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Citations for Peprotech :
651 - 700 of 762 citations for Synaptic Ras GTPase Activating Protein 1 Syngap1 Antibody since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2024Quote: ... Ex vivo expansion of B cells was performed using recombinant murine IL-4 (100 u ml-1, Peprotech) and anti-mouse CD40 antibody (5 µg ml-1 ...
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bioRxiv - Neuroscience 2024Quote: ... microglia proliferation was increased by treatment with 100 ng/ml murine Colony-stimulating factor-1 (M-CSF1) (Peprotech) for 48 hours.
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bioRxiv - Cancer Biology 2021Quote: ... coated plates in serum reduced media (1% HS) supplemented with 50 ng/mL of murine β-NGF (Peprotech, Israel).
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Variation in Leishmania chemokine suppression driven by diversification of the GP63 virulence factorbioRxiv - Microbiology 2021Quote: ... normalized live parasites (1×106) or heterologously expressed GP63 was incubated with 500pg/ml of human recombinant CXCL10 (Peprotech) for the indicated time at 37°C ...
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bioRxiv - Neuroscience 2020Quote: ... OPCs were passaged and plated onto poly-d-lysine coated glass coverslips in 24 well plates at 50,000 cells per well in SATO serum-free media containing NT-3 (1 ng/ml, PeproTech), and CNTF (10 ng/ml ...
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bioRxiv - Developmental Biology 2022Quote: ... undifferentiated WT ESCs were seeded at a density of 250.000 cells per well in 6-well plates and treated with 200ng/mL Wnt antagonist Dickkopf 1 (DKK1) (Peprotech) or 10μM iCRT3 [inhibitor of β-catenin–responsive transcription] (Sigma ...
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bioRxiv - Neuroscience 2022Quote: ... and the media was changed to serum-free DMEM/F12 with 1 % neomycin and 25 ng/ml BDNF (Peprotech). The cells were used from day 12 to day 18 after differentiation.
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bioRxiv - Immunology 2021Quote: ... cultured at 1–2 Mio/ml in 12-well plates in the presence of 50 ng/ml IL4 (PeproTech) and 100 ng/ml GM-CSF (Immunotools ...
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bioRxiv - Immunology 2020Quote: ... cultured at 1–2 Mio/ml in 12-well plates in the presence of 50 ng/ml IL4 (PeproTech) and 100 ng/ml GM-CSF (Immunotools ...
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bioRxiv - Immunology 2021Quote: ... at a bead to cell ratio of 3:1 and 1,000 U/mL of recombinant human IL-2 (Peprotech). Fresh IL-2 containing media was added every 48 h and Human T-activator CD3/CD28 Dynabeads were added again at day 7 and removed at day 15 of expansion ...
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bioRxiv - Immunology 2020Quote: ... Primary splenic B cell stimulation was performed with αCD40 (1 μg/ml, eBioscience) plus IL4 (20 ng/ml, PeproTech) or with LPS (25 ng/ml ...
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bioRxiv - Neuroscience 2024Quote: ... Culture medium was replaced by a 1:2 mixture of Bottenstein–Sato (BS) medium with PDGF-A (0.5%, Peprotech) and NCM ...
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bioRxiv - Immunology 2024Quote: ... stem cell factor (SCF; 5% supernatant of murine SCF-secreting CHO transfectants) and IL-4 (1 ng/ml, PeproTech) were added to the medium (89) ...
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bioRxiv - Cancer Biology 2024Quote: ... Growth factors were also given to media surrounding basement membrane extract (Cultrex, Biotechne): 1% Mouse recombinant Noggin (Peprotech, UK), 1% mouse recombinant EGF (Invitrogen ...
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bioRxiv - Molecular Biology 2023Quote: ... and resuspended in differentiation medium I-10 + M-CSF (i.e., IMDM, 10 % FBS, 1 mg/ml Pen/Strep containing 25 ng/ml recombinant murine M-CSF (PeproTech)) at a density of 1-2 Mio ...
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bioRxiv - Neuroscience 2023Quote: ... and 100 ng/mL of CX3CL1 (Fractalkine/ chemokine (C-X3-C motif) ligand 1) (Peprotech, Catalog No. 300-31) for up to 12 days.
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bioRxiv - Microbiology 2023Quote: ... and 1 µg/mL soluble anti-CD28 (clone CD28.2, eBioscience) in the presence of 50 IU/mL IL-2 (PeproTech) for three days ...
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bioRxiv - Bioengineering 2022Quote: ... 20% R-spondin 1 conditioned medium,50% Wnt3a conditioned medium and 10 μM Y-27632 dihydrochloride (PeproTech Cranbury, USA). Culture medium was replenished every two days.
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bioRxiv - Immunology 2022Quote: ... heterologous Human naive T cells from healthy volunteers were sorted as CD3+CD4+CD45RA+CD45RO- and added to the culture at a ratio of 1:10 (DC:T cell) in the presence of IL-2 recombinant cytokine (50 ng/ml) (Peprotech). Five days post co-culture ...
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bioRxiv - Neuroscience 2023Quote: ... and plated at 50,000 cells/cm2 using iN D4 media (NBM media + 1:50 B27 + BDNF, GDNF, CNTF (10 ng/ml, Peprotech). Doxycycline (2ug/ml ...
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bioRxiv - Immunology 2024Quote: ... and 1% penicillin/streptomycin antibiotics and differentiated in the presence of 20ng/mL GM-CSF (Peprotech cat: 300-03) and 10ng/mL M-CSF (Peprotech cat ...
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bioRxiv - Molecular Biology 2024Quote: ... Primocin was removed and Noggin-conditioned media was replaced with recombinant Noggin (100 ng ml−1, 250-38, Peprotech). A day before the electroporation ...
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bioRxiv - Neuroscience 2024Quote: ... The NB-B27-S included human ciliary neurotrophic factor (CNTF, 10 ng/mL, 1:1000, Peprotech, AF-450-13), glial-derived neurotrophic factor (GDNF ...
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bioRxiv - Immunology 2024Quote: ... activated OT-1 T cells were initially expanded in IL-2 (20ng/ml) and IL-12 (20ng/ml; Peprotech) before being switched into IL-2 alone ...
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bioRxiv - Neuroscience 2024Quote: ... and plated at 50,000 cells/cm2 using iN D4 media (NBM media + 1:50 B27 + BDNF, GDNF, CNTF (10 ng/ml, Peprotech). Doxycycline (2ug/ml ...
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bioRxiv - Bioengineering 2024Quote: ... and 1% Penicillin-Streptomycin (Themo Fisher, CAT: 15070063) and 25 ng/ml of M-CSF (Peprotech, CAT: 315-02). Media was changed after 3 days ...
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bioRxiv - Cell Biology 2024Quote: ... was induced by 1-3 days of 2.5 µM CHIR99021 (Stemgent) and 3-5 days of 100 ng/ml Activin A (Peprotech) in RPMI medium ...
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bioRxiv - Cancer Biology 2021Quote: ... SNKO-1 cells were obtained from DSMZ and cultured in RPMI with 10% FSB and 10 ng/ml GM-CSF (PeproTech). EOL-1 cells were obtained from DSMZ and cultured in RPMI with 10% FBS ...
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bioRxiv - Immunology 2021Quote: ... and cultured at 1-2 × 106/ml in 12-well plates with 1 ml of differentiation medium containing 50 ng/ml interleukin 4 (IL-4, 200–04 A, Peprotech) and 100 ng/ml granulocyte-monocyte colony stimulating factor (GM-CSF ...
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bioRxiv - Neuroscience 2021Quote: ... once daily for 14 days starting at P30 with either vehicle (saline) or rhIGF-1 (2.5 mg/kg; Peprotech, USA) dissolved in saline with 0.01% Bovine Serum Albumin (weight/volume).
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bioRxiv - Molecular Biology 2020Quote: ... supplemented with 1% HSA (Baxter) and cytokines (SCF 100ng/mL, FLT3L 100ng/mL and TPO 100ng/mL; all from PeproTech)55 ...
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bioRxiv - Neuroscience 2021Quote: ... 1% penicillin/streptomycin) was changed every 3-5 days and M-CSF was supplemented (Peprotech 315-02, 5 ng/mL) for 2 weeks ...
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bioRxiv - Neuroscience 2020Quote: ... The final pellets were dissociated with pre-warmed 10% fetal bovine serum in DMEM supplemented with 20 ng mL-1 mouse M-CSF (PeproTech) and plated in culture dishes ...
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bioRxiv - Bioengineering 2021Quote: ... T cells were cultured at a density of 1 million cells/mL in ImmunoCult-XF T cell Expansion Medium (STEMCELL) supplemented with 200 U/mL IL-2 (Peprotech) and stimulated with ImmunoCult Human CD3/CD28/CD2 T cell Activator (STEMCELL ...
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bioRxiv - Cancer Biology 2021Quote: ... Cells were allowed to attach on coverslip for 1 h and then cells were incubated with murine recombinant CCL2 (Peprotech) for 5 h ...
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bioRxiv - Bioengineering 2021Quote: Lyophilized 90 % PEGDMA microrods were loaded by resuspending ∼100,000 (100 k) microrod aliquots in 20 μL of 1 mg/mL recombinant human β-NGF (Peprotech) as described in the Protein loading of PEGDMA microrods section ...
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bioRxiv - Microbiology 2020Quote: ... The cells were cultured in RPMI 1640 (Nissui) supplemented with 10% heat-inactivated calf serum (Biowest) and 1 ng/mL recombinant human interleukin 6 (IL-6, PeproTech). HeLa and 293T cells were cultured in DMEM (Nacalai tesque ...
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bioRxiv - Bioengineering 2021Quote: ... the organoids were transferred from the aforementioned PDMS microwells to a previously published spinning bioreactor19 with a speed of 80 revolutions per minute in E6 medium containing 10 ng mL−1 FGF2 (Peprotech), 20 ng mL−1 BDNF (Peprotech) ...
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bioRxiv - Neuroscience 2020Quote: ... Monocyte chemoattractant protein 1/chemokine C-C motif ligand 2 (MCP1/CCL-2) levels were evaluated using a commercially available ELISA kit (PeproTech) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: Splenic T-cells from Ldlr-/- and T-AbcdkoLdlr-/- mice were isolated and stimulated with Mouse T-Activator CD3/CD28 Dynabeads (ratio beads to cells 1:2) in the presence of 100 U/mL IL-2 (Peprotech) and 5 ng/mL TGFβ1 (Peprotech ...
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bioRxiv - Immunology 2020Quote: ... and were then cultured at 1-2 x 106/ml in 12-well plates with differentiation media containing 50 ng/ml Interleukin 4 (Peprotech) and 100 ng/ml granulocyte-monocyte colony stimulating factor (GM-CSF ...
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bioRxiv - Immunology 2021Quote: The differentiation of isolated monocytes into macrophages was performed in RPMI 1640 GlutaMAx medium with 10% FCS and 1% P/S containing 100 ng/mL macrophage colony-stimulating factor (M-CSF) (PeproTech) for 7 days ...
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bioRxiv - Immunology 2021Quote: ... Activated C7 cells were concentrated and 4 ml of culture was resuspended in 1 ml RPMI+ containing 10 μg/mL ESAT61-20 peptide + 20 ng/mL IL-12 (Peprotech) + 20 ng/mL IL-2 (Peprotech) ...
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The skin environment controls local dendritic cell differentiation and function through innate IL-13bioRxiv - Immunology 2021Quote: ... were harvested and one million cells were resuspended in 1 mL of TCM in 24 wells plates and stimulated with recombinant mouse IL-13 (Peprotech) at a concentration of 100 ng/mL for 30 min at 37°C ...
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bioRxiv - Immunology 2020Quote: ... the obtained bone marrow cells were cultured in RPMI-1640 containing 10% FBS and 10 ng mL−1 mouse macrophage colony-stimulating factor (M-CSF, Peprotech) in a humidified 5% CO2 atmosphere at 37 °C for six days ...
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bioRxiv - Cell Biology 2021Quote: ... The fused cells were dispersed in 80 ml of GIT medium (Wako, Japan) supplemented with 1 ng/ml IL-6 (PeproTech) and 1×HAT (Kohjin-Bio) ...
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bioRxiv - Cell Biology 2023Quote: ... cells were either given fresh MC medium or cultured in basal MC medium (1% FBS) or in basal MC medium supplemented with 50ng/mL recombinant human VEGF-A165 (100-20; Peprotech) and culture for another 3 days.
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bioRxiv - Cancer Biology 2024Quote: ... at 3:1 bead: T-cell ratio together with human recombinant IL-15 and IL-7 (PeproTech; 5 ng/mL) in AIM-V medium supplemented with 5% FBS ...
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bioRxiv - Immunology 2024Quote: ... Cells were expanded for at least 1 week in the presence of IL-3 and 30 ng/mL recombinant SCF (Peprotech) to obtain peritoneal cavity-derived mast cells (PCMCs) ...
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bioRxiv - Developmental Biology 2024Quote: ... cells were replated as single cells onto poly-D-lysine-laminin-coated plates at 1×106 cells/cm2 for astrogenic switch and progenitor expansion in N2B27 media supplemented with 10 ng/mL FGF2 (Peprotech) and 10 ng/mL Human EGF (Peprotech ...