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Citations for Click Chemistry Tools :
1 - 50 of 73 citations for Mouse Fragile X Mental Retardation 1 Neighbor Protein FMR1NB ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2020Quote: ... Enrichment of alkyne-modified proteins was conducted using the Click-&-Go Protein Enrichment Kit (Click Chemistry Tools) and the protocols thereof ...
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bioRxiv - Microbiology 2023Quote: ... using the Click-&-Go protein enrichment kit (Click Chemistry Tools) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2020Quote: ... to AHA containing proteins was carried out using Click-&-Go™ Protein Reaction Buffer Kit (Click Chemistry Tools) according to the manufacturer’s instructions ...
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bioRxiv - Physiology 2023Quote: ... Enriched proteins were eluted for mass spectrometry analysis according to the Click-&-GoTM Dde Protein Enrichment Kit (Click Chemistry Tools, 1153).
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bioRxiv - Neuroscience 2020Quote: ... Alkyne-conjugated biotin and Protein Reaction Buffer kit were from Click Chemistry Tools.
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bioRxiv - Cell Biology 2021Quote: ... was conducted using the Click-&-Go Protein Reaction Buffer Kit (Click Chemistry Tools 1262) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2020Quote: ... The Click-iT reaction was performed with the Click-&-Go Protein Reaction Buffer Kit (Click Chemistry Tools) and AF488 alkyne (Click Chemistry Tools ...
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bioRxiv - Microbiology 2022Quote: ... L-AHA-containing proteins were labeled with Cy7 by strain-promoted azide-alkyne click-chemistry employing 1 μM dibenzylcyclooctyne-Cy7 (DBCO-Cy7) (Click Chemistry Tools, USA, Scottsdale). After methanol-chloroform extraction ...
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bioRxiv - Biochemistry 2022Quote: ... Fluorophores for protein labeling were from Click Chemistry Tools Inc.
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bioRxiv - Neuroscience 2022Quote: ... adherent cells or tissue sections were permeabilized for 20 minutes in 0.5% Triton X-100 and incubated in the dark for 30 minutes with copper-dependent click reaction mixture (Click Chemistry Tools #1350) to label EdU with Azide-biotin (Click Chemistry Tools #1265 ...
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bioRxiv - Neuroscience 2022Quote: ... Cells were permeabilized for 20 minutes in 0.5% Triton X-100 and incubated in the dark for 30 minutes with copper-dependent click reaction mixture (Click Chemistry Tools #1350) to label EdU directly with fluorophores (Azide-488 or –647) ...
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bioRxiv - Biochemistry 2021Quote: Protein pellets functionalized with the TAMRA-Azide-Desthiobiotin (Click Chemistry Tools, Scottsdale, AZ) were precipitated by methanol/chloroform as described above and resuspended in 500 μL of 6M Urea + 25 mM ammonium bicarbonate ...
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bioRxiv - Biophysics 2024Quote: ... All fluorophores used to generate fluorescently labeled proteins were purchased from Click Chemistry Tools. Cy3-labeled ...
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bioRxiv - Immunology 2023Quote: ... beads (now containing bound protein) were washed and incubated with 5 μM DBCO-488 (Click Chemistry Tools) for 30 min at RT or overnight at 4°C ...
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Modifications in the T arm of tRNA globally determine tRNA maturation, function and cellular fitnessbioRxiv - Molecular Biology 2023Quote: ... Cell opening and fluorescent labeling of L-AHA-containing proteins with DBCO-PEG4-Carboxyrhodamine 110 (Click Chemistry Tools) were performed as described 29 ...
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bioRxiv - Synthetic Biology 2023Quote: ... and 44 µL of a 1:1 mixture of BTTAA (Click Chemistry Tools, 77.4 mg mL-1 PBS) and copper sulfate pentahydrate (7.4 mg mL-1 water) ...
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bioRxiv - Cell Biology 2024Quote: ... Click-labeling of nascent proteins was performed with 8.3 µM dibenzocyclooctyne (DBCO)−488 (Click Chemistry Tools Catalog #1278-5) in PBS for 30 minutes at 4°C ...
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bioRxiv - Microbiology 2023Quote: ... and then resuspended to OD600 of 0.6 in 1 mL PBS + 1 μM AZDye 488 DBCO (Click chemistry tools, cat# 1278-1). Cells were covered with aluminum foil and rocked for 1 hour ...
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bioRxiv - Plant Biology 2023Quote: ... 44.5 μL of labelled proteins were incubated with 2 μM Cy5 Picolyl Azide (Click Chemistry Tools, 50 μM stock in DMSO) or Fluorescein Picolyl Azide (Click Chemistry Tools ...
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bioRxiv - Plant Biology 2024Quote: ... 44.5 μl of labelled proteins were treated with 2 μM Cy5 Picolyl Azide (Click Chemistry Tools, 50 μM stock in DMSO) or Fluorescein Picolyl Azide (Click Chemistry Tools ...
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bioRxiv - Bioengineering 2022Quote: ... and Click-&-Go Cell Reaction Buffer Kit (Click Chemistry Tools), and 3 ...
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bioRxiv - Biochemistry 2020Quote: ... Lysates containing 50 μg of protein were then incubated for 2 h with shaking at 37°C with 100 μM TAMRA azide (Click Chemistry Tools Scottsdale, Az), 800 μM copper(II ...
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bioRxiv - Developmental Biology 2021Quote: ... stained using the CalFluor 647 Azide Kit (Click Chemistry Tools, Cat#: 1372), and imaged (see “Fluorescent tissue imaging/fluorescence image analysis”).
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bioRxiv - Bioengineering 2020Quote: ... or Click-&-Go™ Plus 647 Imaging Kit (Click Chemistry Tools, Scottsdale, AZ) preceding application of antibody staining solutions ...
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bioRxiv - Biochemistry 2020Quote: ... 1.6 mM BTTAA ligand (2-(4-((bis((1-tert-butyl-1H-1,2,3-triazol-4-yl)methyl)amino)methyl)−1 H-1,2,3-triazol-1-yl)acetic acid) (Click Chemistry Tools Scottsdale, Az), and 5 mM sodium ascorbate ...
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bioRxiv - Cell Biology 2024Quote: ... CalFluor 647 Azide(Click chemistry tools, 1272-1); Phosphatidylcholine Assay Kit (Colorimetric/Fluorometric ...
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bioRxiv - Bioengineering 2019Quote: ... 2,5-dioxopyrrolidin-1-yl 1-azido-3,6,9,12-tetraoxapentadecan-15-oate (azido-PEG4-NHS Ester) was purchased from Click Chemistry Tools (Scottsdale, AZ). Tris(3-hydroxypropyltriazolylmethyl)amine (THPTA) ...
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bioRxiv - Developmental Biology 2021Quote: ... 2 μM Cy3 azide (Click Chemistry Tools AZ119-1), and 100 mM ascorbic acid freshly prepared and diluted in Gomori buffer (pH 7.4) ...
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bioRxiv - Developmental Biology 2022Quote: ... The click reaction was performed for 1 hour in click staining solution (100 mM Tris pH 8.5, 1 mM CuSO4, 2 mM AZDye-488 Azide (Click Chemistry Tools, 1275-1), and 100 mM ascorbic acid) ...
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bioRxiv - Neuroscience 2021Quote: ... sections were click reacted using a Click&Go EdU 488 imaging kit (Click Chemistry Tools, #1324) according to manufacturer instructions before proceeding with subsequent immunolabeling ...
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bioRxiv - Microbiology 2022Quote: ... 5-EU was labeled in permeabilized cells using the Click & Go Kit (Click Chemistry Tools #1263) with 2 μM Alexa Fluor 488 azide (Click Chemistry tools #1275 ...
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bioRxiv - Cancer Biology 2023Quote: ... Monoclonal antibodies at 1 mg mL-1 in 1X PBS were incubated with DBCO-PEG5-NHS Ester linker (A102P, Click Chemistry Tools, Scottsdale, AZ, USA) at a 4:1 molar ratio of crosslinker to antibody for at least 2 h at room temperature ...
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bioRxiv - Microbiology 2023Quote: ... and 24 μM carboxyrhodamine 110 Alkyne (Click Chemistry Tools, TA106-1). Cells were washed 3x in PBS before acquisition via FACS or ImageStream.
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bioRxiv - Neuroscience 2020Quote: ... with 25 μM Alkyne Alexa fluor 405 (Click Chemistry tools # 1309-1), CuSO4 and additive overnight at 4°C ...
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bioRxiv - Biochemistry 2022Quote: ... and 100× molar equivalents of DBCO-AF647 (Click Chemistry Tools, 1302-1) in DMSO were added and the reaction was mixed at 500 rpm in the dark for 2 hours rt ...
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bioRxiv - Cell Biology 2022Quote: ... EdU was labelled using the 647 azide plus kit (Click-&-Go Plus EdU 647, Cat#1353, Click Chemistry Tools) according to the manufacturers protocol ...
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bioRxiv - Bioengineering 2023Quote: ... Sectioned gels were stained for EdU using a 647 fluorescent EdU kit (Click-&-Go EdU 647, Click Chemistry tools) per the manufacturer’s directions ...
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bioRxiv - Cell Biology 2024Quote: ... incorporated EdU was labeled using the Click-&-Go Plus EdU Cell Proliferation Kit for Imaging (Click Chemistry tools Catalog #1314) and imaged with a Leica THUNDER microscope ...
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bioRxiv - Biochemistry 2022Quote: ... An on bead click chemistry reaction was accomplished using 100 µl Click-&-Go click chemistry reaction buffer kit and 40 µM azide-biotin as per the manufacturer’s instructions (Click Chemistry Tools), and incubated in the dark at room temperature for 30 mins with gentle agitation ...
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bioRxiv - Neuroscience 2020Quote: ... the neurons were subjected to the FUNCAT reaction for 2 hours where the newly-synthesized AHA incorporated proteins were tagged with an alkyne-fluorophore Alexa-Fluor 555 through click reaction (C10269, CLICK-iT cell reaction buffer kit, Click Chemistry Tools). After 3 washes with TBS50T (0.1%) ...
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bioRxiv - Cell Biology 2021Quote: ... and labeled with 0.5 µM AF488-Picolyl Azide using the Click-&-Go Cell Reaction Buffer kit according to the manufacturers’ instructions (all reagents from Click Chemistry Tools). Cells were subsequently immunolabeled for TDP-43 as described above.
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bioRxiv - Neuroscience 2020Quote: ... the neurons were subjected to the FUNCAT reaction for 2 hours where the newly-synthesized AHA incorporated proteins were tagged with an alkyne-fluorophore Alexa-Fluor 555 through click reaction (C10269, CLICK-iT cell reaction buffer kit, Click Chemistry Tools). After 3 washes with TBS50t ...
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bioRxiv - Bioengineering 2020Quote: ... incubated for 40 minutes in AFDye 488 DBCO (Click Chemistry Tools, 1278-1; 30 μM) at 37°C ...
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bioRxiv - Biochemistry 2022Quote: ... MCF10A cells were treated with 1 mM 5-ethynyl uridine (5-EU; Click Chemistry Tools 1261) for 1 h to label nascent RNA as in (Bryant et al ...
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bioRxiv - Bioengineering 2023Quote: ... they were reacted with 10:1 Methyltetrazine-sulfo-N-Hydroxysuccinimide ester (Met-NHS) (Click Chemistry Tools):Peptides for an hour at room temperature in Dulbecco’s Phosphate Buffered Saline (DPBS ...
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bioRxiv - Biophysics 2024Quote: ... IEDDA reaction was performed by applying 1-2 μM tetrazine dye (Click Chemistry Tools, Scottsdale, AZ) to the culture medium and incubating it with the cells at 37°C for 30 minutes ...
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bioRxiv - Biochemistry 2023Quote: ... εmax = 250,000 M-1cm-1 for Cy5 as provided in the product specifications by Click Chemistry Tools. MWFis1ΔN = 13,584 daltons and MWDrp1 = 81,877 daltons.
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bioRxiv - Cell Biology 2022Quote: ... In every replicate 1 – 2 mg BTD-labeled lysate was clicked to Az800 (AzDye 800 – Click Chemistry tools) as described above ...
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bioRxiv - Microbiology 2023Quote: ... Bacteria were pulse-labeled for 60 min by supplementing 1 mM L-AHA (Click Chemistry Tools, 1066-100) or L-methionine (Sigma ...
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bioRxiv - Molecular Biology 2024Quote: ... The 3’-amino modified oligos (Supplementary Table S5) were activated by 1 mM NHS-methyltetrazine (Click Chemistry Tools) in 50% DMSO for 60 min at 21 °C ...