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Citations for Peprotech :
851 - 900 of 932 citations for Complement Factor B CFB Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: ... 6 ng/ml murine interleukin 3 (mIL3) and 20 ng/ml murine stem cell factor (mSCF) (all from Peprotech, Rocky Hill, NJ), and incubated at 5% CO2 and 37°C ...
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bioRxiv - Bioengineering 2023Quote: ... OPCs were cultured using OPC maintenance/proliferation media (OMM) composed of N2B27 supplemented with 10ng/ml insulin-like growth factor (IGF-1; Peprotech, 100-11), 15ng/ml platelet-derived growth factor AA (PDGFaa ...
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bioRxiv - Cell Biology 2023Quote: ... The medium was changed every day until day 8 when medium was replaced by E6 medium supplemented with 10 ng/mL human Platelet-Derived Growth Factor BB (PDGF-BB, PeproTech, 100-14B) and 2 ng/mL Recombinant Human Transforming Growth Factor-β1 (TGF-β1 ...
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bioRxiv - Immunology 2023Quote: ... in the presence of 40 ng/ml of granulocyte-macrophage colony-stimulating factor (GM-CSF) and IL-4 (both from Peprotech or Biolegend). Cells were cultured in 24-well plates in DMEM (Gibco) ...
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bioRxiv - Cancer Biology 2023Quote: ... unsorted bone marrow cells from Myc+/+ or MycT58A/T58Amice (25,000 cells/ml) were cultured in IMDM containing stem cell factor (SCF Peprotech, 50 ng/ml), IL-6 (Peprotech ...
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bioRxiv - Immunology 2024Quote: ... cells were isolated from bone marrow of C57Bl/6 mice and cultured with granulocyte-macrophage stimulating factor (100ng or 2×103 units per 3×106 cells, Peprotech, Cranbury, NJ) in RPMI-1640 media containing 10% heat inactivated fetal bovine serum ...
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bioRxiv - Cancer Biology 2024Quote: ... After inhibitor treatment spheroids were treated with a combination of growth factors or vehicle for 30 minutes before fixation in-situ: 100 nM Human IGF1 (Peprotech #100-11) and 100 nM Human EGF (Peprotech #AF-100-15-1mg) ...
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bioRxiv - Cell Biology 2023Quote: ... BMMs were seeded at a density of 1.2 × 105 cells/well into 24-well culture plates and incubated in α-MEM (SH30265.01; Hyclone, Rockford, IL, USA) containing 20 ng/mL macrophage colony-stimulating factor (M-CSF) (300-25; PeproTech, Cranbury, NJ, USA). To induce osteoclast differentiation ...
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bioRxiv - Immunology 2019Quote: ... into B cell culture medium supplemented with 1 ng/mL recombinant mouse IL-4 (PeproTech Ca.# 214-14) and 2 μg/mL anti-mouse RP105 clone RP/14.
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bioRxiv - Biophysics 2021Quote: ... EMT in all cells lines were induced by addition of 5ng/ml Targeted Growth Factor-β1 (TGFβ1) (Peprotech, catalog no. 100-21-10UG) for 48 hours62.
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bioRxiv - Immunology 2022Quote: ... The CD34 cell purity was checked by FACS and cells were cultured in IMDM with 10% HI FBS supplemented with human cytokine and growth factor cocktail from Peprotech (hTPO (#300-18) (10 ng/ml) ...
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bioRxiv - Cell Biology 2021Quote: The immature motor neurons were seeded on the cell imaging coverglasses in a low density (1.5×104 cells/cm2) and grown for 2 days in the fourth step medium supplemented with neurotrophic factor (NTF) cocktail: IGF1 (PeproTech, 10 ng/ml), CNTF (PeproTech ...
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bioRxiv - Cell Biology 2021Quote: ... cells were treated for 4 days with 50 ng/mL of brain-derived growth factor (BDNF) (Peprotech, Rocky Hill, NJ, USA, #450-02B) in serum-depleted EMEM/F12 media supplemented with 1% pen/strep 88,89.
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bioRxiv - Immunology 2020Quote: ... Isolated PBMC were washed with PBS and resuspended by RPMI-1640 which supplemented 50ng/ml recombinant human macrophage colony-stimulating factor (M-CSF) (AF-300-25-500, PeproTech, Cranbury, NJ, USA), then cultured at 37°C in 5% CO2 for 5 days to differentiate into human monocyte derived macrophages (hMDMs ...
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bioRxiv - Neuroscience 2022Quote: ... 10k primary rat microglia were added to 20k DIV12 primary hippocampal neurons with 40 ng/mL of rat macrophage colony stimulating factor (MCSF) (Peprotech 400-28-100UG). At the time of plating ...
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bioRxiv - Cell Biology 2023Quote: ... neuromuscular co-cultured were gently covered with 2 mL of DM containing brain-derived neurotrophic factor (BDNF, 10 ng/mL, PeproTech 450-02-10μg) and glial cell-derived neurotrophic factor (GDNF ...
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bioRxiv - Bioengineering 2022Quote: ... The supernatant was discarded and the cells were re-suspended in BMDM (the same supplemented DMEM used for nerve fibroblasts, further supplemented with macrophage colony stimulating factor [400-28, Peprotech; 50 ng/ml]). Cells were counted in a hemocytometer and seeded on 100 x 15 mm uncoated petri dishes (Sigma ...
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bioRxiv - Microbiology 2023Quote: ... 100 ⍰g/ml streptomycin and 50 ⍰M β-mercaptoethanol plus 20 ng/ml murine granulocyte-macrophage colony-stimulating factor (GM-CSF) (Peprotech, 315-03). On day 4 ...
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bioRxiv - Bioengineering 2023Quote: ... NPSCs were expanded using NPSC proliferation media (NPM) composed of N2B27 supplemented with 20 ng/ml epidermal growth factor (EGF; Peprotech, AF-100-15), 20ng/ml basic fibroblast growth factor (bFGF ...
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bioRxiv - Genetics 2023Quote: ... EB-derived neural rosettes were dissociated into single cells with Accutase for 5⍰min at 37°C and plated on Matrigel or polyornithine/laminin-coated plates in the NIM complete medium supplemented with FGF2 (101ng/mL) and epidermal growth factor (EGF) (10⍰ng/mL; PeproTech, Rocky Hill, NJ). Cells were expanded for several passages as a homogeneous population of NPCs.
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bioRxiv - Immunology 2024Quote: ... and cultured in DC media (15% FBS, 1% A/A, 50µM α-MTG, 20ng/mL granulocyte-macrophage colony stimulating factor (GM-CSF; Peprotech cat#: 315-03)
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bioRxiv - Developmental Biology 2024Quote: ... proteins were supplemented in Maturation media by dissolving Matrigel at 1% (v/v) containing human recombinant brain-derived neurotrophic factor (BDNF; PeproTech, AF-450-02). The aggregated cells were referred to as cerebral organoids from this stage onward and were allowed to further develop in maturation media until the experimental endpoints ...
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bioRxiv - Developmental Biology 2021Quote: ... NMP differentiation medium (-SBSAG/-SBLDN) or NMP differentiation medium supplemented with 50 ng/ml GDF11 (Peprotech, 120-11-B). Samples were collected for RNA analysis when confluent (48-72h).
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bioRxiv - Immunology 2020Quote: ... Primary splenic B cell stimulation was performed with αCD40 (1 μg/ml, eBioscience) plus IL4 (20 ng/ml, PeproTech) or with LPS (25 ng/ml ...
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bioRxiv - Bioengineering 2021Quote: PTMs were differentiated from hiPSCs using the above protocol for hiPSC-derived Leydig cells with the modification that PDGF-AA is replaced by human recombinant Platelet-Derived Growth Factor-BB (PDGF-BB, Peprotech, 100-14B, Figure 1B). On day 25 the cells were expanded on PLL-coated plates in DMEM/F12 ...
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bioRxiv - Neuroscience 2020Quote: ... and 1% insulin transferrin selenium (Cat N° 41400045) in IMDM (Cat N° 12440053) supplemented with 5 ng/ml recombinant human basic fibroblast growth factor (bFGF) (Peprotech, Cat N° 100-18B). Medium was changed every 4 days ...
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bioRxiv - Immunology 2022Quote: ... hCD34+ cells were either kept in culture at 37°C in RPMI medium with human recombinant Stem Cell Factor (SCF) at 50 ng/ml (PeproTech, Rocky Hill, NJ, USA), human recombinant thrombopoietin (TPO ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Differentiated sensory neurons were maintained in N2 medium with neuronal growth factors (10 ng/ml human β-NGF, BDNF, NT3 and GDNF; PeproTech, Rocky Hill, NJ) in a 37°C incubator under 5% CO2 ...
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bioRxiv - Neuroscience 2023Quote: ... was added at a final concentration of 3.4 mg/mL to prevent the growth of non-neuronal cells and supplemented with 50 ng/mL murine β-nerve growth factor (PeproTech, Cat# 450-34) with 3 mg/mL DNAse I grade II (Roche ...
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bioRxiv - Neuroscience 2022Quote: ... and plated on low-attachment 6-well plate in E8 with 20 ng/ml human basic fibroblast growth factor (bFGF2; Peprotech, Somerset County, NJ, USA) and 20 µM Rho kinase inhibitor (Sigma-Aldrich ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Immature iPSC-SNs were maintained in N2 medium supplemented with human growth factors (25 ng/mL NGF-β, 450-01; BDNF, 450-02; GDNF, 450-10; NT-3, 450-03; Peprotech, Cranbury, NJ, USA), and 0.2 mM L-ascorbic acid (A4403 ...
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bioRxiv - Genetics 2023Quote: ... and transferred to low adherence 10 cm plates in N2B27 medium supplemented with 10 ng/mL murine epidermal growth factor (Peprotech Inc. 315-09-100UG) and 10 ng/mL fibroblast growth factor 2 (Cedarlane Labs CLCYT386) ...
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bioRxiv - Cancer Biology 2023Quote: ... 10 ng/mL IL-3 (Cat#200-03), and 10 ng/mL granulocyte colony-stimulating factor (G-CSF, Cat#300-23) (all from Peprotech, Rock Hill, NJ, USA). Additionally ...
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bioRxiv - Immunology 2022Quote: ... CD14+ cells were incubated for 6 days in the presence of 50 ng/ml granulocyte-macrophage colony- stimulating factor (GM-CSF) and 20 ng/ml interleukin-4 (IL-4) (PeproTech®, Neuilly sur Seine, France). The medium used for culture was RPMI ...
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bioRxiv - Developmental Biology 2021Quote: ... the SC solution was centrifuged at 300 g for 5 minutes to remove the supernatant and SFM containing 50 μg/ml stem cell factor (PeproTech, 300-07, Cranbury, NJ, USA), 100 μg/ml IL-3 (PeproTech ...
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bioRxiv - Immunology 2022Quote: ... 0.25 µg/mL Amphotericin B and 2 mM L-glutamine and treated with 10 ng/mL recombinant IL-12p70 (Peprotech) for 24 hours for supernatant IFN-γ analysis.
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bioRxiv - Neuroscience 2020Quote: ... In all experiments cells were cultivated for 72 h in culture medium as described above with addition of 10ng/mL human b-FGF (Peprotech) and culture medium was changed everyday ...
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bioRxiv - Bioengineering 2023Quote: ... After 2 days the coculture medium was replaced by coculture medium additionally containing 50 ng/mL receptor activator of NFκ-B ligand (RANKL, 310-01, PeproTech). Medium was changed weekly inside the incubator via the self-healing injection ports by removing 3 mL of the total 6 mL and replacing it by 3 mL fresh coculture medium with double the concentration of dexamethasone ...
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bioRxiv - Biochemistry 2022Quote: ... For dot blot analysis of BMP-7 in conditioned media from co-culture assays 5 µl were applied to a nitrocellulose membrane and BMP-7 was detected using a rabbit pAb anti-BMP-7 GF at 1:2000 (#500-P198 B, Peprotech). For dot blot analysis in conditioned media of 3D organotypic co-cultures ...
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bioRxiv - Cell Biology 2024Quote: ... and cultivated in 4 mL of B-cell medium containing either 10 ng·mL−1 rIL-4 or 10 ng·mL−1 rIL-21 (Peprotech, 210-21). On day 8 ...
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bioRxiv - Neuroscience 2021Quote: ... Cells were dissociated using 5mM EDTA in HBSS and resuspended in media with B-27 supplement for 4 hours before incubation with recombinant BDNF protein (100nM, Peprotech, #450-02) or vehicle (equal volume of 0.1% BSA in H2O) ...
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bioRxiv - Immunology 2022Quote: ... Cells were transdifferentiation for 7 days at 60,000 cells/cm2 in medium containing 50 ng/ml IL-3 (200-03 B, Peprotech, New Jersey, USA), 50 ng/ml M-CSF (300-25 B ...
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bioRxiv - Systems Biology 2020Quote: ... on Day 1 we changed the medium to RPMI + 2% B-27 (minus insulin) + 5ng/ml BMP4 (Peprotech, cat. AF-120-05ET) + 1uM Chiron 99021 and incubated these cells for 48 hours ...
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bioRxiv - Cancer Biology 2020Quote: ... H1975 cells were cultured in stem cell culture media (Advanced DMEM/F12 [Thermo Fisher Scientific], 1% Glutamax, B-27 supplement [Thermo Fisher Scientific], 10 ng/mL recombinant human HB-EGF [PeproTech, Cranbury ...
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bioRxiv - Immunology 2020Quote: ... Cells were treated with Mouse active perforin (#APB317Mu01, Cloud-Clone Corp., Katy, TX) and recombinant mouse Granzyme B (#140-03, PeproTech, Rocky Hill, NJ) for 4 to 6 hours in indi cated concentrations following manufacturer’s recommendations.
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bioRxiv - Neuroscience 2020Quote: ... Goat anti-mCSF1 antibody (PeproTech) was applied and incubated at room temperature overnight ...
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bioRxiv - Immunology 2020Quote: ... All primary anti-chemokine antibodies used in this study were purified polyclonal rabbit antibodies from Peprotech. Recombinant human MFG-E8 protein produced in mouse myeloma cells was purchased from R&D Systems (Minneapolis ...
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bioRxiv - Neuroscience 2021Quote: ... Primary antibody for CXCL10 (Peprotech 1:10,000) was incubated at 4 degrees overnight in 10% normal goat serum ...
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bioRxiv - Genetics 2022Quote: ... clone 145-2C11, WEHI antibody facility), anti-CD28 (2μg/ml, clone 3751, WEHI antibody facility) and human IL-2 (Peprotech). CD8 T cell cultures contained 25μg/ml anti-mouse IL2 (clone S4B6 ...
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bioRxiv - Immunology 2023Quote: ... The following anti-mouse primary antibodies were used: anti-RELM-beta (1:500, rabbit polyclonal antibody, Peprotech, 500-p215), anti-MUC2 (1:200 ...