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Citations for Peprotech :
451 - 492 of 492 citations for Dengue Virus Serotype 3 DIII envelope protein since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: ... from serial transplants were thawed and maintained in medium (DMEM with 15% FBS + 100 units/ml penicillin streptomycin and 2mM L-glutamine) and supplemented with 10ng/ml IL-3 (Peprotech, 213-13), 10ng/ml IL-6 (Peprotech 216-1 ...
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bioRxiv - Cancer Biology 2020Quote: ... 6 ng/ml murine Interleukin 3 (mIL3) and 20 ng/ml murine stem cell factor (mSCF) (all from Peprotech, Rocky Hill, NJ), and incubated in 5% CO2 at 37°C ...
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bioRxiv - Immunology 2022Quote: ... Cells were transdifferentiation for 7 days at 60,000 cells/cm2 in medium containing 50 ng/ml IL-3 (200-03 B, Peprotech, New Jersey, USA), 50 ng/ml M-CSF (300-25 B ...
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bioRxiv - Cancer Biology 2020Quote: Tumor cells were seeded in 24-well-plates at a density of 3×105 cells per well and treated either with a serial dilution series of IFNγ (PeproTech, 300-02) (starting at 50 ng/ml in two-fold dilution steps ...
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bioRxiv - Cell Biology 2020Quote: ... were maintained in medium (RPMI with 20% FBS + 100 units/ml penicillin streptomycin and 2mM L-glutamine) and supplemented with 10ng/ml IL-3 (Peprotech, 213-13). For in vivo experiments ...
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bioRxiv - Cancer Biology 2022Quote: ... cells were starved for 3 h prior to ligand stimulation with EGF (50 ng/mL, 10 min; PeproTech, Rocky Hill, NJ, USA), and were lysed in ice-cold lysis buffer (50 mM Tris-HCl ...
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bioRxiv - Immunology 2024Quote: ... cells were isolated from bone marrow of C57Bl/6 mice and cultured with granulocyte-macrophage stimulating factor (100ng or 2×103 units per 3×106 cells, Peprotech, Cranbury, NJ) in RPMI-1640 media containing 10% heat inactivated fetal bovine serum ...
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bioRxiv - Bioengineering 2024Quote: ... Isolated PB-NK cells were expanded with irradiated K562-mb15-41BBL feeder cells (1-3 days before NK cell isolation, 100 Gy) along with 50 IU/mL IL-2 (Peprotech, Cranbury, NJ) and 2 ng/mL IL-15 (Peprotech ...
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bioRxiv - Cell Biology 2022Quote: ... After 24 hours cells were re-plated at 30,000 cells/ml in gelatinized 24-well plates in DMEM complete medium supplemented with IL-3 (Peprotech, Cat#213-13) mCSF-1 (Peprotech ...
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bioRxiv - Cancer Biology 2023Quote: ... 6 ng/ml murine interleukin 3 (mIL3) and 20 ng/ml murine stem cell factor (mSCF) (all from Peprotech, Rocky Hill, NJ), and incubated at 5% CO2 and 37°C ...
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bioRxiv - Immunology 2024Quote: ... bone marrow precursor cells were obtained from flushed femurs and tibias of C57BL/6J mice and cultured at 3*106 cells/ 10 ml in petri dishes in fully supplemented RPMI and rmGM-CSF (200 U/ml; Peprotech, Hamburg, Germany). Medium was exchanged on days 3 and 6 and the cells were cryopreserved on day 7 for further use.56
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bioRxiv - Neuroscience 2024Quote: ... EBs cell culture media was replaced with X-VIVO 15 media supplemented with 50 ng/ml Flt-3-ligand (PeproTech, 300-19), 50 ng/ml M-CSF (PeproTech ...
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bioRxiv - Neuroscience 2024Quote: ... OPCs from each animal were expanded in 3x 175cm2 flasks for 3-4 days in the presence of 10ng/mL platelet derived growth factor-aa (PDGFAA, Peprotech 100-13A). Cells were harvested fresh for each round of experiments and used at the time of first passage ...
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bioRxiv - Cell Biology 2024Quote: ... Monocytes were differentiated into primary human osteoclasts over 10 days, with media refreshed every 2-3 days and receptor activated nuclear factor κB ligand (RANKL, 25 ng/µL (PeproTech, London, UK) added on differentiation day 8 to stimulate osteoclast formation.
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bioRxiv - Neuroscience 2022Quote: ... IL-34 (100 ng/mL) and GM-CSF (10 ng/mL) (all protein growth factors were from Peprotech). Prior to thaw ...
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bioRxiv - Bioengineering 2023Quote: ... then for the next 4 days media was supplemented with 50ng/mL bone morphogenetic protein 4 (BMP4) (Peprotech), and for the final 4 days 50ng/mL of hepatocyte growth factor (HGF ...
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bioRxiv - Cell Biology 2021Quote: ... collected in HBSS+/+ and used as controls (3×105 cells/ml) or were incubated with 10 ng/ml TNFα (PeproTech #AF-300-01A), 10 µg/ml recSema7A Fc (R&D 2068-S7) ...
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bioRxiv - Cancer Biology 2022Quote: ... RRID:CVCL_0161) murine cell lines were grown in RPMI-1640 supplemented with 10% FBS and 10ng/ml murine IL-3 (Peprotech, Cat# 213-13). HEK 293T/17 (ATCC Cat# CRL-11268 ...
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bioRxiv - Microbiology 2024Quote: To differentiate MUTZ-3 cells into a Langerhans cell phenotype (MUTZ-LC) 2×105 cells/ml MUTZ-3 were exposed to 100ng/ml GM-CSF (PeproTech Cat# 300-03), 10ng/ml TGFβ (PeproTech Cat# 100-21 ...
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bioRxiv - Immunology 2024Quote: ... at a 1:3 T cell:bead ratio cultured in R10 media and 20 IU of recombinant human IL-2 (Peprotech, Cat-no. 200-02). Cells were transduced with CAR lentivirus at a multiplicity of infection (MOI ...
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bioRxiv - Immunology 2022Quote: ... Cells were incubated for 3 hours at 37°C in RPMI media supplemented with or without recombinant mouse IL-21 (20 ng/mL, Peprotech cat. 210-21-100). Cells were stimulated with either anti-Igκ and anti-Igλ or anti-CD40 ...
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bioRxiv - Molecular Biology 2022Quote: ... with cytokines (150 ng/ml SCF, PeproTech, Cat 300-07; 150 ng/ml Flt-3, 20 ng/ml TPO, PeproTech Cat AF-300-18) and 1% HEPES (Sigma-Aldrich ...
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bioRxiv - Cancer Biology 2022Quote: Panc1 and 4039 cells grown to 80% confluency over 3 days were stimulated for 30 minutes with 30 ng/mL PDGF (Peprotech Cat. no. 100-00AB), then placed immediately on ice ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Immature iPSC-SNs were maintained in N2 medium supplemented with human growth factors (25 ng/mL NGF-β, 450-01; BDNF, 450-02; GDNF, 450-10; NT-3, 450-03; Peprotech, Cranbury, NJ, USA), and 0.2 mM L-ascorbic acid (A4403 ...
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bioRxiv - Cancer Biology 2023Quote: ... 10 ng/mL IL-3 (Cat#200-03), and 10 ng/mL granulocyte colony-stimulating factor (G-CSF, Cat#300-23) (all from Peprotech, Rock Hill, NJ, USA). Additionally ...
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bioRxiv - Neuroscience 2021Quote: ... Cells were treated with either vehicle (equal volume added of 0.1% BSA in H2O) or 100nM BDNF recombinant protein (Peprotech, #450-02 ...
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bioRxiv - Immunology 2020Quote: ... Strips were washed with TBS-T (TBS, 0.1% Tween-20) and bound protein was detected with specific rabbit anti-chemokine antibodies (Peprotech) followed by an HRP-conjugated anti-rabbit antibody (Abcam ...
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bioRxiv - Cell Biology 2022Quote: ... i3Neurons were cryo-preserved in i3Neuron media (BrainPhys Neuronal Medium supplemented [StemCell] with 2% B27 [Gibco], 10ng/mL BDNF [PreproTech], 10ng/mL NT-3 [PeproTech], and 1µg/mL Laminin [Corning]) with 10% DMSO added ...
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bioRxiv - Developmental Biology 2024Quote: ... the medium was changed to late hCNCCs medium by supplementing the early hCNCCs medium with 3 µM CHIR 99021 (Selleckchem, catalog no. S2924) and 50 pg/mL BMP2 (Peprotech, catalog no. AF-120-02) to enhance cell proliferation and reduce migration28 ...
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bioRxiv - Molecular Biology 2024Quote: ... cells were detached using TrypLE and seeded on nongelatinized bacterial dishes for suspension culture at 3 million cells per 75 cm2 and cultured in N2B27 containing 10 ng/mL EGF and FGF (Peprotech 315-09 and 100-18B). After 3 days ...
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bioRxiv - Neuroscience 2020Quote: Treatment of primary neurons - The primary neurons were treated with APOE from conditioned media or recombinant APOE protein (350-02, 350-04, Peprotech). For conditioned media treatment ...
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bioRxiv - Immunology 2022Quote: ... mice were repeatedly immunized every 14 days (5 times) subcutaneously (s.c.) into the flank with 10μg of human TNFR2 protein (Peprotech, 310-12) and 20μg MPLA (Sigma ...
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The logic of native enhancer-promoter compatibility and cell-type-specific gene expression variationbioRxiv - Genomics 2022Quote: ... in N2B27 supplemented with 20ng/ml Activin A and 12ng/ml Fibroblast growth factor 2 (FGF2) (MPI-CBG protein facility, or Peprotech) for 14-15 passages ...
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bioRxiv - Immunology 2021Quote: ... a 6-week-old C57BL/6 mouse was repeatedly immunized 5 times every other week subcutaneously (s.c.) into the flank with 10ug of human TNFR2 protein (Peprotech, 310-12)/20ug MPLA (Sigma ...
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bioRxiv - Immunology 2024Quote: ... China) gradient and pulsed with 0.5 µM rUreB protein in the presence of 5 U/ml rmIL-2 (PeproTech, NJ, USA). Cells were cultured in “RF-10” medium consisting of RPMI 1640 (Gibco ...
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bioRxiv - Neuroscience 2021Quote: ... Cells were dissociated using 5mM EDTA in HBSS and resuspended in media with B-27 supplement for 4 hours before incubation with recombinant BDNF protein (100nM, Peprotech, #450-02) or vehicle (equal volume of 0.1% BSA in H2O) ...
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bioRxiv - Systems Biology 2024Quote: ... Osteogenic differentiation was initiated on Day 0 by culturing in osteogenic differentiation medium consisting of growth medium and 100 ng/mL bone morphogenetic protein-4 (BMP4) (PeproTech, 315-27). The osteogenic differentiation medium was replaced every second day.
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bioRxiv - Developmental Biology 2024Quote: ... a cocktail of small molecules and recombinant proteins were used with the following final concentration: 100 ng/mL BMP4 (PeproTech, 120-05ET), 100 ng/mL FGF10 (PeproTech ...
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bioRxiv - Bioengineering 2021Quote: ... supplemented with 50 ng/mL FGF2 and 30 ng/mL animal-free recombinant human Bone Morphogenic Protein 4 (BMP4, Peprotech, AF-120-05ET) for 24 hours ...
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bioRxiv - Neuroscience 2021Quote: ... yolk sac embryoid bodies (YS-EBs) were generated by treating EBs with bone morphogenetic protein 4 (BMP4, 50 ng/ml, Peprotech; to induce mesoderm), stem cell factor (SCF ...
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bioRxiv - Biochemistry 2023Quote: ... 40,000 cells were seeded per cavity of a 96-well plate and incubated for 20 h with serial dilutions (1000 – 0.076 pM) of the fusion protein Af2-IL-2 and the reference recombinant human IL-2 (PeproTech, Rocky Hill NJ, USA), respectively ...
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bioRxiv - Neuroscience 2024Quote: ... were generated from hiPSCs and induced to yolk sac EBs (YS-EBs) by adding bone morphogenetic protein 4 (BMP4, 50 ng/mL, Peprotech; to induce mesoderm), vascular endothelial growth factor (VEGF ...