 ng/ml interleukin-4 (IL-4) (PeproTech). DCs stimulation was performed 6 days post-isolation of monocytes ...
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bioRxiv - Immunology 2021Quote: ... Activated C7 cells were concentrated and 4 ml of culture was resuspended in 1 ml RPMI+ containing 10 μg/mL ESAT61-20 peptide + 20 ng/mL IL-12 (Peprotech) + 20 ng/mL IL-2 (Peprotech) ...
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bioRxiv - Microbiology 2021Quote: ... cells were treated with recombinant mouse IFNβ (1000 U/ml, Stratech) or IL-10 (250 ng/ml, catalogue 210-10 Peprotech) 3 h prior to collection for immunoblotting.
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Neutralizing activity of broadly neutralizing anti-HIV-1 antibodies against primary African isolatesbioRxiv - Immunology 2020Quote: ... A total of 2×106 CD4+ T lymphocytes were activated using anti CD3/CD2/CD28 beads (Miltenyi) and cultured in the presence of 100 U/mL IL-2 (Peprotech) at 37 °C and 5% CO2 ...
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bioRxiv - Cell Biology 2021Quote: ... The fused cells were dispersed in 80 ml of GIT medium (Wako, Japan) supplemented with 1 ng/ml IL-6 (PeproTech) and 1×HAT (Kohjin-Bio) ...
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bioRxiv - Immunology 2022Quote: Primary neuronal cells (DIV14) or oligodendrocytes were stimulated with 100 ng/μl of recombinant murine IL-12p70 (#210-12, Peprotech) for 5 min ...
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bioRxiv - Immunology 2022Quote: ... primary neuronal cells were stimulated with 30 ng/μl or 100 ng/μl of Recombinant Murine IL-12p70 (#210-12, Peprotech) for 18 hours ...
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bioRxiv - Immunology 2022Quote: ... or into bone marrow-derived dendritic cells (BMDC) in cRPMI supplemented with recombinant mouse IL-4 and GM-CSF (Peprotech). Influenza virus strains A/California/04/2009 (H1N1) ...
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bioRxiv - Bioengineering 2022Quote: ... and then for three to five days (plasmablast/plasma cell differentiation) in medium containing 50 ng/mL IL-6 (Peprotech),10 ng/mL IL-15 and 15 ng/mL interferon-α2B (Sigma-Aldrich).
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bioRxiv - Immunology 2022Quote: ... The culture conditions used in this study were as follows: HSP condition – cell cultured with IL-15 and -7 (10 μg/ml, PeproTech); HSP+TCR condition – cells cultured with IL-15 and -7 and activated by anti-CD3 (5 μg/ml ...
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bioRxiv - Immunology 2022Quote: ... TCR condition – cells activated with anti-CD3/CD28 antibodies on day 0 and cultured with IL-2 (2 ng/ml, PeproTech); TCR+TCR condition – cells activated with anti-CD3/CD28 on day 0 ...
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bioRxiv - Immunology 2022Quote: ... Th2 cells were generated by activating naïve CD4+ T cells with 1 μg/mL anti-CD3ε/3 μg/mL anti-CD28 for 3 days in complete T cell medium supplemented with 250U recombinant IL-4 (PeproTech), 6 μg/mL anti-IL-12 (BioXcell) ...
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bioRxiv - Physiology 2024Quote: ... the digested cells were first incubated in RPMI 1640 with 10% FBS and 10 ng/ml recombinant murine IL-7 (PeproTech), and stimulated with 50 ng/ml phorbol 12-myristate 13-acetate ...
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bioRxiv - Immunology 2024Quote: ... Cells were expanded for at least 1 week in the presence of IL-3 and 30 ng/mL recombinant SCF (Peprotech) to obtain peritoneal cavity-derived mast cells (PCMCs) ...
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bioRxiv - Immunology 2024Quote: ... Cells were resuspended in complete RMPI (RPMI supplemented with 10% v/v FBS, 100 penicillin, 100 streptomycin) with 50U of IL-2 (PeproTech) and CD3/CD28 Human T-activator Dynabeads (Thermo Fisher ...
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bioRxiv - Immunology 2024Quote: ... The medium was also supplemented with 10 U/ml of DNaseI and 2 ng/mL of recombinant IL-15 (Peprotech). The cell suspension was transferred to a T25 flask (Corning ...
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bioRxiv - Immunology 2024Quote: ... Approximately 5 × 106 NK cells were co-cultured with 1 × 107 of the modified and irradiated K562 feeder cells in 40 mL of Complete NK MACS medium supplemented with 100 U/mL of recombinant IL-2 (Peprotech) in a T75 flask (Corning) ...
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bioRxiv - Microbiology 2024Quote: ... and 1 µg/mL soluble anti-CD28 (clone CD28.2, eBioscience, USA) in the presence of 50 IU/mL IL-2 (PeproTech, USA) for three days ...
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bioRxiv - Cell Biology 2023Quote: ... glutamine and human early-acting cytokines (SCF 300 ng/ml, Flt3-L 300 ng/ml, TPO 100 ng/ml, and IL-3 60 ng/ml; all purchased from Peprotech). All cultures were kept at 37°C in a 5% CO2 water jacket incubator (Thermo Scientific ...
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bioRxiv - Immunology 2024Quote: ... washed one time with PBS and re-plated in a newly irradiated 40LB feeder layer in the presence of 10 ng/ml IL-21 (PeproTech) for the next 4 days ...
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bioRxiv - Immunology 2023Quote: ... Isolated T cells were otherwise activated using plate bound anti-CD3 (5 μg/ml, inVIVOMAb Cat# BE0002) in complete medium added with 100 U/ml IL-2 (Peprotech) and soluble αCD28 (0.5 μg/ml ...
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bioRxiv - Immunology 2023Quote: Isolated CD8+ T cells from OT-I mice were maintained in the naive status after CRISPR-Cas9 gene targeting by resting them overnight in complete medium added with IL-7 (Peprotech) at 10 ng/ml ...
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bioRxiv - Immunology 2022Quote: ... splenocytes from control and Ncr1cre Mycfl/fl mice were left unstimulated or stimulated with 50 ng/mL recombinant mouse IL-15 (PeproTech) for 40 min at 37 °C and analyzed for intracellular protein phosphorylation by flow cytometry ...
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bioRxiv - Bioengineering 2023Quote: ... One half was plated in SCGM medium supplemented with cytokines and 10ng/ml IL-3 (PeproTech, Rocky Hill, NJ, USA) as +IL-3 treatment ...
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bioRxiv - Immunology 2023Quote: ... CD39+ γδ IELs were treated with 100 μM POM-1 (Biotechne) for 2 h prior to the addition of 10 μM ATP (ThermoScientific) and IL-2 (6 ng/ml, Peprotech). Supernatants were collected and stored at -20°C for future use ...
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bioRxiv - Immunology 2023Quote: ... 4-6 x 106 P14 CD8 T cells were plated in 6 well plates and pre-cultured in IL-7 (10ng/ml, PeproTech) at 37°C ...
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bioRxiv - Immunology 2023Quote: ... Cells were resuspended in complete RMPI (RPMI supplemented with 10% v/v FBS, 100 penicillin, 100 streptomycin) with 50U of IL-2 (PeproTech) and CD3/CD28 Human T-activator Dynabeads (Thermo Fisher ...
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bioRxiv - Immunology 2023Quote: ... and light zone cells (CD19+GL7+FAS+CXCR4-CD86+) and cultured in the presence of 200 ng/ml Il-9 (PeproTech) in RPMI media for 3 h at 37°C ...
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bioRxiv - Cancer Biology 2023Quote: ... anti-IL-18 (1 µg/mL, MBL), mIgG1 (1 or 10 µg/mL, R&D) antibodies or IL-1RA (100 ng/mL, Peprotech).
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bioRxiv - Immunology 2023Quote: ... The cells were washed and replated in complete RPMI 1640 medium with 100 U/ml IL-2 (PeproTech, 200-02) 48 hours later ...
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bioRxiv - Biochemistry 2023Quote: ... cells were plated (60,000 cells/well in 24-well plates) two days before treatment in complete media supplemented with 25 ng/mL murine IL-4 (Peprotech) for M2-polarization or without any cytokines (M0) ...
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bioRxiv - Genetics 2023Quote: ... An aliquot of 500,000 cells were plated in 200 µL of media in a 96-well plate and stimulated with 20 ng/mL of IL-6 (Peprotech) for 15 minutes ...
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bioRxiv - Cancer Biology 2023Quote: ... CLL PBMCs were cocultured on irradiated NIH3T3 fibroblasts stably transfected with CD40 ligand or parental cells with or without interleukin-4 (IL-4, 10ng/ml, Peprotech) and IL-21 (25ng/ml ...
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bioRxiv - Bioengineering 2024Quote: ... CD8 T cells were transferred to a new 96-well round bottom plate to remove activation and continued to be cultured in complete RPMI supplemented with a higher concentration of IL-2 (10ng/ml; Peprotech).
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bioRxiv - Immunology 2023Quote: ... beads taken off each well and replated for resting in T cell media containing 10 U/mL of Interleukin-2 (IL-2; Peprotech). To restart each stimulation cycle ...
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bioRxiv - Immunology 2023Quote: ... Cultures were changed on day 4 to new complete RPMI 1640 medium containing recombinant murine IL-5 (10 ng/ml, Peprotech) and further fed with IL-5 every other day between days 10 to 14 ...
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bioRxiv - Immunology 2024Quote: ... Cultures were tested with the indicated concentrations of anti-CD154 (MR-1) or TGF-β+IL-2 (both from Peprotech). In all cases ...
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bioRxiv - Immunology 2024Quote: Naïve and activated CD4+ T cells (via 72h incubation with αCD3/28 beads) were either left untreated or stimulated with IL-10 (40 μg/ml, PeproTech) for 20 min and then lysed with RIPA buffer containing proteases and phosphatases inhibitors (MilliporeSigma) ...
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bioRxiv - Developmental Biology 2024Quote: ... All in vitro T cell generation cultures were supplemented with 5ng/mL Flt3-L and 5 ng/mL IL-7 (Peprotech). Experiments involving in vitro generation of NK and ILC populations (Figures 3 ...
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bioRxiv - Immunology 2024Quote: ... The isolated pDCs (85% purity) were maintained overnight in the presence of IL-3 (1 ng/ml) (PeproTech, London, UK) before stimulation.
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bioRxiv - Immunology 2024Quote: ... For Th1 polarization T lymphocytes were cultured with 5 ng/ml of recombinant murine IL-12 (Peprotech, catalog 210-12). For stimulation of preactivated T lymphocytes with type I IFN ...
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bioRxiv - Cell Biology 2024Quote: ... The medium was collected from wells pre-stimulated with 20 ng/ml TNFα and 20 ng/ml IL-1β (both from PeproTech) for 24 hours ...
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bioRxiv - Bioengineering 2024Quote: IL-12 accessibility to monoclonal antibody binding was determined by using the antibodies in an IL-12 sandwich ELISA kit (Peprotech). After coating a 96 well plate with anti-IL-12 antibodies and blocking the plate with bovine serum albumin according to the manufacturer protocol ...
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bioRxiv - Immunology 2024Quote: ... PBMC were thawed and recovered in complete RPMI overnight without exogenous cytokines before being stimulated with either 1 ng/mL of IL-15 (Peprotech) or 1 ng/mL of IL-15+ 5 ng/mL TGF-β (Peprotech ...
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bioRxiv - Immunology 2024Quote: ... 100 μg/mL streptomycin) containing 30 ng/mL of mouse granulocyte-macrophage colony-stimulating factor (GM-CSF) and Interleukin-6 (IL-6) (PeproTech). After 5 days ...
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bioRxiv - Immunology 2024Quote: ... 100 μg/mL streptomycin) containing 30 ng/mL of mouse granulocyte-macrophage colony-stimulating factor (GM-CSF) and Interleukin-6 (IL-6) (PeproTech) and 4% CHO-SCF conditioned media ...
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bioRxiv - Immunology 2024Quote: ... PBMCs were thawed at 37°C and incubated in complete RPMI media with 10% heat-inactivated FBS and supplemented with IL-2 (50 U/ml; PeproTech). 1 x 104 PBMCs were plated per well in round bottom clear plate (Greiner ...