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Citations for Peprotech :
551 - 600 of 790 citations for G CSF Mouse CHO since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... Macrophage precursors were added to the NPC culture in 1:1 neural maintenance medium:microglia medium ((DMEM-F12+Glutamax, 100 ng/mL M-CSF (Peprotech, 300-25), 100 ng/mL IL-34 (Peprotech ...
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bioRxiv - Cell Biology 2022Quote: ... The cell suspension was washed with PBS and plated in DMEM with 10 ng mL-1 M-CSF (Peprotech Inc, 315-02). Following 5 days of culture ...
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bioRxiv - Systems Biology 2020Quote: ... 2 mM glutamine and 10% fetal calf serum in the presence of 50 ng/ml of M-CSF (Peprotech, cat# 300-25). 5 × 105 CD14+CD16− monocytes or × 105 resting macrophages were activated with 10 ng/ml of lipopolysaccharides (LPS ...
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bioRxiv - Cell Biology 2020Quote: ... cells were plated in 10 cm diameter Petri dishes with 10 ng/mL macrophage colony-stimulating factor (M-CSF, PeproTech, London, UK) for 24 h ...
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bioRxiv - Cell Biology 2021Quote: ... bone marrow progenitor cells were collected in cRPMI and replated in cRPMI containing 20 ng/mL recombinant murine GM-CSF (Peprotech, 315-03) (DC media) ...
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bioRxiv - Cancer Biology 2021Quote: ... 10% FBS) in the presence of macrophage colony stimulating factor (M-CSF) (30 ng/mL, #315-02, Peprotech, Rocky Hill, NJ, USA). After three days in culture ...
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bioRxiv - Cancer Biology 2021Quote: ... BMCs were cultured in DMEM/F-12 supplemented with 20 ng/mL murine M-CSF (315-02-10, Peprotech, Cranbury, NJ, USA) in a density of 4 × 106/mL ...
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bioRxiv - Cell Biology 2021Quote: ... For dendritic cell differentiation the bone marrow was resuspended in 25 ml of complete RPMI 1640 media supplemented with 20 ng/ml GM-CSF (Peprotech #315-03), 10 ng/ml IL-4 (Peprotech #AF-214-14 ...
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bioRxiv - Cancer Biology 2022Quote: ... the selected monocytes were cultured in complete Advanced-RMPI 1640 supplemented with either 20 ng/mL human GM-CSF (PeproTech, RockyHill, NJ), for M1 differentiation ...
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bioRxiv - Immunology 2019Quote: ... 50μM 2-ME and 10 ng/ml of recombinant murine granulocyte– macrophage colonystimulating factor (GM-CSF, PeproTech Inc., Rocky Hill, NJ, USA) for 7 days ...
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bioRxiv - Immunology 2021Quote: ... Cells were cultured at 1×106 cells/ml in complete RPMI-1640 supplemented with 20 ng/ml GM-CSF and 5 ng/ml IL-4 (PeproTech, Cranbury, NJ) for 7 days (Replaced 70% of medium on Day 2 and Day 5) ...
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bioRxiv - Bioengineering 2022Quote: ... macrophage colony stimulating factor (M-CSF) and receptor activator of nuclear factor kappa-B ligand (RANKL) were from PeproTech (London, United Kingdom). Bombyx mori L ...
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bioRxiv - Immunology 2023Quote: ... 100 U/mL penicillin and 100 μg/mL streptomycin) to which 20 ng/mL of recombinant GM-CSF (#315-03, PeproTech, Hamburg, Germany) was added ...
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bioRxiv - Microbiology 2023Quote: ... and 50 μM β-mercaptoethanol and differentiated with 20 ng/ml murine granulocyte-macrophage colony-stimulating factor (GM-CSF) (Peprotech, 315-03). BMDCs were stained for CD11c and analyzed by flow cytometry (LSR-Fortessa flow cytometer ...
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bioRxiv - Immunology 2023Quote: ... Bone marrow was plated into non-cell-culture treated 10cm petri dishes in 8mL supplemented RPMI medium containing either 20ng/mL recombinant M-CSF (Peprotech, #315-02) or 20ng/mL recombinant GM-CSF (Peprotech ...
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bioRxiv - Immunology 2023Quote: ... in the presence of 40 ng/ml of granulocyte-macrophage colony-stimulating factor (GM-CSF) and IL-4 (both from Peprotech or Biolegend). Cells were cultured in 24-well plates in DMEM (Gibco) ...
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bioRxiv - Immunology 2023Quote: ... A total of 1.5×106/well CD14+ monocytes were seeded in a 12-well plate with 50 ng/mL of hM-CSF (Peprotech, 300-25). On day 6 ...
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bioRxiv - Cell Biology 2023Quote: ... BMMs were seeded at a density of 1.2 × 105 cells/well into 24-well culture plates and incubated in α-MEM (SH30265.01; Hyclone, Rockford, IL, USA) containing 20 ng/mL macrophage colony-stimulating factor (M-CSF) (300-25; PeproTech, Cranbury, NJ, USA). To induce osteoclast differentiation ...
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bioRxiv - Immunology 2019Quote: ... 1.5□g of recombinant IL-2 (Peprotech) and 7.5□g of functional grade anti-IL-2 antibody (JES6-1 ...
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bioRxiv - Immunology 2021Quote: ... BM cells were plated at 2 x 105 in 6-well plates and cultured for 6 days in IMDM + 10% fetal bovine serum (Wisent) supplemented with 20 ng/mL of GM-CSF (Peprotech, Rocky Hill, NJ). BMDMs were washed twice with D-PBS (Wisent ...
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bioRxiv - Microbiology 2021Quote: ... bone marrow cells were cultured in RPMI-1640 medium supplemented with 10% fetal bovine serum (FBS) in the presence of M-CSF (10 ng/ml, PeproTech, Cat# 315-02) for 7-9 days.
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bioRxiv - Cancer Biology 2022Quote: ... Cells were then cultured on untreated plates for 7 days adding 10 ng/ml recombinant murine M-CSF (Peprotech, AF-315-02-100) at day of isolation ...
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bioRxiv - Immunology 2020Quote: ... 1640 Medium supplemented with 10% fetal bovine serum (35-076-CV; Coring, New York, USA) and 20 ng/μL Recombinant Human GM-CSF (300-03; Peprotech, Rocky Hill, USA).BEAS-2B cells (CRL-9609 ...
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bioRxiv - Immunology 2020Quote: ... 2 million bone marrow cells were co-cultured with 1 × 105 GL261 cells in 6-well plates in 2 ml RPMI/10% FBS supplemented with 40 ng/ml GM-CSF and 80 ng/ml IL-13 (PeproTech, Rocky Hill, NJ) for 3-4 days ...
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bioRxiv - Molecular Biology 2022Quote: CD34+GFP+ HSPCs from UCB were cultured in granulocytic differentiation medium (SCF 25ng/mL, PeproTech, Cat 300-07; GM-CSF 10ng/mL, PeproTech, Cat 300-03) for 14 days ...
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bioRxiv - Immunology 2019Quote: ... the cells were resuspended and cultured in macrophage-conditioned medium containing complete 1640 medium containing 10% (vol/vol) FBS and 100 U/ml penicillin and streptomycin and 20 ng/ml murine M-CSF (Peprotech, cat. # 315-02) for 7 days.
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bioRxiv - Cell Biology 2021Quote: ... For macrophage differentiation the bone marrow was resuspended in 25 ml of complete RPMI 1640 media supplemented with 20 ng/ml M-CSF (Peprotech #AF-315-02) and 50 ng/ml Amphotericin B ...
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bioRxiv - Molecular Biology 2021Quote: ... BMDM and AM were cultured in Dulbeccòs Modified Eagle’s Medium (DMEM) + 10% heat-inactivated fetal bovine serum (FBS) supplemented with 20 ng/ml recombinant murine M-CSF (PeproTech, Rocky Hill, NJ) and 20 ng/ml recombinant murine RANKL (PeproTech) ...
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bioRxiv - Immunology 2021Quote: ... Human monocyte-derived macrophages (HMDMs) were differentiated from purified CD14+ monocytes in the presence of recombinant human M-CSF (50 ng/ml; PeproTech, Cranbury, NJ, USA) for 6 days in RPMI 1640 medium supplemented with 10% FBS ...
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bioRxiv - Immunology 2020Quote: ... Isolated PBMC were washed with PBS and resuspended by RPMI-1640 which supplemented 50ng/ml recombinant human macrophage colony-stimulating factor (M-CSF) (AF-300-25-500, PeproTech, Cranbury, NJ, USA), then cultured at 37°C in 5% CO2 for 5 days to differentiate into human monocyte derived macrophages (hMDMs ...
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bioRxiv - Immunology 2022Quote: ... Cells were differentiated to macrophages in RPMI x1640 supplemented with 10% FBS containing 10 ng/ml of recombinant human GM-CSF (Peprotech, New Jersey, US) for 7 days ...
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bioRxiv - Immunology 2022Quote: ... bone marrow was isolated from tibiae and femurs and single-cell suspensions were cultured at 10*106 cells per 100 mm bacteriological Falcon petri dish in 10 ml complete T cell medium supplemented with 20 ng/ml GM-CSF (PeproTech, Rocky Hill, NJ). At day 3 ...
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bioRxiv - Immunology 2022Quote: ... or CD11b+ enriched single cell suspensions of B16F10 tumors were cultured in the presence of 50 ng/mL recombinant M-CSF (Peprotech, Cranbury, NJ, USA) in RPMI (Wellgene ...
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bioRxiv - Immunology 2023Quote: ... were differentiated from PBMCs by culturing the cells in RPMI complete medium supplemented with 10% AB-positive human serum and 15 ng/ml M-CSF (PeproTech, 300-25-100UG). After 2 days of culturing ...
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bioRxiv - Microbiology 2024Quote: To differentiate MUTZ-3 cells into a Langerhans cell phenotype (MUTZ-LC) 2×105 cells/ml MUTZ-3 were exposed to 100ng/ml GM-CSF (PeproTech Cat# 300-03), 10ng/ml TGFβ (PeproTech Cat# 100-21 ...
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bioRxiv - Immunology 2024Quote: Purified monocytes were plated at a concentration of 1 million cells/mL in 96 U-bottom wells and cultured in RPMI 1640 medium supplemented with 10% fetal bovine serum and 1% penicillin/streptomycin with 10 μg/mL of GM-CSF (Peprotech, cat #300-03). Cells were then cultured for 16-20h with or without stimulation.
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bioRxiv - Immunology 2024Quote: ... and cultured in DC media (15% FBS, 1% A/A, 50µM α-MTG, 20ng/mL granulocyte-macrophage colony stimulating factor (GM-CSF; Peprotech cat#: 315-03)
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bioRxiv - Microbiology 2021Quote: ... and 20ng/ml recombinant murine interferon-g (Peprotech) (IFNg ...
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bioRxiv - Immunology 2020Quote: ... Cells were then plated into Petri dishes and cultured in BMDC differentiation medium (RPMI, 10% FBS, 1% penicillin-streptomycin, 1x sodium pyruvate, 1x β-mercaptethanol) supplemented with GM-CSF (Peprotech, Rocky Hill, NJ). Media was refreshed on day 2 ...
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bioRxiv - Immunology 2022Quote: ... CD14+ cells were incubated for 6 days in the presence of 50 ng/ml granulocyte-macrophage colony- stimulating factor (GM-CSF) and 20 ng/ml interleukin-4 (IL-4) (PeproTech®, Neuilly sur Seine, France). The medium used for culture was RPMI ...
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bioRxiv - Cell Biology 2020Quote: ... and resuspended in macrophage complete medium (DMEM/F12, 10% FBS, 1% penicillin/streptomycin, 100 U/ml recombinant murine M-CSF; Peprotech, Rocky Kill, NJ; Cat# 315-02). 5 ml of macrophage complete medium was added on day 3 ...
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bioRxiv - Bioengineering 2020Quote: ... and resuspended in macrophage complete medium (DMEM/F12, 10% FBS, 1% pen/strep, 100 U/ml recombinant murine M-CSF; Peprotech, Rocky Kill, NJ; Cat# 315-02). 5 ml of macrophage complete medium was added on day 3 ...
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bioRxiv - Cancer Biology 2022Quote: ... All cytokines for murine macrophage polarization (mIL-4, mIL-13, mIFN-γ, hIFN-γ, hTGF-β, hIL-2, and mGM-CSF) were purchased from PeproTech (Rocky Hill, NJ). KMO inhibitor (UPF648) ...
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bioRxiv - Immunology 2023Quote: ... followed by gentle removal of media and replacement with 20mL/dish of complete medium (IMDM, 10% hiFBS, 5mM PenStrep antibiotic) supplemented with 50ng/mL M-CSF (PeproTech, Rockyhill NJ, USA, Cat#: 300-25). On Days 2 and 5 ...
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Microbial signals and lymphotoxin drive TNF-independent death of A20 and ABIN-1 deficient epitheliumbioRxiv - Immunology 2021Quote: ... Recombinant mouse IL-1β and mouse LIGHT were purchased from Peprotech. Pam3CSK4 ...
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bioRxiv - Cell Biology 2019Quote: ... mouse IL-6 and mouse SCF (10 ng/mL each) (Peprotech) in a 6-well culture plate ...
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bioRxiv - Immunology 2022Quote: ... mouse IL-2 (Peprotech), and human TGF-beta (Peprotech) ...
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bioRxiv - Immunology 2021Quote: ... Mouse recombinant IL-21 (PeproTech) was added to co-cultures at 10ng/ml ...
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bioRxiv - Immunology 2021Quote: ... recombinant mouse IL-12 (Peprotech) and anti-IL-4 (BioXCell) ...
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bioRxiv - Cell Biology 2021Quote: ... 10ng/mL mouse SCF (Peprotech) and 0.1% PVA (Sigma ...