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Citations for Peprotech :
1251 - 1300 of 1331 citations for CTGF Human HEK293 Fc since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: ... with 500 nM SB431542 (Selleckchem, cat. no. S1067) and 20 ng/ml recombinant human (rh)Noggin (Peprotech, cat. no. 120-10C). On days 5-9 ...
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bioRxiv - Cancer Biology 2019Quote: ... 20ng/mL human recombinant epidermal growth factor (EGF) and 20ng/mL basic broblastic growth factor (bFGF; PeproTech Inc., Rocky Hill, NJ).
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bioRxiv - Immunology 2020Quote: ... containing 9 µg of encapsulated GM-CSF (Murine GM-CSF, Sanofi, USA; Human - Leukine® for pig and rabbit, Peprotech, USA) were combined with a 1 mL solution of captured antigen ...
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bioRxiv - Cell Biology 2020Quote: ... The monolayer was washed 3 times with HBSS to remove non-adherent cells and monocytes were differentiated for 3 days in RPMI media containing 20 ng/ml M-CSF and 20ng/ml recombinant human RANKL (PeproTech, UK). After 3 days of culture ...
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bioRxiv - Biophysics 2021Quote: ... after 24 h recombinant human IL-4 (20 ng/ml) and hSCF (100 ng/ml) (both Peprotech, Rocky Hill, NJ, USA) were added ...
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bioRxiv - Immunology 2022Quote: ... X-Vivo 15 media was supplemented with 100 ng/ml human granulocyte-macrophage colony-stimulating factor (GM-CSF) and 20 ng/ml human interleukin 4 (IL-4) (both cytokines obtained from Peprotech, Germany), and cells were cultured with 5% CO2 ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: HBD1 and LL-37 peptide release was quantified in supernatants from 25 day long-term cultured A549 cells using a human BD-1 Standard ABTS ELISA Development Kit (PeproTech, Germany) and LL-37 human ELISA kit (HycultBiotech ...
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bioRxiv - Cancer Biology 2019Quote: ... Cultures were maintained in complete RPMI with 200-300 U/ml human recombinant IL-2 (PeproTech, Rocky Hill, NJ #200-02) and media was refreshed every 3 days ...
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bioRxiv - Immunology 2021Quote: ... a 6-week-old C57BL/6 mouse was repeatedly immunized 5 times every other week subcutaneously (s.c.) into the flank with 10ug of human TNFR2 protein (Peprotech, 310-12)/20ug MPLA (Sigma ...
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bioRxiv - Cell Biology 2021Quote: ... Macrophages were then skewed towards the M2 phenotype using recombinant human IL-4 (20 ng/ml) (Peprotech, Cat#AF-200-04), IL-13 (20 ng/ml ...
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bioRxiv - Cancer Biology 2022Quote: ... blasts were pre-treated for 48 hours with Recombinant Human IFN-γ at a final concentration of 200 U/mL (cat#300-02 from PeproTech). Cells were cultured in IMDM supplemented with 1% L-glutamine (G) ...
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bioRxiv - Immunology 2022Quote: ... Whole mouse splenocytes and human PBMCs were plated at a density of 5×106 cells per well in a Cepallet W-type 24 well microplate (DIC, Japan) and cultured for 4 days with or without mouse/human M-CSF (Peprotech, USA). Surviving macrophages adhered to 24-well plates were detached by lowering the temperature on ice ...
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bioRxiv - Cancer Biology 2022Quote: ... PBMCs were harvested from K3EDTA anti-coagulated whole blood by density gradient centrifugation with Lymphoprep and plated in 12 well plates at 2-4 × 106 cells per well in 10% RPMI + 10 ng/mL human CSF (300-25, Peprotech, UK). Medium was replaced every 2-3 days until adherent populations of macrophages had established ...
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bioRxiv - Cell Biology 2024Quote: ... 0.5 μM of A83-01 (HY10432, MCE, USA) and 20 ng/mL of recombinant human epidermal growth factor (EGF; AF100-15, PeproTech, USA), 10% KSR and 1% NEAA ...
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bioRxiv - Developmental Biology 2023Quote: ... The differentiation medium was composed of modified N2B27 medium supplemented with 50 ng/ml recombinant human FGF4 (Peprotech, 100-31-25), 1 μg/ml heparin (Sigma ...
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bioRxiv - Cancer Biology 2023Quote: ... then 50 µL of 24 h co-culture supernatants or serial dilutions of Recombinant Human IFNγ standard (#300-02, Peprotech, USA) were added to the wells and incubated for 2 h at RT ...
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bioRxiv - Immunology 2023Quote: EndoC-βH1 cells and dispersed human islets were exposed or not to IFNα (2000 U/mL, PeproTech Inc., Rocky Hill, NJ), IFNγ (1000 U/mL ...
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bioRxiv - Immunology 2023Quote: ... Transfected cells were incubated for 24 hours before treatment with 50 ng/mL recombinant human IFN-γ (Peprotech; cat # 300-02) or cDMEM only control for 24 hours ...
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bioRxiv - Bioengineering 2023Quote: ... crosslinked following 10 min exposure to 100 mM CaCl2 and allowed to adsorb for 30 min with recombinant human vascular endothelial growth factor (rhVEGF 165, PeproTech, USA) at 100 μg mL−1 at 4 °C ...
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bioRxiv - Immunology 2024Quote: ... 50 μM 2-mercaptoethanol) with 100 ng/ml of human FMS-like tyrosine kinase 3 ligand (FLT3L, PeproTech, Cranbury, NJ, USA) for 7 days ...
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bioRxiv - Immunology 2024Quote: ... NBP2-35892), IFNω (Novusbio, NBP2-35893), IFNγ (Novusbio, NBP2-34992), IFNλ1 (Novusbio, NBP2-34996) and recombinant human TNFα (PeproTech, 300-01A).
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bioRxiv - Immunology 2024Quote: ... was used for sensitizing the cells in the presence of recombinant human IL-4 (Gibco, PeproTech, #200-04; 10 ng/mL) for three days ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were deprived of serum for 2h and stimulated with 2 ng/ml of recombinant human EGF (Peprotech; Catalog #AF-100-15) for 2 ...
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bioRxiv - Bioengineering 2021Quote: ... Media was serum-free TexMACS (Miltentyi Biotech 170-076-307) supplemented with recombinant human IL2 in concentrations specified by the DOE (Peprotech 200-02). Cell cultures were expanded for 14 days as counted from the time of initial seeding and activation ...
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bioRxiv - Cancer Biology 2019Quote: ... in 200µl of RPMI supplemented with 2% FBS and 5% Matrigel with or without the addition of human IFN-γ (PeproTech; #300-02), mouse IFN-γ (PeproTech ...
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bioRxiv - Bioengineering 2021Quote: ... HepatoSTIM™ medium was supplemented with 10 ng mL−1 of recombinant human TGF-β1 (100-21; PeproTech®, Rocky Hill, NJ), 100 ng mL−1 of recombinant human GDF-15 (120-28C ...
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bioRxiv - Microbiology 2020Quote: ... Frozen activated PBMC cells were thawed and resuspended in complete PBMC media containing RPMI supplemented with 21 ng/mL human IL-2 (Peprotech; #200-02), 10% fetal bovine serum and 1% penicillin-streptomycin ...
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bioRxiv - Microbiology 2020Quote: ... Pellets were resuspended in activation medium containing RPMI supplemented with 21 ng/mL human IL-2 (Peprotech, Rocky Hill, NJ; #200-02), 1.5 μg/mL PHA (Remel ...
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bioRxiv - Cancer Biology 2022Quote: ... the selected monocytes were cultured in complete Advanced-RMPI 1640 supplemented with either 20 ng/mL human GM-CSF (PeproTech, RockyHill, NJ), for M1 differentiation ...
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bioRxiv - Immunology 2022Quote: ... OT-I T cells were activated with 10-8 SIINFEKL or SIITFEKL (Cambridge Peptides) in the presence or absence of recombinant human TGFβ (Peprotech, 5ng/ml). Where indicated ...
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bioRxiv - Immunology 2020Quote: ... cells were washed and cultured for a further 4 days in media containing 20 ng/mL recombinant human IL-2 (Peprotech, London, UK). For analysis of effector cytokine production ...
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bioRxiv - Cancer Biology 2020Quote: ... H1975 cells were cultured in stem cell culture media (Advanced DMEM/F12 [Thermo Fisher Scientific], 1% Glutamax, B-27 supplement [Thermo Fisher Scientific], 10 ng/mL recombinant human HB-EGF [PeproTech, Cranbury ...
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bioRxiv - Pathology 2020Quote: ... HUVEC were plated at a density of 10,000 cells/96-well plate in EGM-2 supplemented with recombinant human TNF-α (300-01A, PeproTech EC Ltd), IL1-β (200-01B ...
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bioRxiv - Developmental Biology 2021Quote: ... LCDMYI supplementation was added as indicated at the following concentrations: 10 ng ml−1 recombinant human LIF (L, 10 ng ml−1; Peprotech, 300-05), CHIR99021 (C ...
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bioRxiv - Immunology 2020Quote: ... Alternately M2 MΦs were activated as follows: M2a MΦs from stimulation with 20 ng/mL recombinant human IL -4 (PeproTech; Rock Hill, NJ, USA) and 25 ng/mL recombinant human IL-13 (PeproTech ...
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bioRxiv - Biochemistry 2022Quote: ... cells were diluted to a density of 6e4/mL and 50 μL (3e3 cells) were added to 50 μL of media containing 2x of a serial dilution of human CSF3 (PeproTech 300-23) in a 96-well plate ...
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bioRxiv - Bioengineering 2022Quote: ... Hydrogels were cultured in 48 well plates for 7 days and maintained in ECM with or without additional growth factors (± 100 ng/mL recombinant human VEGF165; PeproTech 100-20) and hormones ...
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bioRxiv - Neuroscience 2022Quote: ... hNPCs were plated on poly-L-ornithine- and laminin-coated plates and were differentiated with growth media (without FGF-b) supplemented with 25ng/mL recombinant human glial-derived neurotrophic factor (GDNF; PeproTech US, NJ). Fifty percent of cell media were removed every three to four days and replaced with fresh GDNF supplemented media ...
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bioRxiv - Immunology 2022Quote: ... Specificity was validated by performing normalized supernatant ELISAs against the extracellular domain of human TNFR2 (Peprotech, 310-12 and in-house production). Flow cytometry phenotyping of hybridoma cells was performed on a BD FACS Aria III ...
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bioRxiv - Cancer Biology 2024Quote: ... After inhibitor treatment spheroids were treated with a combination of growth factors or vehicle for 30 minutes before fixation in-situ: 100 nM Human IGF1 (Peprotech #100-11) and 100 nM Human EGF (Peprotech #AF-100-15-1mg) ...
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bioRxiv - Neuroscience 2024Quote: ... Acute treatment was evaluated in 9 month-old mice by pre-mixing and co-administering 5 µg HCT-555 with 25 ng recombinant human BDNF (Peprotech, 450-02) into one tibialis anterior muscle and 5 µg HCT-555 with PBS vehicle into the contralateral tibialis anterior ...
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bioRxiv - Immunology 2024Quote: HIOs cultured for 7-12 days were treated with 0.1-10 µM indicated JAK inhibitor 1h prior to 50ng/ml human recombinant IFN-γ (300-02, PeproTech, Rehovot, Israel) stimuli for 24h ...
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bioRxiv - Molecular Biology 2022Quote: ... Cytokine stimulation of human articular chondrocytes was performed on cells at confluence by adding 10ng/ml interleukin-1β (IL-1β, Peprotech, London UK) to standard media and stimulating cells for up to 6 hours.
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bioRxiv - Immunology 2023Quote: All cell lines were seeded in flat 96-well plates at a density of 0.25 × 105 cells/well and cultured for 24 h in the absence or presence of human recombinant IFNγ and TNFα with activities of 2 × 107 Units/mg (PeproTech, Rehovot, Israel). Cells lines were then co-cultured with 100,000 (at density of 1×106 cell/ml ...
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bioRxiv - Cell Biology 2023Quote: ... The medium was changed every day until day 8 when medium was replaced by E6 medium supplemented with 10 ng/mL human Platelet-Derived Growth Factor BB (PDGF-BB, PeproTech, 100-14B) and 2 ng/mL Recombinant Human Transforming Growth Factor-β1 (TGF-β1 ...
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bioRxiv - Immunology 2023Quote: ... 10 million cells were resuspended in 10 ml medium and stimulated with 25ng/ml recombinant human interleukin-2 (IL-2) (Peprotech #AF200-02). Vγ9+Vδ2+ T cells were kept overnight at 37°C and 5% CO2.
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bioRxiv - Bioengineering 2023Quote: ... M1-like MØs were obtained by culturing M0 MØs in RPMI media supplemented with 10pg/ml Lipopolysaccharides (MilliporeSigma, MO, USA) and 1 ng/ml human Interferon-gamma (Peprotech, NJ, USA). Protocol for generating M0 ...
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bioRxiv - Biochemistry 2024Quote: ... Sigma-Aldrich P6407) in OPC proliferation media (DMEM-Sato) supplemented with 10 ng/ml recombinant human ciliary neurotropic factor (CNTF; Peprotech 450-13), 10 µM forskolin (Sigma-Aldrich F6886) ...
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bioRxiv - Cancer Biology 2024Quote: ... Exploration of BMP2 effect on HSJD-DIPG-012 and HSJD-DIPG-014 spheroids was done using 10 ng/mL of human recombinant BMP2 (Peprotech, 120-02C) before protein quantification by western blot.
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Nanoparticle-delivered TLR4 and RIG-I agonists enhance immune response to SARS-CoV-2 subunit vaccinebioRxiv - Immunology 2022Quote: ... RBC-lysed murine bone marrow was seeded at 2 million cells/mL in complete RPMI media with 200 ng/mL human FLT3 ligand (PeproTech, Cat# 300-19) in a 6-well plate (10 million cells/well) ...