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Citations for Peprotech :
151 - 199 of 199 citations for 7 8 dimethylbenzo c acridine since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: ... The culture conditions used in this study were as follows: HSP condition – cell cultured with IL-15 and -7 (10 μg/ml, PeproTech); HSP+TCR condition – cells cultured with IL-15 and -7 and activated by anti-CD3 (5 μg/ml ...
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bioRxiv - Immunology 2023Quote: Isolated CD8+ T cells from OT-I mice were maintained in the naive status after CRISPR-Cas9 gene targeting by resting them overnight in complete medium added with IL-7 (Peprotech) at 10 ng/ml ...
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bioRxiv - Immunology 2023Quote: ... 4-6 x 106 P14 CD8 T cells were plated in 6 well plates and pre-cultured in IL-7 (10ng/ml, PeproTech) at 37°C ...
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bioRxiv - Immunology 2023Quote: ... T cells were re-plated with fresh medium containing 10 ng/ml rhIL-2 or a combination of 10 ng/ml IL-7 and IL-15 (all Peprotech) and differentiated at a density of 5×105 cells/ml for additional 4 days ...
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bioRxiv - Cancer Biology 2024Quote: ... according to manufacturer’s specifications in the presence of 100 IU/mL recombinant human (rh) IL-2 and 10 ng/mL rhIL-7 (Peprotech). Spinoculation with neat 293 Galv9 retroviral supernatant was performed at 48 ...
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bioRxiv - Cancer Biology 2024Quote: ... at 3:1 bead: T-cell ratio together with human recombinant IL-15 and IL-7 (PeproTech; 5 ng/mL) in AIM-V medium supplemented with 5% FBS ...
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bioRxiv - Physiology 2024Quote: ... the digested cells were first incubated in RPMI 1640 with 10% FBS and 10 ng/ml recombinant murine IL-7 (PeproTech), and stimulated with 50 ng/ml phorbol 12-myristate 13-acetate ...
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bioRxiv - Cancer Biology 2021Quote: ... or 2) subsequently stimulated with 5 μg/kg T cell chemokine – C-X-C motif chemokine ligand 10 (CXCL10, PeproTech, Rocky Hill, USA) (23) ...
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bioRxiv - Immunology 2021Quote: Both Lin-CD25+CD44- DN3 cells and Lin-CD25-CD44- DN4 cells were sorted from the thymi of LckCre+Zfp335+/+ and LckCre+Zfp335fl/fl mice and co-cultured with OP9-DL1 feeder cells in α-MEM medium in the presence of IL-7 (1 ng/ml, PeproTech) and Flt3-L (5 ng/ml ...
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bioRxiv - Immunology 2022Quote: ... and differentiated for 7 days in Iscove’s Modified Dulbecco’s Media (IMDM) supplemented with 20 ng/ml of M-CSF (Peprotech, Rocky Hill, NJ), 10% heat-inactivated fetal bovine serum (FBS ...
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bioRxiv - Immunology 2024Quote: ... A single round of co-sedimentation with retroviral stock was performed in medium containing 5% WEHI-3B-conditioned medium and 10 ng/mL IL-7 (Peprotech, USA). After transduction ...
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bioRxiv - Immunology 2021Quote: ... Inducers of neutrophil chemotaxis were added to the bottom well in 600 µL of RPMI-1640 media containing 1,000 or 50,000 pg of recombinant human (rh-) IL-8 (PeproTech) or A549 cells culture previously infected for 48 hours with mock or ZIKV PE243 (1 MOI) ...
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bioRxiv - Cell Biology 2023Quote: ... splenocytes of 6-8-week-old mice were stimulated with 20 ng/mL recombinant mouse IL-12 (Peprotech) in combination with 200 ng/mL recombinant mouse IL-18 (Peprotech ...
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bioRxiv - Immunology 2021Quote: ... cells were incubated for the indicated time in the absence or presence of IL-7 (10 ng/ml, Peprotech, Cat#: 200-07) or stem cell factor (20 ng/ml ...
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bioRxiv - Immunology 2022Quote: ... Cells were transdifferentiation for 7 days at 60,000 cells/cm2 in medium containing 50 ng/ml IL-3 (200-03 B, Peprotech, New Jersey, USA), 50 ng/ml M-CSF (300-25 B ...
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bioRxiv - Immunology 2022Quote: ... primary BMDMs were grown for 7 days in complete RPMI media containing 20 ng/mL of recombinant MCSF (Peprotech, Rocky Hill, NJ) prior to use in assays ...
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bioRxiv - Cancer Biology 2019Quote: ... from day 10 onward cultures were maintained at 50 U/ml IL-7 and IL-15 (PeproTech, Rocky Hill, NJ #200-07). T cells that had been activated but not transduced were used as mock T control ...
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bioRxiv - Bioengineering 2022Quote: ... Hydrogels were cultured in 48 well plates for 7 days and maintained in ECM with or without additional growth factors (± 100 ng/mL recombinant human VEGF165; PeproTech 100-20) and hormones ...
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bioRxiv - Cancer Biology 2024Quote: ... according to the manufacturer’s instructions (20 μL of beads per 1 million T-cells) for 5 days and grown in the presence of recombinant interleukin-7 (PeproTech, 200-07) and interleukin-15 (PeproTech ...
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bioRxiv - Cancer Biology 2024Quote: ... according to the manufacturer’s instructions (20 μL of beads per 1 million T-cells) for 5 days and grown in the presence of recombinant interleukin-7 (PeproTech, 200-07) and interleukin-15 (PeproTech ...
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bioRxiv - Cancer Biology 2024Quote: ... One was supplemented with IL-2 at 300 IU/ml (BioTechne, BT-002-AFL) and the other was supplemented with IL-7 at 20ng/ml (PeproTech, 200-07). The cells were activated with 100µl a-CD3/a-CD8 nanoparticles (TransAct ...
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bioRxiv - Cell Biology 2023Quote: ... The Rudibaugh forebrain organoid media was supplemented with 100ng/mL of DKK1 and the Rudibaugh midbrain organoid media was supplemented with 3µM CHIR99021 and 100ng/mL FGF-8(Peprotech). On D20 ...
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bioRxiv - Cancer Biology 2022Quote: ... Cells were then cultured on untreated plates for 7 days adding 10 ng/ml recombinant murine M-CSF (Peprotech, AF-315-02-100) at day of isolation ...
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bioRxiv - Biochemistry 2020Quote: ... At day 8 cells were detached and aggregated in the U bottom microwells in the presence of 20 ng/mL HGF (PeproTech), 10 ng/mL oncostatin M (OSM ...
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bioRxiv - Genomics 2020Quote: ... 1% Glutamax, and 1% FBS, 8 ng/ml FGF2, 5 ng/ml CNTF, and 10 ng/ml BMP4, from Peprotech). On day 7 ...
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bioRxiv - Cell Biology 2021Quote: Primary mouse AT2 cells were isolated from homozygous H991A adult (mice 6-8 weeks) and cultured in BEGM plus 10% charcoal-stripped FBS and recombinant human KGF (10 ng/ml, Peprotech) on top of a thin substrate of 70% Cultrex (Trevigen)/30% rat tail collagen (Advanced Biomatrix ...
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bioRxiv - Cancer Biology 2019Quote: ... BMDM were incubated overnight at 37°C with TGFβ (Peprotech; 5ng/ml), the ROS inhibitor NacetylCysteine (ThermoFicsher ...
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bioRxiv - Cell Biology 2022Quote: ... were cultured at a ratio of red:green (1:1) in 8-well chamber slides (ThermoFischer Scientific, Cat # 154534) and stimulated with M-CSF (20 ng/ml, PeproTech, UK) for 3 days ...
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bioRxiv - Neuroscience 2023Quote: ... and plated on poly-L-ornithine/laminin (PLO)-coated dishes at a density of 10,000–15,000 cells/cm2 in N2B27 medium supplemented with 100 ng/ml FGF-8 (PeproTech, Cat.100-25), 200 ng/ml sonic hedgehog (PeproTech ...
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bioRxiv - Immunology 2021Quote: ... Sorted cells were seeded in 10 cm tissue-culture dishes of 80 – 90% confluent OP9 cells containing 5 ng/ml Flt-3L (R&D, Catalogue no.-427-FL) and 1 ng/ml IL-7 (PeproTech, Catalogue no.- 217-17-10). For T-cell development assay ...
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bioRxiv - Immunology 2022Quote: ... OT-I T cells were activated with 10-8 SIINFEKL or SIITFEKL (Cambridge Peptides) in the presence or absence of recombinant human TGFβ (Peprotech, 5ng/ml). Where indicated ...
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bioRxiv - Cancer Biology 2023Quote: ... Activated T-cells were kept in medium supplemented with IL-7 and IL-15 (#200-07 and #200-15, PeproTech, 10 and 5 ng/mL, respectively). Gamma-retroviral transduction was facilitated by RetroNectin (#T100B ...
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bioRxiv - Cell Biology 2022Quote: ... Following incubation for 24 h at 37°C with or without VEGFA (50 ng/ml; Peprotech) the gels were fixed with 10% formaldehyde (Roth ...
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bioRxiv - Cancer Biology 2023Quote: ... or c) supplemented with recombinant human IGFBP5 protein (500 ng/ml; Peprotech, Rocky Hill, NJ, USA). Cell lysates were obtained using RIPA buffer with the Halt Protease and Phosphatase Inhibitor Cocktail (Thermo Fisher ...
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bioRxiv - Immunology 2022Quote: ... the cells were resuspended in pre-warmed (37°C) recombinant IL-12-containing (20 ng/ml; PeproTech) medium for 15 min to induce phosphorylation of STAT4 ...
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bioRxiv - Developmental Biology 2022Quote: ... for two hours at 37°C with StemFlex medium and 10 μM Y-27632 (ROCK inhibitor, Peprotech). The plate was put in an incubator at 37°C and 5% CO2 for 30 minutes to ensure cell attachment ...
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bioRxiv - Developmental Biology 2022Quote: ... for two hours at 37°C with StemFlex medium and 10 μM Y-27632 (ROCK inhibitor, Peprotech). The plate was put in an incubator at 37°C and 5% CO2 for 30 minutes to ensure cell attachment ...
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bioRxiv - Microbiology 2020Quote: ... Bone marrow cells were cultivated for 10 days at 37 °C in the presence of GM-CSF (Peprotech) 43 ...
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bioRxiv - Immunology 2022Quote: ... and cultured at 37 °C in 5% CO2 with the indicated amounts of recombinant mouse IL-15 (PeproTech).
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bioRxiv - Immunology 2021Quote: ... for 4 days at 37°C in complete RPMI medium supplemented with 10 ng/mL recombinant mouse IL-5 (PeproTech) and 20 ng/mL IL-2 (402-ML R&D) ...
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bioRxiv - Immunology 2021Quote: ... for 4 days at 37°C in complete RPMI medium supplemented with 10 ng/mL recombinant mouse IL-5 (PeproTech) and 20 ng/mL IL-2 (402-ML R&D) ...
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bioRxiv - Immunology 2022Quote: ... we thawed cryopreserved TCRα/β-depleted BM fractions at 37°C and cultured them overnight in SFEM containing 100 ng/mL human stem cell factor (SCF, Peprotech), 100 ng/mL human Fms-related tyrosine kinase 3 (FLT3 ...
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bioRxiv - Immunology 2023Quote: ... splenic NK cells were MACs enriched and cultured at 37°C and 5% CO2 in RPMI containing 300U rIL-2 (Peprotech), 50ng/ml rIL-15 (Peprotech ...
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bioRxiv - Immunology 2024Quote: ... cells were thawed and rested in an 37°C / 5% CO2 incubator in the T cell medium with 50 U/mL rhIL-2 (Peprotech, Thermo Fisher Scientific ...
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bioRxiv - Immunology 2023Quote: ... single-cell suspensions of spleens from 10-week-old Foxp3Thy1.1 mice were stimulated for 15 minutes at 37°C with the indicated concentration of recombinant mouse IL2 (PeproTech, 212-12) in serum-free RPMI 1640 ...
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bioRxiv - Immunology 2022Quote: ... Cells were incubated for 3 hours at 37°C in RPMI media supplemented with or without recombinant mouse IL-21 (20 ng/mL, Peprotech cat. 210-21-100). Cells were stimulated with either anti-Igκ and anti-Igλ or anti-CD40 ...
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bioRxiv - Immunology 2022Quote: ... hCD34+ cells were either kept in culture at 37°C in RPMI medium with human recombinant Stem Cell Factor (SCF) at 50 ng/ml (PeproTech, Rocky Hill, NJ, USA), human recombinant thrombopoietin (TPO ...
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bioRxiv - Cell Biology 2020Quote: ... They were grown under standard conditions at 37°C and 5% CO2 with NeuroCult basal media (STEMCELL, Vancouver, BC, CA) with EGF (50 ng/ml, PeproTech, Inc., Rocky Hill, NJ, USA), FGF (100 ng/ml ...
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Toxoplasma GRA15 limits parasite growth in IFNγ-activated fibroblasts through TRAF ubiquitin ligasesbioRxiv - Immunology 2020Quote: ... MEFs were also stimulated for 18-24 h in complete medium with HEPES at 37 °C with 100 U/mL murine IFNγ (Peprotech, stock concentration is 100,000 U/mL).