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Citations for Peprotech :
1151 - 1200 of 1214 citations for I 309 CCL1 Human since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: ... then 50 µL of 24 h co-culture supernatants or serial dilutions of Recombinant Human IFNγ standard (#300-02, Peprotech, USA) were added to the wells and incubated for 2 h at RT ...
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bioRxiv - Immunology 2023Quote: EndoC-βH1 cells and dispersed human islets were exposed or not to IFNα (2000 U/mL, PeproTech Inc., Rocky Hill, NJ), IFNγ (1000 U/mL ...
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bioRxiv - Immunology 2023Quote: ... Transfected cells were incubated for 24 hours before treatment with 50 ng/mL recombinant human IFN-γ (Peprotech; cat # 300-02) or cDMEM only control for 24 hours ...
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bioRxiv - Bioengineering 2023Quote: ... crosslinked following 10 min exposure to 100 mM CaCl2 and allowed to adsorb for 30 min with recombinant human vascular endothelial growth factor (rhVEGF 165, PeproTech, USA) at 100 μg mL−1 at 4 °C ...
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bioRxiv - Cell Biology 2024Quote: ... 0.5 μM of A83-01 (HY10432, MCE, USA) and 20 ng/mL of recombinant human epidermal growth factor (EGF; AF100-15, PeproTech, USA), 10% KSR and 1% NEAA ...
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bioRxiv - Cancer Biology 2019Quote: ... Samples were examined by ELISA capturing with Fc-iNKG2D and detecting with biotinylated rabbit-anti-human IL-2 polyclonal antibody (Peprotech #500-P22BT) followed by incubation with streptavidin-HRP ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were deprived of serum for 2h and stimulated with 2 ng/ml of recombinant human EGF (Peprotech; Catalog #AF-100-15) for 2 ...
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bioRxiv - Bioengineering 2021Quote: ... Media was serum-free TexMACS (Miltentyi Biotech 170-076-307) supplemented with recombinant human IL2 in concentrations specified by the DOE (Peprotech 200-02). Cell cultures were expanded for 14 days as counted from the time of initial seeding and activation ...
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bioRxiv - Cancer Biology 2019Quote: ... in 200µl of RPMI supplemented with 2% FBS and 5% Matrigel with or without the addition of human IFN-γ (PeproTech; #300-02), mouse IFN-γ (PeproTech ...
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bioRxiv - Bioengineering 2021Quote: ... HepatoSTIM™ medium was supplemented with 10 ng mL−1 of recombinant human TGF-β1 (100-21; PeproTech®, Rocky Hill, NJ), 100 ng mL−1 of recombinant human GDF-15 (120-28C ...
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bioRxiv - Microbiology 2020Quote: ... Frozen activated PBMC cells were thawed and resuspended in complete PBMC media containing RPMI supplemented with 21 ng/mL human IL-2 (Peprotech; #200-02), 10% fetal bovine serum and 1% penicillin-streptomycin ...
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bioRxiv - Microbiology 2020Quote: ... Pellets were resuspended in activation medium containing RPMI supplemented with 21 ng/mL human IL-2 (Peprotech, Rocky Hill, NJ; #200-02), 1.5 μg/mL PHA (Remel ...
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bioRxiv - Cancer Biology 2022Quote: ... the selected monocytes were cultured in complete Advanced-RMPI 1640 supplemented with either 20 ng/mL human GM-CSF (PeproTech, RockyHill, NJ), for M1 differentiation ...
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bioRxiv - Immunology 2022Quote: ... OT-I T cells were activated with 10-8 SIINFEKL or SIITFEKL (Cambridge Peptides) in the presence or absence of recombinant human TGFβ (Peprotech, 5ng/ml). Where indicated ...
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bioRxiv - Immunology 2020Quote: ... cells were washed and cultured for a further 4 days in media containing 20 ng/mL recombinant human IL-2 (Peprotech, London, UK). For analysis of effector cytokine production ...
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bioRxiv - Cancer Biology 2020Quote: ... H1975 cells were cultured in stem cell culture media (Advanced DMEM/F12 [Thermo Fisher Scientific], 1% Glutamax, B-27 supplement [Thermo Fisher Scientific], 10 ng/mL recombinant human HB-EGF [PeproTech, Cranbury ...
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bioRxiv - Pathology 2020Quote: ... HUVEC were plated at a density of 10,000 cells/96-well plate in EGM-2 supplemented with recombinant human TNF-α (300-01A, PeproTech EC Ltd), IL1-β (200-01B ...
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bioRxiv - Developmental Biology 2021Quote: ... LCDMYI supplementation was added as indicated at the following concentrations: 10 ng ml−1 recombinant human LIF (L, 10 ng ml−1; Peprotech, 300-05), CHIR99021 (C ...
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bioRxiv - Immunology 2020Quote: ... Alternately M2 MΦs were activated as follows: M2a MΦs from stimulation with 20 ng/mL recombinant human IL -4 (PeproTech; Rock Hill, NJ, USA) and 25 ng/mL recombinant human IL-13 (PeproTech ...
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bioRxiv - Biochemistry 2022Quote: ... cells were diluted to a density of 6e4/mL and 50 μL (3e3 cells) were added to 50 μL of media containing 2x of a serial dilution of human CSF3 (PeproTech 300-23) in a 96-well plate ...
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bioRxiv - Bioengineering 2022Quote: ... Hydrogels were cultured in 48 well plates for 7 days and maintained in ECM with or without additional growth factors (± 100 ng/mL recombinant human VEGF165; PeproTech 100-20) and hormones ...
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bioRxiv - Neuroscience 2022Quote: ... hNPCs were plated on poly-L-ornithine- and laminin-coated plates and were differentiated with growth media (without FGF-b) supplemented with 25ng/mL recombinant human glial-derived neurotrophic factor (GDNF; PeproTech US, NJ). Fifty percent of cell media were removed every three to four days and replaced with fresh GDNF supplemented media ...
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bioRxiv - Immunology 2022Quote: ... Specificity was validated by performing normalized supernatant ELISAs against the extracellular domain of human TNFR2 (Peprotech, 310-12 and in-house production). Flow cytometry phenotyping of hybridoma cells was performed on a BD FACS Aria III ...
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bioRxiv - Molecular Biology 2022Quote: ... Cytokine stimulation of human articular chondrocytes was performed on cells at confluence by adding 10ng/ml interleukin-1β (IL-1β, Peprotech, London UK) to standard media and stimulating cells for up to 6 hours.
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bioRxiv - Immunology 2023Quote: All cell lines were seeded in flat 96-well plates at a density of 0.25 × 105 cells/well and cultured for 24 h in the absence or presence of human recombinant IFNγ and TNFα with activities of 2 × 107 Units/mg (PeproTech, Rehovot, Israel). Cells lines were then co-cultured with 100,000 (at density of 1×106 cell/ml ...
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bioRxiv - Cancer Biology 2023Quote: ... Exploration of BMP2 effect on HSJD-DIPG-012 and HSJD-DIPG-014 spheroids was done using 10 ng/mL of human recombinant BMP2 (Peprotech, 120-02C) before protein quantification by western blot.
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bioRxiv - Cell Biology 2023Quote: ... The medium was changed every day until day 8 when medium was replaced by E6 medium supplemented with 10 ng/mL human Platelet-Derived Growth Factor BB (PDGF-BB, PeproTech, 100-14B) and 2 ng/mL Recombinant Human Transforming Growth Factor-β1 (TGF-β1 ...
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bioRxiv - Immunology 2023Quote: ... 10 million cells were resuspended in 10 ml medium and stimulated with 25ng/ml recombinant human interleukin-2 (IL-2) (Peprotech #AF200-02). Vγ9+Vδ2+ T cells were kept overnight at 37°C and 5% CO2.
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bioRxiv - Bioengineering 2023Quote: ... M1-like MØs were obtained by culturing M0 MØs in RPMI media supplemented with 10pg/ml Lipopolysaccharides (MilliporeSigma, MO, USA) and 1 ng/ml human Interferon-gamma (Peprotech, NJ, USA). Protocol for generating M0 ...
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bioRxiv - Cancer Biology 2024Quote: ... After inhibitor treatment spheroids were treated with a combination of growth factors or vehicle for 30 minutes before fixation in-situ: 100 nM Human IGF1 (Peprotech #100-11) and 100 nM Human EGF (Peprotech #AF-100-15-1mg) ...
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bioRxiv - Neuroscience 2024Quote: ... Acute treatment was evaluated in 9 month-old mice by pre-mixing and co-administering 5 µg HCT-555 with 25 ng recombinant human BDNF (Peprotech, 450-02) into one tibialis anterior muscle and 5 µg HCT-555 with PBS vehicle into the contralateral tibialis anterior ...
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bioRxiv - Biochemistry 2024Quote: ... Medium was replaced 24 h later with DMEM-10% FCS supplemented or not with 50 ng/ml human IL-1β (PeproTech, 200-01B). Luciferase activity was measured 5 h later using Bright-Glo Luciferase Assay System (Promega ...
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bioRxiv - Immunology 2024Quote: HIOs cultured for 7-12 days were treated with 0.1-10 µM indicated JAK inhibitor 1h prior to 50ng/ml human recombinant IFN-γ (300-02, PeproTech, Rehovot, Israel) stimuli for 24h ...
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Nanoparticle-delivered TLR4 and RIG-I agonists enhance immune response to SARS-CoV-2 subunit vaccinebioRxiv - Immunology 2022Quote: ... RBC-lysed murine bone marrow was seeded at 2 million cells/mL in complete RPMI media with 200 ng/mL human FLT3 ligand (PeproTech, Cat# 300-19) in a 6-well plate (10 million cells/well) ...
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bioRxiv - Immunology 2022Quote: ... The CD34 cell purity was checked by FACS and cells were cultured in IMDM with 10% HI FBS supplemented with human cytokine and growth factor cocktail from Peprotech (hTPO (#300-18) (10 ng/ml) ...
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bioRxiv - Bioengineering 2021Quote: ... supplemented with 50 ng/mL FGF2 and 30 ng/mL animal-free recombinant human Bone Morphogenic Protein 4 (BMP4, Peprotech, AF-120-05ET) for 24 hours ...
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bioRxiv - Cancer Biology 2021Quote: ... The cells were incubated in RPMI1640 for THP-1 culture supplemented with human M-CSF at 20 ng/ml (cross-react with mouse, Peprotech, Cranbury, NJ, USA). After 2 days of culture ...
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bioRxiv - Microbiology 2021Quote: ... control and CSP-stable transfected HepG2 cells were pre-treated with 1 U/mL recombinant human IFN-γ (Peprotech, Rocky Hill, NJ, USA) or equivoluminal medium for 6 h ...
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bioRxiv - Immunology 2020Quote: ... 1640 Medium supplemented with 10% fetal bovine serum (35-076-CV; Coring, New York, USA) and 20 ng/μL Recombinant Human GM-CSF (300-03; Peprotech, Rocky Hill, USA).BEAS-2B cells (CRL-9609 ...
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bioRxiv - Cancer Biology 2021Quote: ... MCF7 cells were cultured in 2D in the absence or presence of 10 ng/mL human TGFβ (PeproTech®, Rocky Hill, NJ, USA) for 3 weeks where TGFβ was replenished every 2 days ...
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bioRxiv - Immunology 2021Quote: ... Human monocyte-derived macrophages (HMDMs) were differentiated from purified CD14+ monocytes in the presence of recombinant human M-CSF (50 ng/ml; PeproTech, Cranbury, NJ, USA) for 6 days in RPMI 1640 medium supplemented with 10% FBS ...
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bioRxiv - Immunology 2020Quote: ... Isolated PBMC were washed with PBS and resuspended by RPMI-1640 which supplemented 50ng/ml recombinant human macrophage colony-stimulating factor (M-CSF) (AF-300-25-500, PeproTech, Cranbury, NJ, USA), then cultured at 37°C in 5% CO2 for 5 days to differentiate into human monocyte derived macrophages (hMDMs ...
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bioRxiv - Immunology 2022Quote: ... Cells were differentiated to macrophages in RPMI x1640 supplemented with 10% FBS containing 10 ng/ml of recombinant human GM-CSF (Peprotech, New Jersey, US) for 7 days ...
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bioRxiv - Bioengineering 2022Quote: ... MDTs were exposed to a neutral culture medium or to a 20 ng/ml of TNF solution (Recombinant TNF alpha human; 300-01A, PeproTech, Thermo Fisher Scientific) in culture medium for either 30 minutes or 240 minutes ...
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bioRxiv - Biochemistry 2023Quote: ... 40,000 cells were seeded per cavity of a 96-well plate and incubated for 20 h with serial dilutions (1000 – 0.076 pM) of the fusion protein Af2-IL-2 and the reference recombinant human IL-2 (PeproTech, Rocky Hill NJ, USA), respectively ...
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bioRxiv - Immunology 2023Quote: ... were differentiated from PBMCs by culturing the cells in RPMI complete medium supplemented with 10% AB-positive human serum and 15 ng/ml M-CSF (PeproTech, 300-25-100UG). After 2 days of culturing ...
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bioRxiv - Developmental Biology 2024Quote: ... proteins were supplemented in Maturation media by dissolving Matrigel at 1% (v/v) containing human recombinant brain-derived neurotrophic factor (BDNF; PeproTech, AF-450-02). The aggregated cells were referred to as cerebral organoids from this stage onward and were allowed to further develop in maturation media until the experimental endpoints ...
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bioRxiv - Immunology 2023Quote: PBMC from healthy donor buffy coats were isolated by Ficoll density centrifugation and rested overnight in 10 ng/mL recombinant human IL-15 (Peprotech, cat #200-15) at 2*106 cells/mL cell culture media (RPMI-1640 ...
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bioRxiv - Immunology 2023Quote: Transplant recipient and healthy donor cryopreserved PBMC were thawed and recovered overnight in 10 ng/mL recombinant human IL-15 (Peprotech, cat #200-15) at 2*106 cells/mL cell culture media (RPMI-1640 ...
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bioRxiv - Immunology 2023Quote: ... iPSCs were differentiated to macrophages[73] and stimulated for 6h and 24h with 10 ng/mL recombinant human interleukin-10 (Peprotech, 200-10-2), 10 ng/mL recombinant human interferon-b (Peprotech ...