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Citations for Peprotech :
1451 - 1498 of 1498 citations for Human Histone H3.3C H3 5 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... for 4 days at 37°C in complete RPMI medium supplemented with 10 ng/mL recombinant mouse IL-5 (PeproTech) and 20 ng/mL IL-2 (402-ML R&D) ...
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bioRxiv - Neuroscience 2020Quote: ... Purified OPCs were expanded in poly-d-lysine coated flasks for 4-5 days in SATO serum-free media containing Platelet-Derived Growth Factor-AA (PDGF-AA, 10 ng/ml, PeproTech), neurotrophin-3 (NT-3 ...
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bioRxiv - Immunology 2022Quote: Cells were cultured in complete T cell media supplemented with 5 ng/mL each of recombinant murine IL-7 and IL-15 (PeproTech). One day prior to activation of OT-I cells ...
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bioRxiv - Molecular Biology 2019Quote: ... MLO-Y4 cells were treated with the following reagents in serum-free media: 5 ng/ml TGFβ1 (Peprotech, 100-36E), 0.1 μM PGE2 (Sigma) ...
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bioRxiv - Systems Biology 2019Quote: ... and 106 cells were transferred onto each retronectin/DL1-coated virus-bound well supplemented with 5 ng/mL SCF (Peprotech), 5 ng/mL Flt3-L ...
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bioRxiv - Systems Biology 2019Quote: ... All in vitro T cell generation cultures were supplemented with 5ng/mL Flt3-L and 5 ng/mL IL-7 (Peprotech), and were sorted after 6 or 7 total days of culture before transducing with retroviral vectors or treating with small molecule inhibitors ...
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bioRxiv - Neuroscience 2021Quote: ... The isolated OPCs were then cultured on PDL-coated plates using GM plus 5 ng/ml FGF (450-33, Peprotech), 4 ng/ml PDGF-AA (315-17 ...
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bioRxiv - Cell Biology 2021Quote: Immunofluorescent staining was in general performed on HUVECS cultured on 12 mm glass coverslips coated with 5 μg/ml FN and treated with or without o/n TNFα (10ng/ml) (Peprotech), washed with PBS+/+(1mM CaCl2 and 0.5mM MgCl2) ...
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bioRxiv - Neuroscience 2022Quote: ... astrocyte cultures at 80% confluence in 6-well plates (in E6 medium containing BMP4 and FGF2) were treated with vehicle control or IL-1a (5 ng/ml; Peprotech), TNFα (10 ng/ml ...
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bioRxiv - Immunology 2022Quote: ... for Th1 polarization the medium was supplemented with 5 ng/ml IL-2 and 10 ng/ml IL-12 (both from Peprotech), and 10 μg/ml anti-IL-4 (11B11 ...
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bioRxiv - Cancer Biology 2022Quote: ... mAb in 200ul of T cell media supplemented with 5 ng/ml of IL-7 and 30 U/ml of IL-2 (PeproTech) for 4 and 7 days ...
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bioRxiv - Immunology 2020Quote: ... adherent monocytes were differentiated into macrophages over a 5 days in the presence of 50 ng/mL GM-CSF (Peprotech) in RPMI supplemented with 10% foetal calf serum (FCS ...
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bioRxiv - Immunology 2020Quote: ... 5 ng/ml of IL-2 was added to the culture and memory T cells were supplemented with 5 ng/ml IL-7 and IL-15 (all Peprotech). Cells were activated in a reverse time-course so that their total time in culture was identical and so that all cells would finish their indicated activation period at the same day to enable extracellular flux analysis ...
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bioRxiv - Cancer Biology 2019Quote: Primary murine macrophages were generated by flushing the bone marrow from the femur and tibia of 6-8 week old C57BL/6 mice followed by incubation for 5 days in DMEM containing 10% FBS and 10 ng/mL murine M-CSF (Peprotech). Primary pancreatic stellate cells were isolated from the pancreas of C57BL/6 mice by density gradient centrifugation ...
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bioRxiv - Genomics 2020Quote: ... 1% Glutamax, and 1% FBS, 8 ng/ml FGF2, 5 ng/ml CNTF, and 10 ng/ml BMP4, from Peprotech). On day 7 ...
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bioRxiv - Genetics 2020Quote: ... and plated on Matrigel-coated plate in a density of 5×104 cells cm-2 in ES cell medium containing 10 ng/mL fibroblast growth factor 2 (FGF2, Peprotech). The differentiation was started by replacing ES cell medium with KSR medium and gradually switched to N2 medium from day 4 to day 10 ...
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bioRxiv - Immunology 2020Quote: Adherent PBMC monolayer was washed twice with HBSS and monocytes were differentiated into hMDMs for 5 days in RPMI 1640 containing 100 ng/mL macrophage colony-stimulating factor (M-CSF, PeproTech) and 10% foetal calf serum ...
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bioRxiv - Bioengineering 2022Quote: ... cells were incubated with StemPro-34 medium from days 2 to 5 with a cocktail of growth factors: vascular endothelial growth factor (VEGF; PeproTech), BMP-4 ...
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bioRxiv - Immunology 2023Quote: ... Isolated T cells were otherwise activated using plate bound anti-CD3 (5 μg/ml, inVIVOMAb Cat# BE0002) in complete medium added with 100 U/ml IL-2 (Peprotech) and soluble αCD28 (0.5 μg/ml ...
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bioRxiv - Immunology 2023Quote: ... supplemented with 5% heat-inactivated fetal bovine serum (Biosera FB-1001) and 50ng/ml recombinant M-CSF (Peprotech 300-25). Differentiated macrophages were plated at a density of 1 x 106ml in the appropriate cell culture plate at least 1 d prior to stimulation.
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bioRxiv - Neuroscience 2023Quote: ... diluted and seeded on Poly-D-lysine – Matrigel coated coverglasses in a 12 well plate at the concetration of 5×104 cells per well in the Patterning medium [N2B27 medium containing 1ng/mL GDNF (Peprotech), 2ng/ml BDNF (Pepotech) ...
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bioRxiv - Immunology 2023Quote: ... splenic NK cells were MACs enriched and cultured at 37°C and 5% CO2 in RPMI containing 300U rIL-2 (Peprotech), 50ng/ml rIL-15 (Peprotech ...
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bioRxiv - Genetics 2023Quote: ... and the cells were washed with phosphate buffered saline (PBS) and cultured in M231 SMDS medium supplemented with 5 ng/mL PDGF-BB (Peprotech) to provoke cell migration ...
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bioRxiv - Immunology 2023Quote: ... Cultures were changed on day 4 to new complete RPMI 1640 medium containing recombinant murine IL-5 (10 ng/ml, Peprotech) and further fed with IL-5 every other day between days 10 to 14 ...
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bioRxiv - Immunology 2024Quote: ... cells were thawed and rested in an 37°C / 5% CO2 incubator in the T cell medium with 50 U/mL rhIL-2 (Peprotech, Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2020Quote: ... NPCs were cultured for up to 5 passages on Matrigel with neural induction media supplemented with 40 ng/ml bFGF (Peprotech: (AF)100-18B) ...
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bioRxiv - Cell Biology 2022Quote: ... Activated cells were incubated for 5 days in the presence of the following: IL-12 (25 ng/ml, PeproTech, London, UK) and neutralizing antibody anti–IL-4 (1 μg/ml ...
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bioRxiv - Cancer Biology 2022Quote: Wild-type mouse colonic organoids were plated onto 24-well plates in Matrigel and allowed to culture for 5 days before treatment with recombinant murine INF-y (PeproTech, UK) at concentrations of 0.2 ...
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bioRxiv - Bioengineering 2023Quote: ... cells were differentiated at a concentration of 5 x 105 cells/mL with 100 nM phorbol 12-myristate 13-acetate (PMA, PeproTech, 1652981) in complete RPMI media for 24 hours followed by 3 days of resting in complete RPMI media ...
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bioRxiv - Immunology 2024Quote: ... A single round of co-sedimentation with retroviral stock was performed in medium containing 5% WEHI-3B-conditioned medium and 10 ng/mL IL-7 (Peprotech, USA). After transduction ...
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bioRxiv - Immunology 2021Quote: ... Cells were cultured at 1×106 cells/ml in complete RPMI-1640 supplemented with 20 ng/ml GM-CSF and 5 ng/ml IL-4 (PeproTech, Cranbury, NJ) for 7 days (Replaced 70% of medium on Day 2 and Day 5) ...
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bioRxiv - Systems Biology 2023Quote: B2B1 cells were seeded on tissue culture plastic (6-well to 15-cm plates) at 12,500 cells/cm2 in assay medium + 2% matrigel + 5 ng/ml EGF (Peprotech, AF-100-15) and refed every four days ...
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bioRxiv - Neuroscience 2023Quote: ... the embryoid bodies were centrifuged at 200 rcf for 5 min and resuspended in N2B27 supplemented with BDNF (10 µg/ml; PeproTech, 450-02), ascorbic acid ...
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bioRxiv - Pathology 2023Quote: ... or 5 ng/mL of recombinant murine interleukin (IL) −4 and −13 (IL-4, IL-13) (ILs) (PeproTech 214-14, 210-13), were added to the serum-free media ...
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bioRxiv - Cancer Biology 2024Quote: ... according to the manufacturer’s instructions (20 μL of beads per 1 million T-cells) for 5 days and grown in the presence of recombinant interleukin-7 (PeproTech, 200-07) and interleukin-15 (PeproTech ...
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bioRxiv - Cancer Biology 2024Quote: ... according to the manufacturer’s instructions (20 μL of beads per 1 million T-cells) for 5 days and grown in the presence of recombinant interleukin-7 (PeproTech, 200-07) and interleukin-15 (PeproTech ...
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bioRxiv - Biophysics 2023Quote: ... the cells were stimulated at 37 °C for 15 min with 2 mL of 5 nM InlB321 variant (Table S1) or 1 nM HGF (#100-39H, PeproTech, Hamburg, Germany) in serum-free DMEM-F12 ...
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bioRxiv - Immunology 2023Quote: ... A total of 1.5×106/well CD14+ monocytes were seeded in a 12-well plate with 50 ng/mL of hM-CSF (Peprotech, 300-25). On day 6 ...
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bioRxiv - Cancer Biology 2021Quote: ... or 2) subsequently stimulated with 5 μg/kg T cell chemokine – C-X-C motif chemokine ligand 10 (CXCL10, PeproTech, Rocky Hill, USA) (23) ...
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bioRxiv - Cancer Biology 2023Quote: ... For cytokine treatment cells were stimulated with either 5 ng/mL of TGFβ1 or 50 ng/mL of BMP6 (both from PeproTech, Rocky Hill, NJ, US). Where indicated ...
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bioRxiv - Cell Biology 2020Quote: ... They were grown under standard conditions at 37°C and 5% CO2 with NeuroCult basal media (STEMCELL, Vancouver, BC, CA) with EGF (50 ng/ml, PeproTech, Inc., Rocky Hill, NJ, USA), FGF (100 ng/ml ...
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bioRxiv - Immunology 2021Quote: ... Sorted cells were seeded in 10 cm tissue-culture dishes of 80 – 90% confluent OP9 cells containing 5 ng/ml Flt-3L (R&D, Catalogue no.-427-FL) and 1 ng/ml IL-7 (PeproTech, Catalogue no.- 217-17-10). For T-cell development assay ...
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bioRxiv - Developmental Biology 2021Quote: ... the SC solution was centrifuged at 300 g for 5 minutes to remove the supernatant and SFM containing 50 μg/ml stem cell factor (PeproTech, 300-07, Cranbury, NJ, USA), 100 μg/ml IL-3 (PeproTech ...
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bioRxiv - Cancer Biology 2023Quote: ... Activated T-cells were kept in medium supplemented with IL-7 and IL-15 (#200-07 and #200-15, PeproTech, 10 and 5 ng/mL, respectively). Gamma-retroviral transduction was facilitated by RetroNectin (#T100B ...
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bioRxiv - Immunology 2024Quote: ... at 0,5 μg/mL each, with IFN-γ (R&D, cat #285-IF-100) at 1000 IU/mL or with TNF-α (Peprotech, cat #AF- 300-01A) at 1ng/mL ...
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bioRxiv - Cancer Biology 2022Quote: ... Equal amounts of conditioned media were measured using a VEGF-A kit (Peprotech, Rock Hill, NJ) that recognizes human VEGF-A (hVEGF-A ...
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bioRxiv - Cell Biology 2021Quote: ... Each serum was assayed for murine IFN-y in duplicate using an enzyme immunoassay kit (Peprotech, BGK01580).
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bioRxiv - Immunology 2023Quote: ... fixed cells were permeabilised with the Transcription Factor staining kit (eBioscience) for 20min at room temperature and stained for 45min with rabbit anti-mouse RELMα (Peprotech) and biotinylated goat anti-mouse Ym1 (R&D) ...