Labshake search
Citations for Peprotech :
1851 - 1900 of 1976 citations for BN80933 CAS 214348 10 4 98% since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... Cells were resuspended in complete RMPI (RPMI supplemented with 10% v/v FBS, 100 penicillin, 100 streptomycin) with 50U of IL-2 (PeproTech) and CD3/CD28 Human T-activator Dynabeads (Thermo Fisher ...
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bioRxiv - Cancer Biology 2023Quote: ... anti-IL-18 (1 µg/mL, MBL), mIgG1 (1 or 10 µg/mL, R&D) antibodies or IL-1RA (100 ng/mL, Peprotech).
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bioRxiv - Cancer Biology 2023Quote: ... HSCL or fetal liver LSK cells were cultured for 2 weeks on OP9 or OP9-DL4 cell layer in HSCL medium supplemented with 10 ng/mL Flt-3 (PeproTech) and 10 ng/mL IL-7 (PeproTech).
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bioRxiv - Immunology 2023Quote: ... the cells were incubated in standard 10% RPMI for Jurkat cells and cytokine enriched 10% RPMI (5 ng/ml IL7 and 5 ng/ml IL15, both from Peprotech) for primary T cells for the duration of experiment ...
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bioRxiv - Genetics 2023Quote: ... and seeded on a poly-D-lysine/laminin plate with N2B27 media supplemented with BDNF and GDNF (10 ng/ml each; Peprotech). Media were changed the next day to facilitate neuronal maturation and survival ...
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bioRxiv - Developmental Biology 2023Quote: ... were cultured up to passage 6 in M199 medium supplemented with 10% FBS and growth factors (PeproTech, EGF and FGF). For ELN silencing ...
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bioRxiv - Cell Biology 2023Quote: ... Bone marrow-derived dendritic cells were generated by culturing bone marrow progenitors in RPMI medium supplemented with 10% FBS and 20ng/ml granulocyte-macrophage colony-stimulating factor (GM-SCF, PeproTech) for 10 days.
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bioRxiv - Immunology 2023Quote: ... HeLa-sia) at a ratio of 1:100 in complete RPMI medium supplemented with 10 ng/mL GM-CSF (PeproTech). Cancer cells were seeded at an initial concentration of 1×10e4 cells/mL and the same amount of medium supplemented with GM-CSF was added at day 4 of the experiment ...
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bioRxiv - Cancer Biology 2023Quote: ... and conditioned media containing approximately 100 ng/ml SCF for the generation of neutrophil-biased cells or GM-CSF 10 ng/ml (Peprotech) for the generation of macrophage progenitors ...
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bioRxiv - Genomics 2023Quote: ... and 3 x 106 cells were plated to grow in suspension in 90 mm bacterial petri dishes containing N2B27 media supplemented with 1 μg/ml doxycycline and 10 ng/ml EGF and FGF (Peprotech). Embryoid-body-like aggregates were collected by mild centrifugation (100 g for 2 min ...
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bioRxiv - Immunology 2023Quote: ... BM cells isolated from WT C57BL/6 mice and Tollip−/− mice were cultured in complete RPMI 1640 medium supplemented with 10 ng/mL M-CSF (Peprotech) in the presence of 1 mM 4-PBA (MilliporeSigma) ...
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bioRxiv - Neuroscience 2023Quote: ... Astrocytes were then cultured with and without 14 x 103 LNCs/well and treated with 10 ng/mL recombinant murine IFNγ (Peprotech), 100 nM PD-1/PD-L1 inhibitor (Thomas Scientific ...
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bioRxiv - Neuroscience 2024Quote: ... and replated at 50000-75000 cells/cm2 in a poly-L-ornithin-laminin coated 6-well plate with neurobasal medium supplemented with 10 ng/ml PDGFaa (Peprotech), 10 ng/ml IGF1 (Peprotech) ...
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bioRxiv - Cell Biology 2024Quote: ... were routinely cultured on Matrigel-coated plates in hESC culture medium MEF-CM supplemented with 10 ng/ml of bFGF (PeproTech) - with daily medium refreshment ...
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bioRxiv - Cell Biology 2024Quote: ... spheres were dissociated with Accutase and replated on Matrigel-coated plates in ADM supplemented with 10 ng/ml CNTF (PeproTech) and 10 ng/ml BMP-4 for at least 7 days.
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bioRxiv - Cell Biology 2024Quote: ... medium was switched to N2B27 medium supplemented with 10 ng/ml PDGF-BB and 2 ng/ml TGFβ-3 (both from PeproTech) for two days to induce differentiation toward pericyte-like cells ...
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bioRxiv - Cell Biology 2024Quote: ... for 3 days and then cultured in 10% FBS α-MEM media supplemented with 100ng/mL RANKL (PeproTech, 315-11) and 30 ng/mL M-CSF for 6 to 7 days ...
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bioRxiv - Genetics 2024Quote: ... UK)] plus 1000 U/ml Leukemia inhibitor factor (LIF) (Preprotech, UK) and 10 ng/ml basic fibroblast growth factor (bFGF) (Peprotech). iPSC media was replaced every other day until iPSC colonies began to appear and reached a large enough size to manually pick selected colonies ...
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bioRxiv - Immunology 2024Quote: ... PBMCs were thawed at 37°C and incubated in complete RPMI media with 10% heat-inactivated FBS and supplemented with IL-2 (50 U/ml; PeproTech). 1 x 104 PBMCs were plated per well in round bottom clear plate (Greiner ...
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bioRxiv - Immunology 2024Quote: ... Samples were stimulated and incubated overnight in complete RPMI media with 10% heat-inactivated FBS and supplemented with IL-2 (50 U/ml; PeproTech).
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bioRxiv - Molecular Biology 2024Quote: ... Neuronal cultures were maintained in neuronal medium (Neurobasal/B27 medium supplemented with 20 ng/ml BDNF, 10 ng/ml GDNF, both from PeproTech, and 200 ng/ml l-ascorbic acid ...
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bioRxiv - Immunology 2024Quote: ... Reprogramming under immunosuppressive cytokine conditions was performed using DMEM complete medium supplemented with human IL-6 (10-40 ng/ml; Peprotech), TGF-ϕ3 (50-200 ng/ml ...
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bioRxiv - Immunology 2024Quote: Naïve and activated CD4+ T cells (via 72h incubation with αCD3/28 beads) were either left untreated or stimulated with IL-10 (40 μg/ml, PeproTech) for 20 min and then lysed with RIPA buffer containing proteases and phosphatases inhibitors (MilliporeSigma) ...
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bioRxiv - Immunology 2024Quote: ... filtered and cultured for 7 days in complete RPMI medium (RPMI-1640 medium containing L-glutamine plus 10% fetal calf serum) containing 20ng/mL recombinant GM-CSF (Peprotech).
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bioRxiv - Immunology 2024Quote: ... 100 μg/ml streptomycin and 100 units/ml penicillin) and stimulated with plate-bound anti-CD3/CD28 in the presence of 10 ng/ml IL-2 (Peprotech) as indicated ...
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bioRxiv - Molecular Biology 2024Quote: ... The NSCs were maintained in Neural Expansion Media (NEM) composed of NBM supplemented with FGF (10 ng/ml) (100-18C, Peprotech) and EGF (10 ng/ml ...
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bioRxiv - Immunology 2024Quote: ... Siglec-Fhi and Siglec-Flow neutrophils were prepared by culturing bone marrow neutrophils for 2 days in the presence or absence of 10 ng/ml GM-CSF (Peprotech) and 10 ng/ml IL-4 (R&D Systems) ...
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bioRxiv - Immunology 2024Quote: ... 8X106 cells were seeded in 6 mL of DMEM containing 10% FBS and 50 ng/mL MCSF (PeproTech #315-02) in a 100mm plate on day 0 ...
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bioRxiv - Immunology 2024Quote: ... Every 48 hours the cells were centrifuged and the pellet was resuspended between 1 and 2 × 106 per mL in fresh complete media containing the OVA peptide 3 μg/mL and IL-2 (100 U/mL) after the second stimulation IL-15 (10 ng/mL, Peprotech) was added The number of stimulations is indicated in the figure/text ...
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bioRxiv - Physiology 2024Quote: ... The differentiated endoderm cells were further treated with RPMI 1640/B27 Minus Insulin along with 10 ng/ml basic fibroblast growth factor (FGF) (Cat 3100-18B, Peprotech) and 20 ng/ml Bone morphogenic factor 4 (BMP4 ...
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bioRxiv - Immunology 2024Quote: ... BMDC and neutrophils were generated in 7-day cultures with complete RPMI-10 supplemented with 15ng/mL recombinant GM-CSF (PeproTech). Cells from the floating fraction were harvested and BMDC were sorted using anti-CD11c MicroBeads (Miltenyi Biotech ...
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bioRxiv - Immunology 2024Quote: ... splenocytes were adjusted to a concentration of 3 x 106 cells/mL in T cell medium containing 10 ng/mL rhIL-7 (Peprotech) and 2 µg/mL anti-mouse CD28 (clone 37.51 ...
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bioRxiv - Immunology 2024Quote: ... T cells were removed from stimulation and cultured in complete medium supplemented with 10 ng/ml recombinant mouse IL-7 (Peprotech) for 3-5 days as previously described (39) ...
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bioRxiv - Immunology 2024Quote: ... or unstained PBMCs were seeded at low density on a plasma-treated chip containing cIMDM supplemented with 10 ng/ml IL-15 (PeproTech) and 100 U/ml IL-2 (PeproTech) ...
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bioRxiv - Developmental Biology 2024Quote: ... coated plates in AFX medium which consisted of N2B27 supplemented with 10 ng/mL human/mouse/rat recombinant ACTIVIN A (Peprotech), 12 ng/mL FGF2 (Cambridge Stem Cell Institute ...
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bioRxiv - Bioengineering 2024Quote: ... small molecules and cytokines were added in the basal medium at the following final concentrations: 10 ng/mL FGF2 (F; Peprotech), 10 ng/mL Activin-A (A ...
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bioRxiv - Cancer Biology 2024Quote: ... To differentiate bone marrow cells to macrophages the cells were plated in Phenol Red Free-DMEM media supplemented with 10% heat-inactivated charcoal-stripped serum in the presence of 30 ng/ml MCSF (Cat# 312-02, PeproTech) and 1% Pen/Strep ...
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bioRxiv - Cancer Biology 2024Quote: ... and then maintained throughout the protocol by replacing the medium (including dox) with 10 ng/ml human basic-FGF (Peprotech) every 2 days ...
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bioRxiv - Cancer Biology 2024Quote: ... cells were detached with 0.1 mM EDTA (Life Sciences) and re-plated with media supplemented with 10 μM Y-27632 Dihydrochloride (PeproTech, #1293823). CHLA-01-Med primary and CHLA-01R-Med recurrent MB cell lines purchased from ATCC were cultured in suspension in Gibco DMEM-F12 media (Life Technologies ...
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bioRxiv - Developmental Biology 2024Quote: ... The tenogenic differentiation medium contained a culture medium supplemented with 10 ng/mL TGF-β1 (Peprotech, Rocky Hill, NJ, USA), 10 ng/mL GDF-5 (R&D Systems ...
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bioRxiv - Immunology 2021Quote: ... when the cells were stimulated with 1000 U/ml of rhIL-2 and the combination of 10 ng/ml of recombinant murine (rm) IL-12 (210-12, PeproTech Inc) and 40 ng/ml of rm IL-15 (210-15 ...
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bioRxiv - Developmental Biology 2020Quote: ... DFs cultured in DMEM/F12 with 2% FBS supplemented with TGFβ1 or TGFβ3 (10 ng/ml; PeproTech EC, Ltd., London, UK) or 5 mM citric acid (diluent for lyophilized TGFNβ ...
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bioRxiv - Neuroscience 2022Quote: ... Electroporated cells were transferred to two GelTrex coated 100 mm dishes (1K and 10K) in mTeSR medium supplemented with 10 uM ROCK inhibitor (PeproTech, 1293823) and placed into CO2 incubator for 2 days ...
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bioRxiv - Cell Biology 2021Quote: ... PMNs (2.5×106 cells/ml) were used as a control or stimulated with 10 ng/ml TNFα (PeproTech #AF-300-01A) for 15 min at 37°C ...
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bioRxiv - Cell Biology 2021Quote: ... penicillin/streptomycin (50 IU/mL penicillin and 50 μg/mL streptomycin) and 10 pg/μL recombinant murine macrophage colony stimulating factor (PeproTech, Inc.).
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bioRxiv - Cell Biology 2022Quote: HUVECs were transfected 48 h before the assay and transferred to gelatin-coated black-walled 96-well plates (Falcon) and stimulated with TNF-α (10 ng/ml; Peprotech) or PBS (Gibco ...
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bioRxiv - Neuroscience 2020Quote: Inflammation in primary glial cultures was induced by the addition of proinflammatory agents such as interleukin 1 beta (IL1β, 10 ng/mL; PeproTech, France) or lipopolysaccharide (LPS ...
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bioRxiv - Immunology 2024Quote: ... A single round of co-sedimentation with retroviral stock was performed in medium containing 5% WEHI-3B-conditioned medium and 10 ng/mL IL-7 (Peprotech, USA). After transduction ...
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bioRxiv - Cancer Biology 2024Quote: ... 5 x 105 splenocytes from single cell suspensions were incubated with increasing concentrations of recombinant mouse IFNγ (0, 0.1, 1, 10, 100 ng/mL) for 48 hours (Peprotech, 315-05). Cells were then harvested and incubated with a panel of antibodies containing anti-mouse CD19 (Biolegend,115504) ...
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bioRxiv - Molecular Biology 2023Quote: ... in the absence or presence of 10 ng/mL IL-1β (FujiFilm Wako Pure Chemical) and 20 ng/mL TNF-α (PeproTech). Differentiated astrocytes expressing ERT2-E2F1-1′C were prepared from NPCs that were previously infected with retrovirus encoding ERT2-E2F1-1′C followed by selection with 0.25 μg/mL puromycin (Nacalai tesque) ...