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Citations for Peprotech :
601 - 650 of 1168 citations for SARS CoV 2 Spike Glycoprotein S1 His tag Insect Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... Switzerland) for 11 days in the human plasma serum-containing medium with 3000 IU/ml of IL-2 (PeproTech). The cell cultures were supplemented with fresh media and IL-2 every 2–4 days ...
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bioRxiv - Immunology 2021Quote: ... (2) treated with recombinant mouse IL-12 (5 ng/ml) and IL-18 (25 ng/ml, both from Peprotech), (3 ...
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bioRxiv - Genetics 2022Quote: ... clone 145-2C11, WEHI antibody facility), anti-CD28 (2μg/ml, clone 3751, WEHI antibody facility) and human IL-2 (Peprotech). CD8 T cell cultures contained 25μg/ml anti-mouse IL2 (clone S4B6 ...
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bioRxiv - Neuroscience 2023Quote: ... the medium was replaced to DMEM/F12 plus 2% B27 with HB-EGF (10 ng/ml, Peprotech, 100-47), EGF (20 ng/ml ...
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bioRxiv - Immunology 2023Quote: ... in the presence of 2mg/mlaCD28 (clone 37.51, WEHI antibody facility) and 100U/ml recombinant human IL-2 (Peprotech), in 96 well flat-bottom plates at 104 cells per well ...
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bioRxiv - Microbiology 2023Quote: ... and 1 µg/mL soluble anti-CD28 (clone CD28.2, eBioscience) in the presence of 50 IU/mL IL-2 (PeproTech) for three days ...
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bioRxiv - Neuroscience 2024Quote: ... Culture medium was replaced by a 1:2 mixture of Bottenstein–Sato (BS) medium with PDGF-A (0.5%, Peprotech) and NCM ...
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bioRxiv - Cancer Biology 2023Quote: ... in the presence of OVA323–339 peptide (20 μg/ml, Caslo ApS) and IL-2 (5,000 U/ml, Peprotech). As positive control ...
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bioRxiv - Immunology 2024Quote: CD8 T cells from WT p14 or Irf5-/- p14 mice were isolated as described above and cultured with or without 1µg/ml gp33 peptide in complete RPMI medium supplemented with 2ng/ml recombinant human IL-2 (Peprotech). Cells were then collected at 6 or 24 hours after incubation and washed with PBS ...
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bioRxiv - Immunology 2023Quote: ... seeded at 2 ml per well in 12 well flat-bottom plates and treated with C5a (PeproTech, 300-70) or avacopan for 72 hrs ...
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bioRxiv - Developmental Biology 2021Quote: ... FACS-isolated Flk1+ mesodermal cells were cultured at 5 x104 cells/cm2 in SP34 plus SCF (Peprotech) and VEGF (R&D) ...
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bioRxiv - Immunology 2022Quote: ... and cells were plated at 5 × 105 cells/10 ml with 20 ng/ml M-CSF (PeproTech). On day 6 BMDM were replated at 1 × 106 cells per well in 24-well plates and stimulated with 10 µg/ml Imiquimod (Biolegend ...
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bioRxiv - Bioengineering 2019Quote: ... The cells were differentiated into dendritic cells using mouse GM-CSF (20 ng/mL; Peprotech, Rocky Hill, NJ). Nanoparticle formulations were administered to the cells for 24 hours ...
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bioRxiv - Immunology 2021Quote: ... with 2% FBS (HyClone Thermo Fischer Scientific) for 15 minutes with or without murine IL-7 (50 ng/mL, PeproTech). Following stimuli ...
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bioRxiv - Microbiology 2020Quote: ... the PBMCs were cultured with the synthetic peptide mixture of SARS-CoV-2 at a concentration of 1μg/ml or DMSO in RPMI 1640 medium containing 10% FCS and 20 U/ml recombinant human IL-2 (Peprotech) for 7 days ...
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bioRxiv - Immunology 2021Quote: ... cells were removed from the TCR signal and re-cultured by doubling the area and volume in media supplemented with 20 U/ml IL-2 (Peprotech), which was added every 3 days ...
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bioRxiv - Microbiology 2019Quote: ... and maintained as previously described[2] in media supplemented with 10ng/ml recombinant interleukin-4 and 10ng/ml granulocyte-macrophage colony stimulating factor (Peprotech). Bone marrow macrophages (BMMΦ ...
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bioRxiv - Bioengineering 2020Quote: ... the iPSCs were first cultured in basal medium (RPMI-1640 with 2% B27) supplemented with 100 ng/ml Activin A (Peprotech) and 3 μM CHIR99021 for 3 days and then with 5 ng/ml bFGF ...
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bioRxiv - Cell Biology 2021Quote: ... and the medium was replaced with complete NS media supplemented with 20ng/ml FGF-2 and 50ng/ml BMP4 (PeproTech) (quiescence medium) ...
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bioRxiv - Cell Biology 2021Quote: ... reporter mice or HES1mScarlet-I/mScarlet-I mice and cultured in complete NS media as previously described (Pollard, 2013) supplemented with 10ng/ml FGF-2 (PeproTech), 10ng/ml EGF2 (PeproTech ...
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bioRxiv - Molecular Biology 2022Quote: ... The isolated cells were cultured in RPMI medium supplemented with 20% (v/v) FBS and 100 ng/mL human recombinant IL-2 (Peprotech). Two days prior to transduction ...
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bioRxiv - Immunology 2022Quote: ... 0.25 µg/mL Amphotericin B and 2 mM L-glutamine and treated with 10 ng/mL recombinant IL-12p70 (Peprotech) for 24 hours for supernatant IFN-γ analysis.
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bioRxiv - Immunology 2022Quote: ... for Th1 polarization the medium was supplemented with 5 ng/ml IL-2 and 10 ng/ml IL-12 (both from Peprotech), and 10 μg/ml anti-IL-4 (11B11 ...
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bioRxiv - Immunology 2022Quote: ... cells were maintained in 24-well G-Rex plates (Wilson Wolf) in NK MACS media (Miltenyi Biotech) supplemented with human IL-2 (100 IU/mL, Peprotech) and human IL-15 (20 ng/mL ...
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bioRxiv - Bioengineering 2021Quote: ... were isolated from blood of a healthy donors by density-gradient centrifugation and grown in RPMI 1640 medium supplemented with 10% FCS and 1000 U/ml recombinant IL-2 (PeproTech) and activated with 1 μg/ml anti-CD3 and anti-CD28 antibodies ...
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bioRxiv - Immunology 2022Quote: ... cells were transferred to 24-well plates and incubated for three days in medium containing 10 U/mL rhIL-2 (Peprotech) to allow establishment of the knockout ...
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bioRxiv - Immunology 2022Quote: ... After a one hour resting period cells were transferred to 24-well plates and incubated for three days in medium containing 10U/mL recombinant human IL-2 (Peprotech) to allow establishment of the knockout ...
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bioRxiv - Immunology 2019Quote: Human CD14+ monocytes were purified from leukocyte preparations purchased from the New York Blood Center and differentiated into blood-derived macrophages for 1–2 d in 10 ng/ml M-CSF (PeproTech) and RPMI 1640 medium (Life Technologies)/10% defined FBS (Hyclone) ...
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bioRxiv - Immunology 2021Quote: ... 1-5 × 105 PBMCs were stimulated with 1 μg/ml of S864-882 for 10 days and recombinant human IL-2 (1 ng/ml, Peprotech) was added at day 4 and day 7 ...
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bioRxiv - Immunology 2020Quote: ... and were then cultured at 1-2 x 106/ml in 12-well plates with differentiation media containing 50 ng/ml Interleukin 4 (Peprotech) and 100 ng/ml granulocyte-monocyte colony stimulating factor (GM-CSF ...
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bioRxiv - Immunology 2019Quote: ... 50μM 2-ME and 100ng/ml recombinant murine macrophage colony stimulating factor (M-CSF, PeproTech Inc., Rocky Hill, NJ, USA) for 6 days ...
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bioRxiv - Cancer Biology 2019Quote: ... and maintained in culture for a total of approximately 2-3 months in DMEM supplemented with 1 ng/mL IL-3 (Peprotech). Mycoplasma testing was not performed ...
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bioRxiv - Bioengineering 2019Quote: ... Media was supplemented with VEGF (50 ng/mL) on day 2 and with VEGF (50 ng/mL) and angiopoietin-1 (ANG-1) (100 ng/mL) (Peprotech) on days 3 ...
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bioRxiv - Cell Biology 2020Quote: ... After 2 days of culture with 20 ng/ml M-CSF and 20 ng/ml recombinant human RANKL (PeproTech, UK), the wells were rinsed twice with PBS and incubated with 10% bleach solution for 30 min at room temperature ...
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Neutralizing activity of broadly neutralizing anti-HIV-1 antibodies against primary African isolatesbioRxiv - Immunology 2020Quote: ... A total of 2×106 CD4+ T lymphocytes were activated using anti CD3/CD2/CD28 beads (Miltenyi) and cultured in the presence of 100 U/mL IL-2 (Peprotech) at 37 °C and 5% CO2 ...
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bioRxiv - Immunology 2020Quote: ... Clonal MEL5 CD8+ T cells were maintained in R10 supplemented with 200 IU/ml IL-2 and 25 ng/ml IL-15 (both from PeproTech). The MEL5 TCR is specific for the heteroclitic HLA-A*0201-restricted Melan-A epitope ELAGIGILTV26–35/A27L [17 ...
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bioRxiv - Bioengineering 2022Quote: ... cells were incubated with StemPro-34 medium from days 2 to 5 with a cocktail of growth factors: vascular endothelial growth factor (VEGF; PeproTech), BMP-4 ...
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The logic of native enhancer-promoter compatibility and cell-type-specific gene expression variationbioRxiv - Genomics 2022Quote: ... in N2B27 supplemented with 20ng/ml Activin A and 12ng/ml Fibroblast growth factor 2 (FGF2) (MPI-CBG protein facility, or Peprotech) for 14-15 passages ...
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bioRxiv - Immunology 2022Quote: Single bone marrow cell suspensions were plated at 4×106 per well in 2 mL of complete IMDM media supplemented with 150 ng/mL of FLT3L (PeproTech). On day 7 of differentiation ...
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bioRxiv - Immunology 2023Quote: ... Isolated T cells were otherwise activated using plate bound anti-CD3 (5 μg/ml, inVIVOMAb Cat# BE0002) in complete medium added with 100 U/ml IL-2 (Peprotech) and soluble αCD28 (0.5 μg/ml ...
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bioRxiv - Bioengineering 2023Quote: ... After gelation samples were submerged in IMR90 medium with/without 2 ng/mL TGF-β2 (100-35B; PeproTech, Cranbury, NJ). Cultures were monitored for loss of collagen adhesion and contraction of the gel for 21 d and survival was quantified as described above.
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bioRxiv - Immunology 2023Quote: ... Cells were resuspended in complete RMPI (RPMI supplemented with 10% v/v FBS, 100 penicillin, 100 streptomycin) with 50U of IL-2 (PeproTech) and CD3/CD28 Human T-activator Dynabeads (Thermo Fisher ...
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bioRxiv - Immunology 2023Quote: ... were cultured in six-well plates (2 ml per well) in complete RPMI 1640 medium + 10% FBS + 10 ng/ml human IL-4 (PeproTech) + 100 ng/ml human GM-CSF (PeproTech) ...
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bioRxiv - Immunology 2023Quote: ... T cells were re-plated with fresh medium containing 10 ng/ml rhIL-2 or a combination of 10 ng/ml IL-7 and IL-15 (all Peprotech) and differentiated at a density of 5×105 cells/ml for additional 4 days ...
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bioRxiv - Neuroscience 2023Quote: ... Then NPCs were seeded on Matrigel®-coated flasks at 50 000 cells/cm2 and kept in Astrocyte Induction medium (DMEM/F-12 supplemented with 1x N2 supplement, 1x B27 supplement, 10ng/ml FGF-2 (PeproTech) and 10ng/ml EGF (Miltenyi) ...
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bioRxiv - Immunology 2023Quote: ... cells were washed and then expanded and differentiated in complete IMDM (as above) containing 20 ng/mL recombinant human IL-2 (Peprotech) for a further 4 days ...
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bioRxiv - Neuroscience 2024Quote: ... the medium was changed to NDM supplemented with 20 ng/ml fibroblast growth factor-2 (FGF2; Peprotech, Westlake Village, CA). Media was changed daily ...
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bioRxiv - Bioengineering 2024Quote: ... CD8 T cells were transferred to a new 96-well round bottom plate to remove activation and continued to be cultured in complete RPMI supplemented with a higher concentration of IL-2 (10ng/ml; Peprotech).
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bioRxiv - Immunology 2024Quote: ... R&D), IL-27 (20 ng/mL, R&D) as well as with 2 μg/mL anti-mouse IL-4 (Peprotech). Alternatively ...
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bioRxiv - Cancer Biology 2024Quote: ... according to manufacturer’s specifications in the presence of 100 IU/mL recombinant human (rh) IL-2 and 10 ng/mL rhIL-7 (Peprotech). Spinoculation with neat 293 Galv9 retroviral supernatant was performed at 48 ...