Labshake search
Citations for Peprotech :
1801 - 1850 of 2217 citations for IL 3RA Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2024Quote: ... 25 ng/mL recombinant human hepatocyte growth factor (HGF; Peprotech; 100-39), 500 nM A8301 (R&D Systems ...
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bioRxiv - Cell Biology 2024Quote: ... 20 ng/ml recombinant human heregulin β-1 (HRG) (PeproTech, 100-03). Small molecules ...
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bioRxiv - Immunology 2024Quote: ... 50 ng/ml recombinant human M-CSF (PeproTech, Rocky Hill, NJ, USA), 100 U/ml penicillin ...
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bioRxiv - Cell Biology 2020Quote: ... AT2 cells (CD31−CD45−EpCAM+MHCII+) isolated from Krt8-CreERT2;R26RtdTomato were cultured with for 14 days with IL-1β (20ng/ml, Peprotech). Then ...
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bioRxiv - Immunology 2021Quote: ... and cultured at 1-2 × 106/ml in 12-well plates with 1 ml of differentiation medium containing 50 ng/ml interleukin 4 (IL-4, 200–04 A, Peprotech) and 100 ng/ml granulocyte-monocyte colony stimulating factor (GM-CSF ...
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bioRxiv - Immunology 2021Quote: ... for 4 days at 37°C in complete RPMI medium supplemented with 10 ng/mL recombinant mouse IL-5 (PeproTech) and 20 ng/mL IL-2 (402-ML R&D) ...
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bioRxiv - Immunology 2021Quote: ... for 4 days at 37°C in complete RPMI medium supplemented with 10 ng/mL recombinant mouse IL-5 (PeproTech) and 20 ng/mL IL-2 (402-ML R&D) ...
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bioRxiv - Immunology 2021Quote: ... with 2% FBS (HyClone Thermo Fischer Scientific) for 15 minutes with or without murine IL-7 (50 ng/mL, PeproTech). Following stimuli ...
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bioRxiv - Neuroscience 2021Quote: ... hiPSC-derived astrocytes were treated with vehicle control or IL-1α (3 ng/mL; Peprotech cat. no. AF-200-01A), TNF (30 ng/mL ...
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bioRxiv - Immunology 2021Quote: ... cells were removed from the TCR signal and re-cultured by doubling the area and volume in media supplemented with 20 U/ml IL-2 (Peprotech), which was added every 3 days ...
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bioRxiv - Immunology 2021Quote: ... BM cells isolated from BALB/c mice were cultured for 6 days in medium supplemented with granulocyte-macrophage colony-stimulating factor (GM-CSF) and IL-4 (Peprotech) to generate DCs ...
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bioRxiv - Microbiology 2020Quote: ... 20 ng/ml granulocytes-macrophage colony-stimulating factor (GM-CSF) and 5ng/ml Interleukin 4 (IL-4) (both from Peprotech)) for 7 days ...
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bioRxiv - Immunology 2019Quote: ... and were then cultured at 1-2×106/ml in 12 well-plates with differentiation media containing 50 ng/ml Interleukin 4 (IL-4, 200-04 A, Peprotech) and 100 ng/ml granulocyte-monocyte colony stimulating factor (GM-CSF ...
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bioRxiv - Systems Biology 2019Quote: ... All in vitro T cell generation cultures were supplemented with 5ng/mL Flt3-L and 5 ng/mL IL-7 (Peprotech), and were sorted after 6 or 7 total days of culture before transducing with retroviral vectors or treating with small molecule inhibitors ...
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bioRxiv - Developmental Biology 2020Quote: ... and cytokines (100 ng/mL SCF, 100 ng/mL IL-3 and 100 ng/mL Flt3 ligand, all from PeproTech). After 7 days of co-culture ...
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bioRxiv - Bioengineering 2021Quote: ... T cells were cultured at a density of 1 million cells/mL in ImmunoCult-XF T cell Expansion Medium (STEMCELL) supplemented with 200 U/mL IL-2 (Peprotech) and stimulated with ImmunoCult Human CD3/CD28/CD2 T cell Activator (STEMCELL ...
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bioRxiv - Neuroscience 2020Quote: ... 2×105 cells/well/1mL AM medium were seeded on 12-well plates and were treated with 10 ng/ml IL-1β (Peprotech) for 24 hours ...
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bioRxiv - Neuroscience 2022Quote: ... astrocyte cultures at 80% confluence in 6-well plates (in E6 medium containing BMP4 and FGF2) were treated with vehicle control or IL-1a (5 ng/ml; Peprotech), TNFα (10 ng/ml ...
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bioRxiv - Immunology 2022Quote: ... 0.25 µg/mL Amphotericin B and 2 mM L-glutamine and treated with 10 ng/mL recombinant IL-12p70 (Peprotech) for 24 hours for supernatant IFN-γ analysis.
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bioRxiv - Immunology 2022Quote: ... OT-I T cells were maintained inactivated in vitro (‘No TCR’ condition) with recombinant mouse IL-7 (Peprotech, 10ng/ml). For IL-2 replenishment experiments ...
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bioRxiv - Cancer Biology 2022Quote: ... 10 ng/mL IL-3 (Cat# 200-03) and 10 ng/mL G-CSF (Cat# 300-23; all cytokines from Peprotech), 100 IU/mL penicillin ...
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bioRxiv - Bioengineering 2021Quote: ... were isolated from blood of a healthy donors by density-gradient centrifugation and grown in RPMI 1640 medium supplemented with 10% FCS and 1000 U/ml recombinant IL-2 (PeproTech) and activated with 1 μg/ml anti-CD3 and anti-CD28 antibodies ...
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bioRxiv - Molecular Biology 2022Quote: ... supplemented with cytokines (150 ng/ml SCF, PeproTech, Cat 300-07; 150 ng/ml Flt-3, PeproTech, Cat 300-19; 10 ng/ml IL-6, PeproTech, Cat 200-06 ...
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bioRxiv - Molecular Biology 2022Quote: ... 150 ng/ml Flt-3, PeproTech, Cat 300-19; 10 ng/ml IL-6, PeproTech, Cat 200-06; 25 ng/ml G-CSF, PeproTech Cat 300-23 ...
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bioRxiv - Immunology 2022Quote: Splenic T-cells from Ldlr-/- and T-AbcdkoLdlr-/- mice were isolated and stimulated with Mouse T-Activator CD3/CD28 Dynabeads (ratio beads to cells 1:2) in the presence of 100 U/mL IL-2 (Peprotech) and 5 ng/mL TGFβ1 (Peprotech ...
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bioRxiv - Cancer Biology 2019Quote: ... and maintained in culture for a total of approximately 2-3 months in DMEM supplemented with 1 ng/mL IL-3 (Peprotech). Mycoplasma testing was not performed ...
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bioRxiv - Immunology 2021Quote: 750 000 MCs were either FACS-sorted after 24h of coculture with resting or anti-CD3/28 coated beads-activated effector/memory CD4+ T cells or stimulated for 4 hours with 10 ng/mL of IL-33 (Peprotech) or with 100 ng/mL of DNP-BSA (thermo-fischer scientific) ...
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bioRxiv - Immunology 2020Quote: ... in the presence of 50 ng/ml granulocyte-macrophage colony-stimulating factor (GM-CSF) and of 20 ng/ml interleukin-4 (IL-4) (PeproTech). DCs stimulation was performed 6 days post-isolation of monocytes ...
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bioRxiv - Immunology 2021Quote: ... Activated C7 cells were concentrated and 4 ml of culture was resuspended in 1 ml RPMI+ containing 10 μg/mL ESAT61-20 peptide + 20 ng/mL IL-12 (Peprotech) + 20 ng/mL IL-2 (Peprotech) ...
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The skin environment controls local dendritic cell differentiation and function through innate IL-13bioRxiv - Immunology 2021Quote: ... were harvested and one million cells were resuspended in 1 mL of TCM in 24 wells plates and stimulated with recombinant mouse IL-13 (Peprotech) at a concentration of 100 ng/mL for 30 min at 37°C ...
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bioRxiv - Microbiology 2021Quote: ... cells were treated with recombinant mouse IFNβ (1000 U/ml, Stratech) or IL-10 (250 ng/ml, catalogue 210-10 Peprotech) 3 h prior to collection for immunoblotting.
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Neutralizing activity of broadly neutralizing anti-HIV-1 antibodies against primary African isolatesbioRxiv - Immunology 2020Quote: ... A total of 2×106 CD4+ T lymphocytes were activated using anti CD3/CD2/CD28 beads (Miltenyi) and cultured in the presence of 100 U/mL IL-2 (Peprotech) at 37 °C and 5% CO2 ...
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bioRxiv - Cell Biology 2021Quote: ... The fused cells were dispersed in 80 ml of GIT medium (Wako, Japan) supplemented with 1 ng/ml IL-6 (PeproTech) and 1×HAT (Kohjin-Bio) ...
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bioRxiv - Immunology 2022Quote: Primary neuronal cells (DIV14) or oligodendrocytes were stimulated with 100 ng/μl of recombinant murine IL-12p70 (#210-12, Peprotech) for 5 min ...
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bioRxiv - Immunology 2022Quote: ... primary neuronal cells were stimulated with 30 ng/μl or 100 ng/μl of Recombinant Murine IL-12p70 (#210-12, Peprotech) for 18 hours ...
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bioRxiv - Immunology 2022Quote: ... or into bone marrow-derived dendritic cells (BMDC) in cRPMI supplemented with recombinant mouse IL-4 and GM-CSF (Peprotech). Influenza virus strains A/California/04/2009 (H1N1) ...
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bioRxiv - Bioengineering 2022Quote: ... and then for three to five days (plasmablast/plasma cell differentiation) in medium containing 50 ng/mL IL-6 (Peprotech),10 ng/mL IL-15 and 15 ng/mL interferon-α2B (Sigma-Aldrich).
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bioRxiv - Immunology 2022Quote: ... The culture conditions used in this study were as follows: HSP condition – cell cultured with IL-15 and -7 (10 μg/ml, PeproTech); HSP+TCR condition – cells cultured with IL-15 and -7 and activated by anti-CD3 (5 μg/ml ...
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bioRxiv - Immunology 2022Quote: ... TCR condition – cells activated with anti-CD3/CD28 antibodies on day 0 and cultured with IL-2 (2 ng/ml, PeproTech); TCR+TCR condition – cells activated with anti-CD3/CD28 on day 0 ...
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bioRxiv - Immunology 2022Quote: ... Th2 cells were generated by activating naïve CD4+ T cells with 1 μg/mL anti-CD3ε/3 μg/mL anti-CD28 for 3 days in complete T cell medium supplemented with 250U recombinant IL-4 (PeproTech), 6 μg/mL anti-IL-12 (BioXcell) ...
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bioRxiv - Immunology 2022Quote: ... splenocytes from control and Ncr1cre Mycfl/fl mice were left unstimulated or stimulated with 50 ng/mL recombinant mouse IL-15 (PeproTech) for 40 min at 37 °C and analyzed for intracellular protein phosphorylation by flow cytometry ...
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bioRxiv - Immunology 2023Quote: ... Isolated T cells were otherwise activated using plate bound anti-CD3 (5 μg/ml, inVIVOMAb Cat# BE0002) in complete medium added with 100 U/ml IL-2 (Peprotech) and soluble αCD28 (0.5 μg/ml ...
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bioRxiv - Immunology 2023Quote: Isolated CD8+ T cells from OT-I mice were maintained in the naive status after CRISPR-Cas9 gene targeting by resting them overnight in complete medium added with IL-7 (Peprotech) at 10 ng/ml ...
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bioRxiv - Immunology 2023Quote: ... 4-6 x 106 P14 CD8 T cells were plated in 6 well plates and pre-cultured in IL-7 (10ng/ml, PeproTech) at 37°C ...
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bioRxiv - Immunology 2023Quote: ... and light zone cells (CD19+GL7+FAS+CXCR4-CD86+) and cultured in the presence of 200 ng/ml Il-9 (PeproTech) in RPMI media for 3 h at 37°C ...
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bioRxiv - Bioengineering 2023Quote: ... One half was plated in SCGM medium supplemented with cytokines and 10ng/ml IL-3 (PeproTech, Rocky Hill, NJ, USA) as +IL-3 treatment ...
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bioRxiv - Immunology 2023Quote: ... CD39+ γδ IELs were treated with 100 μM POM-1 (Biotechne) for 2 h prior to the addition of 10 μM ATP (ThermoScientific) and IL-2 (6 ng/ml, Peprotech). Supernatants were collected and stored at -20°C for future use ...
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bioRxiv - Immunology 2023Quote: ... Cells were resuspended in complete RMPI (RPMI supplemented with 10% v/v FBS, 100 penicillin, 100 streptomycin) with 50U of IL-2 (PeproTech) and CD3/CD28 Human T-activator Dynabeads (Thermo Fisher ...
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bioRxiv - Cancer Biology 2023Quote: ... anti-IL-18 (1 µg/mL, MBL), mIgG1 (1 or 10 µg/mL, R&D) antibodies or IL-1RA (100 ng/mL, Peprotech).
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bioRxiv - Immunology 2023Quote: ... The cells were washed and replated in complete RPMI 1640 medium with 100 U/ml IL-2 (PeproTech, 200-02) 48 hours later ...