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Citations for Peprotech :
851 - 900 of 941 citations for 6 Hydroxymethyl 4 methoxy 5 methyl nicotinic acid methyl ester since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2022Quote: ... qNSCs were treated with aNSC media with the addition of 0.5 μg/mL of the BMP-4 antagonist noggin (PeproTech 120-10C) to more reproducibly induce quiescence exit.
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bioRxiv - Immunology 2022Quote: ... Whole mouse splenocytes and human PBMCs were plated at a density of 5×106 cells per well in a Cepallet W-type 24 well microplate (DIC, Japan) and cultured for 4 days with or without mouse/human M-CSF (Peprotech, USA). Surviving macrophages adhered to 24-well plates were detached by lowering the temperature on ice ...
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bioRxiv - Cancer Biology 2022Quote: ... PBMCs were harvested from K3EDTA anti-coagulated whole blood by density gradient centrifugation with Lymphoprep and plated in 12 well plates at 2-4 × 106 cells per well in 10% RPMI + 10 ng/mL human CSF (300-25, Peprotech, UK). Medium was replaced every 2-3 days until adherent populations of macrophages had established ...
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bioRxiv - Immunology 2024Quote: ... together with 10 ng/mL recombinant mouse IL-4 or 3 μg/mL LPS with 10 ng/mL recombinant murine IFN-ψ (Peprotech) and 20 ng/mL recombinant human BAFF or 5 μg/mL anti-CD40 (Bio X Cell ...
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bioRxiv - Immunology 2024Quote: ... Transduced cells were incubated for 4 days with plate-bound anti-CD3/anti-CD28 in media supplemented with hIL-2 (0.0344 units/mL, Peprotech #200-02). Transduction efficiency was assessed for each experiment by flow cytometry.
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bioRxiv - Cancer Biology 2023Quote: ... with 100 μl of processed CM in the presence or absence of anti-LIF antibody (4 μg/ml) or recombinant mouse LIF (0.1 μg/ml; Peprotech, 250-02). Spheroids were quantified after 8 d culture.
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bioRxiv - Immunology 2024Quote: ... was used for sensitizing the cells in the presence of recombinant human IL-4 (Gibco, PeproTech, #200-04; 10 ng/mL) for three days ...
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bioRxiv - Cell Biology 2024Quote: ... centrifuged and resuspended in satellite cell growth medium (SCGM: F-10 Ham’s medium supplemented with 20% FBS, 4 ng/ ml FGF2 (PeproTech; 100-18B), 1% penicillin–streptomycin and 10mM HEPEs) ...
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bioRxiv - Neuroscience 2022Quote: ... coated 6 well plates at 200,000 cells/well in 2 ml/well StemFlex supplemented with ROCK inhibitor Y-27632 (5 mM, PeproTech) to inhibit apoptosis ...
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bioRxiv - Cell Biology 2022Quote: ... The cells were cultivated on 0.2 % gelatine-coated plates and were maintained in proliferation medium supplemented with 5 ng/ml bFGF (Peprotech) every second day (Rahman et al. ...
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bioRxiv - Immunology 2021Quote: ... (2) treated with recombinant mouse IL-12 (5 ng/ml) and IL-18 (25 ng/ml, both from Peprotech), (3 ...
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bioRxiv - Immunology 2023Quote: ... at a 1:1 bead:cell ratio in complete RPMI supplemented with 5 ng/mL IL-7 and IL-15 (both PeproTech) and after 48-72 h ...
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bioRxiv - Cell Biology 2024Quote: ... was induced by 1-3 days of 2.5 µM CHIR99021 (Stemgent) and 3-5 days of 100 ng/ml Activin A (Peprotech) in RPMI medium ...
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bioRxiv - Neuroscience 2021Quote: ... Cells were dissociated using 5mM EDTA in HBSS and resuspended in media with B-27 supplement for 4 hours before incubation with recombinant BDNF protein (100nM, Peprotech, #450-02) or vehicle (equal volume of 0.1% BSA in H2O) ...
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bioRxiv - Immunology 2020Quote: ... cells were washed and cultured for a further 4 days in media containing 20 ng/mL recombinant human IL-2 (Peprotech, London, UK). For analysis of effector cytokine production ...
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bioRxiv - Immunology 2020Quote: ... Alternately M2 MΦs were activated as follows: M2a MΦs from stimulation with 20 ng/mL recombinant human IL -4 (PeproTech; Rock Hill, NJ, USA) and 25 ng/mL recombinant human IL-13 (PeproTech ...
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bioRxiv - Neuroscience 2023Quote: ... this medium was switched to N2 medium (Advanced DMEM/F-12, 4% FBS, 1% N2, 1% Penicillin-Streptomycin) supplemented with 10 ng/mL CNTF (PeproTech, 450-13). After 12 days ...
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bioRxiv - Cancer Biology 2023Quote: ... cells were cultured in the absence of EGF and FGF and with the addition of either 10 ng/mL BMP-4 (Peprotech; #315-27) or 1% or 2% foetal bovine serum (Gibco ...
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bioRxiv - Neuroscience 2023Quote: ... cells were expanded in N2 medium (Advanced DMEM/F-12, 4% FBS, 1% N2, 1% Penicillin-Streptomycin) supplemented with 10 ng/mL CNTF (PeproTech, 450-13), which was switched to 10 ng/mL EGF after 12 days ...
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bioRxiv - Immunology 2023Quote: ... in the presence of 40 ng/ml of granulocyte-macrophage colony-stimulating factor (GM-CSF) and IL-4 (both from Peprotech or Biolegend). Cells were cultured in 24-well plates in DMEM (Gibco) ...
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bioRxiv - Systems Biology 2024Quote: ... Osteogenic differentiation was initiated on Day 0 by culturing in osteogenic differentiation medium consisting of growth medium and 100 ng/mL bone morphogenetic protein-4 (BMP4) (PeproTech, 315-27). The osteogenic differentiation medium was replaced every second day.
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bioRxiv - Neuroscience 2024Quote: ... OPCs from each animal were expanded in 3x 175cm2 flasks for 3-4 days in the presence of 10ng/mL platelet derived growth factor-aa (PDGFAA, Peprotech 100-13A). Cells were harvested fresh for each round of experiments and used at the time of first passage ...
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bioRxiv - Immunology 2022Quote: Cells were cultured in complete T cell media supplemented with 5 ng/mL each of recombinant human IL-7 and IL-15 (PeproTech) and 10 U/mL recombinant human IL-2 ...
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bioRxiv - Immunology 2022Quote: Cells were cultured in complete T cell media supplemented with 5 ng/mL each of recombinant murine IL-7 and IL-15 (PeproTech). One day prior to activation of OT-I cells ...
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bioRxiv - Neuroscience 2021Quote: ... The isolated OPCs were then cultured on PDL-coated plates using GM plus 5 ng/ml FGF (450-33, Peprotech), 4 ng/ml PDGF-AA (315-17 ...
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bioRxiv - Cell Biology 2021Quote: Immunofluorescent staining was in general performed on HUVECS cultured on 12 mm glass coverslips coated with 5 μg/ml FN and treated with or without o/n TNFα (10ng/ml) (Peprotech), washed with PBS+/+(1mM CaCl2 and 0.5mM MgCl2) ...
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bioRxiv - Neuroscience 2022Quote: ... astrocyte cultures at 80% confluence in 6-well plates (in E6 medium containing BMP4 and FGF2) were treated with vehicle control or IL-1a (5 ng/ml; Peprotech), TNFα (10 ng/ml ...
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bioRxiv - Immunology 2022Quote: ... for Th1 polarization the medium was supplemented with 5 ng/ml IL-2 and 10 ng/ml IL-12 (both from Peprotech), and 10 μg/ml anti-IL-4 (11B11 ...
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bioRxiv - Cancer Biology 2022Quote: ... mAb in 200ul of T cell media supplemented with 5 ng/ml of IL-7 and 30 U/ml of IL-2 (PeproTech) for 4 and 7 days ...
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bioRxiv - Immunology 2020Quote: ... adherent monocytes were differentiated into macrophages over a 5 days in the presence of 50 ng/mL GM-CSF (Peprotech) in RPMI supplemented with 10% foetal calf serum (FCS ...
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bioRxiv - Immunology 2020Quote: ... 5 ng/ml of IL-2 was added to the culture and memory T cells were supplemented with 5 ng/ml IL-7 and IL-15 (all Peprotech). Cells were activated in a reverse time-course so that their total time in culture was identical and so that all cells would finish their indicated activation period at the same day to enable extracellular flux analysis ...
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bioRxiv - Genomics 2020Quote: ... 1% Glutamax, and 1% FBS, 8 ng/ml FGF2, 5 ng/ml CNTF, and 10 ng/ml BMP4, from Peprotech). On day 7 ...
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bioRxiv - Genetics 2020Quote: ... and plated on Matrigel-coated plate in a density of 5×104 cells cm-2 in ES cell medium containing 10 ng/mL fibroblast growth factor 2 (FGF2, Peprotech). The differentiation was started by replacing ES cell medium with KSR medium and gradually switched to N2 medium from day 4 to day 10 ...
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bioRxiv - Immunology 2020Quote: Adherent PBMC monolayer was washed twice with HBSS and monocytes were differentiated into hMDMs for 5 days in RPMI 1640 containing 100 ng/mL macrophage colony-stimulating factor (M-CSF, PeproTech) and 10% foetal calf serum ...
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bioRxiv - Developmental Biology 2020Quote: ... treated with 5 ng/ml recombinant (r) human TGFβ1 derived from HEK293 cells (100-21, PeproTech, Rocky Hill, NJ, USA) for 1 ...
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bioRxiv - Bioengineering 2022Quote: ... cells were incubated with StemPro-34 medium from days 2 to 5 with a cocktail of growth factors: vascular endothelial growth factor (VEGF; PeproTech), BMP-4 ...
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bioRxiv - Cancer Biology 2022Quote: ... Enriched cells were taken into culture in T cell medium (TCM, see Methods – primary human material and cell lines) supplemented with 5 ng/mL IL-7 (Peprotech), 5 ng/mL IL-15 (Peprotech) ...
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bioRxiv - Immunology 2022Quote: ... mice were repeatedly immunized every 14 days (5 times) subcutaneously (s.c.) into the flank with 10μg of human TNFR2 protein (Peprotech, 310-12) and 20μg MPLA (Sigma ...
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bioRxiv - Immunology 2024Quote: ... cells were thawed and rested in an 37°C / 5% CO2 incubator in the T cell medium with 50 U/mL rhIL-2 (Peprotech, Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2024Quote: ... at 3:1 bead: T-cell ratio together with human recombinant IL-15 and IL-7 (PeproTech; 5 ng/mL) in AIM-V medium supplemented with 5% FBS ...
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bioRxiv - Immunology 2023Quote: ... supplemented with 5% heat-inactivated fetal bovine serum (Biosera FB-1001) and 50ng/ml recombinant M-CSF (Peprotech 300-25). Differentiated macrophages were plated at a density of 1 x 106ml in the appropriate cell culture plate at least 1 d prior to stimulation.
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bioRxiv - Immunology 2023Quote: ... Isolated T cells were otherwise activated using plate bound anti-CD3 (5 μg/ml, inVIVOMAb Cat# BE0002) in complete medium added with 100 U/ml IL-2 (Peprotech) and soluble αCD28 (0.5 μg/ml ...
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bioRxiv - Immunology 2023Quote: ... splenic NK cells were MACs enriched and cultured at 37°C and 5% CO2 in RPMI containing 300U rIL-2 (Peprotech), 50ng/ml rIL-15 (Peprotech ...
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bioRxiv - Neuroscience 2023Quote: ... diluted and seeded on Poly-D-lysine – Matrigel coated coverglasses in a 12 well plate at the concetration of 5×104 cells per well in the Patterning medium [N2B27 medium containing 1ng/mL GDNF (Peprotech), 2ng/ml BDNF (Pepotech) ...
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bioRxiv - Genetics 2023Quote: ... and the cells were washed with phosphate buffered saline (PBS) and cultured in M231 SMDS medium supplemented with 5 ng/mL PDGF-BB (Peprotech) to provoke cell migration ...
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bioRxiv - Developmental Biology 2024Quote: ... and 106 cells were transferred onto each retronectin/DL1-coated virus-bound well supplemented with 5 ng/mL mSCF (Peprotech), 5 ng/mL Flt3-L ...
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bioRxiv - Developmental Biology 2024Quote: ... All in vitro T cell generation cultures were supplemented with 5ng/mL Flt3-L and 5 ng/mL IL-7 (Peprotech). Experiments involving in vitro generation of NK and ILC populations (Figures 3 ...
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bioRxiv - Cell Biology 2024Quote: ... 5 µM A83-01 without EGF (HOME0) or with 0.1-50 ng/mL human recombinant EGF (Peprotech, Cranbury, NJ, USA) (HOME0.1 ...
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bioRxiv - Immunology 2024Quote: ... For Th1 polarization T lymphocytes were cultured with 5 ng/ml of recombinant murine IL-12 (Peprotech, catalog 210-12). For stimulation of preactivated T lymphocytes with type I IFN ...
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bioRxiv - Neuroscience 2024Quote: ... Cells were then centrifuged at 300g for 5 minutes and resuspended in 2 ml of NDM-A medium supplemented with 20 ng/ml BDNF (Peprotech) and plated onto poly-L-ornithine/laminin-coated dishes at a density of 80,000 cells per cm2 ...