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Citations for Peprotech :
151 - 196 of 196 citations for 27 Carboxy 7 keto Cholesterol d4 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Synthetic Biology 2020Quote: ... cells were spun down and supernatants were collected and analyzed by ELISA for IL-7 following the manufacturer’s recommended protocol (Peprotech).
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bioRxiv - Immunology 2022Quote: ... followed by addition of 100,000 sorted CD5- or CD71- DN3 thymocytes per well together with 10 ng/ml recombinant mouse IL-7 (PeproTech).
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bioRxiv - Immunology 2024Quote: ... T cells were removed from stimulation and cultured in complete medium supplemented with10ng/ml recombinant mouse IL-7 (Peprotech) for 3-5 days as described (69).
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bioRxiv - Immunology 2023Quote: ... at a 1:1 bead:cell ratio in complete RPMI supplemented with 5 ng/mL IL-7 and IL-15 (both PeproTech) and after 48-72 h ...
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bioRxiv - Cancer Biology 2022Quote: ... 1 µM of each synthetic peptide (>90% purity, DGPeptidesCo Ltd.) was added to the culture along with 0.5 ng/ml Interleukin (IL)-7 (Peprotech), 50 ng/ml Tumor necrosis factor (TNF)-α (Peprotech ...
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bioRxiv - Immunology 2024Quote: ... cells from C57BL/6 and CD11cdnR spleens were cultured for 7 days with 10 ng/ml IL-15 (PeproTech) supplemented with or without 10 ng/ml TGFβ1 (R&D) ...
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bioRxiv - Immunology 2021Quote: ... with 2% FBS (HyClone Thermo Fischer Scientific) for 15 minutes with or without murine IL-7 (50 ng/mL, PeproTech). Following stimuli ...
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bioRxiv - Immunology 2022Quote: Cells were cultured in complete T cell media supplemented with 5 ng/mL each of recombinant human IL-7 and IL-15 (PeproTech) and 10 U/mL recombinant human IL-2 ...
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bioRxiv - Immunology 2022Quote: Cells were cultured in complete T cell media supplemented with 5 ng/mL each of recombinant murine IL-7 and IL-15 (PeproTech). One day prior to activation of OT-I cells ...
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bioRxiv - Cancer Biology 2020Quote: ... Ba/F3 cells and Ba/F7 cells were maintained in medium supplemented with 10 ng/ml recombinant mouse interleukin 3 (IL3) and interleukin 7 (IL7) (PeproTech), respectively ...
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bioRxiv - Immunology 2022Quote: ... OT-I T cells were maintained inactivated in vitro (‘No TCR’ condition) with recombinant mouse IL-7 (Peprotech, 10ng/ml). For IL-2 replenishment experiments ...
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bioRxiv - Cancer Biology 2022Quote: ... mAb in 200ul of T cell media supplemented with 5 ng/ml of IL-7 and 30 U/ml of IL-2 (PeproTech) for 4 and 7 days ...
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bioRxiv - Immunology 2020Quote: ... 5 ng/ml of IL-2 was added to the culture and memory T cells were supplemented with 5 ng/ml IL-7 and IL-15 (all Peprotech). Cells were activated in a reverse time-course so that their total time in culture was identical and so that all cells would finish their indicated activation period at the same day to enable extracellular flux analysis ...
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bioRxiv - Cancer Biology 2021Quote: ... bone marrow cells were obtained from 8-10 weeks old BALB/c mice and cultured for 7 days in DMEM supplemented with 10% FBS and 20 μg/ml macrophage-colony stimulating factor (M-CSF, Peprotech). All medium contained 50 U/ml penicillin and 50 μg/ml streptomycin (Sigma-Aldrich).
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bioRxiv - Immunology 2020Quote: ... These cells were cultured for 7 d in DMEM cell culture medium containing 40 ng/ml macrophage colony-stimulating factor (M-CSF) (PeproTech). For the generation of bone marrow-derived dendritic cells ...
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bioRxiv - Immunology 2021Quote: Bone marrow cells were isolated from C57BL/6J mouse tibia and femur and then cultured for 7–9 days in 10% FBS DMEM containing mouse GM-CSF (50 ng/mL, Peprotech).
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bioRxiv - Immunology 2021Quote: ... peptide-reactive T-cell lines from day 925 and 945 were expanded in culture for two weeks with IL-7 and IL-15 (5ng/mL; Peprotech) and cultured for 24h with irradiated (100Gy ...
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bioRxiv - Immunology 2023Quote: Isolated CD8+ T cells from OT-I mice were maintained in the naive status after CRISPR-Cas9 gene targeting by resting them overnight in complete medium added with IL-7 (Peprotech) at 10 ng/ml ...
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bioRxiv - Immunology 2023Quote: ... 4-6 x 106 P14 CD8 T cells were plated in 6 well plates and pre-cultured in IL-7 (10ng/ml, PeproTech) at 37°C ...
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bioRxiv - Immunology 2023Quote: ... T cells were re-plated with fresh medium containing 10 ng/ml rhIL-2 or a combination of 10 ng/ml IL-7 and IL-15 (all Peprotech) and differentiated at a density of 5×105 cells/ml for additional 4 days ...
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bioRxiv - Physiology 2024Quote: ... the digested cells were first incubated in RPMI 1640 with 10% FBS and 10 ng/ml recombinant murine IL-7 (PeproTech), and stimulated with 50 ng/ml phorbol 12-myristate 13-acetate ...
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bioRxiv - Cancer Biology 2024Quote: ... at 3:1 bead: T-cell ratio together with human recombinant IL-15 and IL-7 (PeproTech; 5 ng/mL) in AIM-V medium supplemented with 5% FBS ...
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bioRxiv - Cancer Biology 2024Quote: ... according to manufacturer’s specifications in the presence of 100 IU/mL recombinant human (rh) IL-2 and 10 ng/mL rhIL-7 (Peprotech). Spinoculation with neat 293 Galv9 retroviral supernatant was performed at 48 ...
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bioRxiv - Cancer Biology 2022Quote: ... Enriched cells were taken into culture in T cell medium (TCM, see Methods – primary human material and cell lines) supplemented with 5 ng/mL IL-7 (Peprotech), 5 ng/mL IL-15 (Peprotech) ...
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bioRxiv - Immunology 2022Quote: ... The culture conditions used in this study were as follows: HSP condition – cell cultured with IL-15 and -7 (10 μg/ml, PeproTech); HSP+TCR condition – cells cultured with IL-15 and -7 and activated by anti-CD3 (5 μg/ml ...
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bioRxiv - Developmental Biology 2024Quote: ... All in vitro T cell generation cultures were supplemented with 5ng/mL Flt3-L and 5 ng/mL IL-7 (Peprotech). Experiments involving in vitro generation of NK and ILC populations (Figures 3 ...
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bioRxiv - Immunology 2024Quote: ... BMDC and neutrophils were generated in 7-day cultures with complete RPMI-10 supplemented with 15ng/mL recombinant GM-CSF (PeproTech). Cells from the floating fraction were harvested and BMDC were sorted using anti-CD11c MicroBeads (Miltenyi Biotech ...
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bioRxiv - Immunology 2024Quote: ... splenocytes were adjusted to a concentration of 3 x 106 cells/mL in T cell medium containing 10 ng/mL rhIL-7 (Peprotech) and 2 µg/mL anti-mouse CD28 (clone 37.51 ...
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bioRxiv - Immunology 2024Quote: ... T cells were removed from stimulation and cultured in complete medium supplemented with 10 ng/ml recombinant mouse IL-7 (Peprotech) for 3-5 days as previously described (39) ...
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bioRxiv - Physiology 2024Quote: ... day 7 cells were dissociated with Accutase and plated at a density of ∼5 × 104 cells/cm2 in laminin-511 (PeproTech)-coated plates with podocyte induction media ...
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bioRxiv - Immunology 2021Quote: Both Lin-CD25+CD44- DN3 cells and Lin-CD25-CD44- DN4 cells were sorted from the thymi of LckCre+Zfp335+/+ and LckCre+Zfp335fl/fl mice and co-cultured with OP9-DL1 feeder cells in α-MEM medium in the presence of IL-7 (1 ng/ml, PeproTech) and Flt3-L (5 ng/ml ...
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bioRxiv - Immunology 2022Quote: ... and differentiated for 7 days in Iscove’s Modified Dulbecco’s Media (IMDM) supplemented with 20 ng/ml of M-CSF (Peprotech, Rocky Hill, NJ), 10% heat-inactivated fetal bovine serum (FBS ...
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bioRxiv - Immunology 2024Quote: ... A single round of co-sedimentation with retroviral stock was performed in medium containing 5% WEHI-3B-conditioned medium and 10 ng/mL IL-7 (Peprotech, USA). After transduction ...
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bioRxiv - Immunology 2021Quote: ... cells were incubated for the indicated time in the absence or presence of IL-7 (10 ng/ml, Peprotech, Cat#: 200-07) or stem cell factor (20 ng/ml ...
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bioRxiv - Immunology 2022Quote: ... Cells were transdifferentiation for 7 days at 60,000 cells/cm2 in medium containing 50 ng/ml IL-3 (200-03 B, Peprotech, New Jersey, USA), 50 ng/ml M-CSF (300-25 B ...
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bioRxiv - Immunology 2022Quote: ... primary BMDMs were grown for 7 days in complete RPMI media containing 20 ng/mL of recombinant MCSF (Peprotech, Rocky Hill, NJ) prior to use in assays ...
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bioRxiv - Cancer Biology 2024Quote: ... One was supplemented with IL-2 at 300 IU/ml (BioTechne, BT-002-AFL) and the other was supplemented with IL-7 at 20ng/ml (PeproTech, 200-07). The cells were activated with 100µl a-CD3/a-CD8 nanoparticles (TransAct ...
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bioRxiv - Cancer Biology 2024Quote: ... according to the manufacturer’s instructions (20 μL of beads per 1 million T-cells) for 5 days and grown in the presence of recombinant interleukin-7 (PeproTech, 200-07) and interleukin-15 (PeproTech ...
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bioRxiv - Cancer Biology 2024Quote: ... according to the manufacturer’s instructions (20 μL of beads per 1 million T-cells) for 5 days and grown in the presence of recombinant interleukin-7 (PeproTech, 200-07) and interleukin-15 (PeproTech ...
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bioRxiv - Bioengineering 2022Quote: ... Hydrogels were cultured in 48 well plates for 7 days and maintained in ECM with or without additional growth factors (± 100 ng/mL recombinant human VEGF165; PeproTech 100-20) and hormones ...
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bioRxiv - Bioengineering 2024Quote: ... in T75 flasks at 2 million/mL and differentiated for 7-9 days with 50 ng/mL recombinant human M-CSF (300-25, PeproTech, Cranbury, NJ) and 20 ng/mL recombinant human IL-4 (200-04 ...
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bioRxiv - Cancer Biology 2022Quote: ... Cells were then cultured on untreated plates for 7 days adding 10 ng/ml recombinant murine M-CSF (Peprotech, AF-315-02-100) at day of isolation ...
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bioRxiv - Immunology 2024Quote: ... Cells were co-cultured for 14 days in AimV medium enriched with IL-7 (Miltenyi Cat.130-095-363) and IL-15 (Peprotech Cat.200-1s), changing media periodically and restimulating a second time with peptide-pulsed MoDCs after 7 days ...
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bioRxiv - Immunology 2024Quote: ... cells were cultured in RPMI 1640 supplemented with 10% FBS and 1X Pen/Strep in the presence of either 1 ng/mL Recombinant murine IL-7 (PeproTech, Cat#217-17) or stimulated with anti-CD3/CD28 Dynabeads at a 4:1 bead:cell ratio (Fisher Scientific ...
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bioRxiv - Immunology 2021Quote: ... Sorted cells were seeded in 10 cm tissue-culture dishes of 80 – 90% confluent OP9 cells containing 5 ng/ml Flt-3L (R&D, Catalogue no.-427-FL) and 1 ng/ml IL-7 (PeproTech, Catalogue no.- 217-17-10). For T-cell development assay ...
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bioRxiv - Cancer Biology 2023Quote: ... Activated T-cells were kept in medium supplemented with IL-7 and IL-15 (#200-07 and #200-15, PeproTech, 10 and 5 ng/mL, respectively). Gamma-retroviral transduction was facilitated by RetroNectin (#T100B ...