Labshake search
Citations for Peprotech :
1851 - 1900 of 1916 citations for 7 Butyl 8 mercapto 1 3 dimethyl 3 7 dihydro purine 2 6 dione since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... The following anti-mouse primary antibodies were used: anti-RELM-beta (1:500, rabbit polyclonal antibody, Peprotech, 500-p215), anti-MUC2 (1:200 ...
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bioRxiv - Cancer Biology 2024Quote: ... Growth factors were also given to media surrounding basement membrane extract (Cultrex, Biotechne): 1% Mouse recombinant Noggin (Peprotech, UK), 1% mouse recombinant EGF (Invitrogen ...
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bioRxiv - Molecular Biology 2023Quote: ... and resuspended in differentiation medium I-10 + M-CSF (i.e., IMDM, 10 % FBS, 1 mg/ml Pen/Strep containing 25 ng/ml recombinant murine M-CSF (PeproTech)) at a density of 1-2 Mio ...
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bioRxiv - Neuroscience 2023Quote: ... and 100 ng/mL of CX3CL1 (Fractalkine/ chemokine (C-X3-C motif) ligand 1) (Peprotech, Catalog No. 300-31) for up to 12 days.
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bioRxiv - Neuroscience 2024Quote: ... The NB-B27-S included human ciliary neurotrophic factor (CNTF, 10 ng/mL, 1:1000, Peprotech, AF-450-13), glial-derived neurotrophic factor (GDNF ...
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bioRxiv - Cancer Biology 2021Quote: ... SNKO-1 cells were obtained from DSMZ and cultured in RPMI with 10% FSB and 10 ng/ml GM-CSF (PeproTech). EOL-1 cells were obtained from DSMZ and cultured in RPMI with 10% FBS ...
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bioRxiv - Immunology 2021Quote: ... and cultured at 1-2 × 106/ml in 12-well plates with 1 ml of differentiation medium containing 50 ng/ml interleukin 4 (IL-4, 200–04 A, Peprotech) and 100 ng/ml granulocyte-monocyte colony stimulating factor (GM-CSF ...
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bioRxiv - Neuroscience 2021Quote: ... once daily for 14 days starting at P30 with either vehicle (saline) or rhIGF-1 (2.5 mg/kg; Peprotech, USA) dissolved in saline with 0.01% Bovine Serum Albumin (weight/volume).
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bioRxiv - Molecular Biology 2020Quote: ... supplemented with 1% HSA (Baxter) and cytokines (SCF 100ng/mL, FLT3L 100ng/mL and TPO 100ng/mL; all from PeproTech)55 ...
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bioRxiv - Immunology 2019Quote: Bone marrow-derived macrophages (BMDMs) were cultured in complete RPMI with recombinant human CSF-1 (50 ng/ml; PeproTech Inc.) for 6 days ...
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bioRxiv - Neuroscience 2020Quote: ... The final pellets were dissociated with pre-warmed 10% fetal bovine serum in DMEM supplemented with 20 ng mL-1 mouse M-CSF (PeproTech) and plated in culture dishes ...
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bioRxiv - Cancer Biology 2021Quote: ... Cells were allowed to attach on coverslip for 1 h and then cells were incubated with murine recombinant CCL2 (Peprotech) for 5 h ...
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bioRxiv - Bioengineering 2021Quote: Lyophilized 90 % PEGDMA microrods were loaded by resuspending ∼100,000 (100 k) microrod aliquots in 20 μL of 1 mg/mL recombinant human β-NGF (Peprotech) as described in the Protein loading of PEGDMA microrods section ...
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bioRxiv - Bioengineering 2021Quote: ... the organoids were transferred from the aforementioned PDMS microwells to a previously published spinning bioreactor19 with a speed of 80 revolutions per minute in E6 medium containing 10 ng mL−1 FGF2 (Peprotech), 20 ng mL−1 BDNF (Peprotech) ...
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bioRxiv - Immunology 2021Quote: The differentiation of isolated monocytes into macrophages was performed in RPMI 1640 GlutaMAx medium with 10% FCS and 1% P/S containing 100 ng/mL macrophage colony-stimulating factor (M-CSF) (PeproTech) for 7 days ...
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bioRxiv - Immunology 2021Quote: ... Activated C7 cells were concentrated and 4 ml of culture was resuspended in 1 ml RPMI+ containing 10 μg/mL ESAT61-20 peptide + 20 ng/mL IL-12 (Peprotech) + 20 ng/mL IL-2 (Peprotech) ...
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The skin environment controls local dendritic cell differentiation and function through innate IL-13bioRxiv - Immunology 2021Quote: ... were harvested and one million cells were resuspended in 1 mL of TCM in 24 wells plates and stimulated with recombinant mouse IL-13 (Peprotech) at a concentration of 100 ng/mL for 30 min at 37°C ...
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bioRxiv - Immunology 2020Quote: ... the obtained bone marrow cells were cultured in RPMI-1640 containing 10% FBS and 10 ng mL−1 mouse macrophage colony-stimulating factor (M-CSF, Peprotech) in a humidified 5% CO2 atmosphere at 37 °C for six days ...
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bioRxiv - Developmental Biology 2024Quote: ... cells were replated as single cells onto poly-D-lysine-laminin-coated plates at 1×106 cells/cm2 for astrogenic switch and progenitor expansion in N2B27 media supplemented with 10 ng/mL FGF2 (Peprotech) and 10 ng/mL Human EGF (Peprotech ...
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bioRxiv - Immunology 2024Quote: ... PBL, #12400-1; 200U IL-1α #211-11A, 200U IL-1β #211-11Β or both together at 200U total, PeproTech) to allow for pulmonary delivery one week (unless otherwise stated in the figure legends ...
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bioRxiv - Genomics 2023Quote: ... and 3 x 106 cells were plated to grow in suspension in 90 mm bacterial petri dishes containing N2B27 media supplemented with 1 μg/ml doxycycline and 10 ng/ml EGF and FGF (Peprotech). Embryoid-body-like aggregates were collected by mild centrifugation (100 g for 2 min ...
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bioRxiv - Cell Biology 2023Quote: ... cells were either given fresh MC medium or cultured in basal MC medium (1% FBS) or in basal MC medium supplemented with 50ng/mL recombinant human VEGF-A165 (100-20; Peprotech) and culture for another 3 days.
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bioRxiv - Immunology 2023Quote: ... HeLa-sia) at a ratio of 1:100 in complete RPMI medium supplemented with 10 ng/mL GM-CSF (PeproTech). Cancer cells were seeded at an initial concentration of 1×10e4 cells/mL and the same amount of medium supplemented with GM-CSF was added at day 4 of the experiment ...
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bioRxiv - Neuroscience 2023Quote: ... with the addition of growth factors (10 ng/mL BDNF, Peprotech #450-02; 10 ng/mL GDNF, Peprotech #450-10; 1 ng/mL TGF-β1, Peprotech #100-21C ...
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bioRxiv - Microbiology 2023Quote: ... Monocyte-derived DCs were differentiated by stimulating THP-1 cells with human GMCSF (1500 IU/ml) and IL-4 (1500 IU/ml) (Peprotech) for 5 days with half the medium replaced at day 3 ...
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bioRxiv - Neuroscience 2024Quote: ... 0.1% Tween-20 (TBS-T) and bound SDF-1α-CXCL12 chemokine was detected with anti-murine SDF-1α-CXCL12 antibody (diluted 1:2000, Peprotech) followed by an HRP-conjugated anti-rabbit antibody (diluted 1:16000 ...
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bioRxiv - Immunology 2024Quote: ... PBMC were thawed and recovered in complete RPMI overnight without exogenous cytokines before being stimulated with either 1 ng/mL of IL-15 (Peprotech) or 1 ng/mL of IL-15+ 5 ng/mL TGF-β (Peprotech ...
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bioRxiv - Immunology 2024Quote: ... 3×105 CD14+ monocytes were seeded in a 12-well plate in 1 mL complete medium containing 20 ng/mL GM-CSF (#300-03; Peprotech) or 50 ng/mL M-CSF (#300-25 ...
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bioRxiv - Neuroscience 2020Quote: Inflammation in primary glial cultures was induced by the addition of proinflammatory agents such as interleukin 1 beta (IL1β, 10 ng/mL; PeproTech, France) or lipopolysaccharide (LPS ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: HBD1 and LL-37 peptide release was quantified in supernatants from 25 day long-term cultured A549 cells using a human BD-1 Standard ABTS ELISA Development Kit (PeproTech, Germany) and LL-37 human ELISA kit (HycultBiotech ...
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bioRxiv - Neuroscience 2019Quote: ... with 1% platelet-poor plasma-derived bovine serum (Biomedical Technologies 50-443-029) with 20 ng/mL bFGF (Peprotech 100-18B), and 10µM retinoic acid (RA ...
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bioRxiv - Immunology 2019Quote: ... bone marrow cells were plated at a density of 5×105 cells/mL in RPMI-1640 + 10% FCS + 100 U/mL penicillin/streptomycin + 1 mM β-mercaptoethanol (BM medium) with 20 ng/mL murine GM-CSF (Peprotech) and cultures in a humidified environment at 37°C and 5% CO2 ...
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Combination image flow cytometry reveals novel methods for isolating megakaryocyte progenitors cellsbioRxiv - Cell Biology 2019Quote: ... 1×107 cells were incubated in IMDM + 10% FBS supplemented with 50 ng/ml of recombinant murine TPO (PeproTech, #315-14) in a T75 flask at 37 °C in 5% CO2 for 72 h.
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bioRxiv - Immunology 2020Quote: ... Rinse solution was then aspirated and replaced with 1 mL of 2X concentrated EB medium (1X EB media: OXE8 medium supplemented with 50 ng/mL BMP4 (Peprotech, #PHC9534), 50 ng/mL VEGF (Peprotech ...
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bioRxiv - Cancer Biology 2024Quote: ... 5 x 105 splenocytes from single cell suspensions were incubated with increasing concentrations of recombinant mouse IFNγ (0, 0.1, 1, 10, 100 ng/mL) for 48 hours (Peprotech, 315-05). Cells were then harvested and incubated with a panel of antibodies containing anti-mouse CD19 (Biolegend,115504) ...
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bioRxiv - Neuroscience 2022Quote: ... in DMEM medium (+10 % FCS and 1% P/S) and stimulated with either 10 ng/ml TNF-α and 10 ng/ml IL-1β (both from Peprotech) or vehicle control ...
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bioRxiv - Biochemistry 2023Quote: ... 1% penicillin-streptomycin (P/S)) supplemented with 10-100 ng/mL recombinant murine fibroblast growth factor (Peprotech, Rocky Hill, NJ, USA). At passage 3 ...
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bioRxiv - Bioengineering 2023Quote: ... reprogramming was continued in E8 medium and from Day 6 to Day 18 in E7 medium (E6 medium, cat. A1516401, Thermo Fisher Scientific + 100ng ml-1 bFGF, cat. 100-18B, Peprotech). On Day 18 ...
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bioRxiv - Cancer Biology 2023Quote: ... Colonic organoids were cultured in organoid base medium further supplemented with 100 ng mL−1 murine WNT3A (mWNT3A, Peprotech 315-20), 50 ng mL−1 mEGF (Thermo PMG8041) ...
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bioRxiv - Cell Biology 2022Quote: ... The cell suspension was washed with PBS and plated in DMEM with 10 ng mL-1 M-CSF (Peprotech Inc, 315-02). Following 5 days of culture ...
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bioRxiv - Biochemistry 2019Quote: ... at 1 M cells per well in unconditioned medium with or without priming in 25 U IFN-γ (PeproTech, Rocky Hill, NJ). Bone-marrow-derived mast cells were prepared as described previously (55) ...
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bioRxiv - Bioengineering 2021Quote: ... HepatoSTIM™ medium was supplemented with 10 ng mL−1 of recombinant human TGF-β1 (100-21; PeproTech®, Rocky Hill, NJ), 100 ng mL−1 of recombinant human GDF-15 (120-28C ...
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bioRxiv - Immunology 2022Quote: ... 235 μL of complete DMEM supplemented with 1 % rat serum with or without chemotactic stimuli 30 ng/mL CXCL10 (PeproTech, 400-33) and CCL5 (PeproTech ...
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bioRxiv - Neuroscience 2022Quote: ... 100ng/mL cluster of differentiation 200 (CD200; Novoprotein # C31150UG) and100ng/mL fracktaline chemokine C-X3-C motif ligand 1 (CX3CL1; Peprotech # 300-31) (MBM + 5 GFs) ...
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bioRxiv - Cancer Biology 2020Quote: ... H1975 cells were cultured in stem cell culture media (Advanced DMEM/F12 [Thermo Fisher Scientific], 1% Glutamax, B-27 supplement [Thermo Fisher Scientific], 10 ng/mL recombinant human HB-EGF [PeproTech, Cranbury ...
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bioRxiv - Cancer Biology 2019Quote: ... using 5 μg/mL of the monoclonal anti-mouse IFN-γ antibody (eBioscience) for capture and 1 μg/mL of the polyclonal biotinylated anti-mouse IFN-γ antibody (PeproTech) for detection.
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bioRxiv - Neuroscience 2023Quote: ... L-glutamine and 1% Penicillin-Streptomycin (v/v) supplemented with CHIR-99021 (Stemgent, 0.5 μM) and brain derived neurotrophic factor (BDNF, PeproTech, 20 ng/ml). On the day in vitro 50 ...
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bioRxiv - Cell Biology 2023Quote: ... cells were washed 3 times with PBS and incubated in naïve dedifferentiation medium of mTeSR-1 supplemented with 12 ng/mL bFGF (Peprotech #AF-100-18B), 1 µM PD0325901 (MEKi ...
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bioRxiv - Bioengineering 2023Quote: ... OPCs were cultured using OPC maintenance/proliferation media (OMM) composed of N2B27 supplemented with 10ng/ml insulin-like growth factor (IGF-1; Peprotech, 100-11), 15ng/ml platelet-derived growth factor AA (PDGFaa ...
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bioRxiv - Bioengineering 2023Quote: ... M1-like MØs were obtained by culturing M0 MØs in RPMI media supplemented with 10pg/ml Lipopolysaccharides (MilliporeSigma, MO, USA) and 1 ng/ml human Interferon-gamma (Peprotech, NJ, USA). Protocol for generating M0 ...