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Citations for Peprotech :
851 - 900 of 913 citations for 6 Bromo 2 pyridineacetic acid methyl ester since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... 1x GlutaMAX™, 50µM 2-mercaptoethanol, 100 units/ml penicillin, and 100µg/ml streptomycin, 10ng/ml mIL-21 PeproTech 210-21). On day8 B cells were harvested and analysed.
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bioRxiv - Immunology 2019Quote: ... and maintained in HIPP™-T009 medium (Bioengine) in the presence of 2% autoserum and IL-21 (30 ng/ml, PeproTech) in a 37 °C 5% CO2 incubator for 12 days ...
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bioRxiv - Bioengineering 2021Quote: ... and re-plated for 2 additional passages at a density of 5 × 103 cells/cm2 at 5% pO2 in expansion medium supplemented with 5 ng/ml of fibroblast growth factor (FGF)-2 (PeproTech Ltd). Medium change was performed three times per week.
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bioRxiv - Cell Biology 2022Quote: ... Recombinant human fibroblast growth factor (FGF-2, catalog no. 100-18B) and transforming growth factor beta (TGF-β, catalog no. 100-21) were purchased from PeproTech, Inc ...
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bioRxiv - Developmental Biology 2019Quote: ... Cells were then washed 2 times in PBS and replaced with medium containing fresh SB431542 or 5ng/mL TGFβ1 (Peprotech, 100-21C) or 10ng/mL Activin A (Sigma Aldrich ...
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bioRxiv - Cancer Biology 2019Quote: ... Cultures were maintained in complete RPMI with 200-300 U/ml human recombinant IL-2 (PeproTech, Rocky Hill, NJ #200-02) and media was refreshed every 3 days ...
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bioRxiv - Neuroscience 2022Quote: ... then 4 million cells were seeded per well in 2 ml of EB Medium: complete mTeSR + 0.05 ug/ml BMP-4 (PeproTech, #120-05) + 0.05 ug/ml VEGF (PeproTech ...
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bioRxiv - Immunology 2024Quote: ... Transduced cells were incubated for 4 days with plate-bound anti-CD3/anti-CD28 in media supplemented with hIL-2 (0.0344 units/mL, Peprotech #200-02). Transduction efficiency was assessed for each experiment by flow cytometry.
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bioRxiv - Cancer Biology 2022Quote: ... PBMCs were harvested from K3EDTA anti-coagulated whole blood by density gradient centrifugation with Lymphoprep and plated in 12 well plates at 2-4 × 106 cells per well in 10% RPMI + 10 ng/mL human CSF (300-25, Peprotech, UK). Medium was replaced every 2-3 days until adherent populations of macrophages had established ...
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bioRxiv - Cell Biology 2023Quote: ... at a density of 0.15×106 cell/well and cultured for 2 days in supplemented α-MEM with 30 ng/ml M-CSF (PeproTech, UK). Media were replaced with α-MEM supplemented with 30 ng/ml M-CSF and 30 ng/ml RANKL (PeproTech ...
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bioRxiv - Immunology 2024Quote: ... 50 μM 2-mercaptoethanol) with 100 ng/ml of human FMS-like tyrosine kinase 3 ligand (FLT3L, PeproTech, Cranbury, NJ, USA) for 7 days ...
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bioRxiv - Genomics 2024Quote: ... HFFs treated with IFN were incubated with 550U/ml IFNα (PBL; 11200-2) and 700U/mL IFNβ (Peprotech; 300-02BC-20) and harvested after 5 hours ...
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bioRxiv - Immunology 2024Quote: ... China) gradient and pulsed with 0.5 µM rUreB protein in the presence of 5 U/ml rmIL-2 (PeproTech, NJ, USA). Cells were cultured in “RF-10” medium consisting of RPMI 1640 (Gibco ...
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bioRxiv - Developmental Biology 2020Quote: ... the culture medium was replaced with fresh DMEM/F12 with 2% FBS supplemented with TGFβ1/TGFβ3 (10 ng/ml; PeproTech EC, Ltd., London, UK) or 5mM citric acid (diluent for lyophilized TGFβ ...
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bioRxiv - Cell Biology 2019Quote: ... medium is made with DMEM/F12 together with the following components: 100 ng/mL Fetal Growth Factor-2 (FGF2 Peprotech #450-33), 100 ng/mL Epidermal Growth factor (EGF ...
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bioRxiv - Cancer Biology 2019Quote: ... Samples were examined by ELISA capturing with Fc-iNKG2D and detecting with biotinylated rabbit-anti-human IL-2 polyclonal antibody (Peprotech #500-P22BT) followed by incubation with streptavidin-HRP ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were deprived of serum for 2h and stimulated with 2 ng/ml of recombinant human EGF (Peprotech; Catalog #AF-100-15) for 2 ...
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bioRxiv - Bioengineering 2020Quote: ... 50 uL HS (250 mM NaCl) and PS (145 mM NaCl) gels (10 mg/mL fibrinogen) were fabricated with 100 ng/mL FGF-2 (Peprotech, 100-18b) and 100 ng/mL VEGF-165 (Shenandoah Biotechnology ...
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bioRxiv - Bioengineering 2020Quote: ... Primary human T cells were cultured in complete RPMI 1640 supplemented with 100 U/mL recombinant human IL-2 (PeproTech, 200-02). Cells were cultured at 37°C in a humidified 5% CO2 incubator.
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bioRxiv - Cancer Biology 2019Quote: ... in 200µl of RPMI supplemented with 2% FBS and 5% Matrigel with or without the addition of human IFN-γ (PeproTech; #300-02), mouse IFN-γ (PeproTech ...
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bioRxiv - Systems Biology 2020Quote: ... 2 mM glutamine and 10% fetal calf serum in the presence of 50 ng/ml of M-CSF (Peprotech, cat# 300-25). 5 × 105 CD14+CD16− monocytes or × 105 resting macrophages were activated with 10 ng/ml of lipopolysaccharides (LPS ...
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bioRxiv - Immunology 2021Quote: ... cells were starved for 6 hours in fresh media lacking cytokines and subsequently treated with 100 U/mL of either IL-2 or IL-15 (PeproTech 200-15).
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bioRxiv - Microbiology 2020Quote: ... Frozen activated PBMC cells were thawed and resuspended in complete PBMC media containing RPMI supplemented with 21 ng/mL human IL-2 (Peprotech; #200-02), 10% fetal bovine serum and 1% penicillin-streptomycin ...
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bioRxiv - Microbiology 2020Quote: ... Pellets were resuspended in activation medium containing RPMI supplemented with 21 ng/mL human IL-2 (Peprotech, Rocky Hill, NJ; #200-02), 1.5 μg/mL PHA (Remel ...
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bioRxiv - Immunology 2020Quote: ... cells were washed and cultured for a further 4 days in media containing 20 ng/mL recombinant human IL-2 (Peprotech, London, UK). For analysis of effector cytokine production ...
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bioRxiv - Developmental Biology 2022Quote: ... The cells were cultured on pre-coated plates with 0.5% growth factor-reduced Matrigel at a density of 2×104 cells/cm2 with MEF-conditioned medium supplemented with 10 ng/ml BMP4 (Peprotech, 120-05ET), 1µM A83-01 ...
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bioRxiv - Systems Biology 2020Quote: ... on Day 1 we changed the medium to RPMI + 2% B-27 (minus insulin) + 5ng/ml BMP4 (Peprotech, cat. AF-120-05ET) + 1uM Chiron 99021 and incubated these cells for 48 hours ...
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bioRxiv - Developmental Biology 2019Quote: ... From day 2 to day 4 the medium was additionally supplemented with BMP and TGFβ inhibitors (Noggin, Peprotech and SB431542, Tocris Bioscience). Treatment with MEK inhibitor (PD184352 ...
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bioRxiv - Bioengineering 2019Quote: ... 1 ng/mL human fiberblast growth factor-2 (hFGF2) and 0.25 ng/mL transforming growth factor-β1 (TGF-β1; all growth factors from PeproTech, Rocky Hill, NJ). Cells were treated with fresh medium every two days.
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bioRxiv - Pathology 2020Quote: ... HUVEC were plated at a density of 10,000 cells/96-well plate in EGM-2 supplemented with recombinant human TNF-α (300-01A, PeproTech EC Ltd), IL1-β (200-01B ...
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bioRxiv - Immunology 2019Quote: ... 50μM 2-ME and 10 ng/ml of recombinant murine granulocyte– macrophage colonystimulating factor (GM-CSF, PeproTech Inc., Rocky Hill, NJ, USA) for 7 days ...
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bioRxiv - Immunology 2021Quote: ... Activation was carried out at a cell density of 1M/ml in 100 U/ml Recombinant Murine IL-2 (Peprotech 212-12). Dynabeads™ Mouse T-Activator CD3/CD28 (ThermoFisher 11453D ...
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bioRxiv - Immunology 2022Quote: ... coated with anti-CD3 (4 μg/ml) and anti-CD28 (4 μg/ml) in the presence of IL-2 (Peprotech, 50ng/ml). CD19+ B cells were stimulated for 24 hours using Purified F(ab’)2 Goat anti-mouse IgM Antibody (BioLegend) ...
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bioRxiv - Immunology 2022Quote: ... same number of magnetically sorted naive T cells were co-cultured with equal numbers of BMDCs supplemented with 20 ng/ml of recombinant mouse IL-2 (PEPROTECH: 212-12) for 24-72 hours ...
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bioRxiv - Biophysics 2023Quote: ... the cells were stimulated at 37 °C for 15 min with 2 mL of 5 nM InlB321 variant (Table S1) or 1 nM HGF (#100-39H, PeproTech, Hamburg, Germany) in serum-free DMEM-F12 ...
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bioRxiv - Immunology 2023Quote: ... 55 uM 2-mercaptoethanol and 20 ng/ml of recombinant murine granulocyte-macrophage colony-stimulating factor (PeproTech, Rocky Hill, NJ, United States)] at 1:5 apoptotic cancer cell to cDC1 ratio ...
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bioRxiv - Immunology 2023Quote: All cell lines were seeded in flat 96-well plates at a density of 0.25 × 105 cells/well and cultured for 24 h in the absence or presence of human recombinant IFNγ and TNFα with activities of 2 × 107 Units/mg (PeproTech, Rehovot, Israel). Cells lines were then co-cultured with 100,000 (at density of 1×106 cell/ml ...
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bioRxiv - Systems Biology 2023Quote: B2B1 cells were seeded on tissue culture plastic (6-well to 15-cm plates) at 12,500 cells/cm2 in assay medium + 2% matrigel + 5 ng/ml EGF (Peprotech, AF-100-15) and refed every four days ...
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bioRxiv - Developmental Biology 2023Quote: ... cells were washed with PBS and further cultured for two days in S1/2 medium supplemented with FGF7 (50ng/ml) (PeproTech, 100-19) and vitamin C (0.25mM ...
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bioRxiv - Bioengineering 2023Quote: ... Primary human T cells were cultured in complete RPMI 1640 supplemented with 100□U/ml recombinant human IL-2 (PeproTech, 200-02). All mammalian cells were cultured at 37□°C in a humidified 5% CO2 incubator.
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bioRxiv - Immunology 2023Quote: ... TIL or KSQ-001 cells were added to spheroid plate in 100μL REP media supplemented with 6000IU/mL IL-2 (Peprotech, Cat#200-02). Images were taken via Incucyte S3 (Sartorius ...
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bioRxiv - Bioengineering 2024Quote: ... Isolated PB-NK cells were expanded with irradiated K562-mb15-41BBL feeder cells (1-3 days before NK cell isolation, 100 Gy) along with 50 IU/mL IL-2 (Peprotech, Cranbury, NJ) and 2 ng/mL IL-15 (Peprotech ...
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bioRxiv - Bioengineering 2024Quote: ... Cells were then moved to a flat-bottom 96 well plate containing 160 uL of medium supplemented with 500 U/mL IL-2 (Peprotech, Cranbury, NJ) or 10 ng/mL of IL-7 (BioTechne ...
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bioRxiv - Developmental Biology 2024Quote: ... DE cells were rinsed with PBS and then exposed to S1/2 medium supplemented with 50 ng/ml KGF (FGF7) (PeproTech, 100-19) and 0.25 mM vitamin C (VitC ...
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bioRxiv - Biochemistry 2024Quote: ... Each diluted suspension was carefully mixed and further diluted to 2 ml using OPC differentiation media (antibiotic-free DMEM-Sato supplemented with 10 ng/ml CNTF (Peprotech 450-13), 10 µM forskolin (Sigma-Aldrich F6886 ...
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Nanoparticle-delivered TLR4 and RIG-I agonists enhance immune response to SARS-CoV-2 subunit vaccinebioRxiv - Immunology 2022Quote: ... RBC-lysed murine bone marrow was seeded at 2 million cells/mL in complete RPMI media with 200 ng/mL human FLT3 ligand (PeproTech, Cat# 300-19) in a 6-well plate (10 million cells/well) ...
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bioRxiv - Physiology 2019Quote: ... Cells were then serum starved in DMEM culture media with 2% BGS and the conditioned culture was supplemented with 200ng/ml of murine IL-10 recombinant protein (Peprotech, Rocky Hill, NJ). After 48 hours ...
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bioRxiv - Cell Biology 2021Quote: The immature motor neurons were seeded on the cell imaging coverglasses in a low density (1.5×104 cells/cm2) and grown for 2 days in the fourth step medium supplemented with neurotrophic factor (NTF) cocktail: IGF1 (PeproTech, 10 ng/ml), CNTF (PeproTech ...
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bioRxiv - Immunology 2020Quote: ... 2 million bone marrow cells were co-cultured with 1 × 105 GL261 cells in 6-well plates in 2 ml RPMI/10% FBS supplemented with 40 ng/ml GM-CSF and 80 ng/ml IL-13 (PeproTech, Rocky Hill, NJ) for 3-4 days ...
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bioRxiv - Immunology 2019Quote: ... 50μM 2-ME and 100 ng/ml of recombinant murine fms-like tyrosine kinase-3 ligand (Flt3L, PeproTech Inc., Rocky Hill, NJ, USA) for 7days ...