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Citations for Peprotech :
851 - 900 of 1574 citations for Mouse Lymphoid Enhancer Binding Factor 1 LEF1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2021Quote: All growth factors and drugs were prepared and reconstituted according to the instructions of the manufacturers: EGF (PeproTech, AF-100-15) 50 ng ml-1 in PBS with 1% w/v BSA ...
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bioRxiv - Cell Biology 2023Quote: ... On day 3, medium was replaced with E6 supplemented with 50 ng/mL vascular endothelial growth factor (VEGF, (PeproTech, 100-20), 10 ng/mL FGF2 and 20 ng/mL rBMP4 ...
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bioRxiv - Bioengineering 2023Quote: ... crosslinked following 10 min exposure to 100 mM CaCl2 and allowed to adsorb for 30 min with recombinant human vascular endothelial growth factor (rhVEGF 165, PeproTech, USA) at 100 μg mL−1 at 4 °C ...
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bioRxiv - Cell Biology 2024Quote: ... 0.5 μM of A83-01 (HY10432, MCE, USA) and 20 ng/mL of recombinant human epidermal growth factor (EGF; AF100-15, PeproTech, USA), 10% KSR and 1% NEAA ...
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bioRxiv - Immunology 2022Quote: ... mouse IL-2 (Peprotech), and human TGF-beta (Peprotech) ...
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bioRxiv - Microbiology 2019Quote: ... and 10 healthy controls (sampled once each) were quantified by two-step sandwich ELISA following the manufacturer’s instructions (Peprotech Human BD-2 and BD-3 ...
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bioRxiv - Synthetic Biology 2020Quote: ... cells were spun down and supernatants were collected and analyzed by ELISA for IL-7 following the manufacturer’s recommended protocol (Peprotech).
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bioRxiv - Immunology 2019Quote: ... into B cell culture medium supplemented with 1 ng/mL recombinant mouse IL-4 (PeproTech Ca.# 214-14) and 2 μg/mL anti-mouse RP105 clone RP/14.
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bioRxiv - Cell Biology 2019Quote: ... medium is made with DMEM/F12 together with the following components: 100 ng/mL Fetal Growth Factor-2 (FGF2 Peprotech #450-33), 100 ng/mL Epidermal Growth factor (EGF ...
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bioRxiv - Cell Biology 2020Quote: ... cells were plated in 10 cm diameter Petri dishes with 10 ng/mL macrophage colony-stimulating factor (M-CSF, PeproTech, London, UK) for 24 h ...
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bioRxiv - Cancer Biology 2020Quote: ... 6 ng/ml murine Interleukin 3 (mIL3) and 20 ng/ml murine stem cell factor (mSCF) (all from Peprotech, Rocky Hill, NJ), and incubated in 5% CO2 at 37°C ...
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bioRxiv - Cancer Biology 2021Quote: ... 10% FBS) in the presence of macrophage colony stimulating factor (M-CSF) (30 ng/mL, #315-02, Peprotech, Rocky Hill, NJ, USA). After three days in culture ...
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bioRxiv - Immunology 2019Quote: ... 50μM 2-ME and 10 ng/ml of recombinant murine granulocyte– macrophage colonystimulating factor (GM-CSF, PeproTech Inc., Rocky Hill, NJ, USA) for 7 days ...
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bioRxiv - Bioengineering 2022Quote: ... Hydrogels were cultured in 48 well plates for 7 days and maintained in ECM with or without additional growth factors (± 100 ng/mL recombinant human VEGF165; PeproTech 100-20) and hormones ...
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bioRxiv - Neuroscience 2022Quote: ... hNPCs were plated on poly-L-ornithine- and laminin-coated plates and were differentiated with growth media (without FGF-b) supplemented with 25ng/mL recombinant human glial-derived neurotrophic factor (GDNF; PeproTech US, NJ). Fifty percent of cell media were removed every three to four days and replaced with fresh GDNF supplemented media ...
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bioRxiv - Immunology 2023Quote: ... 55 uM 2-mercaptoethanol and 20 ng/ml of recombinant murine granulocyte-macrophage colony-stimulating factor (PeproTech, Rocky Hill, NJ, United States)] at 1:5 apoptotic cancer cell to cDC1 ratio ...
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bioRxiv - Microbiology 2023Quote: ... and 50 μM β-mercaptoethanol and differentiated with 20 ng/ml murine granulocyte-macrophage colony-stimulating factor (GM-CSF) (Peprotech, 315-03). BMDCs were stained for CD11c and analyzed by flow cytometry (LSR-Fortessa flow cytometer ...
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bioRxiv - Cancer Biology 2023Quote: ... 6 ng/ml murine interleukin 3 (mIL3) and 20 ng/ml murine stem cell factor (mSCF) (all from Peprotech, Rocky Hill, NJ), and incubated at 5% CO2 and 37°C ...
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bioRxiv - Cell Biology 2023Quote: ... The medium was changed every day until day 8 when medium was replaced by E6 medium supplemented with 10 ng/mL human Platelet-Derived Growth Factor BB (PDGF-BB, PeproTech, 100-14B) and 2 ng/mL Recombinant Human Transforming Growth Factor-β1 (TGF-β1 ...
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bioRxiv - Immunology 2023Quote: ... in the presence of 40 ng/ml of granulocyte-macrophage colony-stimulating factor (GM-CSF) and IL-4 (both from Peprotech or Biolegend). Cells were cultured in 24-well plates in DMEM (Gibco) ...
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bioRxiv - Cancer Biology 2023Quote: ... unsorted bone marrow cells from Myc+/+ or MycT58A/T58Amice (25,000 cells/ml) were cultured in IMDM containing stem cell factor (SCF Peprotech, 50 ng/ml), IL-6 (Peprotech ...
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bioRxiv - Cancer Biology 2024Quote: ... After inhibitor treatment spheroids were treated with a combination of growth factors or vehicle for 30 minutes before fixation in-situ: 100 nM Human IGF1 (Peprotech #100-11) and 100 nM Human EGF (Peprotech #AF-100-15-1mg) ...
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bioRxiv - Immunology 2024Quote: ... cells were isolated from bone marrow of C57Bl/6 mice and cultured with granulocyte-macrophage stimulating factor (100ng or 2×103 units per 3×106 cells, Peprotech, Cranbury, NJ) in RPMI-1640 media containing 10% heat inactivated fetal bovine serum ...
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bioRxiv - Cell Biology 2023Quote: ... BMMs were seeded at a density of 1.2 × 105 cells/well into 24-well culture plates and incubated in α-MEM (SH30265.01; Hyclone, Rockford, IL, USA) containing 20 ng/mL macrophage colony-stimulating factor (M-CSF) (300-25; PeproTech, Cranbury, NJ, USA). To induce osteoclast differentiation ...
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bioRxiv - Cell Biology 2020Quote: ... Quantification of released BMP-2 was accomplished using a α-BMP-2 enzyme-linked immunosorbent (ELISA) assay (Peprotech, Hamburg, Germany) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2019Quote: IL-2 complex (IL-2C) was prepared as described(79) by incubating 1 μg recombinant mouse IL-2 (PeproTech) with 5 μg purified anti-mouse IL-2 antibody (JES6-1 ...
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bioRxiv - Immunology 2023Quote: ... The following anti-mouse primary antibodies were used: anti-RELM-beta (1:500, rabbit polyclonal antibody, Peprotech, 500-p215), anti-MUC2 (1:200 ...
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bioRxiv - Immunology 2021Quote: ... Mouse recombinant IL-21 (PeproTech) was added to co-cultures at 10ng/ml ...
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bioRxiv - Immunology 2021Quote: ... recombinant mouse IL-12 (Peprotech) and anti-IL-4 (BioXCell) ...
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bioRxiv - Cell Biology 2021Quote: ... 10ng/mL mouse SCF (Peprotech) and 0.1% PVA (Sigma ...
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bioRxiv - Cancer Biology 2022Quote: ... mouse SCF (20ng/µL, PeproTech), penicillin (100U/mL ...
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bioRxiv - Cancer Biology 2022Quote: ... mouse IFN-γ from PeproTech; MRT68601 ...
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bioRxiv - Biophysics 2021Quote: ... EMT in all cells lines were induced by addition of 5ng/ml Targeted Growth Factor-β1 (TGFβ1) (Peprotech, catalog no. 100-21-10UG) for 48 hours62.
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bioRxiv - Immunology 2022Quote: ... The CD34 cell purity was checked by FACS and cells were cultured in IMDM with 10% HI FBS supplemented with human cytokine and growth factor cocktail from Peprotech (hTPO (#300-18) (10 ng/ml) ...
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bioRxiv - Cell Biology 2021Quote: The immature motor neurons were seeded on the cell imaging coverglasses in a low density (1.5×104 cells/cm2) and grown for 2 days in the fourth step medium supplemented with neurotrophic factor (NTF) cocktail: IGF1 (PeproTech, 10 ng/ml), CNTF (PeproTech ...
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bioRxiv - Cell Biology 2021Quote: ... cells were treated for 4 days with 50 ng/mL of brain-derived growth factor (BDNF) (Peprotech, Rocky Hill, NJ, USA, #450-02B) in serum-depleted EMEM/F12 media supplemented with 1% pen/strep 88,89.
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bioRxiv - Immunology 2020Quote: ... Isolated PBMC were washed with PBS and resuspended by RPMI-1640 which supplemented 50ng/ml recombinant human macrophage colony-stimulating factor (M-CSF) (AF-300-25-500, PeproTech, Cranbury, NJ, USA), then cultured at 37°C in 5% CO2 for 5 days to differentiate into human monocyte derived macrophages (hMDMs ...
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bioRxiv - Neuroscience 2022Quote: ... 10k primary rat microglia were added to 20k DIV12 primary hippocampal neurons with 40 ng/mL of rat macrophage colony stimulating factor (MCSF) (Peprotech 400-28-100UG). At the time of plating ...
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bioRxiv - Microbiology 2023Quote: ... 100 ⍰g/ml streptomycin and 50 ⍰M β-mercaptoethanol plus 20 ng/ml murine granulocyte-macrophage colony-stimulating factor (GM-CSF) (Peprotech, 315-03). On day 4 ...
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bioRxiv - Cell Biology 2023Quote: ... neuromuscular co-cultured were gently covered with 2 mL of DM containing brain-derived neurotrophic factor (BDNF, 10 ng/mL, PeproTech 450-02-10μg) and glial cell-derived neurotrophic factor (GDNF ...
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bioRxiv - Bioengineering 2022Quote: ... The supernatant was discarded and the cells were re-suspended in BMDM (the same supplemented DMEM used for nerve fibroblasts, further supplemented with macrophage colony stimulating factor [400-28, Peprotech; 50 ng/ml]). Cells were counted in a hemocytometer and seeded on 100 x 15 mm uncoated petri dishes (Sigma ...
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bioRxiv - Bioengineering 2023Quote: ... NPSCs were expanded using NPSC proliferation media (NPM) composed of N2B27 supplemented with 20 ng/ml epidermal growth factor (EGF; Peprotech, AF-100-15), 20ng/ml basic fibroblast growth factor (bFGF ...
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bioRxiv - Genetics 2023Quote: ... EB-derived neural rosettes were dissociated into single cells with Accutase for 5⍰min at 37°C and plated on Matrigel or polyornithine/laminin-coated plates in the NIM complete medium supplemented with FGF2 (101ng/mL) and epidermal growth factor (EGF) (10⍰ng/mL; PeproTech, Rocky Hill, NJ). Cells were expanded for several passages as a homogeneous population of NPCs.
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bioRxiv - Neuroscience 2019Quote: ... The concentration of TNF in cell culture supernatants was quantified by enzyme-linked immunosorbent assay (ELISA) following the instructions provided by the manufacturer (Peprotech, UK). Absorbance at 405 nm ...
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bioRxiv - Neuroscience 2020Quote: ... The final pellets were dissociated with pre-warmed 10% fetal bovine serum in DMEM supplemented with 20 ng mL-1 mouse M-CSF (PeproTech) and plated in culture dishes ...
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The skin environment controls local dendritic cell differentiation and function through innate IL-13bioRxiv - Immunology 2021Quote: ... were harvested and one million cells were resuspended in 1 mL of TCM in 24 wells plates and stimulated with recombinant mouse IL-13 (Peprotech) at a concentration of 100 ng/mL for 30 min at 37°C ...
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bioRxiv - Bioengineering 2021Quote: PTMs were differentiated from hiPSCs using the above protocol for hiPSC-derived Leydig cells with the modification that PDGF-AA is replaced by human recombinant Platelet-Derived Growth Factor-BB (PDGF-BB, Peprotech, 100-14B, Figure 1B). On day 25 the cells were expanded on PLL-coated plates in DMEM/F12 ...
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bioRxiv - Neuroscience 2020Quote: ... and 1% insulin transferrin selenium (Cat N° 41400045) in IMDM (Cat N° 12440053) supplemented with 5 ng/ml recombinant human basic fibroblast growth factor (bFGF) (Peprotech, Cat N° 100-18B). Medium was changed every 4 days ...
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bioRxiv - Immunology 2022Quote: ... hCD34+ cells were either kept in culture at 37°C in RPMI medium with human recombinant Stem Cell Factor (SCF) at 50 ng/ml (PeproTech, Rocky Hill, NJ, USA), human recombinant thrombopoietin (TPO ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Differentiated sensory neurons were maintained in N2 medium with neuronal growth factors (10 ng/ml human β-NGF, BDNF, NT3 and GDNF; PeproTech, Rocky Hill, NJ) in a 37°C incubator under 5% CO2 ...