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Citations for Peprotech :
1051 - 1100 of 1714 citations for 4 2 Hydroxyimino 2 4 methoxy phenyl ethyl piperazine 1 carboxylic acid tert butyl ester since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: ... After 2 days of culture with 20 ng/ml M-CSF and 20 ng/ml recombinant human RANKL (PeproTech, UK), the wells were rinsed twice with PBS and incubated with 10% bleach solution for 30 min at room temperature ...
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bioRxiv - Immunology 2021Quote: ... Forty-eight hours later cells were lifted off the plates and cultures were supplemented with 100 U/ml recombinant human IL-2 (Peprotech). CFSE labeling for certain experiments was performed prior to seeding on tissue culture plates.
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Neutralizing activity of broadly neutralizing anti-HIV-1 antibodies against primary African isolatesbioRxiv - Immunology 2020Quote: ... A total of 2×106 CD4+ T lymphocytes were activated using anti CD3/CD2/CD28 beads (Miltenyi) and cultured in the presence of 100 U/mL IL-2 (Peprotech) at 37 °C and 5% CO2 ...
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bioRxiv - Immunology 2020Quote: ... Clonal MEL5 CD8+ T cells were maintained in R10 supplemented with 200 IU/ml IL-2 and 25 ng/ml IL-15 (both from PeproTech). The MEL5 TCR is specific for the heteroclitic HLA-A*0201-restricted Melan-A epitope ELAGIGILTV26–35/A27L [17 ...
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bioRxiv - Bioengineering 2022Quote: ... cells were incubated with StemPro-34 medium from days 2 to 5 with a cocktail of growth factors: vascular endothelial growth factor (VEGF; PeproTech), BMP-4 ...
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bioRxiv - Cancer Biology 2022Quote: ... Quiescence was induced by plating cells at a density of 10 cells/mm2 in NSC media in the absence of EGF/FGF-2 and supplemented with BMP4 (10 ng/ml, Peprotech). Cells were treated for 1 day or 3 days ...
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The logic of native enhancer-promoter compatibility and cell-type-specific gene expression variationbioRxiv - Genomics 2022Quote: ... in N2B27 supplemented with 20ng/ml Activin A and 12ng/ml Fibroblast growth factor 2 (FGF2) (MPI-CBG protein facility, or Peprotech) for 14-15 passages ...
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bioRxiv - Neuroscience 2024Quote: ... the medium was changed to NDM supplemented with 20 ng/ml fibroblast growth factor-2 (FGF2; Peprotech, Westlake Village, CA). Media was changed daily ...
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bioRxiv - Microbiology 2024Quote: ... and 1 µg/mL soluble anti-CD28 (clone CD28.2, eBioscience, USA) in the presence of 50 IU/mL IL-2 (PeproTech, USA) for three days ...
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bioRxiv - Immunology 2024Quote: ... Cells were resuspended in complete RMPI (RPMI supplemented with 10% v/v FBS, 100 penicillin, 100 streptomycin) with 50U of IL-2 (PeproTech) and CD3/CD28 Human T-activator Dynabeads (Thermo Fisher ...
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bioRxiv - Immunology 2024Quote: ... cells were thawed and rested in an 37°C / 5% CO2 incubator in the T cell medium with 50 U/mL rhIL-2 (Peprotech, Thermo Fisher Scientific ...
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bioRxiv - Immunology 2024Quote: ... The medium was also supplemented with 10 U/ml of DNaseI and 2 ng/mL of recombinant IL-15 (Peprotech). The cell suspension was transferred to a T25 flask (Corning ...
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bioRxiv - Immunology 2024Quote: ... Approximately 5 × 106 NK cells were co-cultured with 1 × 107 of the modified and irradiated K562 feeder cells in 40 mL of Complete NK MACS medium supplemented with 100 U/mL of recombinant IL-2 (Peprotech) in a T75 flask (Corning) ...
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bioRxiv - Cancer Biology 2024Quote: ... according to manufacturer’s specifications in the presence of 100 IU/mL recombinant human (rh) IL-2 and 10 ng/mL rhIL-7 (Peprotech). Spinoculation with neat 293 Galv9 retroviral supernatant was performed at 48 ...
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bioRxiv - Bioengineering 2024Quote: ... CD8 T cells were transferred to a new 96-well round bottom plate to remove activation and continued to be cultured in complete RPMI supplemented with a higher concentration of IL-2 (10ng/ml; Peprotech).
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bioRxiv - Immunology 2023Quote: ... Isolated T cells were otherwise activated using plate bound anti-CD3 (5 μg/ml, inVIVOMAb Cat# BE0002) in complete medium added with 100 U/ml IL-2 (Peprotech) and soluble αCD28 (0.5 μg/ml ...
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bioRxiv - Immunology 2023Quote: ... T cells were re-plated with fresh medium containing 10 ng/ml rhIL-2 or a combination of 10 ng/ml IL-7 and IL-15 (all Peprotech) and differentiated at a density of 5×105 cells/ml for additional 4 days ...
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bioRxiv - Immunology 2023Quote: ... splenic NK cells were MACs enriched and cultured at 37°C and 5% CO2 in RPMI containing 300U rIL-2 (Peprotech), 50ng/ml rIL-15 (Peprotech ...
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bioRxiv - Bioengineering 2023Quote: ... After gelation samples were submerged in IMR90 medium with/without 2 ng/mL TGF-β2 (100-35B; PeproTech, Cranbury, NJ). Cultures were monitored for loss of collagen adhesion and contraction of the gel for 21 d and survival was quantified as described above.
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bioRxiv - Immunology 2023Quote: ... splenocytes were labelled with cell trace violet (CTV) (ThermoFischer Scientific) and cultured for 48 h with 10 ng/ml rhIL-2 (Peprotech) in vitro ...
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bioRxiv - Immunology 2023Quote: ... Cells were resuspended in complete RMPI (RPMI supplemented with 10% v/v FBS, 100 penicillin, 100 streptomycin) with 50U of IL-2 (PeproTech) and CD3/CD28 Human T-activator Dynabeads (Thermo Fisher ...
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bioRxiv - Cancer Biology 2023Quote: ... HSCL or fetal liver LSK cells were cultured for 2 weeks on OP9 or OP9-DL4 cell layer in HSCL medium supplemented with 10 ng/mL Flt-3 (PeproTech) and 10 ng/mL IL-7 (PeproTech).
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bioRxiv - Immunology 2023Quote: ... mouse bone marrow was flushed and cultured for 2 days in stem cell medium (DMEM + 15% FCS + 25ng/ml SCF (PeproTech) + 10ng/ml IL-3 (PeproTech ...
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bioRxiv - Neuroscience 2023Quote: ... Then NPCs were seeded on Matrigel®-coated flasks at 50 000 cells/cm2 and kept in Astrocyte Induction medium (DMEM/F-12 supplemented with 1x N2 supplement, 1x B27 supplement, 10ng/ml FGF-2 (PeproTech) and 10ng/ml EGF (Miltenyi) ...
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bioRxiv - Immunology 2023Quote: ... cells were washed and then expanded and differentiated in complete IMDM (as above) containing 20 ng/mL recombinant human IL-2 (Peprotech) for a further 4 days ...
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bioRxiv - Immunology 2023Quote: ... The cells were washed and replated in complete RPMI 1640 medium with 100 U/ml IL-2 (PeproTech, 200-02) 48 hours later ...
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bioRxiv - Cell Biology 2024Quote: ... medium was switched to N2B27 medium supplemented with 10 ng/ml PDGF-BB and 2 ng/ml TGFβ-3 (both from PeproTech) for two days to induce differentiation toward pericyte-like cells ...
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bioRxiv - Immunology 2024Quote: ... PBMCs were thawed at 37°C and incubated in complete RPMI media with 10% heat-inactivated FBS and supplemented with IL-2 (50 U/ml; PeproTech). 1 x 104 PBMCs were plated per well in round bottom clear plate (Greiner ...
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bioRxiv - Immunology 2024Quote: ... Samples were stimulated and incubated overnight in complete RPMI media with 10% heat-inactivated FBS and supplemented with IL-2 (50 U/ml; PeproTech).
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bioRxiv - Immunology 2024Quote: ... or the MoFlo XDP cell sorter and rested for 24 hrs in medium containing rIL-2 (10U/ml, cat. 212-12 Peprotech) before activation or adoptive transfer into the animals.
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bioRxiv - Immunology 2024Quote: Single cell suspensions from tumor tissue were isolated as described above and plated in cRPMI containing either 50U/mL IL-2 or 1ng/mL IL-15 (Peprotech) in 12-well culture plates ...
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bioRxiv - Immunology 2024Quote: ... KHYG1 cells were then single-cell sorted into 96-well plates and grown in RPMI-1640 media supplemented with recombinant human IL-2 1000 U/mL (Peprotech) for 5-6 weeks ...
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bioRxiv - Immunology 2024Quote: ... 100 μg/ml streptomycin and 100 units/ml penicillin) and stimulated with plate-bound anti-CD3/CD28 in the presence of 10 ng/ml IL-2 (Peprotech) as indicated ...
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bioRxiv - Neuroscience 2024Quote: ... Cells were then centrifuged at 300g for 5 minutes and resuspended in 2 ml of NDM-A medium supplemented with 20 ng/ml BDNF (Peprotech) and plated onto poly-L-ornithine/laminin-coated dishes at a density of 80,000 cells per cm2 ...
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bioRxiv - Immunology 2024Quote: ... Siglec-Fhi and Siglec-Flow neutrophils were prepared by culturing bone marrow neutrophils for 2 days in the presence or absence of 10 ng/ml GM-CSF (Peprotech) and 10 ng/ml IL-4 (R&D Systems) ...
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bioRxiv - Immunology 2024Quote: ... Every 48 hours the cells were centrifuged and the pellet was resuspended between 1 and 2 × 106 per mL in fresh complete media containing the OVA peptide 3 μg/mL and IL-2 (100 U/mL) after the second stimulation IL-15 (10 ng/mL, Peprotech) was added The number of stimulations is indicated in the figure/text ...
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bioRxiv - Immunology 2024Quote: ... Human peripheral blood mononuclear cells (PBMCs) were preactivated with 30 ng/mL anti-CD3 (OKT3, Miltenyi) and 50 IU/mL IL-2 (PeproTech) and subsequently transduced two times with viral supernatant in the presence of 6 μg/mL polybrene (Sigma ...
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bioRxiv - Immunology 2024Quote: ... NK cells were cultured in U-bottom 96-well plates in cRPMI containing 100 U/ml of IL-2 (Peprotech) for 2 days ...
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bioRxiv - Immunology 2021Quote: ... when the cells were stimulated with 1000 U/ml of rhIL-2 and the combination of 10 ng/ml of recombinant murine (rm) IL-12 (210-12, PeproTech Inc) and 40 ng/ml of rm IL-15 (210-15 ...
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bioRxiv - Developmental Biology 2020Quote: ... DFs cultured in DMEM/F12 with 2% FBS supplemented with TGFβ1 or TGFβ3 (10 ng/ml; PeproTech EC, Ltd., London, UK) or 5 mM citric acid (diluent for lyophilized TGFNβ ...
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bioRxiv - Immunology 2021Quote: ... 1x GlutaMAX™, 50µM 2-mercaptoethanol, 100 units/ml penicillin, and 100µg/ml streptomycin, 10ng/ml mIL-21 PeproTech 210-21). On day8 B cells were harvested and analysed.
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bioRxiv - Bioengineering 2021Quote: ... and re-plated for 2 additional passages at a density of 5 × 103 cells/cm2 at 5% pO2 in expansion medium supplemented with 5 ng/ml of fibroblast growth factor (FGF)-2 (PeproTech Ltd). Medium change was performed three times per week.
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bioRxiv - Cell Biology 2022Quote: ... Recombinant human fibroblast growth factor (FGF-2, catalog no. 100-18B) and transforming growth factor beta (TGF-β, catalog no. 100-21) were purchased from PeproTech, Inc ...
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bioRxiv - Cell Biology 2023Quote: ... at a density of 0.15×106 cell/well and cultured for 2 days in supplemented α-MEM with 30 ng/ml M-CSF (PeproTech, UK). Media were replaced with α-MEM supplemented with 30 ng/ml M-CSF and 30 ng/ml RANKL (PeproTech ...
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bioRxiv - Immunology 2024Quote: ... China) gradient and pulsed with 0.5 µM rUreB protein in the presence of 5 U/ml rmIL-2 (PeproTech, NJ, USA). Cells were cultured in “RF-10” medium consisting of RPMI 1640 (Gibco ...
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bioRxiv - Immunology 2024Quote: ... 50 μM 2-mercaptoethanol) with 100 ng/ml of human FMS-like tyrosine kinase 3 ligand (FLT3L, PeproTech, Cranbury, NJ, USA) for 7 days ...
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bioRxiv - Genomics 2024Quote: ... HFFs treated with IFN were incubated with 550U/ml IFNα (PBL; 11200-2) and 700U/mL IFNβ (Peprotech; 300-02BC-20) and harvested after 5 hours ...
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bioRxiv - Bioengineering 2024Quote: ... They were activated for 24 hours at 37°C and 5% CO2 in Hepes-buffered RPMI medium containing 20 IU/mL recombinant human IL-2 (Peprotech, USA) using anti-CD3/CD28-coated Dynabeads™ (Gibco™ ...
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bioRxiv - Developmental Biology 2020Quote: ... the culture medium was replaced with fresh DMEM/F12 with 2% FBS supplemented with TGFβ1/TGFβ3 (10 ng/ml; PeproTech EC, Ltd., London, UK) or 5mM citric acid (diluent for lyophilized TGFβ ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were deprived of serum for 2h and stimulated with 2 ng/ml of recombinant human EGF (Peprotech; Catalog #AF-100-15) for 2 ...