Labshake search
Citations for Peprotech :
1851 - 1900 of 1966 citations for Human TGF beta 3 TGFB3 qPCR Primer Pair since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Microbiology 2020Quote: ... Pellets were resuspended in activation medium containing RPMI supplemented with 21 ng/mL human IL-2 (Peprotech, Rocky Hill, NJ; #200-02), 1.5 μg/mL PHA (Remel ...
-
bioRxiv - Cancer Biology 2022Quote: ... the selected monocytes were cultured in complete Advanced-RMPI 1640 supplemented with either 20 ng/mL human GM-CSF (PeproTech, RockyHill, NJ), for M1 differentiation ...
-
bioRxiv - Immunology 2022Quote: ... OT-I T cells were activated with 10-8 SIINFEKL or SIITFEKL (Cambridge Peptides) in the presence or absence of recombinant human TGFβ (Peprotech, 5ng/ml). Where indicated ...
-
bioRxiv - Immunology 2020Quote: ... cells were washed and cultured for a further 4 days in media containing 20 ng/mL recombinant human IL-2 (Peprotech, London, UK). For analysis of effector cytokine production ...
-
bioRxiv - Cancer Biology 2020Quote: ... H1975 cells were cultured in stem cell culture media (Advanced DMEM/F12 [Thermo Fisher Scientific], 1% Glutamax, B-27 supplement [Thermo Fisher Scientific], 10 ng/mL recombinant human HB-EGF [PeproTech, Cranbury ...
-
bioRxiv - Pathology 2020Quote: ... HUVEC were plated at a density of 10,000 cells/96-well plate in EGM-2 supplemented with recombinant human TNF-α (300-01A, PeproTech EC Ltd), IL1-β (200-01B ...
-
bioRxiv - Developmental Biology 2021Quote: ... LCDMYI supplementation was added as indicated at the following concentrations: 10 ng ml−1 recombinant human LIF (L, 10 ng ml−1; Peprotech, 300-05), CHIR99021 (C ...
-
bioRxiv - Immunology 2020Quote: ... Alternately M2 MΦs were activated as follows: M2a MΦs from stimulation with 20 ng/mL recombinant human IL -4 (PeproTech; Rock Hill, NJ, USA) and 25 ng/mL recombinant human IL-13 (PeproTech ...
-
bioRxiv - Biochemistry 2022Quote: ... cells were diluted to a density of 6e4/mL and 50 μL (3e3 cells) were added to 50 μL of media containing 2x of a serial dilution of human CSF3 (PeproTech 300-23) in a 96-well plate ...
-
bioRxiv - Bioengineering 2022Quote: ... Hydrogels were cultured in 48 well plates for 7 days and maintained in ECM with or without additional growth factors (± 100 ng/mL recombinant human VEGF165; PeproTech 100-20) and hormones ...
-
bioRxiv - Neuroscience 2022Quote: ... hNPCs were plated on poly-L-ornithine- and laminin-coated plates and were differentiated with growth media (without FGF-b) supplemented with 25ng/mL recombinant human glial-derived neurotrophic factor (GDNF; PeproTech US, NJ). Fifty percent of cell media were removed every three to four days and replaced with fresh GDNF supplemented media ...
-
bioRxiv - Immunology 2022Quote: ... Specificity was validated by performing normalized supernatant ELISAs against the extracellular domain of human TNFR2 (Peprotech, 310-12 and in-house production). Flow cytometry phenotyping of hybridoma cells was performed on a BD FACS Aria III ...
-
bioRxiv - Neuroscience 2024Quote: ... Acute treatment was evaluated in 9 month-old mice by pre-mixing and co-administering 5 µg HCT-555 with 25 ng recombinant human BDNF (Peprotech, 450-02) into one tibialis anterior muscle and 5 µg HCT-555 with PBS vehicle into the contralateral tibialis anterior ...
-
bioRxiv - Immunology 2024Quote: HIOs cultured for 7-12 days were treated with 0.1-10 µM indicated JAK inhibitor 1h prior to 50ng/ml human recombinant IFN-γ (300-02, PeproTech, Rehovot, Israel) stimuli for 24h ...
-
bioRxiv - Biochemistry 2024Quote: ... Medium was replaced 24 h later with DMEM-10% FCS supplemented or not with 50 ng/ml human IL-1β (PeproTech, 200-01B). Luciferase activity was measured 5 h later using Bright-Glo Luciferase Assay System (Promega ...
-
bioRxiv - Cancer Biology 2024Quote: ... After inhibitor treatment spheroids were treated with a combination of growth factors or vehicle for 30 minutes before fixation in-situ: 100 nM Human IGF1 (Peprotech #100-11) and 100 nM Human EGF (Peprotech #AF-100-15-1mg) ...
-
bioRxiv - Molecular Biology 2022Quote: ... Cytokine stimulation of human articular chondrocytes was performed on cells at confluence by adding 10ng/ml interleukin-1β (IL-1β, Peprotech, London UK) to standard media and stimulating cells for up to 6 hours.
-
bioRxiv - Immunology 2023Quote: All cell lines were seeded in flat 96-well plates at a density of 0.25 × 105 cells/well and cultured for 24 h in the absence or presence of human recombinant IFNγ and TNFα with activities of 2 × 107 Units/mg (PeproTech, Rehovot, Israel). Cells lines were then co-cultured with 100,000 (at density of 1×106 cell/ml ...
-
bioRxiv - Cell Biology 2023Quote: ... The medium was changed every day until day 8 when medium was replaced by E6 medium supplemented with 10 ng/mL human Platelet-Derived Growth Factor BB (PDGF-BB, PeproTech, 100-14B) and 2 ng/mL Recombinant Human Transforming Growth Factor-β1 (TGF-β1 ...
-
bioRxiv - Immunology 2023Quote: ... 10 million cells were resuspended in 10 ml medium and stimulated with 25ng/ml recombinant human interleukin-2 (IL-2) (Peprotech #AF200-02). Vγ9+Vδ2+ T cells were kept overnight at 37°C and 5% CO2.
-
bioRxiv - Bioengineering 2023Quote: ... M1-like MØs were obtained by culturing M0 MØs in RPMI media supplemented with 10pg/ml Lipopolysaccharides (MilliporeSigma, MO, USA) and 1 ng/ml human Interferon-gamma (Peprotech, NJ, USA). Protocol for generating M0 ...
-
bioRxiv - Biochemistry 2024Quote: ... Sigma-Aldrich P6407) in OPC proliferation media (DMEM-Sato) supplemented with 10 ng/ml recombinant human ciliary neurotropic factor (CNTF; Peprotech 450-13), 10 µM forskolin (Sigma-Aldrich F6886) ...
-
bioRxiv - Microbiology 2024Quote: THP-1 macrophages were seeded on glass coverslips in a 24-well plate (5×105 cells/well) and were activated by 10ng/ml of human IFN-γ (Peprotech, USA) for 16 hours before infection ...
-
bioRxiv - Neuroscience 2024Quote: ... one well of dissRO and iMG-dissRO were treated with recombinant human CCL2 (C-C Motif Chemokine Ligand 2, Peprotech, #300-04) at a final concentration of 10 ng/mL ...
-
bioRxiv - Cancer Biology 2024Quote: ... Exploration of BMP2 effect on HSJD-DIPG-012 and HSJD-DIPG-014 spheroids was done using 10 ng/mL of human recombinant BMP2 (Peprotech, 120-02C) before protein quantification by western blot.
-
bioRxiv - Developmental Biology 2024Quote: ... They were resuspended in EB culture media supplemented with final concentrations of 100 ng/mL human stem cell factor (hSCF, Peprotech #300-07), 100 ng/mL fms-like tyrosine kinase 3 ligand (hFLT3L ...
-
bioRxiv - Bioengineering 2024Quote: ... in T75 flasks at 2 million/mL and differentiated for 7-9 days with 50 ng/mL recombinant human M-CSF (300-25, PeproTech, Cranbury, NJ) and 20 ng/mL recombinant human IL-4 (200-04 ...
-
bioRxiv - Biochemistry 2024Quote: ... the media was then exchanged for 2ml/well of mesoderm induction media (MIM), composed of STEMdiff APEL 2 Medium (STEMCELL, 05275) + 50ng/ml Recombinant Human FGF-basic (Peprotech, 100-18B), 50ng/ml Human BMP-4 (E.coli derived ...
-
bioRxiv - Developmental Biology 2021Quote: DE-to-GT (3 d): DE intermediates were incubated in basal medium-I supplemented with 50 ng/ml FGF7 (PeproTech) for 3 days.
-
bioRxiv - Neuroscience 2021Quote: ... hiPSC-derived astrocytes were treated with vehicle control or IL-1α (3 ng/mL; Peprotech cat. no. AF-200-01A), TNF (30 ng/mL ...
-
bioRxiv - Developmental Biology 2020Quote: ... and cytokines (100 ng/mL SCF, 100 ng/mL IL-3 and 100 ng/mL Flt3 ligand, all from PeproTech). After 7 days of co-culture ...
-
bioRxiv - Neuroscience 2021Quote: ... 1% penicillin/streptomycin) was changed every 3-5 days and M-CSF was supplemented (Peprotech 315-02, 5 ng/mL) for 2 weeks ...
-
bioRxiv - Neuroscience 2021Quote: ... Pre-osteoclasts were obtained after 3 days of stimulation with macrophage colony-stimulating factor (M-CSF 30 ng/mL, PeproTech). Adherent cells were then detached and seeded on top of bone slices (boneslices.com ...
-
bioRxiv - Cancer Biology 2020Quote: ... Ba/F3 cells and Ba/F7 cells were maintained in medium supplemented with 10 ng/ml recombinant mouse interleukin 3 (IL3) and interleukin 7 (IL7) (PeproTech), respectively ...
-
Combining LSD1 and JAK-STAT inhibition targets Down syndrome-associated myeloid leukemia at its corebioRxiv - Cancer Biology 2022Quote: ... and a cytokine cocktail (FLT3-L, SCF, IL-6, IL-3, TPO; all purchased from PeproTech, Rocky Hill, NJ, USA). Samples were taken from pediatric AML patients treated as part of the AML Berlin-Frankfurt-Münster study group ...
-
bioRxiv - Cancer Biology 2022Quote: ... 10 ng/mL IL-3 (Cat# 200-03) and 10 ng/mL G-CSF (Cat# 300-23; all cytokines from Peprotech), 100 IU/mL penicillin ...
-
bioRxiv - Bioengineering 2022Quote: ... 0.1% BSA Fraction V [Gemini Bio]) supplemented with CK+DCIR additives (3 μM CHIR99021 [CHIR; PeproTech], 10 ng/mL keratinocyte growth factor [KGF; PeproTech] ...
-
bioRxiv - Molecular Biology 2022Quote: ... supplemented with cytokines (150 ng/ml SCF, PeproTech, Cat 300-07; 150 ng/ml Flt-3, PeproTech, Cat 300-19; 10 ng/ml IL-6, PeproTech, Cat 200-06 ...
-
bioRxiv - Molecular Biology 2022Quote: ... 150 ng/ml Flt-3, PeproTech, Cat 300-19; 10 ng/ml IL-6, PeproTech, Cat 200-06; 25 ng/ml G-CSF, PeproTech Cat 300-23 ...
-
bioRxiv - Cancer Biology 2020Quote: ... 100 ng/mL fms-like tyrosine kinase 3 ligand (Flt-3L) and 50 ng/mL thrombopoietin (TPO) (all from Peprotech). ES and IPS derived EB were cultured in ultra low attachment 6-well plates (Costar ...
-
bioRxiv - Molecular Biology 2021Quote: ... cells were dissociated and 3×106 cells were plated in a bacterial petri dish in N2B27 medium supplemented with EGF 10 ng/mL (Peprotech) and FGF2 10 ng/mL (R&D systems) ...
-
bioRxiv - Cancer Biology 2022Quote: ... 100 ng/mL fms-like tyrosine kinase 3 ligand (Flt-3L) and 50 ng/mL thrombopoietin (TPO) (all from Peprotech). ES and IPS derived EB were cultured in ultra-low attachment 6-well plates (Costar ...
-
bioRxiv - Cell Biology 2022Quote: ... The monolayer was washed 3 times with HBSS to remove non-adherent cells and monocytes were differentiated into macrophages for 3 days in RPMI media + 10% FBS containing 30 ng/ml M-CSF (PeproTech) and for further 4 days in media containing 30 ng/ml RANKL (PeproTech ...
-
bioRxiv - Developmental Biology 2022Quote: ... 150 μl of N2B27 medium supplemented with 100 ng/ml Activin A (QKine, Cambridge, UK) and 3 μM chiron (Peprotech) was added to each well ...
-
bioRxiv - Immunology 2024Quote: ... Cells were expanded for at least 1 week in the presence of IL-3 and 30 ng/mL recombinant SCF (Peprotech) to obtain peritoneal cavity-derived mast cells (PCMCs) ...
-
bioRxiv - Cancer Biology 2024Quote: ... containing recombinant mouse cytokines (20 ng/ml IL-3, 50 ng/ml IL-6, 10 ng/ml Flt3L and 50 ng/ml SCF, PeproTech), 2 mM L-glutamine ...
-
bioRxiv - Bioengineering 2023Quote: ... One half was plated in SCGM medium supplemented with cytokines and 10ng/ml IL-3 (PeproTech, Rocky Hill, NJ, USA) as +IL-3 treatment ...
-
bioRxiv - Neuroscience 2023Quote: ... and plated onto 3 wells of a 6-well plate precoated with GFR Matrigel in N2B27 with 100ng/ml FGFb (100-18B, Peprotech) and 100ng/ml EGF (AF-100-15 ...
-
bioRxiv - Cancer Biology 2023Quote: ... HSCL or fetal liver LSK cells were cultured for 2 weeks on OP9 or OP9-DL4 cell layer in HSCL medium supplemented with 10 ng/mL Flt-3 (PeproTech) and 10 ng/mL IL-7 (PeproTech).
-
bioRxiv - Immunology 2023Quote: ... Cells were cultured with stem cell factor (SCF) and FMS-like tyrosine kinase 3 ligand (FLT3L) (Peprotech, Rocky Hill, USA) for the first 4 days ...