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Citations for Peprotech :
1501 - 1550 of 1586 citations for Dengue Virus Serotype 3 Envelope Protein Human Fc Tag HEK293 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2022Quote: ... 10 ng/mL IL-3 (Cat# 200-03) and 10 ng/mL G-CSF (Cat# 300-23; all cytokines from Peprotech), 100 IU/mL penicillin ...
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bioRxiv - Bioengineering 2022Quote: ... 0.1% BSA Fraction V [Gemini Bio]) supplemented with CK+DCIR additives (3 μM CHIR99021 [CHIR; PeproTech], 10 ng/mL keratinocyte growth factor [KGF; PeproTech] ...
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bioRxiv - Molecular Biology 2022Quote: ... supplemented with cytokines (150 ng/ml SCF, PeproTech, Cat 300-07; 150 ng/ml Flt-3, PeproTech, Cat 300-19; 10 ng/ml IL-6, PeproTech, Cat 200-06 ...
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bioRxiv - Molecular Biology 2022Quote: ... 150 ng/ml Flt-3, PeproTech, Cat 300-19; 10 ng/ml IL-6, PeproTech, Cat 200-06; 25 ng/ml G-CSF, PeproTech Cat 300-23 ...
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bioRxiv - Cancer Biology 2019Quote: ... The 32D-BCR-ABL and the IL-3-dependent parental 32Dcl3 mouse myeloid precursors were cultured in Iscove modified Dulbecco Medium (IMDM) supplemented with 10% FBS and rmIL-3 (2ng/ml, Peprotech). Primary cells ...
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bioRxiv - Cancer Biology 2020Quote: ... 100 ng/mL fms-like tyrosine kinase 3 ligand (Flt-3L) and 50 ng/mL thrombopoietin (TPO) (all from Peprotech). ES and IPS derived EB were cultured in ultra low attachment 6-well plates (Costar ...
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bioRxiv - Molecular Biology 2021Quote: ... cells were dissociated and 3×106 cells were plated in a bacterial petri dish in N2B27 medium supplemented with EGF 10 ng/mL (Peprotech) and FGF2 10 ng/mL (R&D systems) ...
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bioRxiv - Cancer Biology 2022Quote: ... 100 ng/mL fms-like tyrosine kinase 3 ligand (Flt-3L) and 50 ng/mL thrombopoietin (TPO) (all from Peprotech). ES and IPS derived EB were cultured in ultra-low attachment 6-well plates (Costar ...
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bioRxiv - Cell Biology 2022Quote: ... The monolayer was washed 3 times with HBSS to remove non-adherent cells and monocytes were differentiated into macrophages for 3 days in RPMI media + 10% FBS containing 30 ng/ml M-CSF (PeproTech) and for further 4 days in media containing 30 ng/ml RANKL (PeproTech ...
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bioRxiv - Developmental Biology 2022Quote: ... 150 μl of N2B27 medium supplemented with 100 ng/ml Activin A (QKine, Cambridge, UK) and 3 μM chiron (Peprotech) was added to each well ...
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bioRxiv - Bioengineering 2023Quote: ... One half was plated in SCGM medium supplemented with cytokines and 10ng/ml IL-3 (PeproTech, Rocky Hill, NJ, USA) as +IL-3 treatment ...
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bioRxiv - Neuroscience 2023Quote: ... and plated onto 3 wells of a 6-well plate precoated with GFR Matrigel in N2B27 with 100ng/ml FGFb (100-18B, Peprotech) and 100ng/ml EGF (AF-100-15 ...
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bioRxiv - Cancer Biology 2023Quote: ... HSCL or fetal liver LSK cells were cultured for 2 weeks on OP9 or OP9-DL4 cell layer in HSCL medium supplemented with 10 ng/mL Flt-3 (PeproTech) and 10 ng/mL IL-7 (PeproTech).
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bioRxiv - Immunology 2023Quote: ... Cells were cultured with stem cell factor (SCF) and FMS-like tyrosine kinase 3 ligand (FLT3L) (Peprotech, Rocky Hill, USA) for the first 4 days ...
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bioRxiv - Immunology 2023Quote: ... bone marrow was taken from the tibias and femurs of 9-week-old C57BL/6 Lgals9 -/- mice or wild type litter mates and cultured in RPMI containing 10 % FBS and 3 % granulocyte macrophage colony-stimulating factor (mGM-CSF) (Peprotech). for 7 days ...
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bioRxiv - Cell Biology 2023Quote: ... glutamine and human early-acting cytokines (SCF 300 ng/ml, Flt3-L 300 ng/ml, TPO 100 ng/ml, and IL-3 60 ng/ml; all purchased from Peprotech). All cultures were kept at 37°C in a 5% CO2 water jacket incubator (Thermo Scientific ...
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bioRxiv - Immunology 2024Quote: ... Cells were expanded for at least 1 week in the presence of IL-3 and 30 ng/mL recombinant SCF (Peprotech) to obtain peritoneal cavity-derived mast cells (PCMCs) ...
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bioRxiv - Cancer Biology 2024Quote: ... containing recombinant mouse cytokines (20 ng/ml IL-3, 50 ng/ml IL-6, 10 ng/ml Flt3L and 50 ng/ml SCF, PeproTech), 2 mM L-glutamine ...
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Nanoparticle-delivered TLR4 and RIG-I agonists enhance immune response to SARS-CoV-2 subunit vaccinebioRxiv - Immunology 2022Quote: ... RBC-lysed murine bone marrow was seeded at 2 million cells/mL in complete RPMI media with 200 ng/mL human FLT3 ligand (PeproTech, Cat# 300-19) in a 6-well plate (10 million cells/well) ...
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bioRxiv - Immunology 2022Quote: ... The CD34 cell purity was checked by FACS and cells were cultured in IMDM with 10% HI FBS supplemented with human cytokine and growth factor cocktail from Peprotech (hTPO (#300-18) (10 ng/ml) ...
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bioRxiv - Cancer Biology 2021Quote: ... The cells were incubated in RPMI1640 for THP-1 culture supplemented with human M-CSF at 20 ng/ml (cross-react with mouse, Peprotech, Cranbury, NJ, USA). After 2 days of culture ...
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bioRxiv - Microbiology 2021Quote: ... control and CSP-stable transfected HepG2 cells were pre-treated with 1 U/mL recombinant human IFN-γ (Peprotech, Rocky Hill, NJ, USA) or equivoluminal medium for 6 h ...
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bioRxiv - Immunology 2020Quote: ... 1640 Medium supplemented with 10% fetal bovine serum (35-076-CV; Coring, New York, USA) and 20 ng/μL Recombinant Human GM-CSF (300-03; Peprotech, Rocky Hill, USA).BEAS-2B cells (CRL-9609 ...
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bioRxiv - Cancer Biology 2021Quote: ... MCF7 cells were cultured in 2D in the absence or presence of 10 ng/mL human TGFβ (PeproTech®, Rocky Hill, NJ, USA) for 3 weeks where TGFβ was replenished every 2 days ...
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bioRxiv - Immunology 2021Quote: ... Human monocyte-derived macrophages (HMDMs) were differentiated from purified CD14+ monocytes in the presence of recombinant human M-CSF (50 ng/ml; PeproTech, Cranbury, NJ, USA) for 6 days in RPMI 1640 medium supplemented with 10% FBS ...
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bioRxiv - Immunology 2020Quote: ... Isolated PBMC were washed with PBS and resuspended by RPMI-1640 which supplemented 50ng/ml recombinant human macrophage colony-stimulating factor (M-CSF) (AF-300-25-500, PeproTech, Cranbury, NJ, USA), then cultured at 37°C in 5% CO2 for 5 days to differentiate into human monocyte derived macrophages (hMDMs ...
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bioRxiv - Immunology 2022Quote: ... Cells were differentiated to macrophages in RPMI x1640 supplemented with 10% FBS containing 10 ng/ml of recombinant human GM-CSF (Peprotech, New Jersey, US) for 7 days ...
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bioRxiv - Bioengineering 2022Quote: ... MDTs were exposed to a neutral culture medium or to a 20 ng/ml of TNF solution (Recombinant TNF alpha human; 300-01A, PeproTech, Thermo Fisher Scientific) in culture medium for either 30 minutes or 240 minutes ...
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bioRxiv - Immunology 2023Quote: ... were differentiated from PBMCs by culturing the cells in RPMI complete medium supplemented with 10% AB-positive human serum and 15 ng/ml M-CSF (PeproTech, 300-25-100UG). After 2 days of culturing ...
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bioRxiv - Developmental Biology 2024Quote: ... proteins were supplemented in Maturation media by dissolving Matrigel at 1% (v/v) containing human recombinant brain-derived neurotrophic factor (BDNF; PeproTech, AF-450-02). The aggregated cells were referred to as cerebral organoids from this stage onward and were allowed to further develop in maturation media until the experimental endpoints ...
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bioRxiv - Immunology 2023Quote: PBMC from healthy donor buffy coats were isolated by Ficoll density centrifugation and rested overnight in 10 ng/mL recombinant human IL-15 (Peprotech, cat #200-15) at 2*106 cells/mL cell culture media (RPMI-1640 ...
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bioRxiv - Immunology 2023Quote: Transplant recipient and healthy donor cryopreserved PBMC were thawed and recovered overnight in 10 ng/mL recombinant human IL-15 (Peprotech, cat #200-15) at 2*106 cells/mL cell culture media (RPMI-1640 ...
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bioRxiv - Immunology 2023Quote: ... iPSCs were differentiated to macrophages[73] and stimulated for 6h and 24h with 10 ng/mL recombinant human interleukin-10 (Peprotech, 200-10-2), 10 ng/mL recombinant human interferon-b (Peprotech ...
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bioRxiv - Genomics 2024Quote: ... For M2 polarization macrophages were cultured in macrophage media supplemented with 20 ng/ml recombinant human IL-4 (PeproTech, catalog no. 200-04). Cells were harvested after 24 ...
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bioRxiv - Cancer Biology 2022Quote: ... Cells were afterwards stimulated with indicated amount of recombinant protein CXCL2 (250-15-20, PeproTech GmbH) in DMEM medium without FCS or conditioned medium of endothelial cells.
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bioRxiv - Microbiology 2021Quote: ... cells were starved for 3 h by removal of serum from the medium and then stimulated in duplicate with TNF-α (40 ng/ml, PeproTech) in FBS-free DMEM for 0 ...
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bioRxiv - Cancer Biology 2022Quote: ... were plated onto 24-well plates in Matrigel and cultured for 3 days before treatment with either recombinant murine INF-y (PeproTech, UK) at 1 ng/mL or recombinant murine TGFβ 1 (R&D ...
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bioRxiv - Cell Biology 2023Quote: ... On day 3, medium was replaced with E6 supplemented with 50 ng/mL vascular endothelial growth factor (VEGF, (PeproTech, 100-20), 10 ng/mL FGF2 and 20 ng/mL rBMP4 ...
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bioRxiv - Immunology 2024Quote: ... together with 10 ng/mL recombinant mouse IL-4 or 3 μg/mL LPS with 10 ng/mL recombinant murine IFN-ψ (Peprotech) and 20 ng/mL recombinant human BAFF or 5 μg/mL anti-CD40 (Bio X Cell ...
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bioRxiv - Bioengineering 2021Quote: PTMs were differentiated from hiPSCs using the above protocol for hiPSC-derived Leydig cells with the modification that PDGF-AA is replaced by human recombinant Platelet-Derived Growth Factor-BB (PDGF-BB, Peprotech, 100-14B, Figure 1B). On day 25 the cells were expanded on PLL-coated plates in DMEM/F12 ...
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bioRxiv - Neuroscience 2020Quote: ... and 1% insulin transferrin selenium (Cat N° 41400045) in IMDM (Cat N° 12440053) supplemented with 5 ng/ml recombinant human basic fibroblast growth factor (bFGF) (Peprotech, Cat N° 100-18B). Medium was changed every 4 days ...
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bioRxiv - Immunology 2022Quote: ... hCD34+ cells were either kept in culture at 37°C in RPMI medium with human recombinant Stem Cell Factor (SCF) at 50 ng/ml (PeproTech, Rocky Hill, NJ, USA), human recombinant thrombopoietin (TPO ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Differentiated sensory neurons were maintained in N2 medium with neuronal growth factors (10 ng/ml human β-NGF, BDNF, NT3 and GDNF; PeproTech, Rocky Hill, NJ) in a 37°C incubator under 5% CO2 ...
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bioRxiv - Microbiology 2021Quote: ... 2.5×105 HUVECs were plated in wells of 6-well plates and stimulated overnight with 20 ng.ml−1 human recombinant TNFα (PeproTech/Tebu Bio, #300-01A-A) or infected at MOI=200 with Neisseria meningitidis for 30 min ...
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bioRxiv - Neuroscience 2022Quote: ... and plated on low-attachment 6-well plate in E8 with 20 ng/ml human basic fibroblast growth factor (bFGF2; Peprotech, Somerset County, NJ, USA) and 20 µM Rho kinase inhibitor (Sigma-Aldrich ...
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bioRxiv - Biophysics 2023Quote: ... we tuned the cellular contractility by replacing the medium with a new one containing recombinant human TGF-β (2.5 ng/mL, 100-21, PeproTech, Rocky Hill, NJ, USA), to promote cellular contractility ...
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bioRxiv - Cancer Biology 2023Quote: ... Measurement of IL-6 levels was performed by sandwich ELISA using the human IL-6 mini ABTS ELISA development kit (Peprotech-catalog no 900-M16).
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bioRxiv - Cell Biology 2023Quote: ... cells were plated in 1% FBS- containing DMEM for 72 hrs with or without 4ng/mL human recombinant TGF-β (Peprotech; cat #100-21).
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bioRxiv - Cancer Biology 2023Quote: ... the FGF and EGF mitogens in the media were replaced by 100 ng/ml of BMP4 (Recombinant human BMP-4, PeproTech, Cat# AF-120-05ET) (quiescence media) ...
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bioRxiv - Cell Biology 2020Quote: ... the organoids were grown in DM supplemented with bone morphogenetic protein 4 (BMP4) (50 ng/mL, PeproTech) or Purmorphamine (1 μm ...