Labshake search
Citations for Peprotech :
1301 - 1350 of 1355 citations for Rat UDP glucuronosyltransferase 1 2 UGT1A2 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2024Quote: ... Cells were expanded for at least 1 week in the presence of IL-3 and 30 ng/mL recombinant SCF (Peprotech) to obtain peritoneal cavity-derived mast cells (PCMCs) ...
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bioRxiv - Developmental Biology 2024Quote: ... cells were replated as single cells onto poly-D-lysine-laminin-coated plates at 1×106 cells/cm2 for astrogenic switch and progenitor expansion in N2B27 media supplemented with 10 ng/mL FGF2 (Peprotech) and 10 ng/mL Human EGF (Peprotech ...
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bioRxiv - Immunology 2024Quote: ... PBL, #12400-1; 200U IL-1α #211-11A, 200U IL-1β #211-11Β or both together at 200U total, PeproTech) to allow for pulmonary delivery one week (unless otherwise stated in the figure legends ...
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bioRxiv - Genomics 2023Quote: ... and 3 x 106 cells were plated to grow in suspension in 90 mm bacterial petri dishes containing N2B27 media supplemented with 1 μg/ml doxycycline and 10 ng/ml EGF and FGF (Peprotech). Embryoid-body-like aggregates were collected by mild centrifugation (100 g for 2 min ...
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bioRxiv - Cell Biology 2023Quote: ... cells were either given fresh MC medium or cultured in basal MC medium (1% FBS) or in basal MC medium supplemented with 50ng/mL recombinant human VEGF-A165 (100-20; Peprotech) and culture for another 3 days.
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bioRxiv - Biochemistry 2022Quote: ... For dot blot analysis of BMP-7 in conditioned media from co-culture assays 5 µl were applied to a nitrocellulose membrane and BMP-7 was detected using a rabbit pAb anti-BMP-7 GF at 1:2000 (#500-P198 B, Peprotech). For dot blot analysis in conditioned media of 3D organotypic co-cultures ...
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bioRxiv - Immunology 2023Quote: ... HeLa-sia) at a ratio of 1:100 in complete RPMI medium supplemented with 10 ng/mL GM-CSF (PeproTech). Cancer cells were seeded at an initial concentration of 1×10e4 cells/mL and the same amount of medium supplemented with GM-CSF was added at day 4 of the experiment ...
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bioRxiv - Neuroscience 2023Quote: ... with the addition of growth factors (10 ng/mL BDNF, Peprotech #450-02; 10 ng/mL GDNF, Peprotech #450-10; 1 ng/mL TGF-β1, Peprotech #100-21C ...
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bioRxiv - Microbiology 2023Quote: ... Monocyte-derived DCs were differentiated by stimulating THP-1 cells with human GMCSF (1500 IU/ml) and IL-4 (1500 IU/ml) (Peprotech) for 5 days with half the medium replaced at day 3 ...
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bioRxiv - Immunology 2024Quote: ... The isolated pDCs (85% purity) were maintained overnight in the presence of IL-3 (1 ng/ml) (PeproTech, London, UK) before stimulation.
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bioRxiv - Neuroscience 2024Quote: ... 0.1% Tween-20 (TBS-T) and bound SDF-1α-CXCL12 chemokine was detected with anti-murine SDF-1α-CXCL12 antibody (diluted 1:2000, Peprotech) followed by an HRP-conjugated anti-rabbit antibody (diluted 1:16000 ...
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bioRxiv - Immunology 2024Quote: ... PBMC were thawed and recovered in complete RPMI overnight without exogenous cytokines before being stimulated with either 1 ng/mL of IL-15 (Peprotech) or 1 ng/mL of IL-15+ 5 ng/mL TGF-β (Peprotech ...
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bioRxiv - Immunology 2024Quote: ... 3×105 CD14+ monocytes were seeded in a 12-well plate in 1 mL complete medium containing 20 ng/mL GM-CSF (#300-03; Peprotech) or 50 ng/mL M-CSF (#300-25 ...
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bioRxiv - Immunology 2021Quote: Both Lin-CD25+CD44- DN3 cells and Lin-CD25-CD44- DN4 cells were sorted from the thymi of LckCre+Zfp335+/+ and LckCre+Zfp335fl/fl mice and co-cultured with OP9-DL1 feeder cells in α-MEM medium in the presence of IL-7 (1 ng/ml, PeproTech) and Flt3-L (5 ng/ml ...
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bioRxiv - Neuroscience 2020Quote: Inflammation in primary glial cultures was induced by the addition of proinflammatory agents such as interleukin 1 beta (IL1β, 10 ng/mL; PeproTech, France) or lipopolysaccharide (LPS ...
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bioRxiv - Neuroscience 2019Quote: ... with 1% platelet-poor plasma-derived bovine serum (Biomedical Technologies 50-443-029) with 20 ng/mL bFGF (Peprotech 100-18B), and 10µM retinoic acid (RA ...
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bioRxiv - Immunology 2019Quote: ... bone marrow cells were plated at a density of 5×105 cells/mL in RPMI-1640 + 10% FCS + 100 U/mL penicillin/streptomycin + 1 mM β-mercaptoethanol (BM medium) with 20 ng/mL murine GM-CSF (Peprotech) and cultures in a humidified environment at 37°C and 5% CO2 ...
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Combination image flow cytometry reveals novel methods for isolating megakaryocyte progenitors cellsbioRxiv - Cell Biology 2019Quote: ... 1×107 cells were incubated in IMDM + 10% FBS supplemented with 50 ng/ml of recombinant murine TPO (PeproTech, #315-14) in a T75 flask at 37 °C in 5% CO2 for 72 h.
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bioRxiv - Immunology 2020Quote: ... Rinse solution was then aspirated and replaced with 1 mL of 2X concentrated EB medium (1X EB media: OXE8 medium supplemented with 50 ng/mL BMP4 (Peprotech, #PHC9534), 50 ng/mL VEGF (Peprotech ...
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bioRxiv - Cancer Biology 2024Quote: ... 5 x 105 splenocytes from single cell suspensions were incubated with increasing concentrations of recombinant mouse IFNγ (0, 0.1, 1, 10, 100 ng/mL) for 48 hours (Peprotech, 315-05). Cells were then harvested and incubated with a panel of antibodies containing anti-mouse CD19 (Biolegend,115504) ...
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bioRxiv - Neuroscience 2022Quote: ... in DMEM medium (+10 % FCS and 1% P/S) and stimulated with either 10 ng/ml TNF-α and 10 ng/ml IL-1β (both from Peprotech) or vehicle control ...
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bioRxiv - Biochemistry 2023Quote: ... 1% penicillin-streptomycin (P/S)) supplemented with 10-100 ng/mL recombinant murine fibroblast growth factor (Peprotech, Rocky Hill, NJ, USA). At passage 3 ...
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bioRxiv - Bioengineering 2023Quote: ... reprogramming was continued in E8 medium and from Day 6 to Day 18 in E7 medium (E6 medium, cat. A1516401, Thermo Fisher Scientific + 100ng ml-1 bFGF, cat. 100-18B, Peprotech). On Day 18 ...
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bioRxiv - Cancer Biology 2023Quote: ... Colonic organoids were cultured in organoid base medium further supplemented with 100 ng mL−1 murine WNT3A (mWNT3A, Peprotech 315-20), 50 ng mL−1 mEGF (Thermo PMG8041) ...
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bioRxiv - Cell Biology 2022Quote: ... The cell suspension was washed with PBS and plated in DMEM with 10 ng mL-1 M-CSF (Peprotech Inc, 315-02). Following 5 days of culture ...
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bioRxiv - Biochemistry 2019Quote: ... at 1 M cells per well in unconditioned medium with or without priming in 25 U IFN-γ (PeproTech, Rocky Hill, NJ). Bone-marrow-derived mast cells were prepared as described previously (55) ...
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bioRxiv - Bioengineering 2021Quote: ... HepatoSTIM™ medium was supplemented with 10 ng mL−1 of recombinant human TGF-β1 (100-21; PeproTech®, Rocky Hill, NJ), 100 ng mL−1 of recombinant human GDF-15 (120-28C ...
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bioRxiv - Immunology 2022Quote: ... in 500 μL of D10 medium in 24 well plates were stimulated for 7 days at 37°C with anti-mouse CD3 (1 µg/mL) and CD28 (0.5 mg/mL) antibodies plus mouse IL-6 (20 ng/mL) (Peprotech, Rocky Hill, NJ), and human TGF-β1 (2 ng/mL ...
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bioRxiv - Neuroscience 2022Quote: ... 100ng/mL cluster of differentiation 200 (CD200; Novoprotein # C31150UG) and100ng/mL fracktaline chemokine C-X3-C motif ligand 1 (CX3CL1; Peprotech # 300-31) (MBM + 5 GFs) ...
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bioRxiv - Cancer Biology 2020Quote: ... H1975 cells were cultured in stem cell culture media (Advanced DMEM/F12 [Thermo Fisher Scientific], 1% Glutamax, B-27 supplement [Thermo Fisher Scientific], 10 ng/mL recombinant human HB-EGF [PeproTech, Cranbury ...
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bioRxiv - Cancer Biology 2019Quote: ... using 5 μg/mL of the monoclonal anti-mouse IFN-γ antibody (eBioscience) for capture and 1 μg/mL of the polyclonal biotinylated anti-mouse IFN-γ antibody (PeproTech) for detection.
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bioRxiv - Neuroscience 2023Quote: ... L-glutamine and 1% Penicillin-Streptomycin (v/v) supplemented with CHIR-99021 (Stemgent, 0.5 μM) and brain derived neurotrophic factor (BDNF, PeproTech, 20 ng/ml). On the day in vitro 50 ...
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bioRxiv - Cancer Biology 2024Quote: ... according to the manufacturer’s instructions (20 μL of beads per 1 million T-cells) for 5 days and grown in the presence of recombinant interleukin-7 (PeproTech, 200-07) and interleukin-15 (PeproTech ...
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bioRxiv - Cell Biology 2023Quote: ... cells were washed 3 times with PBS and incubated in naïve dedifferentiation medium of mTeSR-1 supplemented with 12 ng/mL bFGF (Peprotech #AF-100-18B), 1 µM PD0325901 (MEKi ...
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bioRxiv - Bioengineering 2023Quote: ... OPCs were cultured using OPC maintenance/proliferation media (OMM) composed of N2B27 supplemented with 10ng/ml insulin-like growth factor (IGF-1; Peprotech, 100-11), 15ng/ml platelet-derived growth factor AA (PDGFaa ...
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bioRxiv - Cancer Biology 2024Quote: ... according to the manufacturer’s instructions (20 μL of beads per 1 million T-cells) for 5 days and grown in the presence of recombinant interleukin-7 (PeproTech, 200-07) and interleukin-15 (PeproTech ...
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bioRxiv - Bioengineering 2023Quote: ... M1-like MØs were obtained by culturing M0 MØs in RPMI media supplemented with 10pg/ml Lipopolysaccharides (MilliporeSigma, MO, USA) and 1 ng/ml human Interferon-gamma (Peprotech, NJ, USA). Protocol for generating M0 ...
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bioRxiv - Genomics 2024Quote: ... and the following day cells were fed with neural crest long-term medium (neural crest maintenance medium + 50 pg ml−1 BMP2 (PeproTech, 120-02) + 3 μM CHIR-99021 (Selleck Chemicals ...
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bioRxiv - Neuroscience 2024Quote: ... cells were resuspended in 320 μl of fresh DRG medium (F12, 10% hi-FBS, 1% Pen/Strep, 20 ng/ml mouse GDNF (Peprotech #450-44), 50 ng/ml mouse NGF (Peprotech #450-34) ...
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bioRxiv - Cell Biology 2021Quote: ... the transwells were dipped twice in PBS and transferred to a 6-well plate containing NSC medium (DMEM:F12 – glutamax, 1% pen/strep, 10 ng/mL EGF (Peprotech, AF-100-15-A), 10 ng/mL FGF (Peptrotech ...
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bioRxiv - Cancer Biology 2021Quote: ... The cells were incubated in RPMI1640 for THP-1 culture supplemented with human M-CSF at 20 ng/ml (cross-react with mouse, Peprotech, Cranbury, NJ, USA). After 2 days of culture ...
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bioRxiv - Immunology 2022Quote: ... On day 7 macrophages were harvested and then maintained in 20 ng ml−1 CSF-1 for subsequent experiments in which they were either maintained in medium without any further additions (M0) or with addition of 20 ng ml−1 IL-4 (Peprotech; MI(L-4)) ...
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bioRxiv - Microbiology 2021Quote: ... control and CSP-stable transfected HepG2 cells were pre-treated with 1 U/mL recombinant human IFN-γ (Peprotech, Rocky Hill, NJ, USA) or equivoluminal medium for 6 h ...
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bioRxiv - Immunology 2021Quote: ... were plated with the irradiated 40LB cells in R10 media with 1 ng/mL recombinant mouse interleukin-4 (rmIL-4; Peprotech, East Windsor, NJ). On day 4 ...
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bioRxiv - Immunology 2024Quote: Purified monocytes were plated at a concentration of 1 million cells/mL in 96 U-bottom wells and cultured in RPMI 1640 medium supplemented with 10% fetal bovine serum and 1% penicillin/streptomycin with 10 μg/mL of GM-CSF (Peprotech, cat #300-03). Cells were then cultured for 16-20h with or without stimulation.
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bioRxiv - Immunology 2024Quote: ... and cultured in DC media (15% FBS, 1% A/A, 50µM α-MTG, 20ng/mL granulocyte-macrophage colony stimulating factor (GM-CSF; Peprotech cat#: 315-03)
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bioRxiv - Developmental Biology 2024Quote: ... proteins were supplemented in Maturation media by dissolving Matrigel at 1% (v/v) containing human recombinant brain-derived neurotrophic factor (BDNF; PeproTech, AF-450-02). The aggregated cells were referred to as cerebral organoids from this stage onward and were allowed to further develop in maturation media until the experimental endpoints ...
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bioRxiv - Immunology 2021Quote: ... cells isolated from the femur and tibia were cultured at 1 × 106 cells/ml in 10 ml of complete DMEM (cDMEM) supplemented with 4% recombinant mouse GM-CSF (Peprotech, Rocky Hill, NJ, USA) at 37°C in 7.5% CO2 ...
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bioRxiv - Immunology 2020Quote: ... Cells were then plated into Petri dishes and cultured in BMDC differentiation medium (RPMI, 10% FBS, 1% penicillin-streptomycin, 1x sodium pyruvate, 1x β-mercaptethanol) supplemented with GM-CSF (Peprotech, Rocky Hill, NJ). Media was refreshed on day 2 ...
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bioRxiv - Cancer Biology 2021Quote: Primary mouse or NMR fibroblasts were seeded at 1 × 104 cells/well onto 24-well plates and stimulated with TNF-α (PeproTech; 50 ng/mL), z-VAD-fmk (Abcam ...