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Citations for Peprotech :
751 - 800 of 1396 citations for 4 1 tert butoxycarbonyl piperidin 3 yl benzoic acid since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: ... 6 ng/ml murine interleukin 3 (mIL3) and 20 ng/ml murine stem cell factor (mSCF) (all from Peprotech, Rocky Hill, NJ), and incubated at 5% CO2 and 37°C ...
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bioRxiv - Immunology 2024Quote: ... bone marrow precursor cells were obtained from flushed femurs and tibias of C57BL/6J mice and cultured at 3*106 cells/ 10 ml in petri dishes in fully supplemented RPMI and rmGM-CSF (200 U/ml; Peprotech, Hamburg, Germany). Medium was exchanged on days 3 and 6 and the cells were cryopreserved on day 7 for further use.56
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bioRxiv - Neuroscience 2024Quote: ... EBs cell culture media was replaced with X-VIVO 15 media supplemented with 50 ng/ml Flt-3-ligand (PeproTech, 300-19), 50 ng/ml M-CSF (PeproTech ...
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bioRxiv - Cell Biology 2024Quote: ... Monocytes were differentiated into primary human osteoclasts over 10 days, with media refreshed every 2-3 days and receptor activated nuclear factor κB ligand (RANKL, 25 ng/µL (PeproTech, London, UK) added on differentiation day 8 to stimulate osteoclast formation.
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bioRxiv - Bioengineering 2020Quote: ... 10 ng/mL fibroblast growth factor-1 (FGF-1; Peprotech), 5
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bioRxiv - Bioengineering 2020Quote: ... insulin-like growth factor-1 (IGF-1, 20ng/mL; PeproTech) and hydrocortisone (1 μM ...
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bioRxiv - Cell Biology 2023Quote: ... as well as IGF-1 (100 ng mL-1, Peprotech) and FGF-2 (50 ng mL-1 ...
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bioRxiv - Cancer Biology 2023Quote: ... 500 ng mL−1 mR-Spondin-1 (Peprotech 315-32), and 10 mM nicotinamide (Sigma N0636) ...
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bioRxiv - Neuroscience 2024Quote: ... Recombinant human IGF-1 (rhIGF-1) was obtained from PeproTech.
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bioRxiv - Immunology 2021Quote: ... for 4 days at 37°C in complete RPMI medium supplemented with 10 ng/mL recombinant mouse IL-5 (PeproTech) and 20 ng/mL IL-2 (402-ML R&D) ...
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bioRxiv - Immunology 2021Quote: ... for 4 days at 37°C in complete RPMI medium supplemented with 10 ng/mL recombinant mouse IL-5 (PeproTech) and 20 ng/mL IL-2 (402-ML R&D) ...
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bioRxiv - Neuroscience 2020Quote: ... Purified OPCs were expanded in poly-d-lysine coated flasks for 4-5 days in SATO serum-free media containing Platelet-Derived Growth Factor-AA (PDGF-AA, 10 ng/ml, PeproTech), neurotrophin-3 (NT-3 ...
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bioRxiv - Immunology 2021Quote: ... BM cells isolated from BALB/c mice were cultured for 6 days in medium supplemented with granulocyte-macrophage colony-stimulating factor (GM-CSF) and IL-4 (Peprotech) to generate DCs ...
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bioRxiv - Cell Biology 2021Quote: ... 100 U/ml penicillin and 100 µg/ml streptomycin sulfate (Nacalai Tesque) and 4 ng/ml basic fibroblast growth factor (Peprotech).
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bioRxiv - Neuroscience 2022Quote: ... in 100 μl embryoid body medium (10 mM ROCK inhibitor, 50 ng/mL BMP-4 (Peprotech; Cat. No. 120-05), 20 ng/mL SCF (Peprotech ...
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bioRxiv - Cancer Biology 2020Quote: Human liver organoid cultures derived from healthy and HBV infected livers were seeded and cultured for 4 days in EM without Wnt3a-conditioned medium supplemented with 25ng/ml of BMP7 (Peprotech). Hepatic differentiation was induced by culturing human liver organoids in differentiation medium (DM ...
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bioRxiv - Immunology 2021Quote: 750 000 MCs were either FACS-sorted after 24h of coculture with resting or anti-CD3/28 coated beads-activated effector/memory CD4+ T cells or stimulated for 4 hours with 10 ng/mL of IL-33 (Peprotech) or with 100 ng/mL of DNP-BSA (thermo-fischer scientific) ...
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bioRxiv - Immunology 2023Quote: ... were cultured in six-well plates (2 ml per well) in complete RPMI 1640 medium + 10% FBS + 10 ng/ml human IL-4 (PeproTech) + 100 ng/ml human GM-CSF (PeproTech) ...
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bioRxiv - Immunology 2022Quote: Single bone marrow cell suspensions were plated at 4×106 per well in 2 mL of complete IMDM media supplemented with 150 ng/mL of FLT3L (PeproTech). On day 7 of differentiation ...
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bioRxiv - Immunology 2024Quote: ... R&D), IL-27 (20 ng/mL, R&D) as well as with 2 μg/mL anti-mouse IL-4 (Peprotech). Alternatively ...
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bioRxiv - Biochemistry 2023Quote: ... cells were plated (60,000 cells/well in 24-well plates) two days before treatment in complete media supplemented with 25 ng/mL murine IL-4 (Peprotech) for M2-polarization or without any cytokines (M0) ...
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bioRxiv - Immunology 2023Quote: ... Cultures were changed on day 4 to new complete RPMI 1640 medium containing recombinant murine IL-5 (10 ng/ml, Peprotech) and further fed with IL-5 every other day between days 10 to 14 ...
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bioRxiv - Immunology 2022Quote: ... or into bone marrow-derived dendritic cells (BMDC) in cRPMI supplemented with recombinant mouse IL-4 and GM-CSF (Peprotech). Influenza virus strains A/California/04/2009 (H1N1) ...
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bioRxiv - Molecular Biology 2024Quote: ... followed by an additional 4 hours with or without an apoptotic stimulus of 10 ng/ml TNFα (Peprotech, Cranbury, NJ) and 20 μg/mL CHX ...
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bioRxiv - Immunology 2024Quote: ... 100 units/mL penicillin and 100 μg/mL streptomycin] containing IL-4 (20 ng/mL) and GM-CSF (20 ng/ml) (PeproTech) and cultured for 5d with replacement of half of the medium and addition of fresh cytokines every other day ...
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bioRxiv - Neuroscience 2024Quote: ... and IFN-γ (20 ng/mL) (M1 stimulation) or IL-4 (20 ng/mL) and IL-13 (15 ng/mL, all from Peprotech) (M2 stimulation).
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bioRxiv - Cell Biology 2021Quote: ... collected in HBSS+/+ and used as controls (3×105 cells/ml) or were incubated with 10 ng/ml TNFα (PeproTech #AF-300-01A), 10 µg/ml recSema7A Fc (R&D 2068-S7) ...
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bioRxiv - Cancer Biology 2022Quote: ... RRID:CVCL_0161) murine cell lines were grown in RPMI-1640 supplemented with 10% FBS and 10ng/ml murine IL-3 (Peprotech, Cat# 213-13). HEK 293T/17 (ATCC Cat# CRL-11268 ...
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bioRxiv - Microbiology 2024Quote: To differentiate MUTZ-3 cells into a Langerhans cell phenotype (MUTZ-LC) 2×105 cells/ml MUTZ-3 were exposed to 100ng/ml GM-CSF (PeproTech Cat# 300-03), 10ng/ml TGFβ (PeproTech Cat# 100-21 ...
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bioRxiv - Genetics 2021Quote: ... 10μg/L insulin-like growth factor-1 (IGF-1) (Peprotech, USA), and 1% insulin transferrin selenium (ITS ...
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bioRxiv - Cancer Biology 2022Quote: ... Transforming growth factor beta-1 (TGFb-1) was obtained from Peprotech.
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bioRxiv - Immunology 2021Quote: ... Th17 cell cultures received 5 ng/mL TGF-β and 50 ng/mL IL-6 recombinant and 20 µg/mL anti-IL-4 and 100 µg anti-IFN-γ (PeproTech). After 48 h ...
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bioRxiv - Cell Biology 2021Quote: ... purified and cultured with 10% FBS containing GM-CSF (10 ng/mL) and IL 4 (10 ng/mL) (all PeproTech, USA). FBS (10% ...
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bioRxiv - Biophysics 2021Quote: ... after 24 h recombinant human IL-4 (20 ng/ml) and hSCF (100 ng/ml) (both Peprotech, Rocky Hill, NJ, USA) were added ...
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bioRxiv - Microbiology 2021Quote: ... and fresh 10% fetal bovine serum (10% FBS) with GM-CSF (10 ng/mL) and IL-4 (10 ng/mL) (all PeproTech, USA) were added ...
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bioRxiv - Cell Biology 2021Quote: ... Macrophages were then skewed towards the M2 phenotype using recombinant human IL-4 (20 ng/ml) (Peprotech, Cat#AF-200-04), IL-13 (20 ng/ml ...
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bioRxiv - Immunology 2024Quote: ... Transduced cells were incubated for 4 days with plate-bound anti-CD3/anti-CD28 in media supplemented with hIL-2 (0.0344 units/mL, Peprotech #200-02). Transduction efficiency was assessed for each experiment by flow cytometry.
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bioRxiv - Cancer Biology 2022Quote: ... PBMCs were harvested from K3EDTA anti-coagulated whole blood by density gradient centrifugation with Lymphoprep and plated in 12 well plates at 2-4 × 106 cells per well in 10% RPMI + 10 ng/mL human CSF (300-25, Peprotech, UK). Medium was replaced every 2-3 days until adherent populations of macrophages had established ...
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bioRxiv - Cancer Biology 2023Quote: ... with 100 μl of processed CM in the presence or absence of anti-LIF antibody (4 μg/ml) or recombinant mouse LIF (0.1 μg/ml; Peprotech, 250-02). Spheroids were quantified after 8 d culture.
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bioRxiv - Neuroscience 2022Quote: ... then 4 million cells were seeded per well in 2 ml of EB Medium: complete mTeSR + 0.05 ug/ml BMP-4 (PeproTech, #120-05) + 0.05 ug/ml VEGF (PeproTech ...
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bioRxiv - Neuroscience 2022Quote: ... qNSCs were treated with aNSC media with the addition of 0.5 μg/mL of the BMP-4 antagonist noggin (PeproTech 120-10C) to more reproducibly induce quiescence exit.
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bioRxiv - Immunology 2022Quote: ... Whole mouse splenocytes and human PBMCs were plated at a density of 5×106 cells per well in a Cepallet W-type 24 well microplate (DIC, Japan) and cultured for 4 days with or without mouse/human M-CSF (Peprotech, USA). Surviving macrophages adhered to 24-well plates were detached by lowering the temperature on ice ...
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bioRxiv - Immunology 2024Quote: ... was used for sensitizing the cells in the presence of recombinant human IL-4 (Gibco, PeproTech, #200-04; 10 ng/mL) for three days ...
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bioRxiv - Cell Biology 2024Quote: ... centrifuged and resuspended in satellite cell growth medium (SCGM: F-10 Ham’s medium supplemented with 20% FBS, 4 ng/ ml FGF2 (PeproTech; 100-18B), 1% penicillin–streptomycin and 10mM HEPEs) ...
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bioRxiv - Immunology 2022Quote: ... Cells were incubated for 3 hours at 37°C in RPMI media supplemented with or without recombinant mouse IL-21 (20 ng/mL, Peprotech cat. 210-21-100). Cells were stimulated with either anti-Igκ and anti-Igλ or anti-CD40 ...
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bioRxiv - Molecular Biology 2022Quote: ... with cytokines (150 ng/ml SCF, PeproTech, Cat 300-07; 150 ng/ml Flt-3, 20 ng/ml TPO, PeproTech Cat AF-300-18) and 1% HEPES (Sigma-Aldrich ...
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bioRxiv - Cancer Biology 2022Quote: Panc1 and 4039 cells grown to 80% confluency over 3 days were stimulated for 30 minutes with 30 ng/mL PDGF (Peprotech Cat. no. 100-00AB), then placed immediately on ice ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Immature iPSC-SNs were maintained in N2 medium supplemented with human growth factors (25 ng/mL NGF-β, 450-01; BDNF, 450-02; GDNF, 450-10; NT-3, 450-03; Peprotech, Cranbury, NJ, USA), and 0.2 mM L-ascorbic acid (A4403 ...
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bioRxiv - Cancer Biology 2023Quote: ... 10 ng/mL IL-3 (Cat#200-03), and 10 ng/mL granulocyte colony-stimulating factor (G-CSF, Cat#300-23) (all from Peprotech, Rock Hill, NJ, USA). Additionally ...
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bioRxiv - Cell Biology 2024Quote: ... containing growth factors (50 ng ml−1 EGF, PeproTech; 500 ng ml−1 R-spondin, PeproTech; and 100 ng ml−1 Noggin, PeproTech) were added and refreshed every 2 or 3 days ...