Labshake search
Citations for Peprotech :
751 - 795 of 795 citations for Pregnanediol 3 Glucuronide PDG ELISA Kit 5 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2022Quote: ... Enriched cells were taken into culture in T cell medium (TCM, see Methods – primary human material and cell lines) supplemented with 5 ng/mL IL-7 (Peprotech), 5 ng/mL IL-15 (Peprotech) ...
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bioRxiv - Bioengineering 2022Quote: ... the spheroids were then transferred to spinal cord medium II (ScM II) with 10 nM retinoic acid and 5 ng/mL recombinant human BMP4 (Peprotech) for the next 6 days ...
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bioRxiv - Immunology 2022Quote: ... mice were repeatedly immunized every 14 days (5 times) subcutaneously (s.c.) into the flank with 10μg of human TNFR2 protein (Peprotech, 310-12) and 20μg MPLA (Sigma ...
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bioRxiv - Immunology 2023Quote: ... supplemented with 5% heat-inactivated fetal bovine serum (Biosera FB-1001) and 50ng/ml recombinant M-CSF (Peprotech 300-25). Differentiated macrophages were plated at a density of 1 x 106ml in the appropriate cell culture plate at least 1 d prior to stimulation.
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bioRxiv - Immunology 2023Quote: ... Isolated T cells were otherwise activated using plate bound anti-CD3 (5 μg/ml, inVIVOMAb Cat# BE0002) in complete medium added with 100 U/ml IL-2 (Peprotech) and soluble αCD28 (0.5 μg/ml ...
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bioRxiv - Immunology 2023Quote: ... splenic NK cells were MACs enriched and cultured at 37°C and 5% CO2 in RPMI containing 300U rIL-2 (Peprotech), 50ng/ml rIL-15 (Peprotech ...
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bioRxiv - Neuroscience 2023Quote: ... diluted and seeded on Poly-D-lysine – Matrigel coated coverglasses in a 12 well plate at the concetration of 5×104 cells per well in the Patterning medium [N2B27 medium containing 1ng/mL GDNF (Peprotech), 2ng/ml BDNF (Pepotech) ...
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bioRxiv - Genetics 2023Quote: ... and the cells were washed with phosphate buffered saline (PBS) and cultured in M231 SMDS medium supplemented with 5 ng/mL PDGF-BB (Peprotech) to provoke cell migration ...
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bioRxiv - Immunology 2023Quote: ... Cultures were changed on day 4 to new complete RPMI 1640 medium containing recombinant murine IL-5 (10 ng/ml, Peprotech) and further fed with IL-5 every other day between days 10 to 14 ...
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bioRxiv - Immunology 2024Quote: ... cells were thawed and rested in an 37°C / 5% CO2 incubator in the T cell medium with 50 U/mL rhIL-2 (Peprotech, Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2021Quote: Primary mouse or NMR fibroblasts were seeded at 1 × 104 cells/well onto 24-well plates and stimulated with TNF-α (PeproTech; 50 ng/mL), z-VAD-fmk (Abcam ...
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bioRxiv - Microbiology 2021Quote: ... 2.5×105 HUVECs were plated in wells of 6-well plates and stimulated overnight with 20 ng.ml−1 human recombinant TNFα (PeproTech/Tebu Bio, #300-01A-A) or infected at MOI=200 with Neisseria meningitidis for 30 min ...
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bioRxiv - Genomics 2021Quote: ... Microglia progenitors were harvested from suspension after 14-21 days and plated onto PDL-coated plates in microglia maturation media (2 mM GlutaMax, 1x Antibiotic-Antimycotic, 100 ng/mL IL-34 (Peprotech; Cat. No. 200-34), and 10 ng/mL GM-CSF (Peprotech ...
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bioRxiv - Genomics 2021Quote: ... collected and plated at 10,000 per well in 96-well ultra-low attachment plates in 100 mL embryoid body medium (10 mM ROCK inhibitor, 50 ng/mL BMP-4 (Peprotech; Cat. No. 120-05), 20 ng/mL SCF (Peprotech ...
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bioRxiv - Neuroscience 2022Quote: ... and plated on low-attachment 6-well plate in E8 with 20 ng/ml human basic fibroblast growth factor (bFGF2; Peprotech, Somerset County, NJ, USA) and 20 µM Rho kinase inhibitor (Sigma-Aldrich ...
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bioRxiv - Genetics 2023Quote: ... and transferred to low adherence 10 cm plates in N2B27 medium supplemented with 10 ng/mL murine epidermal growth factor (Peprotech Inc. 315-09-100UG) and 10 ng/mL fibroblast growth factor 2 (Cedarlane Labs CLCYT386) ...
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bioRxiv - Molecular Biology 2019Quote: ... After 7 days cells were detached using TryplE and seeded on non-gelatinized bacterial dishes for suspension culture at 3 million cells per 75cm2 and cultured in N2B27 containing 10ng/mL EGF and FGF (Peprotech 315-09 and 100-18B). After 3 days floating aggregates were seeded on gelatinized dishes ...
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bioRxiv - Cell Biology 2022Quote: ... i3Neurons were cryo-preserved in i3Neuron media (BrainPhys Neuronal Medium supplemented [StemCell] with 2% B27 [Gibco], 10ng/mL BDNF [PreproTech], 10ng/mL NT-3 [PeproTech], and 1µg/mL Laminin [Corning]) with 10% DMSO added ...
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bioRxiv - Genomics 2022Quote: ... Cell pellets were then resuspended (we used EBs from one plate) in differentiation medium containing 10 ng/ml EGF (Peprotech, Cat. No. AF-100-15) and 10 ng/ml Fgf2 (Peprotech ...
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bioRxiv - Molecular Biology 2020Quote: ... NPCs were cultured for up to 5 passages on Matrigel with neural induction media supplemented with 40 ng/ml bFGF (Peprotech: (AF)100-18B) ...
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bioRxiv - Immunology 2021Quote: ... a 6-week-old C57BL/6 mouse was repeatedly immunized 5 times every other week subcutaneously (s.c.) into the flank with 10ug of human TNFR2 protein (Peprotech, 310-12)/20ug MPLA (Sigma ...
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bioRxiv - Cell Biology 2022Quote: ... Activated cells were incubated for 5 days in the presence of the following: IL-12 (25 ng/ml, PeproTech, London, UK) and neutralizing antibody anti–IL-4 (1 μg/ml ...
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bioRxiv - Bioengineering 2023Quote: ... cells were differentiated at a concentration of 5 x 105 cells/mL with 100 nM phorbol 12-myristate 13-acetate (PMA, PeproTech, 1652981) in complete RPMI media for 24 hours followed by 3 days of resting in complete RPMI media ...
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bioRxiv - Immunology 2024Quote: ... A single round of co-sedimentation with retroviral stock was performed in medium containing 5% WEHI-3B-conditioned medium and 10 ng/mL IL-7 (Peprotech, USA). After transduction ...
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bioRxiv - Cancer Biology 2019Quote: ... in 200µl of RPMI supplemented with 2% FBS and 5% Matrigel with or without the addition of human IFN-γ (PeproTech; #300-02), mouse IFN-γ (PeproTech ...
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bioRxiv - Immunology 2021Quote: ... Cells were cultured at 1×106 cells/ml in complete RPMI-1640 supplemented with 20 ng/ml GM-CSF and 5 ng/ml IL-4 (PeproTech, Cranbury, NJ) for 7 days (Replaced 70% of medium on Day 2 and Day 5) ...
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bioRxiv - Neuroscience 2023Quote: ... the embryoid bodies were centrifuged at 200 rcf for 5 min and resuspended in N2B27 supplemented with BDNF (10 µg/ml; PeproTech, 450-02), ascorbic acid ...
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bioRxiv - Pathology 2023Quote: ... or 5 ng/mL of recombinant murine interleukin (IL) −4 and −13 (IL-4, IL-13) (ILs) (PeproTech 214-14, 210-13), were added to the serum-free media ...
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bioRxiv - Cancer Biology 2024Quote: ... according to the manufacturer’s instructions (20 μL of beads per 1 million T-cells) for 5 days and grown in the presence of recombinant interleukin-7 (PeproTech, 200-07) and interleukin-15 (PeproTech ...
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bioRxiv - Cancer Biology 2024Quote: ... according to the manufacturer’s instructions (20 μL of beads per 1 million T-cells) for 5 days and grown in the presence of recombinant interleukin-7 (PeproTech, 200-07) and interleukin-15 (PeproTech ...
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bioRxiv - Biophysics 2023Quote: ... the cells were stimulated at 37 °C for 15 min with 2 mL of 5 nM InlB321 variant (Table S1) or 1 nM HGF (#100-39H, PeproTech, Hamburg, Germany) in serum-free DMEM-F12 ...
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bioRxiv - Neuroscience 2024Quote: ... Acute treatment was evaluated in 9 month-old mice by pre-mixing and co-administering 5 µg HCT-555 with 25 ng recombinant human BDNF (Peprotech, 450-02) into one tibialis anterior muscle and 5 µg HCT-555 with PBS vehicle into the contralateral tibialis anterior ...
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bioRxiv - Cancer Biology 2021Quote: ... or 2) subsequently stimulated with 5 μg/kg T cell chemokine – C-X-C motif chemokine ligand 10 (CXCL10, PeproTech, Rocky Hill, USA) (23) ...
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bioRxiv - Cancer Biology 2023Quote: ... For cytokine treatment cells were stimulated with either 5 ng/mL of TGFβ1 or 50 ng/mL of BMP6 (both from PeproTech, Rocky Hill, NJ, US). Where indicated ...
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bioRxiv - Neuroscience 2020Quote: ... and 1% insulin transferrin selenium (Cat N° 41400045) in IMDM (Cat N° 12440053) supplemented with 5 ng/ml recombinant human basic fibroblast growth factor (bFGF) (Peprotech, Cat N° 100-18B). Medium was changed every 4 days ...
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bioRxiv - Cell Biology 2020Quote: ... They were grown under standard conditions at 37°C and 5% CO2 with NeuroCult basal media (STEMCELL, Vancouver, BC, CA) with EGF (50 ng/ml, PeproTech, Inc., Rocky Hill, NJ, USA), FGF (100 ng/ml ...
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bioRxiv - Immunology 2021Quote: ... Sorted cells were seeded in 10 cm tissue-culture dishes of 80 – 90% confluent OP9 cells containing 5 ng/ml Flt-3L (R&D, Catalogue no.-427-FL) and 1 ng/ml IL-7 (PeproTech, Catalogue no.- 217-17-10). For T-cell development assay ...
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bioRxiv - Developmental Biology 2021Quote: ... the SC solution was centrifuged at 300 g for 5 minutes to remove the supernatant and SFM containing 50 μg/ml stem cell factor (PeproTech, 300-07, Cranbury, NJ, USA), 100 μg/ml IL-3 (PeproTech ...
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bioRxiv - Microbiology 2023Quote: Confluent monolayers of MRC-5 and LEC were treated with 25 ng/mL recombinant human IFN-β and γ (Peprotech #300-02BC and #300-02) in the culture media ...
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bioRxiv - Cancer Biology 2023Quote: ... Activated T-cells were kept in medium supplemented with IL-7 and IL-15 (#200-07 and #200-15, PeproTech, 10 and 5 ng/mL, respectively). Gamma-retroviral transduction was facilitated by RetroNectin (#T100B ...
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bioRxiv - Immunology 2024Quote: ... at 0,5 μg/mL each, with IFN-γ (R&D, cat #285-IF-100) at 1000 IU/mL or with TNF-α (Peprotech, cat #AF- 300-01A) at 1ng/mL ...
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bioRxiv - Cancer Biology 2022Quote: ... Equal amounts of conditioned media were measured using a VEGF-A kit (Peprotech, Rock Hill, NJ) that recognizes human VEGF-A (hVEGF-A ...
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bioRxiv - Cell Biology 2021Quote: ... Each serum was assayed for murine IFN-y in duplicate using an enzyme immunoassay kit (Peprotech, BGK01580).
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bioRxiv - Immunology 2023Quote: ... fixed cells were permeabilised with the Transcription Factor staining kit (eBioscience) for 20min at room temperature and stained for 45min with rabbit anti-mouse RELMα (Peprotech) and biotinylated goat anti-mouse Ym1 (R&D) ...
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bioRxiv - Immunology 2024Quote: ... CD14+ monocytes were isolated from PBMCs using the EasySep Human CD14 Positive Selection Kit (Stem Cell) and cultured with 100U/mL GM-CSF (Peprotech) for 4 days prior to plating for stimulation ...