Labshake search
Citations for Peprotech :
801 - 850 of 2075 citations for Recombinant Human PPAR gamma Protein His tag since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... diluted in complete RPMI (cRPMI; RPMI supplemented with 10% FBS plus recombinant human IL-2 (20IU/ml; Peprotech), as previously described.65,66 Activated cells were incubated for six days with a media change on day three.
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bioRxiv - Immunology 2021Quote: ... Inducers of neutrophil chemotaxis were added to the bottom well in 600 µL of RPMI-1640 media containing 1,000 or 50,000 pg of recombinant human (rh-) IL-8 (PeproTech) or A549 cells culture previously infected for 48 hours with mock or ZIKV PE243 (1 MOI) ...
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bioRxiv - Immunology 2022Quote: ... seeded at a density of 0.5×106/mL and stimulated with 0.5 or 25 ng/ml recombinant human IL-15 (PeproTech,). After an overnight period ...
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bioRxiv - Cell Biology 2024Quote: ... China) supplemented with 50 ng/mL recombinant human fibroblast growth factor 10 (FGF-10; 100-26, PeproTech, USA), 3 μM CHR99021 (SML1046 ...
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bioRxiv - Immunology 2023Quote: ... and expanded in RPMI 1640 containing 10% FBS and 100 IU/mL of recombinant human IL-2 (Peprotech). Human blood samples were enriched for T cells using negative bead selection (StemCell ...
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bioRxiv - Bioengineering 2024Quote: ... 20 ng/mL interleukin-4 (IL-4) and 50 ng/mL recombinant human M-CSF (Peprotech EC Ltd.) were added to the culture media for 24 hours ...
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bioRxiv - Bioengineering 2022Quote: ... 1 ng/mL recombinant human fibroblast growth factor (FGF-basic 154 a.a., PeproTech #100-18B, Cranbury, NJ, USA), 20 mM HEPES pH 7.4 (Sigma Aldrich #H4034 ...
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bioRxiv - Immunology 2022Quote: ... Approximately 2.5×105 TDM and MDM were stimulated with recombinant human IL-6 (200 or 2000 pg, Peprotech) for 24 hours at 37ºC and 5% CO2 ...
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bioRxiv - Cell Biology 2024Quote: ... EGF stimulation was obtained with 10 ng/ml of animal-free recombinant human EGF (Peprotech AF-100-15) for 3h ...
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bioRxiv - Molecular Biology 2024Quote: ... 0.2 μg/mL recombinant human relaxin-2 in 0.1% BSA-PBS (Rln, R; Peprotech, Rocky Hill, NJ, USA) and a combination of E+R for the specified amount of time ...
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bioRxiv - Physiology 2024Quote: ... Inflammatory cocktail treatment was carried out by adding recombinant human TNFalpha (Peprotech #300-01A, final concentration 100ng/mL), IL-1beta (Peprotech # 200-01B ...
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bioRxiv - Immunology 2024Quote: ... Cells were cultured in NK MACS medium (Miltenyi) supplemented with recombinant human IL-2 1000 U/mL (Peprotech). Cells were activated over 7 days with anti-CD2 and anti-NKp46-coated beads (Miltenyi ...
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bioRxiv - Cell Biology 2024Quote: ... cells were cultured in HAM’s F10 medium supplemented with 20% FBS and 2.5 ng/ml recombinant human FGF (PeproTech). When indicated ...
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bioRxiv - Cancer Biology 2024Quote: ... MO-LAS-B cells were stimulated with recombinant human IL-13 (200-13, PeproTech, Rocky Hill, NJ, USA) at 10 ng/mL or left unstimulated (control) ...
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bioRxiv - Neuroscience 2023Quote: ... or with 50 ng/ml interferon gamma INF-γ (Peprotech, London, UK), 1ug/ml Resiquimod (R848) ...
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bioRxiv - Cancer Biology 2022Quote: ... Cells were afterwards stimulated with indicated amount of recombinant protein CXCL2 (250-15-20, PeproTech GmbH) in DMEM medium without FCS or conditioned medium of endothelial cells.
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bioRxiv - Cancer Biology 2021Quote: ... Recombinant IFN-γ (300-02) and anti-human IFN-γ antibody (506532) were purchased from PeproTech (Rocky Hill, USA). Etoposide (HY-13629 ...
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bioRxiv - Developmental Biology 2020Quote: Small molecules and cytokines were supplemented as indicated in screening related figure legends: recombinant human BMP4 (10ng/ml, Peprotech), BMPRi (LDN193189 0.3µM ...
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bioRxiv - Developmental Biology 2020Quote: ... or C57BL6/129sJae EpiSC line (derived following in vitro priming of V6.5 mouse ESCs) were expanded in N2B27 with 12ng/ml recombinant human bFGF (Peprotech) and 20ng/ml recombinant Human ACTIVIN A (Peprotech) ...
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bioRxiv - Genomics 2020Quote: ... cells were assessed with and without 16h pretreatment of 10 ng/ml recombinant human IFNγ (Peprotech, Rocky Hill, NJ).
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bioRxiv - Biochemistry 2020Quote: ... according to the manufacturer’s instructions and cultured in RPMI supplemented with 10% FBS and 30 U/ml recombinant human IL-2 (PeproTech) at 37°C in 5% CO2 ...
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bioRxiv - Cancer Biology 2022Quote: ... with sorted autologous TFH cells (at a 1:1 ratio) or TFH mimicking signals (including 0.02 μg/mL recombinant human IL-4, Peprotech; 0.05 μg/ml recombinant human IL-21 ...
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bioRxiv - Biochemistry 2022Quote: ... lung organoids were cultured in medium including recombinant human transforming growth factor (TGF)-β1 (Peprotech, Rocky Hill, NJ, USA) without A83-01 (Tocris ...
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Variation in Leishmania chemokine suppression driven by diversification of the GP63 virulence factorbioRxiv - Microbiology 2021Quote: ... normalized live parasites (1×106) or heterologously expressed GP63 was incubated with 500pg/ml of human recombinant CXCL10 (Peprotech) for the indicated time at 37°C ...
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bioRxiv - Neuroscience 2021Quote: ... organoids were also generated as described excepting the addition of 25ng/mL recombinant human PTN (Peprotech, CAT#: 450-15) into differentiation media for 7d.
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bioRxiv - Immunology 2021Quote: ... at a bead to cell ratio of 3:1 and 1,000 U/mL of recombinant human IL-2 (Peprotech). Fresh IL-2 containing media was added every 48 h and Human T-activator CD3/CD28 Dynabeads were added again at day 7 and removed at day 15 of expansion ...
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bioRxiv - Bioengineering 2024Quote: ... SCF (20 ng/ml) and TPO (25 ng/ml) (all recombinant human, Peprotech, 300-19, 300-07, 300-18) (100% medium) ...
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bioRxiv - Immunology 2024Quote: CD8 T cells from WT p14 or Irf5-/- p14 mice were isolated as described above and cultured with or without 1µg/ml gp33 peptide in complete RPMI medium supplemented with 2ng/ml recombinant human IL-2 (Peprotech). Cells were then collected at 6 or 24 hours after incubation and washed with PBS ...
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bioRxiv - Immunology 2023Quote: ... tumor suspension and PBMCs were thawed and plated with recombinant human IFNy at 200 ng/mL (Peprotech, #300-02) (only for tumor suspension ...
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bioRxiv - Immunology 2023Quote: ... at a density of 7.5×105 cells/ml in 2 ml complete media supplemented with 20 ng/ml recombinant human GM-CSF (Peprotech). The plates were incubated at 37°C in 5% CO2 ...
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bioRxiv - Bioengineering 2024Quote: ... naive primary human CD4+ T cells were cultured on-chip for 24 h with media spiked with 1.22 ng/mL of recombinant human CCL21 (Peprotech). Brightfield images were acquired using transmitted light in time series mode (1 image was collected every 30 sec for 10 cycles ...
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bioRxiv - Immunology 2023Quote: ... in the presence of 2mg/mlaCD28 (clone 37.51, WEHI antibody facility) and 100U/ml recombinant human IL-2 (Peprotech), in 96 well flat-bottom plates at 104 cells per well ...
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bioRxiv - Biochemistry 2024Quote: ... and 50 µM β-mercaptoethanol (BME)) supplemented with 100 U/mL recombinant human IL-2 (Peprotech Cat# 200-02). T cells were seeded at 3×105 cells per well of a pre-coated 24-well culture plate in 1.2 mL of TCM ...
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bioRxiv - Immunology 2024Quote: ... Samples were either left unstimulated or were activated by adding 500ng/mL of recombinant human IFNγ (PeproTech 300-02) for 24 hours ...
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bioRxiv - Immunology 2024Quote: ... Cells were then split for experiments and treated with or without 20 ng/ml recombinant human TGF-β1 (PeproTech) prior to activation.
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bioRxiv - Immunology 2024Quote: ... followed by culturing in exosome-free media (EFM) supplemented with 20 ng/mL of recombinant human IL-4 (Peprotech) to produce IL-4 conditioned media (19).
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bioRxiv - Immunology 2022Quote: Cells were cultured in complete T cell media supplemented with 5 ng/mL each of recombinant human IL-7 and IL-15 (PeproTech) and 10 U/mL recombinant human IL-2 ...
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bioRxiv - Microbiology 2020Quote: ... the PBMCs were cultured with the synthetic peptide mixture of SARS-CoV-2 at a concentration of 1μg/ml or DMSO in RPMI 1640 medium containing 10% FCS and 20 U/ml recombinant human IL-2 (Peprotech) for 7 days ...
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bioRxiv - Microbiology 2020Quote: ... were cocultured with 2×105 peptide-pulsed autologous DCs in a 24-well plate in the presence of 10 ng/mL recombinant human interleukin-2 (IL-2; Peprotech). The next day ...
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bioRxiv - Bioengineering 2021Quote: ... and vascular endothelial growth factor (VEGF) (Catalog No. 100-20, Recombinant Human VEGF165, Peprotech, Rocky Hill, NJ, 0.1 μg/mL). A mixture of heparin ...
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bioRxiv - Bioengineering 2021Quote: Lyophilized 90 % PEGDMA microrods were loaded by resuspending ∼100,000 (100 k) microrod aliquots in 20 μL of 1 mg/mL recombinant human β-NGF (Peprotech) as described in the Protein loading of PEGDMA microrods section ...
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bioRxiv - Cancer Biology 2021Quote: ... 106 anti-CD19 CD8 CAR T cells were expanded in presence of 7 x 106 irradiated (8000 cGy) TM-LCL feeder cells in RPMI1640 containing 50 IU/mL human recombinant IL-2 (Peprotech), 10 ng/ml recombinant human lL-15 (Peprotech) ...
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bioRxiv - Biochemistry 2022Quote: ... HeLa cells at a plate confluence of 80% were treated for 10 min with 100 ng/mL animal-free recombinant human EGF (PeproTech) or Gibco™ distilled water (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2022Quote: ... The isolated cells were cultured in RPMI medium supplemented with 20% (v/v) FBS and 100 ng/mL human recombinant IL-2 (Peprotech). Two days prior to transduction ...
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bioRxiv - Microbiology 2022Quote: ... 1 × 106 BlaER1 cells per well of a 6-well tissue culture plate were treated with 10 ng/ml human recombinant M-CSF and IL-3 (PeproTech) and 100 nM β-estradiol (Sigma-Aldrich ...
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bioRxiv - Microbiology 2020Quote: ... The cells were cultured in RPMI 1640 (Nissui) supplemented with 10% heat-inactivated calf serum (Biowest) and 1 ng/mL recombinant human interleukin 6 (IL-6, PeproTech). HeLa and 293T cells were cultured in DMEM (Nacalai tesque ...
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bioRxiv - Immunology 2022Quote: ... After a one hour resting period cells were transferred to 24-well plates and incubated for three days in medium containing 10U/mL recombinant human IL-2 (Peprotech) to allow establishment of the knockout ...
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bioRxiv - Physiology 2022Quote: ... NRK-49 cells whose culture medium was supplemented with 400pg/mL of recombinant human IL-1β (PeproTech, Cranbury, NJ, USA) and 1×10-7M Angiotensin II acetate human (Sigma) ...
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bioRxiv - Systems Biology 2020Quote: ... Cells were grown to at least 500,000 cells/mL before treatment with 20 ng/mL recombinant human tumor necrosis factor α (TNF) (Peprotech), 300 nM A-485 (Structural Genomics Consortium) ...
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bioRxiv - Microbiology 2020Quote: ... On DIV 10 add 100% N2 medium plus recombinant human Brain Derived Neurotrophic Factor 10ng/ml (BDNF 450-02; Peprotech); Glial-Derived Neurotrophic Factor 10ng/ml (GDNF 450-10 ...