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Citations for Peprotech :
1351 - 1397 of 1397 citations for Recombinant Rat Il6st His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2024Quote: ... Exploration of BMP2 effect on HSJD-DIPG-012 and HSJD-DIPG-014 spheroids was done using 10 ng/mL of human recombinant BMP2 (Peprotech, 120-02C) before protein quantification by western blot.
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bioRxiv - Biochemistry 2024Quote: ... Sigma-Aldrich P6407) in OPC proliferation media (DMEM-Sato) supplemented with 10 ng/ml recombinant human ciliary neurotropic factor (CNTF; Peprotech 450-13), 10 µM forskolin (Sigma-Aldrich F6886) ...
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bioRxiv - Immunology 2024Quote: ... pH 7.34 for a short stimulation of 30 min) or in a CBMC medium supplemented with recombinant h-SCF (100 ng/mL, Gibco, PeproTech, #300-07) (for a longer stimulation) ...
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bioRxiv - Neuroscience 2024Quote: MRMT-1 cells were treated with DsiRNA-containing lipid nanoparticles (5 µg/ml) containing DID dye in the presence or absence of recombinant 10 µg/ml ApoE4 (PeproTech #350-04) for 24 h ...
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bioRxiv - Physiology 2019Quote: ... Cells were then serum starved in DMEM culture media with 2% BGS and the conditioned culture was supplemented with 200ng/ml of murine IL-10 recombinant protein (Peprotech, Rocky Hill, NJ). After 48 hours ...
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bioRxiv - Molecular Biology 2019Quote: ... 10 weeks old) were subcutaneously injected into the groin regions with 400 μL Matrigel containing recombinant mouse basic FGF (PeproTech, 250 ng/mL) and heparin (Sigma ...
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bioRxiv - Bioengineering 2021Quote: ... supplemented with 50 ng/mL FGF2 and 30 ng/mL animal-free recombinant human Bone Morphogenic Protein 4 (BMP4, Peprotech, AF-120-05ET) for 24 hours ...
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bioRxiv - Cancer Biology 2022Quote: ... Cells were then cultured on untreated plates for 7 days adding 10 ng/ml recombinant murine M-CSF (Peprotech, AF-315-02-100) at day of isolation ...
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bioRxiv - Microbiology 2021Quote: ... control and CSP-stable transfected HepG2 cells were pre-treated with 1 U/mL recombinant human IFN-γ (Peprotech, Rocky Hill, NJ, USA) or equivoluminal medium for 6 h ...
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bioRxiv - Immunology 2020Quote: ... 1640 Medium supplemented with 10% fetal bovine serum (35-076-CV; Coring, New York, USA) and 20 ng/μL Recombinant Human GM-CSF (300-03; Peprotech, Rocky Hill, USA).BEAS-2B cells (CRL-9609 ...
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bioRxiv - Immunology 2019Quote: ... the suspension cells were removed and culture in a new flask along with 10ng/ml recombinant murine IL-3 (Peprotech, Rocky Hill, NJ). Cells were passaged twice per week ...
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bioRxiv - Developmental Biology 2020Quote: ... LepRb-Cre::tdTom and GCG-Cre::SynTom mice received intraperitoneal (i.p.) injections of recombinant mouse leptin (10mg/kg body weight; Peprotech Inc., Rocky Hill, NJ), or vehicle (0.9% sterile saline) ...
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bioRxiv - Immunology 2019Quote: ... 50μM 2-ME and 100 ng/ml of recombinant murine fms-like tyrosine kinase-3 ligand (Flt3L, PeproTech Inc., Rocky Hill, NJ, USA) for 7days ...
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bioRxiv - Molecular Biology 2021Quote: ... BMDM and AM were cultured in Dulbeccòs Modified Eagle’s Medium (DMEM) + 10% heat-inactivated fetal bovine serum (FBS) supplemented with 20 ng/ml recombinant murine M-CSF (PeproTech, Rocky Hill, NJ) and 20 ng/ml recombinant murine RANKL (PeproTech) ...
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bioRxiv - Immunology 2020Quote: ... Cells were treated with Mouse active perforin (#APB317Mu01, Cloud-Clone Corp., Katy, TX) and recombinant mouse Granzyme B (#140-03, PeproTech, Rocky Hill, NJ) for 4 to 6 hours in indi cated concentrations following manufacturer’s recommendations.
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bioRxiv - Immunology 2021Quote: ... Human monocyte-derived macrophages (HMDMs) were differentiated from purified CD14+ monocytes in the presence of recombinant human M-CSF (50 ng/ml; PeproTech, Cranbury, NJ, USA) for 6 days in RPMI 1640 medium supplemented with 10% FBS ...
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bioRxiv - Immunology 2020Quote: ... Isolated PBMC were washed with PBS and resuspended by RPMI-1640 which supplemented 50ng/ml recombinant human macrophage colony-stimulating factor (M-CSF) (AF-300-25-500, PeproTech, Cranbury, NJ, USA), then cultured at 37°C in 5% CO2 for 5 days to differentiate into human monocyte derived macrophages (hMDMs ...
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bioRxiv - Immunology 2021Quote: ... were plated with the irradiated 40LB cells in R10 media with 1 ng/mL recombinant mouse interleukin-4 (rmIL-4; Peprotech, East Windsor, NJ). On day 4 ...
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bioRxiv - Immunology 2022Quote: ... The following cytokines were added at the indicated concentrations for murine γδT cells: recombinant murine IL-12 (10ng/ml) (Peprotech, Rocky Hill, NJ), IL-15 (50ng/ml ...
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bioRxiv - Immunology 2022Quote: ... Cells were differentiated to macrophages in RPMI x1640 supplemented with 10% FBS containing 10 ng/ml of recombinant human GM-CSF (Peprotech, New Jersey, US) for 7 days ...
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bioRxiv - Bioengineering 2022Quote: ... MDTs were exposed to a neutral culture medium or to a 20 ng/ml of TNF solution (Recombinant TNF alpha human; 300-01A, PeproTech, Thermo Fisher Scientific) in culture medium for either 30 minutes or 240 minutes ...
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bioRxiv - Developmental Biology 2024Quote: ... proteins were supplemented in Maturation media by dissolving Matrigel at 1% (v/v) containing human recombinant brain-derived neurotrophic factor (BDNF; PeproTech, AF-450-02). The aggregated cells were referred to as cerebral organoids from this stage onward and were allowed to further develop in maturation media until the experimental endpoints ...
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bioRxiv - Genomics 2024Quote: ... For M2 polarization macrophages were cultured in macrophage media supplemented with 20 ng/ml recombinant human IL-4 (PeproTech, catalog no. 200-04). Cells were harvested after 24 ...
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bioRxiv - Immunology 2022Quote: ... or CD11b+ enriched single cell suspensions of B16F10 tumors were cultured in the presence of 50 ng/mL recombinant M-CSF (Peprotech, Cranbury, NJ, USA) in RPMI (Wellgene ...
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bioRxiv - Biochemistry 2023Quote: ... 40,000 cells were seeded per cavity of a 96-well plate and incubated for 20 h with serial dilutions (1000 – 0.076 pM) of the fusion protein Af2-IL-2 and the reference recombinant human IL-2 (PeproTech, Rocky Hill NJ, USA), respectively ...
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bioRxiv - Immunology 2023Quote: PBMC from healthy donor buffy coats were isolated by Ficoll density centrifugation and rested overnight in 10 ng/mL recombinant human IL-15 (Peprotech, cat #200-15) at 2*106 cells/mL cell culture media (RPMI-1640 ...
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bioRxiv - Immunology 2023Quote: ... iPSCs were differentiated to macrophages[73] and stimulated for 6h and 24h with 10 ng/mL recombinant human interleukin-10 (Peprotech, 200-10-2), 10 ng/mL recombinant human interferon-b (Peprotech ...
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bioRxiv - Immunology 2023Quote: Transplant recipient and healthy donor cryopreserved PBMC were thawed and recovered overnight in 10 ng/mL recombinant human IL-15 (Peprotech, cat #200-15) at 2*106 cells/mL cell culture media (RPMI-1640 ...
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bioRxiv - Immunology 2021Quote: ... cells isolated from the femur and tibia were cultured at 1 × 106 cells/ml in 10 ml of complete DMEM (cDMEM) supplemented with 4% recombinant mouse GM-CSF (Peprotech, Rocky Hill, NJ, USA) at 37°C in 7.5% CO2 ...
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bioRxiv - Bioengineering 2021Quote: PTMs were differentiated from hiPSCs using the above protocol for hiPSC-derived Leydig cells with the modification that PDGF-AA is replaced by human recombinant Platelet-Derived Growth Factor-BB (PDGF-BB, Peprotech, 100-14B, Figure 1B). On day 25 the cells were expanded on PLL-coated plates in DMEM/F12 ...
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bioRxiv - Neuroscience 2020Quote: ... and 1% insulin transferrin selenium (Cat N° 41400045) in IMDM (Cat N° 12440053) supplemented with 5 ng/ml recombinant human basic fibroblast growth factor (bFGF) (Peprotech, Cat N° 100-18B). Medium was changed every 4 days ...
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bioRxiv - Immunology 2022Quote: ... Cells were incubated for 3 hours at 37°C in RPMI media supplemented with or without recombinant mouse IL-21 (20 ng/mL, Peprotech cat. 210-21-100). Cells were stimulated with either anti-Igκ and anti-Igλ or anti-CD40 ...
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bioRxiv - Immunology 2022Quote: ... hCD34+ cells were either kept in culture at 37°C in RPMI medium with human recombinant Stem Cell Factor (SCF) at 50 ng/ml (PeproTech, Rocky Hill, NJ, USA), human recombinant thrombopoietin (TPO ...
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bioRxiv - Immunology 2020Quote: ... extracellular Cn were removed by washing 2X with PBS and the macrophages were cultured for a further 24 h in fresh growth media containing either IFNγ to maintain the M1 polarization state or 100 ng/mL recombinant interleukin-4 (IL-4; Sigma-Aldrich, St. Louis, MO, and Peprotech, Rocky Hill, NJ) to promote repolarization to the M2 state ...
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bioRxiv - Microbiology 2021Quote: ... 2.5×105 HUVECs were plated in wells of 6-well plates and stimulated overnight with 20 ng.ml−1 human recombinant TNFα (PeproTech/Tebu Bio, #300-01A-A) or infected at MOI=200 with Neisseria meningitidis for 30 min ...
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bioRxiv - Biophysics 2023Quote: ... we tuned the cellular contractility by replacing the medium with a new one containing recombinant human TGF-β (2.5 ng/mL, 100-21, PeproTech, Rocky Hill, NJ, USA), to promote cellular contractility ...
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bioRxiv - Cell Biology 2023Quote: ... cells were plated in 1% FBS- containing DMEM for 72 hrs with or without 4ng/mL human recombinant TGF-β (Peprotech; cat #100-21).
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bioRxiv - Cancer Biology 2023Quote: ... the FGF and EGF mitogens in the media were replaced by 100 ng/ml of BMP4 (Recombinant human BMP-4, PeproTech, Cat# AF-120-05ET) (quiescence media) ...
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bioRxiv - Biochemistry 2019Quote: ... The cells were subject to cytokines stimulation in serum free medium containing 10 ng/ml rat IFN-γ (Peprotech, #400-20) and 10 ng/ml rat TNF-α (Peprotech ...
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bioRxiv - Cancer Biology 2021Quote: ... and the next day grown in the presence or absence of 100 IU/mL recombinant murine IFN-γ (PeproTech, Cranbury, NJ, Cat. No. 315-05) for 24 hours ...
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bioRxiv - Microbiology 2023Quote: Confluent monolayers of MRC-5 and LEC were treated with 25 ng/mL recombinant human IFN-β and γ (Peprotech #300-02BC and #300-02) in the culture media ...
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bioRxiv - Immunology 2022Quote: ... 235 μL of complete DMEM supplemented with 1 % rat serum with or without chemotactic stimuli 30 ng/mL CXCL10 (PeproTech, 400-33) and CCL5 (PeproTech ...
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bioRxiv - Cell Biology 2020Quote: ... and resuspended in macrophage complete medium (DMEM/F12, 10% FBS, 1% penicillin/streptomycin, 100 U/ml recombinant murine M-CSF; Peprotech, Rocky Kill, NJ; Cat# 315-02). 5 ml of macrophage complete medium was added on day 3 ...
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bioRxiv - Bioengineering 2020Quote: ... and resuspended in macrophage complete medium (DMEM/F12, 10% FBS, 1% pen/strep, 100 U/ml recombinant murine M-CSF; Peprotech, Rocky Kill, NJ; Cat# 315-02). 5 ml of macrophage complete medium was added on day 3 ...
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bioRxiv - Cancer Biology 2019Quote: ... The methods of low or high-dose IFN-γ treatment for 6 days in A549 and H460 cells were as follows: recombinant human IFN-γ (Peprotech, Rocky Hill, NJ, USA) were added and cultured for 2 days ...
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bioRxiv - Immunology 2021Quote: ... mice were administrated intratumorally (i.t.) with 50 μl of PBS (left flank) or murine recombinant GPR15L procured from PeproTech (right flank, 2.5 μg in PBS/injection) 3 times per week for 2 weeks ...
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bioRxiv - Neuroscience 2022Quote: ... 10k primary rat microglia were added to 20k DIV12 primary hippocampal neurons with 40 ng/mL of rat macrophage colony stimulating factor (MCSF) (Peprotech 400-28-100UG). At the time of plating ...