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Citations for Peprotech :
1751 - 1800 of 1838 citations for Dectin 1 CLEC7A Human HEK293 Fc since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2022Quote: ... undifferentiated WT ESCs were seeded at a density of 250.000 cells per well in 6-well plates and treated with 200ng/mL Wnt antagonist Dickkopf 1 (DKK1) (Peprotech) or 10μM iCRT3 [inhibitor of β-catenin–responsive transcription] (Sigma ...
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bioRxiv - Neuroscience 2022Quote: ... and the media was changed to serum-free DMEM/F12 with 1 % neomycin and 25 ng/ml BDNF (Peprotech). The cells were used from day 12 to day 18 after differentiation.
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bioRxiv - Plant Biology 2019Quote: ... cells were stimulated with either 50 ng·ml−1 recombinant hVEGF-165 for 5 min (Peprotech, Rocky Hill, NJ, USA), 20 ng·ml−1 recombinant hPDGF-B for 5 min (Peprotech ...
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bioRxiv - Immunology 2021Quote: ... cultured at 1–2 Mio/ml in 12-well plates in the presence of 50 ng/ml IL4 (PeproTech) and 100 ng/ml GM-CSF (Immunotools ...
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bioRxiv - Immunology 2020Quote: ... cultured at 1–2 Mio/ml in 12-well plates in the presence of 50 ng/ml IL4 (PeproTech) and 100 ng/ml GM-CSF (Immunotools ...
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bioRxiv - Immunology 2020Quote: ... Primary splenic B cell stimulation was performed with αCD40 (1 μg/ml, eBioscience) plus IL4 (20 ng/ml, PeproTech) or with LPS (25 ng/ml ...
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bioRxiv - Bioengineering 2022Quote: ... 20% R-spondin 1 conditioned medium,50% Wnt3a conditioned medium and 10 μM Y-27632 dihydrochloride (PeproTech Cranbury, USA). Culture medium was replenished every two days.
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bioRxiv - Immunology 2022Quote: ... heterologous Human naive T cells from healthy volunteers were sorted as CD3+CD4+CD45RA+CD45RO- and added to the culture at a ratio of 1:10 (DC:T cell) in the presence of IL-2 recombinant cytokine (50 ng/ml) (Peprotech). Five days post co-culture ...
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bioRxiv - Neuroscience 2023Quote: ... and plated at 50,000 cells/cm2 using iN D4 media (NBM media + 1:50 B27 + BDNF, GDNF, CNTF (10 ng/ml, Peprotech). Doxycycline (2ug/ml ...
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bioRxiv - Neuroscience 2024Quote: ... Culture medium was replaced by a 1:2 mixture of Bottenstein–Sato (BS) medium with PDGF-A (0.5%, Peprotech) and NCM ...
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bioRxiv - Immunology 2024Quote: ... stem cell factor (SCF; 5% supernatant of murine SCF-secreting CHO transfectants) and IL-4 (1 ng/ml, PeproTech) were added to the medium (89) ...
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bioRxiv - Immunology 2023Quote: ... The following anti-mouse primary antibodies were used: anti-RELM-beta (1:500, rabbit polyclonal antibody, Peprotech, 500-p215), anti-MUC2 (1:200 ...
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bioRxiv - Cancer Biology 2024Quote: ... Growth factors were also given to media surrounding basement membrane extract (Cultrex, Biotechne): 1% Mouse recombinant Noggin (Peprotech, UK), 1% mouse recombinant EGF (Invitrogen ...
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bioRxiv - Molecular Biology 2023Quote: ... and resuspended in differentiation medium I-10 + M-CSF (i.e., IMDM, 10 % FBS, 1 mg/ml Pen/Strep containing 25 ng/ml recombinant murine M-CSF (PeproTech)) at a density of 1-2 Mio ...
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bioRxiv - Neuroscience 2023Quote: ... and 100 ng/mL of CX3CL1 (Fractalkine/ chemokine (C-X3-C motif) ligand 1) (Peprotech, Catalog No. 300-31) for up to 12 days.
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bioRxiv - Microbiology 2023Quote: ... and 1 µg/mL soluble anti-CD28 (clone CD28.2, eBioscience) in the presence of 50 IU/mL IL-2 (PeproTech) for three days ...
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bioRxiv - Immunology 2024Quote: ... activated OT-1 T cells were initially expanded in IL-2 (20ng/ml) and IL-12 (20ng/ml; Peprotech) before being switched into IL-2 alone ...
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bioRxiv - Cancer Biology 2021Quote: ... SNKO-1 cells were obtained from DSMZ and cultured in RPMI with 10% FSB and 10 ng/ml GM-CSF (PeproTech). EOL-1 cells were obtained from DSMZ and cultured in RPMI with 10% FBS ...
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bioRxiv - Immunology 2021Quote: ... and cultured at 1-2 × 106/ml in 12-well plates with 1 ml of differentiation medium containing 50 ng/ml interleukin 4 (IL-4, 200–04 A, Peprotech) and 100 ng/ml granulocyte-monocyte colony stimulating factor (GM-CSF ...
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bioRxiv - Neuroscience 2021Quote: ... once daily for 14 days starting at P30 with either vehicle (saline) or rhIGF-1 (2.5 mg/kg; Peprotech, USA) dissolved in saline with 0.01% Bovine Serum Albumin (weight/volume).
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bioRxiv - Molecular Biology 2020Quote: ... supplemented with 1% HSA (Baxter) and cytokines (SCF 100ng/mL, FLT3L 100ng/mL and TPO 100ng/mL; all from PeproTech)55 ...
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bioRxiv - Synthetic Biology 2019Quote: ... Cells were maintained in complete RPMI (10% FBS, 1% Pen/strep) with 30 U/mL rIL-2 (Peprotech, 200-02). To infect ...
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bioRxiv - Neuroscience 2021Quote: ... 1% penicillin/streptomycin) was changed every 3-5 days and M-CSF was supplemented (Peprotech 315-02, 5 ng/mL) for 2 weeks ...
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bioRxiv - Neuroscience 2020Quote: ... The final pellets were dissociated with pre-warmed 10% fetal bovine serum in DMEM supplemented with 20 ng mL-1 mouse M-CSF (PeproTech) and plated in culture dishes ...
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bioRxiv - Bioengineering 2021Quote: ... T cells were cultured at a density of 1 million cells/mL in ImmunoCult-XF T cell Expansion Medium (STEMCELL) supplemented with 200 U/mL IL-2 (Peprotech) and stimulated with ImmunoCult Human CD3/CD28/CD2 T cell Activator (STEMCELL ...
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bioRxiv - Cancer Biology 2021Quote: ... Cells were allowed to attach on coverslip for 1 h and then cells were incubated with murine recombinant CCL2 (Peprotech) for 5 h ...
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bioRxiv - Bioengineering 2021Quote: ... the organoids were transferred from the aforementioned PDMS microwells to a previously published spinning bioreactor19 with a speed of 80 revolutions per minute in E6 medium containing 10 ng mL−1 FGF2 (Peprotech), 20 ng mL−1 BDNF (Peprotech) ...
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bioRxiv - Neuroscience 2020Quote: ... Monocyte chemoattractant protein 1/chemokine C-C motif ligand 2 (MCP1/CCL-2) levels were evaluated using a commercially available ELISA kit (PeproTech) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: Splenic T-cells from Ldlr-/- and T-AbcdkoLdlr-/- mice were isolated and stimulated with Mouse T-Activator CD3/CD28 Dynabeads (ratio beads to cells 1:2) in the presence of 100 U/mL IL-2 (Peprotech) and 5 ng/mL TGFβ1 (Peprotech ...
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bioRxiv - Immunology 2019Quote: Human CD14+ monocytes were purified from leukocyte preparations purchased from the New York Blood Center and differentiated into blood-derived macrophages for 1–2 d in 10 ng/ml M-CSF (PeproTech) and RPMI 1640 medium (Life Technologies)/10% defined FBS (Hyclone) ...
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bioRxiv - Immunology 2020Quote: ... and were then cultured at 1-2 x 106/ml in 12-well plates with differentiation media containing 50 ng/ml Interleukin 4 (Peprotech) and 100 ng/ml granulocyte-monocyte colony stimulating factor (GM-CSF ...
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bioRxiv - Cancer Biology 2019Quote: ... and maintained in culture for a total of approximately 2-3 months in DMEM supplemented with 1 ng/mL IL-3 (Peprotech). Mycoplasma testing was not performed ...
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bioRxiv - Immunology 2021Quote: ... Activated C7 cells were concentrated and 4 ml of culture was resuspended in 1 ml RPMI+ containing 10 μg/mL ESAT61-20 peptide + 20 ng/mL IL-12 (Peprotech) + 20 ng/mL IL-2 (Peprotech) ...
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The skin environment controls local dendritic cell differentiation and function through innate IL-13bioRxiv - Immunology 2021Quote: ... were harvested and one million cells were resuspended in 1 mL of TCM in 24 wells plates and stimulated with recombinant mouse IL-13 (Peprotech) at a concentration of 100 ng/mL for 30 min at 37°C ...
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bioRxiv - Immunology 2020Quote: ... the obtained bone marrow cells were cultured in RPMI-1640 containing 10% FBS and 10 ng mL−1 mouse macrophage colony-stimulating factor (M-CSF, Peprotech) in a humidified 5% CO2 atmosphere at 37 °C for six days ...
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bioRxiv - Cell Biology 2021Quote: ... The fused cells were dispersed in 80 ml of GIT medium (Wako, Japan) supplemented with 1 ng/ml IL-6 (PeproTech) and 1×HAT (Kohjin-Bio) ...
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bioRxiv - Immunology 2024Quote: ... Cells were expanded for at least 1 week in the presence of IL-3 and 30 ng/mL recombinant SCF (Peprotech) to obtain peritoneal cavity-derived mast cells (PCMCs) ...
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bioRxiv - Developmental Biology 2024Quote: ... cells were replated as single cells onto poly-D-lysine-laminin-coated plates at 1×106 cells/cm2 for astrogenic switch and progenitor expansion in N2B27 media supplemented with 10 ng/mL FGF2 (Peprotech) and 10 ng/mL Human EGF (Peprotech ...
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bioRxiv - Immunology 2024Quote: ... PBL, #12400-1; 200U IL-1α #211-11A, 200U IL-1β #211-11Β or both together at 200U total, PeproTech) to allow for pulmonary delivery one week (unless otherwise stated in the figure legends ...
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bioRxiv - Genomics 2023Quote: ... and 3 x 106 cells were plated to grow in suspension in 90 mm bacterial petri dishes containing N2B27 media supplemented with 1 μg/ml doxycycline and 10 ng/ml EGF and FGF (Peprotech). Embryoid-body-like aggregates were collected by mild centrifugation (100 g for 2 min ...
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bioRxiv - Biochemistry 2022Quote: ... For dot blot analysis of BMP-7 in conditioned media from co-culture assays 5 µl were applied to a nitrocellulose membrane and BMP-7 was detected using a rabbit pAb anti-BMP-7 GF at 1:2000 (#500-P198 B, Peprotech). For dot blot analysis in conditioned media of 3D organotypic co-cultures ...
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bioRxiv - Immunology 2023Quote: ... HeLa-sia) at a ratio of 1:100 in complete RPMI medium supplemented with 10 ng/mL GM-CSF (PeproTech). Cancer cells were seeded at an initial concentration of 1×10e4 cells/mL and the same amount of medium supplemented with GM-CSF was added at day 4 of the experiment ...
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bioRxiv - Neuroscience 2023Quote: ... with the addition of growth factors (10 ng/mL BDNF, Peprotech #450-02; 10 ng/mL GDNF, Peprotech #450-10; 1 ng/mL TGF-β1, Peprotech #100-21C ...
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bioRxiv - Immunology 2024Quote: ... Cultures were tested with the indicated concentrations of anti-CD154 (MR-1) or TGF-β+IL-2 (both from Peprotech). In all cases ...
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bioRxiv - Immunology 2024Quote: ... The isolated pDCs (85% purity) were maintained overnight in the presence of IL-3 (1 ng/ml) (PeproTech, London, UK) before stimulation.
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bioRxiv - Neuroscience 2024Quote: ... 0.1% Tween-20 (TBS-T) and bound SDF-1α-CXCL12 chemokine was detected with anti-murine SDF-1α-CXCL12 antibody (diluted 1:2000, Peprotech) followed by an HRP-conjugated anti-rabbit antibody (diluted 1:16000 ...
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bioRxiv - Immunology 2024Quote: ... PBMC were thawed and recovered in complete RPMI overnight without exogenous cytokines before being stimulated with either 1 ng/mL of IL-15 (Peprotech) or 1 ng/mL of IL-15+ 5 ng/mL TGF-β (Peprotech ...
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bioRxiv - Immunology 2024Quote: ... 3×105 CD14+ monocytes were seeded in a 12-well plate in 1 mL complete medium containing 20 ng/mL GM-CSF (#300-03; Peprotech) or 50 ng/mL M-CSF (#300-25 ...
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bioRxiv - Immunology 2021Quote: Both Lin-CD25+CD44- DN3 cells and Lin-CD25-CD44- DN4 cells were sorted from the thymi of LckCre+Zfp335+/+ and LckCre+Zfp335fl/fl mice and co-cultured with OP9-DL1 feeder cells in α-MEM medium in the presence of IL-7 (1 ng/ml, PeproTech) and Flt3-L (5 ng/ml ...
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bioRxiv - Neuroscience 2020Quote: Inflammation in primary glial cultures was induced by the addition of proinflammatory agents such as interleukin 1 beta (IL1β, 10 ng/mL; PeproTech, France) or lipopolysaccharide (LPS ...