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Citations for Peprotech :
51 - 100 of 122 citations for Brucella Blood Agar with Hemin and Vitamin K 15x100mm plate 26ml fill since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2022Quote: ... Neural rosettes were enzymatically dissociated and seeded in new POLAM-coated plates with PN medium supplemented with FGF2 (10 ng/ml; Peprotech) and EGF (10 ng/ml ...
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bioRxiv - Neuroscience 2022Quote: ... astrocyte cultures at 80% confluence in 6-well plates (in E6 medium containing BMP4 and FGF2) were treated with vehicle control or IL-1a (5 ng/ml; Peprotech), TNFα (10 ng/ml ...
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bioRxiv - Microbiology 2022Quote: ... 1 × 106 BlaER1 cells per well of a 6-well tissue culture plate were treated with 10 ng/ml human recombinant M-CSF and IL-3 (PeproTech) and 100 nM β-estradiol (Sigma-Aldrich ...
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bioRxiv - Immunology 2022Quote: ... cells were maintained in 24-well G-Rex plates (Wilson Wolf) in NK MACS media (Miltenyi Biotech) supplemented with human IL-2 (100 IU/mL, Peprotech) and human IL-15 (20 ng/mL ...
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bioRxiv - Immunology 2022Quote: ... cells were transferred to 24-well plates and incubated for three days in medium containing 10 U/mL rhIL-2 (Peprotech) to allow establishment of the knockout ...
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bioRxiv - Immunology 2022Quote: ... After a one hour resting period cells were transferred to 24-well plates and incubated for three days in medium containing 10U/mL recombinant human IL-2 (Peprotech) to allow establishment of the knockout ...
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bioRxiv - Immunology 2022Quote: ... The BM cells per animal were resuspended in growth medium and cultured at 1-2 x 106 cells/mL on sterile 6-well plates for 9 days in the presence of 200ng/ml recombinant murine Flt3-Ligand (Peprotech). The BM cells were maintained at 37°C in 5% CO2 ...
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bioRxiv - Immunology 2020Quote: ... and were then cultured at 1-2 x 106/ml in 12-well plates with differentiation media containing 50 ng/ml Interleukin 4 (Peprotech) and 100 ng/ml granulocyte-monocyte colony stimulating factor (GM-CSF ...
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bioRxiv - Immunology 2021Quote: ... Forty-eight hours later cells were lifted off the plates and cultures were supplemented with 100 U/ml recombinant human IL-2 (Peprotech). CFSE labeling for certain experiments was performed prior to seeding on tissue culture plates.
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The skin environment controls local dendritic cell differentiation and function through innate IL-13bioRxiv - Immunology 2021Quote: ... were harvested and one million cells were resuspended in 1 mL of TCM in 24 wells plates and stimulated with recombinant mouse IL-13 (Peprotech) at a concentration of 100 ng/mL for 30 min at 37°C ...
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bioRxiv - Immunology 2020Quote: ... approximately 2 x 106 cells were then seeded per 100 mm plate in 10 ml of media containing 20 ng/ml of recombinant murine (rm) GM-CSF (Peprotech). On day 3 ...
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The logic of native enhancer-promoter compatibility and cell-type-specific gene expression variationbioRxiv - Genomics 2022Quote: ... and seeded to 0.1% gelatinized tissue culture plates in N2B27 supplemented with 10ng/ml FGF2 and 10 ng/ml Epithelial growth factor (EGF) (PeproTech). All cell lines were passaged regularly (every 2 to 4 days ...
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bioRxiv - Biochemistry 2023Quote: ... cells were plated (60,000 cells/well in 24-well plates) two days before treatment in complete media supplemented with 25 ng/mL murine IL-4 (Peprotech) for M2-polarization or without any cytokines (M0) ...
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bioRxiv - Immunology 2023Quote: ... 2×105 BMDMs were seeded into 24-well plates and cultured for 24 h in the presence of 50 ng/mL IFNγ (Peprotech) and 100-1000 U/mL IL2 (Proleukin S ...
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bioRxiv - Microbiology 2023Quote: ... 1 x 106 BLaER1 cells per well of a 6-well tissue culture plate were treated with 10 ng/ml human recombinant M-CSF and IL-3 (PeproTech) and 100 nM β-estradiol (Sigma-Aldrich ...
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bioRxiv - Immunology 2023Quote: ... 4-6 x 106 P14 CD8 T cells were plated in 6 well plates and pre-cultured in IL-7 (10ng/ml, PeproTech) at 37°C ...
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bioRxiv - Neuroscience 2023Quote: ... and plated onto 3 wells of a 6-well plate precoated with GFR Matrigel in N2B27 with 100ng/ml FGFb (100-18B, Peprotech) and 100ng/ml EGF (AF-100-15 ...
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bioRxiv - Immunology 2023Quote: ... The transfection mix was then added dropwise to 2.5×106 M0 macrophages (pre-cultured for 6 days in a 6-well plate in antibiotic-free RPMI1640 macrophage media containing M-CSF (50ng/ml, Peprotech) – with media change on d3) ...
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bioRxiv - Genetics 2023Quote: ... and seeded on a poly-D-lysine/laminin plate with N2B27 media supplemented with BDNF and GDNF (10 ng/ml each; Peprotech). Media were changed the next day to facilitate neuronal maturation and survival ...
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bioRxiv - Immunology 2023Quote: ... were cultured in six-well plates (2 ml per well) in complete RPMI 1640 medium + 10% FBS + 10 ng/ml human IL-4 (PeproTech) + 100 ng/ml human GM-CSF (PeproTech) ...
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bioRxiv - Neuroscience 2023Quote: ... Media was changed every 24h up to day 10 on which cells were mechanically clump passaged using 0.02% EDTA in PBS onto fibronectin coated plates and maintained in the same media without SMAD inhibitors and including 25ng/ml activin-A (Peprotech). All subsequent media changes were half volumes and every 48h before passaging again onto poly-l-ornithine and laminin coated 12w plates on day 20 ...
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bioRxiv - Genetics 2023Quote: ... An aliquot of 500,000 cells were plated in 200 µL of media in a 96-well plate and stimulated with 20 ng/mL of IL-6 (Peprotech) for 15 minutes ...
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bioRxiv - Cell Biology 2023Quote: ... 1x106 cells were plated per well of 96-well round bottom plate and treated with 10ng/mL of TGF-β1 (Peprotech) or equivalent volume of DMSO for 20 minutes at 37°C ...
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bioRxiv - Neuroscience 2024Quote: ... and replated at 50000-75000 cells/cm2 in a poly-L-ornithin-laminin coated 6-well plate with neurobasal medium supplemented with 10 ng/ml PDGFaa (Peprotech), 10 ng/ml IGF1 (Peprotech) ...
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bioRxiv - Cell Biology 2024Quote: ... were routinely cultured on Matrigel-coated plates in hESC culture medium MEF-CM supplemented with 10 ng/ml of bFGF (PeproTech) - with daily medium refreshment ...
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bioRxiv - Cell Biology 2024Quote: ... spheres were dissociated with Accutase and replated on Matrigel-coated plates in ADM supplemented with 10 ng/ml CNTF (PeproTech) and 10 ng/ml BMP-4 for at least 7 days.
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bioRxiv - Immunology 2024Quote: ... 3×105 CD14+ monocytes were seeded in a 12-well plate in 1 mL complete medium containing 20 ng/mL GM-CSF (#300-03; Peprotech) or 50 ng/mL M-CSF (#300-25 ...
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Genetically encoded biosensor for fluorescence lifetime imaging of PTEN dynamics in the intact brainbioRxiv - Neuroscience 2024Quote: ... the TBB solution was removed 1hr later from the plate and replaced with 2ml of Tyrode with or without 100ng/ml EGF (PeproTech) for an additional 2hr ...
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bioRxiv - Immunology 2024Quote: ... KHYG1 cells were then single-cell sorted into 96-well plates and grown in RPMI-1640 media supplemented with recombinant human IL-2 1000 U/mL (Peprotech) for 5-6 weeks ...
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bioRxiv - Immunology 2024Quote: ... 100 μg/ml streptomycin and 100 units/ml penicillin) and stimulated with plate-bound anti-CD3/CD28 in the presence of 10 ng/ml IL-2 (Peprotech) as indicated ...
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bioRxiv - Neuroscience 2024Quote: ... hiPSCs were dissociated to form aggregates in a low-attachment plate and differentiated into mesoderm with CHIR99021 (12 μM, Peprotech) and BMP4 (30 ng/mL ...
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bioRxiv - Immunology 2024Quote: ... NK cells were cultured in U-bottom 96-well plates in cRPMI containing 100 U/ml of IL-2 (Peprotech) for 2 days ...
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bioRxiv - Developmental Biology 2024Quote: ... coated plates in AFX medium which consisted of N2B27 supplemented with 10 ng/mL human/mouse/rat recombinant ACTIVIN A (Peprotech), 12 ng/mL FGF2 (Cambridge Stem Cell Institute ...
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bioRxiv - Immunology 2024Quote: Aliquots of approximately 15,000 pre-cDCs were then cultured for 4 days in a total volume of 150μl in flat-bottomed 96-well plates (Costar, #3596) containing complete RPMI medium supplemented with 20ng/mL GM-CSF (Peprotech) and 400ng/mL Flt3L (Peprotech) ...
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bioRxiv - Immunology 2024Quote: ... Sorted BM Ly6Chi monocytes were cultured for four days in coated plates in the presence of CSF-1 (10ng/ml, Peprotech) at 37°C ...
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bioRxiv - Genomics 2024Quote: ... and seeded to 0.1% gelatinized tissue culture plates in N2B27 supplemented with 10ng/ml FGF2 and 10ng/ml epithelial growth factor (EGF) (PeproTech). NPCs were cultured in 0.1% gelatin-coated plates in N2B27 supplemented with 10ng/ml FGF2 and 10ng/ml EGF.
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bioRxiv - Cancer Biology 2024Quote: ... glioma stem cells were seeded at 5 x 104 cells/well in 24-well plates and cultured in either CD19M or 806M supplemented with of 20 ng/mL epidermal growth factor (EGF) (PeproTech), 20 ng/mL fibroblast growth factor (FGF ...
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bioRxiv - Cell Biology 2022Quote: HUVECs were transfected 48 h before the assay and transferred to gelatin-coated black-walled 96-well plates (Falcon) and stimulated with TNF-α (10 ng/ml; Peprotech) or PBS (Gibco ...
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bioRxiv - Immunology 2020Quote: PBMCs were plated in 24-well plates at 2×106 cells per well with 100 IU/ml IL-2 (PeproTech, USA) in the complete medium ...
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bioRxiv - Cancer Biology 2022Quote: Wild-type mouse colonic organoids were plated onto 24-well plates in Matrigel and allowed to culture for 5 days before treatment with recombinant murine INF-y (PeproTech, UK) at concentrations of 0.2 ...
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bioRxiv - Cancer Biology 2022Quote: ... were plated onto 24-well plates in Matrigel and cultured for 3 days before treatment with either recombinant murine INF-y (PeproTech, UK) at 1 ng/mL or recombinant murine TGFβ 1 (R&D ...
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bioRxiv - Cancer Biology 2022Quote: ... PBMCs were harvested from K3EDTA anti-coagulated whole blood by density gradient centrifugation with Lymphoprep and plated in 12 well plates at 2-4 × 106 cells per well in 10% RPMI + 10 ng/mL human CSF (300-25, Peprotech, UK). Medium was replaced every 2-3 days until adherent populations of macrophages had established ...
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bioRxiv - Immunology 2024Quote: ... Transduced cells were incubated for 4 days with plate-bound anti-CD3/anti-CD28 in media supplemented with hIL-2 (0.0344 units/mL, Peprotech #200-02). Transduction efficiency was assessed for each experiment by flow cytometry.
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bioRxiv - Cancer Biology 2024Quote: ... and plated into 96-well ultra-low attachment V-bottomed plates (PHCBI) in mTEsR plus basal media (StemCell) supplemented with 10uM Y-27632 Dihydrochloride (PeproTech, #1293823) at 6,000 cells per well ...
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bioRxiv - Bioengineering 2021Quote: ... The specimens with the attached PBMCs were subsequently placed into 24 well plates and then differentiated by the addition of 20 ng/mL RANKL (Peprotech 310-01) and 10 ng/mL M-CSF (R&D ...
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bioRxiv - Cell Biology 2022Quote: ... cells were lifted using accutase and plated onto Laminin-111/ poly-L-ornithine-coated plates in NIM supplemented with 10 ng/ml BDNF (Peprotech, 450-02) and 10 ng/ml GDNF (Peprotech ...
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bioRxiv - Neuroscience 2020Quote: For the ADV transduction experiments human iPSC derived NSCs were plated at 700,000 cells per well of a 6-well plate in 2 ml NPM supplemented with 25 ng/ml βFGF (Peprotech, 100-18B) and 25 ng/ml Activin A (Peprotech ...
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bioRxiv - Pathology 2020Quote: ... HUVEC were plated at a density of 10,000 cells/96-well plate in EGM-2 supplemented with recombinant human TNF-α (300-01A, PeproTech EC Ltd), IL1-β (200-01B ...
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bioRxiv - Cell Biology 2022Quote: ... After 24 hours cells were re-plated at 30,000 cells/ml in gelatinized 24-well plates in DMEM complete medium supplemented with IL-3 (Peprotech, Cat#213-13) mCSF-1 (Peprotech ...
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bioRxiv - Bioengineering 2024Quote: ... Cells were then moved to a flat-bottom 96 well plate containing 160 uL of medium supplemented with 500 U/mL IL-2 (Peprotech, Cranbury, NJ) or 10 ng/mL of IL-7 (BioTechne ...