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Citations for Peprotech :
1901 - 1950 of 1973 citations for CU 115 CAS 2471982 20 2 98% since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2024Quote: ... PBMCs were thawed at 37°C and incubated in complete RPMI media with 10% heat-inactivated FBS and supplemented with IL-2 (50 U/ml; PeproTech). 1 x 104 PBMCs were plated per well in round bottom clear plate (Greiner ...
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bioRxiv - Immunology 2024Quote: ... Samples were stimulated and incubated overnight in complete RPMI media with 10% heat-inactivated FBS and supplemented with IL-2 (50 U/ml; PeproTech).
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bioRxiv - Immunology 2024Quote: ... or the MoFlo XDP cell sorter and rested for 24 hrs in medium containing rIL-2 (10U/ml, cat. 212-12 Peprotech) before activation or adoptive transfer into the animals.
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bioRxiv - Immunology 2024Quote: Single cell suspensions from tumor tissue were isolated as described above and plated in cRPMI containing either 50U/mL IL-2 or 1ng/mL IL-15 (Peprotech) in 12-well culture plates ...
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bioRxiv - Immunology 2024Quote: ... Cultures were tested with the indicated concentrations of anti-CD154 (MR-1) or TGF-β+IL-2 (both from Peprotech). In all cases ...
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bioRxiv - Immunology 2024Quote: ... 1×106 differentiated iRBCs or undifferentiated HUDEP-2 cells were stimulated with 50 ng/mL recombinant human interferon-gamma (PeproTech) for 6 hours ...
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bioRxiv - Immunology 2024Quote: ... KHYG1 cells were then single-cell sorted into 96-well plates and grown in RPMI-1640 media supplemented with recombinant human IL-2 1000 U/mL (Peprotech) for 5-6 weeks ...
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bioRxiv - Immunology 2024Quote: ... 100 μg/ml streptomycin and 100 units/ml penicillin) and stimulated with plate-bound anti-CD3/CD28 in the presence of 10 ng/ml IL-2 (Peprotech) as indicated ...
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bioRxiv - Immunology 2024Quote: ... Siglec-Fhi and Siglec-Flow neutrophils were prepared by culturing bone marrow neutrophils for 2 days in the presence or absence of 10 ng/ml GM-CSF (Peprotech) and 10 ng/ml IL-4 (R&D Systems) ...
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bioRxiv - Immunology 2024Quote: ... Every 48 hours the cells were centrifuged and the pellet was resuspended between 1 and 2 × 106 per mL in fresh complete media containing the OVA peptide 3 μg/mL and IL-2 (100 U/mL) after the second stimulation IL-15 (10 ng/mL, Peprotech) was added The number of stimulations is indicated in the figure/text ...
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bioRxiv - Immunology 2024Quote: ... Human peripheral blood mononuclear cells (PBMCs) were preactivated with 30 ng/mL anti-CD3 (OKT3, Miltenyi) and 50 IU/mL IL-2 (PeproTech) and subsequently transduced two times with viral supernatant in the presence of 6 μg/mL polybrene (Sigma ...
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bioRxiv - Immunology 2024Quote: ... NK cells were cultured in U-bottom 96-well plates in cRPMI containing 100 U/ml of IL-2 (Peprotech) for 2 days ...
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bioRxiv - Immunology 2021Quote: ... when the cells were stimulated with 1000 U/ml of rhIL-2 and the combination of 10 ng/ml of recombinant murine (rm) IL-12 (210-12, PeproTech Inc) and 40 ng/ml of rm IL-15 (210-15 ...
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bioRxiv - Developmental Biology 2020Quote: ... DFs cultured in DMEM/F12 with 2% FBS supplemented with TGFβ1 or TGFβ3 (10 ng/ml; PeproTech EC, Ltd., London, UK) or 5 mM citric acid (diluent for lyophilized TGFNβ ...
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bioRxiv - Immunology 2021Quote: ... 1x GlutaMAX™, 50µM 2-mercaptoethanol, 100 units/ml penicillin, and 100µg/ml streptomycin, 10ng/ml mIL-21 PeproTech 210-21). On day8 B cells were harvested and analysed.
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bioRxiv - Bioengineering 2021Quote: ... and re-plated for 2 additional passages at a density of 5 × 103 cells/cm2 at 5% pO2 in expansion medium supplemented with 5 ng/ml of fibroblast growth factor (FGF)-2 (PeproTech Ltd). Medium change was performed three times per week.
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bioRxiv - Cell Biology 2022Quote: ... Recombinant human fibroblast growth factor (FGF-2, catalog no. 100-18B) and transforming growth factor beta (TGF-β, catalog no. 100-21) were purchased from PeproTech, Inc ...
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bioRxiv - Neuroscience 2022Quote: ... then 4 million cells were seeded per well in 2 ml of EB Medium: complete mTeSR + 0.05 ug/ml BMP-4 (PeproTech, #120-05) + 0.05 ug/ml VEGF (PeproTech ...
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bioRxiv - Cancer Biology 2022Quote: ... PBMCs were harvested from K3EDTA anti-coagulated whole blood by density gradient centrifugation with Lymphoprep and plated in 12 well plates at 2-4 × 106 cells per well in 10% RPMI + 10 ng/mL human CSF (300-25, Peprotech, UK). Medium was replaced every 2-3 days until adherent populations of macrophages had established ...
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bioRxiv - Immunology 2024Quote: ... Transduced cells were incubated for 4 days with plate-bound anti-CD3/anti-CD28 in media supplemented with hIL-2 (0.0344 units/mL, Peprotech #200-02). Transduction efficiency was assessed for each experiment by flow cytometry.
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bioRxiv - Cell Biology 2023Quote: ... at a density of 0.15×106 cell/well and cultured for 2 days in supplemented α-MEM with 30 ng/ml M-CSF (PeproTech, UK). Media were replaced with α-MEM supplemented with 30 ng/ml M-CSF and 30 ng/ml RANKL (PeproTech ...
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bioRxiv - Immunology 2024Quote: ... China) gradient and pulsed with 0.5 µM rUreB protein in the presence of 5 U/ml rmIL-2 (PeproTech, NJ, USA). Cells were cultured in “RF-10” medium consisting of RPMI 1640 (Gibco ...
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bioRxiv - Immunology 2024Quote: ... 50 μM 2-mercaptoethanol) with 100 ng/ml of human FMS-like tyrosine kinase 3 ligand (FLT3L, PeproTech, Cranbury, NJ, USA) for 7 days ...
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bioRxiv - Cell Biology 2020Quote: ... They were grown under standard conditions at 37°C and 5% CO2 with NeuroCult basal media (STEMCELL, Vancouver, BC, CA) with EGF (50 ng/ml, PeproTech, Inc., Rocky Hill, NJ, USA), FGF (100 ng/ml ...
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bioRxiv - Developmental Biology 2020Quote: ... the culture medium was replaced with fresh DMEM/F12 with 2% FBS supplemented with TGFβ1/TGFβ3 (10 ng/ml; PeproTech EC, Ltd., London, UK) or 5mM citric acid (diluent for lyophilized TGFβ ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were deprived of serum for 2h and stimulated with 2 ng/ml of recombinant human EGF (Peprotech; Catalog #AF-100-15) for 2 ...
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bioRxiv - Bioengineering 2020Quote: ... 50 uL HS (250 mM NaCl) and PS (145 mM NaCl) gels (10 mg/mL fibrinogen) were fabricated with 100 ng/mL FGF-2 (Peprotech, 100-18b) and 100 ng/mL VEGF-165 (Shenandoah Biotechnology ...
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bioRxiv - Bioengineering 2020Quote: ... Primary human T cells were cultured in complete RPMI 1640 supplemented with 100 U/mL recombinant human IL-2 (PeproTech, 200-02). Cells were cultured at 37°C in a humidified 5% CO2 incubator.
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bioRxiv - Systems Biology 2020Quote: ... 2 mM glutamine and 10% fetal calf serum in the presence of 50 ng/ml of M-CSF (Peprotech, cat# 300-25). 5 × 105 CD14+CD16− monocytes or × 105 resting macrophages were activated with 10 ng/ml of lipopolysaccharides (LPS ...
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bioRxiv - Immunology 2021Quote: ... cells were starved for 6 hours in fresh media lacking cytokines and subsequently treated with 100 U/mL of either IL-2 or IL-15 (PeproTech 200-15).
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bioRxiv - Microbiology 2020Quote: ... Frozen activated PBMC cells were thawed and resuspended in complete PBMC media containing RPMI supplemented with 21 ng/mL human IL-2 (Peprotech; #200-02), 10% fetal bovine serum and 1% penicillin-streptomycin ...
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bioRxiv - Microbiology 2020Quote: ... Pellets were resuspended in activation medium containing RPMI supplemented with 21 ng/mL human IL-2 (Peprotech, Rocky Hill, NJ; #200-02), 1.5 μg/mL PHA (Remel ...
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bioRxiv - Developmental Biology 2022Quote: ... The cells were cultured on pre-coated plates with 0.5% growth factor-reduced Matrigel at a density of 2×104 cells/cm2 with MEF-conditioned medium supplemented with 10 ng/ml BMP4 (Peprotech, 120-05ET), 1µM A83-01 ...
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bioRxiv - Systems Biology 2020Quote: ... on Day 1 we changed the medium to RPMI + 2% B-27 (minus insulin) + 5ng/ml BMP4 (Peprotech, cat. AF-120-05ET) + 1uM Chiron 99021 and incubated these cells for 48 hours ...
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bioRxiv - Neuroscience 2020Quote: For the ADV transduction experiments human iPSC derived NSCs were plated at 700,000 cells per well of a 6-well plate in 2 ml NPM supplemented with 25 ng/ml βFGF (Peprotech, 100-18B) and 25 ng/ml Activin A (Peprotech ...
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bioRxiv - Pathology 2020Quote: ... HUVEC were plated at a density of 10,000 cells/96-well plate in EGM-2 supplemented with recombinant human TNF-α (300-01A, PeproTech EC Ltd), IL1-β (200-01B ...
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bioRxiv - Immunology 2021Quote: ... Activation was carried out at a cell density of 1M/ml in 100 U/ml Recombinant Murine IL-2 (Peprotech 212-12). Dynabeads™ Mouse T-Activator CD3/CD28 (ThermoFisher 11453D ...
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bioRxiv - Immunology 2022Quote: ... coated with anti-CD3 (4 μg/ml) and anti-CD28 (4 μg/ml) in the presence of IL-2 (Peprotech, 50ng/ml). CD19+ B cells were stimulated for 24 hours using Purified F(ab’)2 Goat anti-mouse IgM Antibody (BioLegend) ...
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bioRxiv - Bioengineering 2024Quote: ... Cells were then moved to a flat-bottom 96 well plate containing 160 uL of medium supplemented with 500 U/mL IL-2 (Peprotech, Cranbury, NJ) or 10 ng/mL of IL-7 (BioTechne ...
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bioRxiv - Immunology 2024Quote: ... cells were isolated from bone marrow of C57Bl/6 mice and cultured with granulocyte-macrophage stimulating factor (100ng or 2×103 units per 3×106 cells, Peprotech, Cranbury, NJ) in RPMI-1640 media containing 10% heat inactivated fetal bovine serum ...
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bioRxiv - Bioengineering 2024Quote: ... Isolated PB-NK cells were expanded with irradiated K562-mb15-41BBL feeder cells (1-3 days before NK cell isolation, 100 Gy) along with 50 IU/mL IL-2 (Peprotech, Cranbury, NJ) and 2 ng/mL IL-15 (Peprotech ...
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bioRxiv - Systems Biology 2023Quote: B2B1 cells were seeded on tissue culture plastic (6-well to 15-cm plates) at 12,500 cells/cm2 in assay medium + 2% matrigel + 5 ng/ml EGF (Peprotech, AF-100-15) and refed every four days ...
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bioRxiv - Immunology 2023Quote: All cell lines were seeded in flat 96-well plates at a density of 0.25 × 105 cells/well and cultured for 24 h in the absence or presence of human recombinant IFNγ and TNFα with activities of 2 × 107 Units/mg (PeproTech, Rehovot, Israel). Cells lines were then co-cultured with 100,000 (at density of 1×106 cell/ml ...
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bioRxiv - Bioengineering 2023Quote: ... Primary human T cells were cultured in complete RPMI 1640 supplemented with 100□U/ml recombinant human IL-2 (PeproTech, 200-02). All mammalian cells were cultured at 37□°C in a humidified 5% CO2 incubator.
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bioRxiv - Developmental Biology 2023Quote: ... cells were washed with PBS and further cultured for two days in S1/2 medium supplemented with FGF7 (50ng/ml) (PeproTech, 100-19) and vitamin C (0.25mM ...
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bioRxiv - Immunology 2023Quote: ... TIL or KSQ-001 cells were added to spheroid plate in 100μL REP media supplemented with 6000IU/mL IL-2 (Peprotech, Cat#200-02). Images were taken via Incucyte S3 (Sartorius ...
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bioRxiv - Developmental Biology 2024Quote: ... DE cells were rinsed with PBS and then exposed to S1/2 medium supplemented with 50 ng/ml KGF (FGF7) (PeproTech, 100-19) and 0.25 mM vitamin C (VitC ...
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bioRxiv - Neuroscience 2024Quote: ... one well of dissRO and iMG-dissRO were treated with recombinant human CCL2 (C-C Motif Chemokine Ligand 2, Peprotech, #300-04) at a final concentration of 10 ng/mL ...
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bioRxiv - Biochemistry 2024Quote: ... Each diluted suspension was carefully mixed and further diluted to 2 ml using OPC differentiation media (antibiotic-free DMEM-Sato supplemented with 10 ng/ml CNTF (Peprotech 450-13), 10 µM forskolin (Sigma-Aldrich F6886 ...
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bioRxiv - Bioengineering 2024Quote: ... in T75 flasks at 2 million/mL and differentiated for 7-9 days with 50 ng/mL recombinant human M-CSF (300-25, PeproTech, Cranbury, NJ) and 20 ng/mL recombinant human IL-4 (200-04 ...