Labshake search
Citations for Peprotech :
601 - 650 of 852 citations for M CSF Mouse HEK293 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Immunology 2023Quote: ... bone marrow was taken from the tibias and femurs of 9-week-old C57BL/6 Lgals9 -/- mice or wild type litter mates and cultured in RPMI containing 10 % FBS and 3 % granulocyte macrophage colony-stimulating factor (mGM-CSF) (Peprotech). for 7 days ...
-
bioRxiv - Cancer Biology 2023Quote: ... and conditioned media containing approximately 100 ng/ml SCF for the generation of neutrophil-biased cells or GM-CSF 10 ng/ml (Peprotech) for the generation of macrophage progenitors ...
-
bioRxiv - Immunology 2023Quote: ... Bone marrow-derived dendritic cells (BMDCs) were generated in tissue culture using recombinant GM-CSF (Peprotech, Cat. AF-315-03). Class B CpG 1826 oligonucleotide was synthesized by Integrated DNA Technologies ...
-
bioRxiv - Cancer Biology 2023Quote: ... Differentiation of BM cells into DCs was carried out in low attachment 10 mm cell culture dish in presence of bone marrow-differentiation media in presence of recombinant murine Granulocyte-Macrophage Colony-Stimulating Factor (GM-CSF) (Cat. 315-03, Peprotech) for 48 a ...
-
bioRxiv - Immunology 2024Quote: ... 3×105 CD14+ monocytes were seeded in a 12-well plate in 1 mL complete medium containing 20 ng/mL GM-CSF (#300-03; Peprotech) or 50 ng/mL M-CSF (#300-25 ...
-
bioRxiv - Immunology 2024Quote: ... filtered and cultured for 7 days in complete RPMI medium (RPMI-1640 medium containing L-glutamine plus 10% fetal calf serum) containing 20ng/mL recombinant GM-CSF (Peprotech).
-
bioRxiv - Developmental Biology 2020Quote: ... treated with 5 ng/ml recombinant (r) human TGFβ1 derived from HEK293 cells (100-21, PeproTech, Rocky Hill, NJ, USA) for 1 ...
-
bioRxiv - Cell Biology 2021Quote: ... purified and cultured with 10% FBS containing GM-CSF (10 ng/mL) and IL 4 (10 ng/mL) (all PeproTech, USA). FBS (10% ...
-
bioRxiv - Immunology 2022Quote: ... X-Vivo 15 media was supplemented with 100 ng/ml human granulocyte-macrophage colony-stimulating factor (GM-CSF) and 20 ng/ml human interleukin 4 (IL-4) (both cytokines obtained from Peprotech, Germany), and cells were cultured with 5% CO2 ...
-
bioRxiv - Immunology 2019Quote: ... 1ml of cell suspension was plated per well in tissue culture-treated 24-well plates and supplemented with 20ng/ml recombinant GM-CSF and 5ng/ml TGFβ (Peprotech, USA). Cells were cultured at 37°C ...
-
bioRxiv - Microbiology 2021Quote: ... and fresh 10% fetal bovine serum (10% FBS) with GM-CSF (10 ng/mL) and IL-4 (10 ng/mL) (all PeproTech, USA) were added ...
-
bioRxiv - Immunology 2019Quote: ... bone marrow cells were plated at a density of 5×105 cells/mL in RPMI-1640 + 10% FCS + 100 U/mL penicillin/streptomycin + 1 mM β-mercaptoethanol (BM medium) with 20 ng/mL murine GM-CSF (Peprotech) and cultures in a humidified environment at 37°C and 5% CO2 ...
-
bioRxiv - Immunology 2021Quote: ... then the remaining negatively sorted cells were resuspended at 5×105 cells/ml in complete Iscove’s modified Dulbecco’ s medium supplemented with Granulocyte-macrophage colony-stimulating factor (GM-CSF) (PeproTech, #315-03), FLT-3L (PeproTech ...
-
bioRxiv - Cancer Biology 2022Quote: ... PBMCs were harvested from K3EDTA anti-coagulated whole blood by density gradient centrifugation with Lymphoprep and plated in 12 well plates at 2-4 × 106 cells per well in 10% RPMI + 10 ng/mL human CSF (300-25, Peprotech, UK). Medium was replaced every 2-3 days until adherent populations of macrophages had established ...
-
bioRxiv - Developmental Biology 2021Quote: ... and treated with 25 ng/ml recombinant (r) human TGFβ1 derived from HEK293 cells (100-21, PeproTech, Rocky Hill, NJ, USA) for 24 hours.
-
bioRxiv - Biochemistry 2021Quote: Recombinant human platelet-derived growth factor-BB (PDGF-BB) and recombinant human transforming Growth Factor-β1 (TGFβ1; HEK293 derived) were purchased from PeproTech (Hamburg, Germany) and they were dissolved in sterile ddH2O ...
-
bioRxiv - Cell Biology 2021Quote: ... bone marrow progenitor cells were collected in cRPMI and replated in cRPMI containing 20 ng/mL recombinant murine GM-CSF (Peprotech, 315-03) (DC media) ...
-
bioRxiv - Cell Biology 2021Quote: ... For dendritic cell differentiation the bone marrow was resuspended in 25 ml of complete RPMI 1640 media supplemented with 20 ng/ml GM-CSF (Peprotech #315-03), 10 ng/ml IL-4 (Peprotech #AF-214-14 ...
-
bioRxiv - Cancer Biology 2022Quote: ... the selected monocytes were cultured in complete Advanced-RMPI 1640 supplemented with either 20 ng/mL human GM-CSF (PeproTech, RockyHill, NJ), for M1 differentiation ...
-
bioRxiv - Immunology 2019Quote: ... 50μM 2-ME and 10 ng/ml of recombinant murine granulocyte– macrophage colonystimulating factor (GM-CSF, PeproTech Inc., Rocky Hill, NJ, USA) for 7 days ...
-
bioRxiv - Immunology 2021Quote: ... Cells were cultured at 1×106 cells/ml in complete RPMI-1640 supplemented with 20 ng/ml GM-CSF and 5 ng/ml IL-4 (PeproTech, Cranbury, NJ) for 7 days (Replaced 70% of medium on Day 2 and Day 5) ...
-
bioRxiv - Immunology 2023Quote: ... A total of 1.5×106/well CD14+ monocytes were seeded in a 12-well plate with 50 ng/mL of hM-CSF (Peprotech, 300-25). On day 6 ...
-
bioRxiv - Immunology 2023Quote: ... 100 U/mL penicillin and 100 μg/mL streptomycin) to which 20 ng/mL of recombinant GM-CSF (#315-03, PeproTech, Hamburg, Germany) was added ...
-
bioRxiv - Microbiology 2023Quote: ... and 50 μM β-mercaptoethanol and differentiated with 20 ng/ml murine granulocyte-macrophage colony-stimulating factor (GM-CSF) (Peprotech, 315-03). BMDCs were stained for CD11c and analyzed by flow cytometry (LSR-Fortessa flow cytometer ...
-
bioRxiv - Immunology 2023Quote: ... in the presence of 40 ng/ml of granulocyte-macrophage colony-stimulating factor (GM-CSF) and IL-4 (both from Peprotech or Biolegend). Cells were cultured in 24-well plates in DMEM (Gibco) ...
-
bioRxiv - Cell Biology 2024Quote: ... and 20 ng/mL Oncostatin M (OSM; 300-10, PeproTech, USA). The liver organoids were isolated from Matrigel by scraping and pipetting and passaged on day 16 ...
-
bioRxiv - Bioengineering 2024Quote: ... 20 ng/mL recombinant human oncostatin M (OSM; Peprotech; 300-10), and 100 nM dexamethasone (DEX ...
-
bioRxiv - Immunology 2021Quote: ... BM cells were plated at 2 x 105 in 6-well plates and cultured for 6 days in IMDM + 10% fetal bovine serum (Wisent) supplemented with 20 ng/mL of GM-CSF (Peprotech, Rocky Hill, NJ). BMDMs were washed twice with D-PBS (Wisent ...
-
bioRxiv - Immunology 2020Quote: ... 1640 Medium supplemented with 10% fetal bovine serum (35-076-CV; Coring, New York, USA) and 20 ng/μL Recombinant Human GM-CSF (300-03; Peprotech, Rocky Hill, USA).BEAS-2B cells (CRL-9609 ...
-
bioRxiv - Immunology 2020Quote: ... 2 million bone marrow cells were co-cultured with 1 × 105 GL261 cells in 6-well plates in 2 ml RPMI/10% FBS supplemented with 40 ng/ml GM-CSF and 80 ng/ml IL-13 (PeproTech, Rocky Hill, NJ) for 3-4 days ...
-
bioRxiv - Molecular Biology 2022Quote: CD34+GFP+ HSPCs from UCB were cultured in granulocytic differentiation medium (SCF 25ng/mL, PeproTech, Cat 300-07; GM-CSF 10ng/mL, PeproTech, Cat 300-03) for 14 days ...
-
bioRxiv - Immunology 2022Quote: ... Cells were differentiated to macrophages in RPMI x1640 supplemented with 10% FBS containing 10 ng/ml of recombinant human GM-CSF (Peprotech, New Jersey, US) for 7 days ...
-
bioRxiv - Immunology 2022Quote: ... bone marrow was isolated from tibiae and femurs and single-cell suspensions were cultured at 10*106 cells per 100 mm bacteriological Falcon petri dish in 10 ml complete T cell medium supplemented with 20 ng/ml GM-CSF (PeproTech, Rocky Hill, NJ). At day 3 ...
-
bioRxiv - Microbiology 2024Quote: To differentiate MUTZ-3 cells into a Langerhans cell phenotype (MUTZ-LC) 2×105 cells/ml MUTZ-3 were exposed to 100ng/ml GM-CSF (PeproTech Cat# 300-03), 10ng/ml TGFβ (PeproTech Cat# 100-21 ...
-
bioRxiv - Immunology 2024Quote: Purified monocytes were plated at a concentration of 1 million cells/mL in 96 U-bottom wells and cultured in RPMI 1640 medium supplemented with 10% fetal bovine serum and 1% penicillin/streptomycin with 10 μg/mL of GM-CSF (Peprotech, cat #300-03). Cells were then cultured for 16-20h with or without stimulation.
-
bioRxiv - Immunology 2024Quote: ... and cultured in DC media (15% FBS, 1% A/A, 50µM α-MTG, 20ng/mL granulocyte-macrophage colony stimulating factor (GM-CSF; Peprotech cat#: 315-03)
-
bioRxiv - Immunology 2024Quote: ... 100 μg/ml streptomycin and 50 μM β-mercaptoethanol plus 20 ng/ml murine granulocyte-macrophage colony-stimulating factor (GM-CSF) (Peprotech, 315-03). On day 4 ...
-
bioRxiv - Immunology 2020Quote: ... Cells were then plated into Petri dishes and cultured in BMDC differentiation medium (RPMI, 10% FBS, 1% penicillin-streptomycin, 1x sodium pyruvate, 1x β-mercaptethanol) supplemented with GM-CSF (Peprotech, Rocky Hill, NJ). Media was refreshed on day 2 ...
-
bioRxiv - Cancer Biology 2023Quote: ... 10 ng/mL IL-3 (Cat#200-03), and 10 ng/mL granulocyte colony-stimulating factor (G-CSF, Cat#300-23) (all from Peprotech, Rock Hill, NJ, USA). Additionally ...
-
bioRxiv - Immunology 2022Quote: ... CD14+ cells were incubated for 6 days in the presence of 50 ng/ml granulocyte-macrophage colony- stimulating factor (GM-CSF) and 20 ng/ml interleukin-4 (IL-4) (PeproTech®, Neuilly sur Seine, France). The medium used for culture was RPMI ...
-
bioRxiv - Immunology 2021Quote: ... #AV-7016-1) and recombinant murine IFNγ (20 ng/m(PEPROTECH, #315-05)) or towards an alternative activation phenotype (M(Il-4) ...
-
bioRxiv - Cancer Biology 2022Quote: ... All cytokines for murine macrophage polarization (mIL-4, mIL-13, mIFN-γ, hIFN-γ, hTGF-β, hIL-2, and mGM-CSF) were purchased from PeproTech (Rocky Hill, NJ). KMO inhibitor (UPF648) ...
-
Microbial signals and lymphotoxin drive TNF-independent death of A20 and ABIN-1 deficient epitheliumbioRxiv - Immunology 2021Quote: ... Recombinant mouse IL-1β and mouse LIGHT were purchased from Peprotech. Pam3CSK4 ...
-
bioRxiv - Cell Biology 2019Quote: ... mouse IL-6 and mouse SCF (10 ng/mL each) (Peprotech) in a 6-well culture plate ...
-
bioRxiv - Bioengineering 2022Quote: ... 20 ng/ml HGF and 10 ng/ml oncostatin M (Peprotech, catalogue no. 300-10), 1% (v/v ...
-
bioRxiv - Immunology 2022Quote: ... mouse IL-2 (Peprotech), and human TGF-beta (Peprotech) ...
-
bioRxiv - Developmental Biology 2022Quote: ... TSC-m medium consist of TI medium with addition of 50ng/ml EGF (Peprotech, AF-100-15), 2μM CHIR99021 (Axon Medchem 1386 ...
-
bioRxiv - Immunology 2021Quote: ... Mouse recombinant IL-21 (PeproTech) was added to co-cultures at 10ng/ml ...
-
bioRxiv - Immunology 2021Quote: ... recombinant mouse IL-12 (Peprotech) and anti-IL-4 (BioXCell) ...
-
bioRxiv - Cell Biology 2021Quote: ... 10ng/mL mouse SCF (Peprotech) and 0.1% PVA (Sigma ...