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Citations for Peprotech :
751 - 800 of 1101 citations for SUN domain containing protein 2 SUN2 Antibody since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: The differentiation of isolated monocytes into macrophages was performed in RPMI 1640 GlutaMAx medium with 10% FCS and 1% P/S containing 100 ng/mL macrophage colony-stimulating factor (M-CSF) (PeproTech) for 7 days ...
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bioRxiv - Immunology 2020Quote: ... These cells were cultured for 7 d in DMEM cell culture medium containing 40 ng/ml macrophage colony-stimulating factor (M-CSF) (PeproTech). For the generation of bone marrow-derived dendritic cells ...
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bioRxiv - Molecular Biology 2021Quote: ... and 3 x 106 cells were plated to grow in suspension in 90 mm bacterial petri dishes containing N2B27 + Dox media supplemented with 10 ng/mL EGF and FGF (Peprotech). On day 10 ...
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bioRxiv - Immunology 2021Quote: Bone marrow cells were isolated from C57BL/6J mouse tibia and femur and then cultured for 7–9 days in 10% FBS DMEM containing mouse GM-CSF (50 ng/mL, Peprotech).
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bioRxiv - Molecular Biology 2021Quote: ... in 96-well plates (5.0 x 105 cells/well) and incubated with DMEM containing 10% FCS in the presence of 100 ng/ml recombinant murine RANKL (PeproTech) with 20 ng/ml recombinant murine M-CSF (PeproTech) ...
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bioRxiv - Immunology 2021Quote: ... Activated C7 cells were concentrated and 4 ml of culture was resuspended in 1 ml RPMI+ containing 10 μg/mL ESAT61-20 peptide + 20 ng/mL IL-12 (Peprotech) + 20 ng/mL IL-2 (Peprotech) ...
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bioRxiv - Immunology 2020Quote: ... the obtained bone marrow cells were cultured in RPMI-1640 containing 10% FBS and 10 ng mL−1 mouse macrophage colony-stimulating factor (M-CSF, Peprotech) in a humidified 5% CO2 atmosphere at 37 °C for six days ...
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bioRxiv - Immunology 2020Quote: ... approximately 2 x 106 cells were then seeded per 100 mm plate in 10 ml of media containing 20 ng/ml of recombinant murine (rm) GM-CSF (Peprotech). On day 3 ...
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bioRxiv - Cell Biology 2021Quote: ... bone marrows were cultured and in vitro differentiated in bone marrow differentiation medium containing 40 ng/ml recombinant murine M-CSF (PeproTech) as described before (Sun et al. ...
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bioRxiv - Pathology 2023Quote: ... Bone marrow was collected and cultured in RPMI-1640 complete medium (CM) containing 20ng/ml recombinant mouse GM-CSF (Peprotech). Subsequently ...
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bioRxiv - Cancer Biology 2023Quote: ... and conditioned media containing approximately 100 ng/ml SCF for the generation of neutrophil-biased cells or GM-CSF 10 ng/ml (Peprotech) for the generation of macrophage progenitors ...
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bioRxiv - Neuroscience 2023Quote: ... Neuronal cultures (as above) were enriched for astrocytes via continuous EDTA passaging in N2B27 media containing 10ng/ml FGF2 (Peprotech). At 150 days in vitro ...
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bioRxiv - Cancer Biology 2023Quote: ... CD14+ monocytes were cultured for 7 days in Roswell Park Memorial Institute media containing 10-ng/mL human granulocyte-macrophage colony-stimulating factor and 10-ng/mL human IL-6 (PeproTech). For cell binding competition with AL009 ...
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bioRxiv - Neuroscience 2023Quote: ... diluted and seeded on Poly-D-lysine – Matrigel coated coverglasses in a 12 well plate at the concetration of 5×104 cells per well in the Patterning medium [N2B27 medium containing 1ng/mL GDNF (Peprotech), 2ng/ml BDNF (Pepotech) ...
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bioRxiv - Molecular Biology 2022Quote: ... MA) or sterile PBS containing 0.01% Tween-20 immediately followed by treatment media with or without TNFα (Peprotech, Cranbury, NJ) for 15 min ...
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bioRxiv - Cell Biology 2024Quote: ... and cultivated in 4 mL of B-cell medium containing either 10 ng·mL−1 rIL-4 or 10 ng·mL−1 rIL-21 (Peprotech, 210-21). On day 8 ...
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bioRxiv - Genomics 2023Quote: ... and 3 x 106 cells were plated to grow in suspension in 90 mm bacterial petri dishes containing N2B27 media supplemented with 1 μg/ml doxycycline and 10 ng/ml EGF and FGF (Peprotech). Embryoid-body-like aggregates were collected by mild centrifugation (100 g for 2 min ...
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bioRxiv - Genomics 2023Quote: ... CD34+ HSPCs were differentiated to the megakaryocyte lineage by culturing for 11 days in IMDM based media containing 30ng/mL rhTPO (PeproTech), 1ng/mL rhSCF (PeproTech) ...
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bioRxiv - Neuroscience 2023Quote: ... Through days 13 to 28 the organoids were maturated in medium II containing 20 ng/mL BDNF (Peprotech, 450-02), 20 ng/mL GDNF (Peprotech ...
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bioRxiv - Immunology 2023Quote: ... Cultures were changed on day 4 to new complete RPMI 1640 medium containing recombinant murine IL-5 (10 ng/ml, Peprotech) and further fed with IL-5 every other day between days 10 to 14 ...
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bioRxiv - Bioengineering 2022Quote: ... and then for three to five days (plasmablast/plasma cell differentiation) in medium containing 50 ng/mL IL-6 (Peprotech),10 ng/mL IL-15 and 15 ng/mL interferon-α2B (Sigma-Aldrich).
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bioRxiv - Cell Biology 2022Quote: ... The monolayer was washed 3 times with HBSS to remove non-adherent cells and monocytes were differentiated into macrophages for 3 days in RPMI media + 10% FBS containing 30 ng/ml M-CSF (PeproTech) and for further 4 days in media containing 30 ng/ml RANKL (PeproTech ...
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bioRxiv - Immunology 2022Quote: ... and resuspended in RPMI 1640 containing 20% FBS and 50 ng/ml human macrophage colony-stimulating factor (M-CSF) (300-25, Peprotech). Cells were seeded into sterile tissue-culture plates and differentiated for 7 days ...
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bioRxiv - Microbiology 2022Quote: ... Cells were cultured in RPMI 1640 medium with 10% FBS containing GM-CSF (25 ng/ml; Peprotech, Rocky Hill, NJ) and IL-4 (10 ng/ml ...
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bioRxiv - Bioengineering 2023Quote: ... After 2 days the coculture medium was replaced by coculture medium additionally containing 50 ng/mL receptor activator of NFκ-B ligand (RANKL, 310-01, PeproTech). Medium was changed weekly inside the incubator via the self-healing injection ports by removing 3 mL of the total 6 mL and replacing it by 3 mL fresh coculture medium with double the concentration of dexamethasone ...
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bioRxiv - Immunology 2023Quote: ... The transfection mix was then added dropwise to 2.5×106 M0 macrophages (pre-cultured for 6 days in a 6-well plate in antibiotic-free RPMI1640 macrophage media containing M-CSF (50ng/ml, Peprotech) – with media change on d3) ...
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bioRxiv - Immunology 2024Quote: ... Cells were plated in DMEM containing 10% v/v FBS and 10 ng/ml recombinant M-CSF (Peprotech #315-02). Cells were plated at a concentration of ∼150,000 cells per cm2 on non- treated Petri plates ...
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bioRxiv - Immunology 2024Quote: ... 3×105 CD14+ monocytes were seeded in a 12-well plate in 1 mL complete medium containing 20 ng/mL GM-CSF (#300-03; Peprotech) or 50 ng/mL M-CSF (#300-25 ...
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bioRxiv - Immunology 2024Quote: ... Cells were then resuspended in RPMI 1640 media containing 30 ng/ml GM-CSF and 20 ng/ml recombinant human TGF-β1 (PeproTech) and plated in untreated 48- or 24-well plates ...
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bioRxiv - Immunology 2024Quote: ... or unstained PBMCs were seeded at low density on a plasma-treated chip containing cIMDM supplemented with 10 ng/ml IL-15 (PeproTech) and 100 U/ml IL-2 (PeproTech) ...
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bioRxiv - Immunology 2024Quote: ... splenocytes were adjusted to a concentration of 3 x 106 cells/mL in T cell medium containing 10 ng/mL rhIL-7 (Peprotech) and 2 µg/mL anti-mouse CD28 (clone 37.51 ...
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bioRxiv - Immunology 2024Quote: ... the tibia and femur bones were flushed in ice-cold RPMI containing 10% FCS buffer with 10 ng/mL IL6 (216-16, Peprotech). An RBC lysis step was performed in 4 mL ammonium-chloride-potassium lysis buffer for 1 min at room temperature and subsequently inactivated with 26 mL RPMI containing 10% FCS ...
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bioRxiv - Immunology 2024Quote: ... 8X106 cells were seeded in 6 mL of DMEM containing 10% FBS and 50 ng/mL MCSF (PeproTech #315-02) in a 100mm plate on day 0 ...
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bioRxiv - Immunology 2024Quote: Aliquots of approximately 15,000 pre-cDCs were then cultured for 4 days in a total volume of 150μl in flat-bottomed 96-well plates (Costar, #3596) containing complete RPMI medium supplemented with 20ng/mL GM-CSF (Peprotech) and 400ng/mL Flt3L (Peprotech) ...
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bioRxiv - Immunology 2024Quote: ... 100 units/mL penicillin and 100 μg/mL streptomycin] containing IL-4 (20 ng/mL) and GM-CSF (20 ng/ml) (PeproTech) and cultured for 5d with replacement of half of the medium and addition of fresh cytokines every other day ...
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bioRxiv - Neuroscience 2024Quote: ... CD11b and CD45 were derived from mesodermally-patterned embryoid bodies cultured in myeloid precursor media containing 100 ng/ml M-CSF (Peprotech) and 25 ng/ml IL-3 (Gibco ...
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bioRxiv - Immunology 2024Quote: ... 100 μg/mL streptomycin) containing 30 ng/mL of mouse granulocyte-macrophage colony-stimulating factor (GM-CSF) and Interleukin-6 (IL-6) (PeproTech). After 5 days ...
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bioRxiv - Immunology 2024Quote: ... 100 μg/mL streptomycin) containing 30 ng/mL of mouse granulocyte-macrophage colony-stimulating factor (GM-CSF) and Interleukin-6 (IL-6) (PeproTech) and 4% CHO-SCF conditioned media ...
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bioRxiv - Developmental Biology 2024Quote: ... The plate was placed in 37°C incubator for 60 minutes to solidify the Matrigel and then overlaid with basic differentiation medium containing 50 ng ml−1 of GDNF (PeproTech), 50 ng ml−1 of FGF10 (PeproTech) ...
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bioRxiv - Developmental Biology 2024Quote: ... and containing EpiLC medium: N2B27 medium (see composition above) supplemented with 20ng/ml Human Activin A (PeproTech, cat. 120-14E), 12ng/ml Human Fgf basic (R&D Systems ...
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bioRxiv - Immunology 2021Quote: ... we injected 32 μg anti-CCL5 antibody (500-P118, Peprotech) in 200 μl PBS per mouse intraperitoneally ...
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bioRxiv - Immunology 2023Quote: ... and polyclonal biotinylated rabbit anti-RELMα detection antibodies (all Peprotech) were used according to a previously described protocol [38].
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bioRxiv - Immunology 2021Quote: ... 100– 200 U/mL recombinant human IL-2 (PeproTech, Rocky Hill, New Jersey, USA), and a final concentration of 12.5% horse serum and 12.5% fetal bovine serum.
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bioRxiv - Genetics 2020Quote: ... Stimulation was performed for 4 days using recombinant human IL-2 (10ng/ml, Peprotech) and Anti-Biotin MACSiBead Particles loaded with CD2 ...
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bioRxiv - Synthetic Biology 2021Quote: ... and 2 ng/mL recombinant human granulocyte macrophage colony-stimulating factor (GM-CSF; PeproTech) unless specified otherwise ...
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bioRxiv - Bioengineering 2021Quote: ... injection and mice were dosed with 1×104 units recombinant human IL-2 (Peprotech) twice daily for 3 days (6 total injections) ...
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bioRxiv - Immunology 2021Quote: ... 1% penicillin- streptomycin-glutamine and 25 units/mL of IL-2 (Peprotech, #200-02), exchanging half the medium every 2-3 days ...
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bioRxiv - Cell Biology 2020Quote: ... Engineered skeletal muscles were maintained in GM with 5ng/mL FGF-2 (Peprotech, US) for 4 days ...
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bioRxiv - Genetics 2022Quote: ... T-cells were additionally cultured with 30 U/mL IL-2 (Peprotech, 212-12) and 1 μL/mL Mouse T-Activator Dynabeads (Gibco ...
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bioRxiv - Microbiology 2021Quote: ... Glutamine (2 mM) and supplemented with 100 ng/mL human M-CSF cytokine (Peprotech). Three days post-isolation ...