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Citations for Peprotech :
1451 - 1500 of 1536 citations for Recombinant Swine CCL19 Protein since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... 55 uM 2-mercaptoethanol and 20 ng/ml of recombinant murine granulocyte-macrophage colony-stimulating factor (PeproTech, Rocky Hill, NJ, United States)] at 1:5 apoptotic cancer cell to cDC1 ratio ...
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bioRxiv - Immunology 2023Quote: All cell lines were seeded in flat 96-well plates at a density of 0.25 × 105 cells/well and cultured for 24 h in the absence or presence of human recombinant IFNγ and TNFα with activities of 2 × 107 Units/mg (PeproTech, Rehovot, Israel). Cells lines were then co-cultured with 100,000 (at density of 1×106 cell/ml ...
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bioRxiv - Immunology 2023Quote: ... Bone marrow was plated into non-cell-culture treated 10cm petri dishes in 8mL supplemented RPMI medium containing either 20ng/mL recombinant M-CSF (Peprotech, #315-02) or 20ng/mL recombinant GM-CSF (Peprotech ...
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bioRxiv - Bioengineering 2023Quote: ... Primary human T cells were cultured in complete RPMI 1640 supplemented with 100□U/ml recombinant human IL-2 (PeproTech, 200-02). All mammalian cells were cultured at 37□°C in a humidified 5% CO2 incubator.
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bioRxiv - Microbiology 2023Quote: ... Plates were incubated for 15 min at 37°C to allow the Matrigel to polymerize and then overlaid with 300 µl of growth medium (basal medium supplemented with 12.5 ng/ml mouse recombinant epithelial growth factor (PeproTech, Rocky Hill, NJ), 25 ng/ml mouse recombinant Noggin (PeproTech) ...
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bioRxiv - Immunology 2023Quote: ... 10 million cells were resuspended in 10 ml medium and stimulated with 25ng/ml recombinant human interleukin-2 (IL-2) (Peprotech #AF200-02). Vγ9+Vδ2+ T cells were kept overnight at 37°C and 5% CO2.
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bioRxiv - Biochemistry 2024Quote: ... Sigma-Aldrich P6407) in OPC proliferation media (DMEM-Sato) supplemented with 10 ng/ml recombinant human ciliary neurotropic factor (CNTF; Peprotech 450-13), 10 µM forskolin (Sigma-Aldrich F6886) ...
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bioRxiv - Immunology 2024Quote: ... pH 7.34 for a short stimulation of 30 min) or in a CBMC medium supplemented with recombinant h-SCF (100 ng/mL, Gibco, PeproTech, #300-07) (for a longer stimulation) ...
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bioRxiv - Neuroscience 2024Quote: MRMT-1 cells were treated with DsiRNA-containing lipid nanoparticles (5 µg/ml) containing DID dye in the presence or absence of recombinant 10 µg/ml ApoE4 (PeproTech #350-04) for 24 h ...
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bioRxiv - Neuroscience 2024Quote: ... one well of dissRO and iMG-dissRO were treated with recombinant human CCL2 (C-C Motif Chemokine Ligand 2, Peprotech, #300-04) at a final concentration of 10 ng/mL ...
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IL-6 SIGNALING EXACERBATES HALLMARKS OF CHRONIC TENDON DISEASE BY STIMULATING REPARATIVE FIBROBLASTSbioRxiv - Bioengineering 2024Quote: ... with two media changes per week until the determined timepoint.[71] We used a final concentration of 25 ng/ml recombinant IL-6 (PeproTech, 216-16) in those assembloids to be stimulated by it ...
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bioRxiv - Cancer Biology 2024Quote: ... Exploration of BMP2 effect on HSJD-DIPG-012 and HSJD-DIPG-014 spheroids was done using 10 ng/mL of human recombinant BMP2 (Peprotech, 120-02C) before protein quantification by western blot.
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bioRxiv - Immunology 2024Quote: ... bone marrow was flushed from femurs and tibias of mice and cells were plated and cultured in the presence of recombinant macrophage colony-stimulating factor (M-CSF; 20 ng/ml, PeproTech or Proteintech). Culture medium was replaced every two days ...
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bioRxiv - Bioengineering 2024Quote: ... in T75 flasks at 2 million/mL and differentiated for 7-9 days with 50 ng/mL recombinant human M-CSF (300-25, PeproTech, Cranbury, NJ) and 20 ng/mL recombinant human IL-4 (200-04 ...
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bioRxiv - Biochemistry 2024Quote: ... the media was then exchanged for 2ml/well of mesoderm induction media (MIM), composed of STEMdiff APEL 2 Medium (STEMCELL, 05275) + 50ng/ml Recombinant Human FGF-basic (Peprotech, 100-18B), 50ng/ml Human BMP-4 (E.coli derived ...
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bioRxiv - Physiology 2022Quote: ... to trigger their differentiation towards a pro-inflammatory phenotype M(LPS,IFN) or IL4 (20 ng ml−1, rat recombinant; Peprotech, London, United Kingdom) to obtain anti-inflammatory macrophages ...
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bioRxiv - Cancer Biology 2022Quote: ... Cells were then cultured on untreated plates for 7 days adding 10 ng/ml recombinant murine M-CSF (Peprotech, AF-315-02-100) at day of isolation ...
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bioRxiv - Microbiology 2021Quote: ... control and CSP-stable transfected HepG2 cells were pre-treated with 1 U/mL recombinant human IFN-γ (Peprotech, Rocky Hill, NJ, USA) or equivoluminal medium for 6 h ...
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bioRxiv - Immunology 2020Quote: ... 1640 Medium supplemented with 10% fetal bovine serum (35-076-CV; Coring, New York, USA) and 20 ng/μL Recombinant Human GM-CSF (300-03; Peprotech, Rocky Hill, USA).BEAS-2B cells (CRL-9609 ...
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bioRxiv - Developmental Biology 2020Quote: ... LepRb-Cre::tdTom and GCG-Cre::SynTom mice received intraperitoneal (i.p.) injections of recombinant mouse leptin (10mg/kg body weight; Peprotech Inc., Rocky Hill, NJ), or vehicle (0.9% sterile saline) ...
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bioRxiv - Molecular Biology 2021Quote: ... BMDM and AM were cultured in Dulbeccòs Modified Eagle’s Medium (DMEM) + 10% heat-inactivated fetal bovine serum (FBS) supplemented with 20 ng/ml recombinant murine M-CSF (PeproTech, Rocky Hill, NJ) and 20 ng/ml recombinant murine RANKL (PeproTech) ...
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bioRxiv - Immunology 2020Quote: ... Cells were treated with Mouse active perforin (#APB317Mu01, Cloud-Clone Corp., Katy, TX) and recombinant mouse Granzyme B (#140-03, PeproTech, Rocky Hill, NJ) for 4 to 6 hours in indi cated concentrations following manufacturer’s recommendations.
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bioRxiv - Immunology 2021Quote: ... Human monocyte-derived macrophages (HMDMs) were differentiated from purified CD14+ monocytes in the presence of recombinant human M-CSF (50 ng/ml; PeproTech, Cranbury, NJ, USA) for 6 days in RPMI 1640 medium supplemented with 10% FBS ...
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bioRxiv - Immunology 2020Quote: ... Isolated PBMC were washed with PBS and resuspended by RPMI-1640 which supplemented 50ng/ml recombinant human macrophage colony-stimulating factor (M-CSF) (AF-300-25-500, PeproTech, Cranbury, NJ, USA), then cultured at 37°C in 5% CO2 for 5 days to differentiate into human monocyte derived macrophages (hMDMs ...
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bioRxiv - Immunology 2021Quote: ... were plated with the irradiated 40LB cells in R10 media with 1 ng/mL recombinant mouse interleukin-4 (rmIL-4; Peprotech, East Windsor, NJ). On day 4 ...
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bioRxiv - Immunology 2022Quote: ... The following cytokines were added at the indicated concentrations for murine γδT cells: recombinant murine IL-12 (10ng/ml) (Peprotech, Rocky Hill, NJ), IL-15 (50ng/ml ...
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bioRxiv - Immunology 2022Quote: ... Cells were differentiated to macrophages in RPMI x1640 supplemented with 10% FBS containing 10 ng/ml of recombinant human GM-CSF (Peprotech, New Jersey, US) for 7 days ...
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bioRxiv - Bioengineering 2022Quote: ... MDTs were exposed to a neutral culture medium or to a 20 ng/ml of TNF solution (Recombinant TNF alpha human; 300-01A, PeproTech, Thermo Fisher Scientific) in culture medium for either 30 minutes or 240 minutes ...
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bioRxiv - Developmental Biology 2024Quote: ... proteins were supplemented in Maturation media by dissolving Matrigel at 1% (v/v) containing human recombinant brain-derived neurotrophic factor (BDNF; PeproTech, AF-450-02). The aggregated cells were referred to as cerebral organoids from this stage onward and were allowed to further develop in maturation media until the experimental endpoints ...
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bioRxiv - Immunology 2022Quote: ... or CD11b+ enriched single cell suspensions of B16F10 tumors were cultured in the presence of 50 ng/mL recombinant M-CSF (Peprotech, Cranbury, NJ, USA) in RPMI (Wellgene ...
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bioRxiv - Immunology 2023Quote: PBMC from healthy donor buffy coats were isolated by Ficoll density centrifugation and rested overnight in 10 ng/mL recombinant human IL-15 (Peprotech, cat #200-15) at 2*106 cells/mL cell culture media (RPMI-1640 ...
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bioRxiv - Immunology 2023Quote: ... iPSCs were differentiated to macrophages[73] and stimulated for 6h and 24h with 10 ng/mL recombinant human interleukin-10 (Peprotech, 200-10-2), 10 ng/mL recombinant human interferon-b (Peprotech ...
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bioRxiv - Immunology 2023Quote: Transplant recipient and healthy donor cryopreserved PBMC were thawed and recovered overnight in 10 ng/mL recombinant human IL-15 (Peprotech, cat #200-15) at 2*106 cells/mL cell culture media (RPMI-1640 ...
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bioRxiv - Immunology 2024Quote: ... cells were cultured in RPMI 1640 supplemented with 10% FBS and 1X Pen/Strep in the presence of either 1 ng/mL Recombinant murine IL-7 (PeproTech, Cat#217-17) or stimulated with anti-CD3/CD28 Dynabeads at a 4:1 bead:cell ratio (Fisher Scientific ...
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bioRxiv - Immunology 2024Quote: ... Cells were counted and 1×106 cells were plated in R10 containing 10 ng/mL recombinant murine M-CSF (Peprotech; Cat# 315-02) for 2 days ...
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bioRxiv - Immunology 2024Quote: ... at a 1:3 T cell:bead ratio cultured in R10 media and 20 IU of recombinant human IL-2 (Peprotech, Cat-no. 200-02). Cells were transduced with CAR lentivirus at a multiplicity of infection (MOI ...
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bioRxiv - Immunology 2024Quote: ... loosely adherent cells were collected and further cultured in media supplemented with recombinant mouse GM-CSF (20 ng/mL; Peprotech, catalog# 315-03). The same fresh GM-CSF-supplemented media was supplied on day 2 and 4 ...
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bioRxiv - Cancer Biology 2024Quote: ... and expanded with C9 feeder cells at a 1:1 ratio in R10 media supplemented with 200 IU/ml human recombinant IL-2 (PeproTech, Cat# 200-02).
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bioRxiv - Cell Biology 2024Quote: ... 5 ng/ml HGF Protein (PeproTech, 315-23), 10 ng/ml NT-3 Protein (PeproTech ...
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bioRxiv - Immunology 2021Quote: ... cells isolated from the femur and tibia were cultured at 1 × 106 cells/ml in 10 ml of complete DMEM (cDMEM) supplemented with 4% recombinant mouse GM-CSF (Peprotech, Rocky Hill, NJ, USA) at 37°C in 7.5% CO2 ...
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bioRxiv - Bioengineering 2021Quote: PTMs were differentiated from hiPSCs using the above protocol for hiPSC-derived Leydig cells with the modification that PDGF-AA is replaced by human recombinant Platelet-Derived Growth Factor-BB (PDGF-BB, Peprotech, 100-14B, Figure 1B). On day 25 the cells were expanded on PLL-coated plates in DMEM/F12 ...
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bioRxiv - Neuroscience 2020Quote: ... and 1% insulin transferrin selenium (Cat N° 41400045) in IMDM (Cat N° 12440053) supplemented with 5 ng/ml recombinant human basic fibroblast growth factor (bFGF) (Peprotech, Cat N° 100-18B). Medium was changed every 4 days ...
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bioRxiv - Immunology 2022Quote: ... Cells were incubated for 3 hours at 37°C in RPMI media supplemented with or without recombinant mouse IL-21 (20 ng/mL, Peprotech cat. 210-21-100). Cells were stimulated with either anti-Igκ and anti-Igλ or anti-CD40 ...
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bioRxiv - Immunology 2022Quote: ... hCD34+ cells were either kept in culture at 37°C in RPMI medium with human recombinant Stem Cell Factor (SCF) at 50 ng/ml (PeproTech, Rocky Hill, NJ, USA), human recombinant thrombopoietin (TPO ...
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bioRxiv - Immunology 2020Quote: ... extracellular Cn were removed by washing 2X with PBS and the macrophages were cultured for a further 24 h in fresh growth media containing either IFNγ to maintain the M1 polarization state or 100 ng/mL recombinant interleukin-4 (IL-4; Sigma-Aldrich, St. Louis, MO, and Peprotech, Rocky Hill, NJ) to promote repolarization to the M2 state ...
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bioRxiv - Microbiology 2021Quote: ... 2.5×105 HUVECs were plated in wells of 6-well plates and stimulated overnight with 20 ng.ml−1 human recombinant TNFα (PeproTech/Tebu Bio, #300-01A-A) or infected at MOI=200 with Neisseria meningitidis for 30 min ...
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bioRxiv - Biophysics 2023Quote: ... we tuned the cellular contractility by replacing the medium with a new one containing recombinant human TGF-β (2.5 ng/mL, 100-21, PeproTech, Rocky Hill, NJ, USA), to promote cellular contractility ...
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bioRxiv - Cell Biology 2023Quote: ... cells were plated in 1% FBS- containing DMEM for 72 hrs with or without 4ng/mL human recombinant TGF-β (Peprotech; cat #100-21).
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bioRxiv - Cancer Biology 2023Quote: ... the FGF and EGF mitogens in the media were replaced by 100 ng/ml of BMP4 (Recombinant human BMP-4, PeproTech, Cat# AF-120-05ET) (quiescence media) ...
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bioRxiv - Immunology 2024Quote: ... was obtained from the University of Pittsburgh Medical Center Presbyterian Pharmacy and recombinant human IL-2 (IL-2) was obtained from Peprotech (Cat. No. 200-02). One group of SIV-infected juvenile animals (n = 5 ...