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Citations for Peprotech :
1651 - 1700 of 1740 citations for 3 1 3 Dioxan 2 yl 3' methoxypropiophenone since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2021Quote: ... coated plates in serum reduced media (1% HS) supplemented with 50 ng/mL of murine β-NGF (Peprotech, Israel).
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Variation in Leishmania chemokine suppression driven by diversification of the GP63 virulence factorbioRxiv - Microbiology 2021Quote: ... normalized live parasites (1×106) or heterologously expressed GP63 was incubated with 500pg/ml of human recombinant CXCL10 (Peprotech) for the indicated time at 37°C ...
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bioRxiv - Developmental Biology 2022Quote: ... undifferentiated WT ESCs were seeded at a density of 250.000 cells per well in 6-well plates and treated with 200ng/mL Wnt antagonist Dickkopf 1 (DKK1) (Peprotech) or 10μM iCRT3 [inhibitor of β-catenin–responsive transcription] (Sigma ...
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bioRxiv - Neuroscience 2022Quote: ... and the media was changed to serum-free DMEM/F12 with 1 % neomycin and 25 ng/ml BDNF (Peprotech). The cells were used from day 12 to day 18 after differentiation.
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bioRxiv - Immunology 2020Quote: ... Primary splenic B cell stimulation was performed with αCD40 (1 μg/ml, eBioscience) plus IL4 (20 ng/ml, PeproTech) or with LPS (25 ng/ml ...
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bioRxiv - Immunology 2023Quote: ... The following anti-mouse primary antibodies were used: anti-RELM-beta (1:500, rabbit polyclonal antibody, Peprotech, 500-p215), anti-MUC2 (1:200 ...
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bioRxiv - Immunology 2024Quote: ... stem cell factor (SCF; 5% supernatant of murine SCF-secreting CHO transfectants) and IL-4 (1 ng/ml, PeproTech) were added to the medium (89) ...
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bioRxiv - Cancer Biology 2024Quote: ... Growth factors were also given to media surrounding basement membrane extract (Cultrex, Biotechne): 1% Mouse recombinant Noggin (Peprotech, UK), 1% mouse recombinant EGF (Invitrogen ...
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bioRxiv - Molecular Biology 2023Quote: ... and resuspended in differentiation medium I-10 + M-CSF (i.e., IMDM, 10 % FBS, 1 mg/ml Pen/Strep containing 25 ng/ml recombinant murine M-CSF (PeproTech)) at a density of 1-2 Mio ...
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bioRxiv - Neuroscience 2023Quote: ... and 100 ng/mL of CX3CL1 (Fractalkine/ chemokine (C-X3-C motif) ligand 1) (Peprotech, Catalog No. 300-31) for up to 12 days.
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bioRxiv - Bioengineering 2022Quote: ... 20% R-spondin 1 conditioned medium,50% Wnt3a conditioned medium and 10 μM Y-27632 dihydrochloride (PeproTech Cranbury, USA). Culture medium was replenished every two days.
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bioRxiv - Neuroscience 2023Quote: ... and plated at 50,000 cells/cm2 using iN D4 media (NBM media + 1:50 B27 + BDNF, GDNF, CNTF (10 ng/ml, Peprotech). Doxycycline (2ug/ml ...
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bioRxiv - Immunology 2024Quote: ... and 1% penicillin/streptomycin antibiotics and differentiated in the presence of 20ng/mL GM-CSF (Peprotech cat: 300-03) and 10ng/mL M-CSF (Peprotech cat ...
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bioRxiv - Molecular Biology 2024Quote: ... Primocin was removed and Noggin-conditioned media was replaced with recombinant Noggin (100 ng ml−1, 250-38, Peprotech). A day before the electroporation ...
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bioRxiv - Neuroscience 2024Quote: ... The NB-B27-S included human ciliary neurotrophic factor (CNTF, 10 ng/mL, 1:1000, Peprotech, AF-450-13), glial-derived neurotrophic factor (GDNF ...
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bioRxiv - Neuroscience 2024Quote: ... and plated at 50,000 cells/cm2 using iN D4 media (NBM media + 1:50 B27 + BDNF, GDNF, CNTF (10 ng/ml, Peprotech). Doxycycline (2ug/ml ...
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bioRxiv - Bioengineering 2024Quote: ... and 1% Penicillin-Streptomycin (Themo Fisher, CAT: 15070063) and 25 ng/ml of M-CSF (Peprotech, CAT: 315-02). Media was changed after 3 days ...
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bioRxiv - Cancer Biology 2021Quote: ... SNKO-1 cells were obtained from DSMZ and cultured in RPMI with 10% FSB and 10 ng/ml GM-CSF (PeproTech). EOL-1 cells were obtained from DSMZ and cultured in RPMI with 10% FBS ...
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bioRxiv - Immunology 2021Quote: ... and cultured at 1-2 × 106/ml in 12-well plates with 1 ml of differentiation medium containing 50 ng/ml interleukin 4 (IL-4, 200–04 A, Peprotech) and 100 ng/ml granulocyte-monocyte colony stimulating factor (GM-CSF ...
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bioRxiv - Neuroscience 2021Quote: ... once daily for 14 days starting at P30 with either vehicle (saline) or rhIGF-1 (2.5 mg/kg; Peprotech, USA) dissolved in saline with 0.01% Bovine Serum Albumin (weight/volume).
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bioRxiv - Molecular Biology 2020Quote: ... supplemented with 1% HSA (Baxter) and cytokines (SCF 100ng/mL, FLT3L 100ng/mL and TPO 100ng/mL; all from PeproTech)55 ...
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bioRxiv - Neuroscience 2020Quote: ... The final pellets were dissociated with pre-warmed 10% fetal bovine serum in DMEM supplemented with 20 ng mL-1 mouse M-CSF (PeproTech) and plated in culture dishes ...
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bioRxiv - Cancer Biology 2021Quote: ... Cells were allowed to attach on coverslip for 1 h and then cells were incubated with murine recombinant CCL2 (Peprotech) for 5 h ...
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bioRxiv - Bioengineering 2021Quote: Lyophilized 90 % PEGDMA microrods were loaded by resuspending ∼100,000 (100 k) microrod aliquots in 20 μL of 1 mg/mL recombinant human β-NGF (Peprotech) as described in the Protein loading of PEGDMA microrods section ...
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bioRxiv - Microbiology 2020Quote: ... The cells were cultured in RPMI 1640 (Nissui) supplemented with 10% heat-inactivated calf serum (Biowest) and 1 ng/mL recombinant human interleukin 6 (IL-6, PeproTech). HeLa and 293T cells were cultured in DMEM (Nacalai tesque ...
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bioRxiv - Bioengineering 2021Quote: ... the organoids were transferred from the aforementioned PDMS microwells to a previously published spinning bioreactor19 with a speed of 80 revolutions per minute in E6 medium containing 10 ng mL−1 FGF2 (Peprotech), 20 ng mL−1 BDNF (Peprotech) ...
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bioRxiv - Immunology 2021Quote: The differentiation of isolated monocytes into macrophages was performed in RPMI 1640 GlutaMAx medium with 10% FCS and 1% P/S containing 100 ng/mL macrophage colony-stimulating factor (M-CSF) (PeproTech) for 7 days ...
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bioRxiv - Immunology 2021Quote: ... Activated C7 cells were concentrated and 4 ml of culture was resuspended in 1 ml RPMI+ containing 10 μg/mL ESAT61-20 peptide + 20 ng/mL IL-12 (Peprotech) + 20 ng/mL IL-2 (Peprotech) ...
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The skin environment controls local dendritic cell differentiation and function through innate IL-13bioRxiv - Immunology 2021Quote: ... were harvested and one million cells were resuspended in 1 mL of TCM in 24 wells plates and stimulated with recombinant mouse IL-13 (Peprotech) at a concentration of 100 ng/mL for 30 min at 37°C ...
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bioRxiv - Immunology 2020Quote: ... the obtained bone marrow cells were cultured in RPMI-1640 containing 10% FBS and 10 ng mL−1 mouse macrophage colony-stimulating factor (M-CSF, Peprotech) in a humidified 5% CO2 atmosphere at 37 °C for six days ...
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bioRxiv - Cell Biology 2021Quote: ... The fused cells were dispersed in 80 ml of GIT medium (Wako, Japan) supplemented with 1 ng/ml IL-6 (PeproTech) and 1×HAT (Kohjin-Bio) ...
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bioRxiv - Cell Biology 2023Quote: ... cells were either given fresh MC medium or cultured in basal MC medium (1% FBS) or in basal MC medium supplemented with 50ng/mL recombinant human VEGF-A165 (100-20; Peprotech) and culture for another 3 days.
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bioRxiv - Developmental Biology 2024Quote: ... cells were replated as single cells onto poly-D-lysine-laminin-coated plates at 1×106 cells/cm2 for astrogenic switch and progenitor expansion in N2B27 media supplemented with 10 ng/mL FGF2 (Peprotech) and 10 ng/mL Human EGF (Peprotech ...
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bioRxiv - Immunology 2024Quote: ... PBL, #12400-1; 200U IL-1α #211-11A, 200U IL-1β #211-11Β or both together at 200U total, PeproTech) to allow for pulmonary delivery one week (unless otherwise stated in the figure legends ...
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bioRxiv - Genomics 2023Quote: ... and 3 x 106 cells were plated to grow in suspension in 90 mm bacterial petri dishes containing N2B27 media supplemented with 1 μg/ml doxycycline and 10 ng/ml EGF and FGF (Peprotech). Embryoid-body-like aggregates were collected by mild centrifugation (100 g for 2 min ...
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bioRxiv - Neuroscience 2023Quote: ... with the addition of growth factors (10 ng/mL BDNF, Peprotech #450-02; 10 ng/mL GDNF, Peprotech #450-10; 1 ng/mL TGF-β1, Peprotech #100-21C ...
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bioRxiv - Microbiology 2023Quote: ... Monocyte-derived DCs were differentiated by stimulating THP-1 cells with human GMCSF (1500 IU/ml) and IL-4 (1500 IU/ml) (Peprotech) for 5 days with half the medium replaced at day 3 ...
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bioRxiv - Biochemistry 2022Quote: ... For dot blot analysis of BMP-7 in conditioned media from co-culture assays 5 µl were applied to a nitrocellulose membrane and BMP-7 was detected using a rabbit pAb anti-BMP-7 GF at 1:2000 (#500-P198 B, Peprotech). For dot blot analysis in conditioned media of 3D organotypic co-cultures ...
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bioRxiv - Immunology 2023Quote: ... HeLa-sia) at a ratio of 1:100 in complete RPMI medium supplemented with 10 ng/mL GM-CSF (PeproTech). Cancer cells were seeded at an initial concentration of 1×10e4 cells/mL and the same amount of medium supplemented with GM-CSF was added at day 4 of the experiment ...
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bioRxiv - Immunology 2024Quote: ... 3×105 CD14+ monocytes were seeded in a 12-well plate in 1 mL complete medium containing 20 ng/mL GM-CSF (#300-03; Peprotech) or 50 ng/mL M-CSF (#300-25 ...
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bioRxiv - Immunology 2024Quote: ... Sorted BM Ly6Chi monocytes were cultured for four days in coated plates in the presence of CSF-1 (10ng/ml, Peprotech) at 37°C ...
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bioRxiv - Neuroscience 2024Quote: ... 0.1% Tween-20 (TBS-T) and bound SDF-1α-CXCL12 chemokine was detected with anti-murine SDF-1α-CXCL12 antibody (diluted 1:2000, Peprotech) followed by an HRP-conjugated anti-rabbit antibody (diluted 1:16000 ...
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bioRxiv - Immunology 2024Quote: ... PBMC were thawed and recovered in complete RPMI overnight without exogenous cytokines before being stimulated with either 1 ng/mL of IL-15 (Peprotech) or 1 ng/mL of IL-15+ 5 ng/mL TGF-β (Peprotech ...
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bioRxiv - Genomics 2024Quote: ... and seeded to 0.1% gelatinized tissue culture plates in N2B27 supplemented with 10ng/ml FGF2 and 10ng/ml epithelial growth factor (EGF) (PeproTech). NPCs were cultured in 0.1% gelatin-coated plates in N2B27 supplemented with 10ng/ml FGF2 and 10ng/ml EGF.
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bioRxiv - Developmental Biology 2024Quote: ... The plate was placed in 37°C incubator for 60 minutes to solidify the Matrigel and then overlaid with basic differentiation medium containing 50 ng ml−1 of GDNF (PeproTech), 50 ng ml−1 of FGF10 (PeproTech) ...
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bioRxiv - Immunology 2021Quote: Both Lin-CD25+CD44- DN3 cells and Lin-CD25-CD44- DN4 cells were sorted from the thymi of LckCre+Zfp335+/+ and LckCre+Zfp335fl/fl mice and co-cultured with OP9-DL1 feeder cells in α-MEM medium in the presence of IL-7 (1 ng/ml, PeproTech) and Flt3-L (5 ng/ml ...
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bioRxiv - Neuroscience 2020Quote: Inflammation in primary glial cultures was induced by the addition of proinflammatory agents such as interleukin 1 beta (IL1β, 10 ng/mL; PeproTech, France) or lipopolysaccharide (LPS ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: HBD1 and LL-37 peptide release was quantified in supernatants from 25 day long-term cultured A549 cells using a human BD-1 Standard ABTS ELISA Development Kit (PeproTech, Germany) and LL-37 human ELISA kit (HycultBiotech ...
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bioRxiv - Immunology 2020Quote: ... Rinse solution was then aspirated and replaced with 1 mL of 2X concentrated EB medium (1X EB media: OXE8 medium supplemented with 50 ng/mL BMP4 (Peprotech, #PHC9534), 50 ng/mL VEGF (Peprotech ...
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bioRxiv - Neuroscience 2022Quote: ... in DMEM medium (+10 % FCS and 1% P/S) and stimulated with either 10 ng/ml TNF-α and 10 ng/ml IL-1β (both from Peprotech) or vehicle control ...