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Citations for Peprotech :
1351 - 1400 of 1539 citations for Caspase 7 Rabbit Recombinant mAb since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: ... Th2 cells were generated by activating naïve CD4+ T cells with 1 μg/mL anti-CD3ε/3 μg/mL anti-CD28 for 3 days in complete T cell medium supplemented with 250U recombinant IL-4 (PeproTech), 6 μg/mL anti-IL-12 (BioXcell) ...
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bioRxiv - Molecular Biology 2024Quote: SP2/O-Ag14 myeloma and primary clones of hybridomas were cultured in GIT medium (catalog no. 63725715, Wako) supplemented with recombinant human interleukin-6 (IL-6) (catalog no. 20006, PeproTech). Cells were cultured in a humidified atmosphere containing 5% CO2 at 37°C and sub-cultured every day ...
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bioRxiv - Immunology 2024Quote: ... Cells were expanded for at least 1 week in the presence of IL-3 and 30 ng/mL recombinant SCF (Peprotech) to obtain peritoneal cavity-derived mast cells (PCMCs) ...
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bioRxiv - Developmental Biology 2024Quote: ... expanded neural progenitors were re-plated at a density of 3×104 cells/cm2 in expansion media and 24 hours later switched to N2B27 supplemented with 10 ng/mL human recombinant CNTF (Peprotech) and 10 ng/mL human recombinant BMP4 (Peprotech ...
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bioRxiv - Cell Biology 2024Quote: ... Recombinant basic fibroblasts growth factor (bFGF; 100-18B) and epidermal growth factor (EGF; AF-100-15) were purchased from PeproTech Inc..
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bioRxiv - Immunology 2024Quote: ... splenocytes were restimulated for 5 hours at 37°C with 1 μg/ml of MHC class I-restricted LCMV peptide gp33-41 in the presence of 2 ng/ml recombinant human IL-2 (Peprotech), 10% fetal bovine serum (premium FBS ...
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bioRxiv - Genomics 2024Quote: ... macrophages were cultured in macrophage media supplemented with 20 ng/ml human recombinant IFN-γ (PeproTech, catalog no. 300-02) and 25 ng/ml LPS (Invivogen ...
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bioRxiv - Immunology 2024Quote: ... The medium was also supplemented with 10 U/ml of DNaseI and 2 ng/mL of recombinant IL-15 (Peprotech). The cell suspension was transferred to a T25 flask (Corning ...
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bioRxiv - Immunology 2024Quote: ... Approximately 5 × 106 NK cells were co-cultured with 1 × 107 of the modified and irradiated K562 feeder cells in 40 mL of Complete NK MACS medium supplemented with 100 U/mL of recombinant IL-2 (Peprotech) in a T75 flask (Corning) ...
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bioRxiv - Immunology 2022Quote: ... splenocytes from control and Ncr1cre Mycfl/fl mice were left unstimulated or stimulated with 50 ng/mL recombinant mouse IL-15 (PeproTech) for 40 min at 37 °C and analyzed for intracellular protein phosphorylation by flow cytometry ...
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bioRxiv - Pathology 2023Quote: ... Bone marrow was collected and cultured in RPMI-1640 complete medium (CM) containing 20ng/ml recombinant mouse GM-CSF (Peprotech). Subsequently ...
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bioRxiv - Immunology 2023Quote: ... supplemented with 5% heat-inactivated fetal bovine serum (Biosera FB-1001) and 50ng/ml recombinant M-CSF (Peprotech 300-25). Differentiated macrophages were plated at a density of 1 x 106ml in the appropriate cell culture plate at least 1 d prior to stimulation.
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bioRxiv - Physiology 2023Quote: ... protein from transfected THP-1-derived macrophages and human cardiac fibroblasts treated with macrophage supernatant or with recombinant human HB-EGF (100 ng/ml, 30min, #100-47, PeproTech) was isolated ...
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bioRxiv - Neuroscience 2023Quote: ... Cells were replated onto glass coverslips at day 12 of the differentiation in N2/B27 medium supplemented with four recombinant growth factors at 25ng/ml (BDNF; ThermoFisher, NT3, NGF, GDNF; Peprotech). CHIR90221 was included for 4 further days ...
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bioRxiv - Microbiology 2023Quote: ... 1 x 106 BLaER1 cells per well of a 6-well tissue culture plate were treated with 10 ng/ml human recombinant M-CSF and IL-3 (PeproTech) and 100 nM β-estradiol (Sigma-Aldrich ...
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bioRxiv - Immunology 2023Quote: Primary Keratinocytes from three donors were seeded in 24-well plates and stimulated with KGM Media supplemented either with 0.1 ng/ml or 10 ng/ml of recombinant human IFN-γ (PeproTech), or infected with T ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells were passed through 70-micron cell strainer and subsequently cultured 10mL complete RPMI media supplemented with 20 ng/mL of recombinant murine M-CSF (576406; BioLegend, 315-02 PeproTech). On day 3 ...
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bioRxiv - Cell Biology 2023Quote: ... cells were either given fresh MC medium or cultured in basal MC medium (1% FBS) or in basal MC medium supplemented with 50ng/mL recombinant human VEGF-A165 (100-20; Peprotech) and culture for another 3 days.
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bioRxiv - Bioengineering 2023Quote: ... At Day 11 the cytokine composition of the media was changed to 10ng/μl of bFGF and human recombinant Hepatocyte Growth Factor (HGF) at 10ng/μl (Peprotech). From Day 15 onwards ...
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bioRxiv - Immunology 2023Quote: ... cells were washed and then expanded and differentiated in complete IMDM (as above) containing 20 ng/mL recombinant human IL-2 (Peprotech) for a further 4 days ...
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bioRxiv - Immunology 2023Quote: ... Bone marrow-derived dendritic cells (BMDCs) were generated in tissue culture using recombinant GM-CSF (Peprotech, Cat. AF-315-03). Class B CpG 1826 oligonucleotide was synthesized by Integrated DNA Technologies ...
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bioRxiv - Cancer Biology 2023Quote: ... Differentiation of BM cells into DCs was carried out in low attachment 10 mm cell culture dish in presence of bone marrow-differentiation media in presence of recombinant murine Granulocyte-Macrophage Colony-Stimulating Factor (GM-CSF) (Cat. 315-03, Peprotech) for 48 a ...
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bioRxiv - Cancer Biology 2023Quote: ... These tumoroids were then cultured in BEN selection media (basal medium with mouse recombinant EGF (50 ng/ml) and Noggin (100 ng/ml) (PeproTech) without R-spondin ...
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bioRxiv - Neuroscience 2023Quote: ... Astrocytes were then cultured with and without 14 x 103 LNCs/well and treated with 10 ng/mL recombinant murine IFNγ (Peprotech), 100 nM PD-1/PD-L1 inhibitor (Thomas Scientific ...
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bioRxiv - Immunology 2023Quote: ... Cultures were changed on day 4 to new complete RPMI 1640 medium containing recombinant murine IL-5 (10 ng/ml, Peprotech) and further fed with IL-5 every other day between days 10 to 14 ...
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bioRxiv - Immunology 2024Quote: ... For Th1 polarization T lymphocytes were cultured with 5 ng/ml of recombinant murine IL-12 (Peprotech, catalog 210-12). For stimulation of preactivated T lymphocytes with type I IFN ...
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bioRxiv - Immunology 2024Quote: ... Cells were plated in DMEM containing 10% v/v FBS and 10 ng/ml recombinant M-CSF (Peprotech #315-02). Cells were plated at a concentration of ∼150,000 cells per cm2 on non- treated Petri plates ...
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bioRxiv - Immunology 2024Quote: ... Isolated cells were assayed immediately or cultured overnight in StemSpan SFEM (STEMCELL) with 50 ng/mL recombinant human stem cell factor (PeproTech), 10-6 M dexamethasone (Sigma) ...
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bioRxiv - Developmental Biology 2024Quote: ... On day 11 the cytokine composition of the media was changed to 10ng/ml of bFGF and human recombinant Hepatocyte Growth Factor (HGF) at 10ng/ml (Peprotech). From day 15 onwards ...
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bioRxiv - Immunology 2024Quote: ... Bone marrow was stimulated with recombinant murine IL-3 (10 ng/mL) and IL-6 (10 ng/mL) (Peprotech, 21616) for 48 hours ...
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bioRxiv - Immunology 2024Quote: ... 1×106 differentiated iRBCs or undifferentiated HUDEP-2 cells were stimulated with 50 ng/mL recombinant human interferon-gamma (PeproTech) for 6 hours ...
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bioRxiv - Cell Biology 2024Quote: ... 5 µM A83-01 without EGF (HOME0) or with 0.1-50 ng/mL human recombinant EGF (Peprotech, Cranbury, NJ, USA) (HOME0.1 ...
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bioRxiv - Immunology 2024Quote: ... filtered and cultured for 7 days in complete RPMI medium (RPMI-1640 medium containing L-glutamine plus 10% fetal calf serum) containing 20ng/mL recombinant GM-CSF (Peprotech).
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bioRxiv - Immunology 2023Quote: ... Unconjugated polyclonal rabbit anti-RELMα (Peprotech) was detected with goat anti-rabbit AF647 plus (ThermoFisher) ...
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bioRxiv - Immunology 2023Quote: ... rabbit anti-RELMα (Peprotech, 500-P214), and mouse anti-GAPDH (Santa Cruz ...
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bioRxiv - Immunology 2021Quote: ... with 2% FBS (HyClone Thermo Fischer Scientific) for 15 minutes with or without murine IL-7 (50 ng/mL, PeproTech). Following stimuli ...
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bioRxiv - Systems Biology 2019Quote: ... All in vitro T cell generation cultures were supplemented with 5ng/mL Flt3-L and 5 ng/mL IL-7 (Peprotech), and were sorted after 6 or 7 total days of culture before transducing with retroviral vectors or treating with small molecule inhibitors ...
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bioRxiv - Cancer Biology 2022Quote: ... mAb in 200ul of T cell media supplemented with 5 ng/ml of IL-7 and 30 U/ml of IL-2 (PeproTech) for 4 and 7 days ...
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bioRxiv - Immunology 2020Quote: ... 5 ng/ml of IL-2 was added to the culture and memory T cells were supplemented with 5 ng/ml IL-7 and IL-15 (all Peprotech). Cells were activated in a reverse time-course so that their total time in culture was identical and so that all cells would finish their indicated activation period at the same day to enable extracellular flux analysis ...
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bioRxiv - Cancer Biology 2021Quote: ... bone marrow cells were obtained from 8-10 weeks old BALB/c mice and cultured for 7 days in DMEM supplemented with 10% FBS and 20 μg/ml macrophage-colony stimulating factor (M-CSF, Peprotech). All medium contained 50 U/ml penicillin and 50 μg/ml streptomycin (Sigma-Aldrich).
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bioRxiv - Immunology 2020Quote: ... These cells were cultured for 7 d in DMEM cell culture medium containing 40 ng/ml macrophage colony-stimulating factor (M-CSF) (PeproTech). For the generation of bone marrow-derived dendritic cells ...
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bioRxiv - Immunology 2021Quote: Bone marrow cells were isolated from C57BL/6J mouse tibia and femur and then cultured for 7–9 days in 10% FBS DMEM containing mouse GM-CSF (50 ng/mL, Peprotech).
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bioRxiv - Immunology 2021Quote: ... peptide-reactive T-cell lines from day 925 and 945 were expanded in culture for two weeks with IL-7 and IL-15 (5ng/mL; Peprotech) and cultured for 24h with irradiated (100Gy ...
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bioRxiv - Cancer Biology 2022Quote: ... Enriched cells were taken into culture in T cell medium (TCM, see Methods – primary human material and cell lines) supplemented with 5 ng/mL IL-7 (Peprotech), 5 ng/mL IL-15 (Peprotech) ...
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bioRxiv - Immunology 2022Quote: ... The culture conditions used in this study were as follows: HSP condition – cell cultured with IL-15 and -7 (10 μg/ml, PeproTech); HSP+TCR condition – cells cultured with IL-15 and -7 and activated by anti-CD3 (5 μg/ml ...
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bioRxiv - Immunology 2023Quote: Isolated CD8+ T cells from OT-I mice were maintained in the naive status after CRISPR-Cas9 gene targeting by resting them overnight in complete medium added with IL-7 (Peprotech) at 10 ng/ml ...
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bioRxiv - Immunology 2023Quote: ... 4-6 x 106 P14 CD8 T cells were plated in 6 well plates and pre-cultured in IL-7 (10ng/ml, PeproTech) at 37°C ...
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bioRxiv - Immunology 2023Quote: ... T cells were re-plated with fresh medium containing 10 ng/ml rhIL-2 or a combination of 10 ng/ml IL-7 and IL-15 (all Peprotech) and differentiated at a density of 5×105 cells/ml for additional 4 days ...
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bioRxiv - Developmental Biology 2024Quote: ... All in vitro T cell generation cultures were supplemented with 5ng/mL Flt3-L and 5 ng/mL IL-7 (Peprotech). Experiments involving in vitro generation of NK and ILC populations (Figures 3 ...
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bioRxiv - Immunology 2021Quote: ... when the cells were stimulated with 1000 U/ml of rhIL-2 and the combination of 10 ng/ml of recombinant murine (rm) IL-12 (210-12, PeproTech Inc) and 40 ng/ml of rm IL-15 (210-15 ...