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Citations for Click Chemistry Tools :
51 - 96 of 96 citations for Monoisononyl Phthalate 100 Ug Ml In Mtbe Ring 1 2 13C2 Dicarboxyl 13C2 99% since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2023Quote: ... Azide-PEG4-NHS ester (Cat# AZ103-100) and Sulfo-DBCO NHS ester (Cat# A124-10) were purchased from Click Chemistry Tools (Scottsdale, AZ). Alexa 488 Mouse Anti-ZAP70 (pY319)/Syk (Y352 ...
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bioRxiv - Genetics 2024Quote: ... permeabilized with 0.5% TritonX-100 for 15 minutes at room temperature and subjected to click labeling reaction using AFDye 488 Azide (Click Chemistry Tools, #1314). The AFDye 488-labeled cells were analyzed by flow cytometry using the FITC channel.
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bioRxiv - Bioengineering 2020Quote: ... cytokines were reacted with a commercial 2-nitrobenzyl linker displaying both NHS ester and DBCO substituents (1160-10, Click Chemistry Tools) followed by addition of poly(ethylene glycol ...
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bioRxiv - Biochemistry 2019Quote: ... artificial amino acid-containing base was incubated at room temperature with 150 µM 5,5′-dithiobis-2-nitrobenzoic acid for 10 minutes before chilling on ice and adding 300 µM DBCO-Cy3 (Click Chemistry Tools) and incubating at 4°C overnight ...
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bioRxiv - Plant Biology 2023Quote: ... 44.5 μL of labelled proteins were incubated with 2 μM Cy5 Picolyl Azide (Click Chemistry Tools, 50 μM stock in DMSO) or Fluorescein Picolyl Azide (Click Chemistry Tools ...
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bioRxiv - Plant Biology 2024Quote: ... 44.5 μl of labelled proteins were treated with 2 μM Cy5 Picolyl Azide (Click Chemistry Tools, 50 μM stock in DMSO) or Fluorescein Picolyl Azide (Click Chemistry Tools ...
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bioRxiv - Cell Biology 2022Quote: ... were transfected with pcDNA3.1-SARS CoV-2-Spike-C9 and incubated overnight in DMEM supplemented with 10% (v/v) charcoal-stripped FBS with or without 100 μM of clickable fatty acid analogs (Click Chemistry Tools, Scottsdale, AZ) coupled to albumin ...
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bioRxiv - Biochemistry 2023Quote: ... 1 g lysate (500 µL) were reacted with click reagents to give final concentrations as follows: 100 µM of diazo biotin-azide (Click Chemistry Tools, Scottsdale, AZ), 800 µM copper (II ...
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bioRxiv - Biochemistry 2023Quote: ... 100 μg of lysate were reacted with click reagents to give final concentrations as follows: 100 µM of diazo biotin-azide (Click Chemistry Tools, Scottsdale, AZ), 800 µM copper (II ...
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bioRxiv - Biochemistry 2023Quote: ... 7.5 µL ‘click chemistry master mix’ was added to each sample to give final concentrations of 100 µM of Cy5-azide (Click Chemistry Tools, Scottsdale, AZ), 800 µM copper (II ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: PTH peptides with C terminal Cys residues purified by HPLC were incubated with 3 molar equivalents of DBCO-maleimide (Click Chemistry Tools #A108-100) and the DBCO-peptide products purified by HPLC ...
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bioRxiv - Immunology 2020Quote: ... A 500 μL labeling reaction containing 0.5 mg of ovalbumin in 1X BBS and 50 μL of 2 mM mTz-PEG4-NHS in DMSO (0.2 mM final; Click Chemistry Tools, 1069), pH 8.5 was rotated at 4 °C overnight ...
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bioRxiv - Bioengineering 2023Quote: 7-(Diethylamino)-4- (hydroxymethyl)coumarin (DEAC-OH) was previously synthesized in-house (Method S3) and conjugated to 2-azidoacetic acid (Click Chemistry tools, 1081), 3-azidopropionic acid (Synthonix ...
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bioRxiv - Biochemistry 2020Quote: ... 1.6 mM BTTAA ligand (2-(4-((bis((1-tert-butyl-1H-1,2,3-triazol-4-yl)methyl)amino)methyl)−1 H-1,2,3-triazol-1-yl)acetic acid) (Click Chemistry Tools Scottsdale, Az), and 5 mM sodium ascorbate ...
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bioRxiv - Cell Biology 2024Quote: ... CalFluor 647 Azide(Click chemistry tools, 1272-1); Phosphatidylcholine Assay Kit (Colorimetric/Fluorometric ...
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bioRxiv - Bioengineering 2019Quote: ... 2,5-dioxopyrrolidin-1-yl 1-azido-3,6,9,12-tetraoxapentadecan-15-oate (azido-PEG4-NHS Ester) was purchased from Click Chemistry Tools (Scottsdale, AZ). Tris(3-hydroxypropyltriazolylmethyl)amine (THPTA) ...
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bioRxiv - Developmental Biology 2020Quote: ... ESC were treated with DOX (different concentrations in the range from 150–600 ng/ml) or 500 µM auxin as indicated or incubate with 10µM EdU (Click Chemistry Tools) for 30 minutes before fixation with 4% PFA in PBS for 10 minutes at room temperature ...
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bioRxiv - Biochemistry 2022Quote: ... RPA-DBD-A4AZP or RPA-DBD-D4AZP (∼4 mM in 5 ml storage buffer) was mixed with 1.5-fold molar excess DBCO-Cy5 or DBCO-Cy3 (Click Chemistry Tools Inc.) and incubated for 2 hours at 4 °C ...
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bioRxiv - Biochemistry 2023Quote: 10 μM of RPA4AZP (0.5 mL) was mixed with 2X molar excess of the dibenzocyclooctyne-MB543 (DBCO-MB543; Click Chemistry Tools Inc.) dye and incubated at 4 °C for 1.5 h with gentle rocking in dark ...
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bioRxiv - Microbiology 2023Quote: ... and 24 μM carboxyrhodamine 110 Alkyne (Click Chemistry Tools, TA106-1). Cells were washed 3x in PBS before acquisition via FACS or ImageStream.
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bioRxiv - Molecular Biology 2021Quote: ... RPAAZP (∼ 4 µM in 5 mL of storage buffer) was mixed with 1.5-fold molar excess DBCO-MB543 (Click Chemistry Tools Inc., AZ) or DBCO-Cy3 ...
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bioRxiv - Biochemistry 2023Quote: ... 330 μL TCEP (14.0 mg/mL in H2O) and 160 μL of either heavy or light isoDTB tags (4 mg in DMSO, Click Chemistry Tools, 1565). The samples were then treated with 120 μL of the heavy (DMSO treated ...
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bioRxiv - Biochemistry 2024Quote: ... 165 µL TCEP (14.0 mg/mL in H2O) and 160 µL of either heavy or light isoDTB tags (4 mg in DMSO, Click Chemistry Tools, 1565). The samples were then treated with 120 µL of the heavy (DMSO treated ...
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bioRxiv - Neuroscience 2020Quote: ... with 25 μM Alkyne Alexa fluor 405 (Click Chemistry tools # 1309-1), CuSO4 and additive overnight at 4°C ...
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bioRxiv - Cancer Biology 2024Quote: ... protein lysates from OSU-CLL cells (1e6 cells/mL) were clicked to TAMRA biotin-azide and incubated with streptavidin agarose resin (Click Chemistry Tools; Scottsdale, AZ) to isolate biotin-alkyne-tagged proteins ...
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bioRxiv - Bioengineering 2020Quote: ... incubated for 40 minutes in AFDye 488 DBCO (Click Chemistry Tools, 1278-1; 30 μM) at 37°C ...
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bioRxiv - Biochemistry 2022Quote: ... MCF10A cells were treated with 1 mM 5-ethynyl uridine (5-EU; Click Chemistry Tools 1261) for 1 h to label nascent RNA as in (Bryant et al ...
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bioRxiv - Bioengineering 2023Quote: ... they were reacted with 10:1 Methyltetrazine-sulfo-N-Hydroxysuccinimide ester (Met-NHS) (Click Chemistry Tools):Peptides for an hour at room temperature in Dulbecco’s Phosphate Buffered Saline (DPBS ...
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bioRxiv - Biochemistry 2023Quote: ... εmax = 250,000 M-1cm-1 for Cy5 as provided in the product specifications by Click Chemistry Tools. MWFis1ΔN = 13,584 daltons and MWDrp1 = 81,877 daltons.
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bioRxiv - Molecular Biology 2024Quote: ... The 3’-amino modified oligos (Supplementary Table S5) were activated by 1 mM NHS-methyltetrazine (Click Chemistry Tools) in 50% DMSO for 60 min at 21 °C ...
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bioRxiv - Bioengineering 2019Quote: ... The suspension was centrifuged and resuspended in 1% Tween 80 containing PBS with methyltetrazine-PEG4-Maleimide (Click Chemistry Tools) of 200 μM final concentration ...
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bioRxiv - Bioengineering 2021Quote: ... 2-(4-((bis((1-(tert-butyl)-1H-1,2,3-triazol-4-yl)methyl)amino)methyl)-1H-1,2,3-triazol-1-yl)acetic acid (BTTAA, Click Chemistry Tools > 95%), Click-IT™ Fucose Alkyne (Invitrogen) ...
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bioRxiv - Bioengineering 2023Quote: ... and 2-(4-((bis((1-tert-butyl-1H-1,2,3-triazol-4-yl)methyl)amino)methyl)-1H-1,2,3-triazol-1-yl) acetic acid (BTTAA) (catalog no. 1236) were purchased from Click Chemistry Tools. Iodoacetamide and 4,5-methylenedioxy-1,2-phenylenediamine dihydrochloride (DMB ...
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bioRxiv - Molecular Biology 2024Quote: ... 25 µl of methanol-preserved cells (∼30’000 cells in total) were mixed with 1 mM NHS-TCO (Click Chemistry Tools) and incubated in the dark at 21 °C for 5 minutes ...
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bioRxiv - Bioengineering 2020Quote: ... The reduced C-terminal cysteine (1 eq.) was reacted with sulfo DBCO-maleimide crosslinker (4 eq.) (Click Chemistry Tools, AZ, USA) in PBS (pH 6.5 ...
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bioRxiv - Microbiology 2021Quote: ... Cells were resuspended in 50 μl of CuAAC reaction mixture (128 μM TBTA, 1 mM CuSO4, 1.2 mM freshly prepared sodium ascorbate, and 20 μM AFDye 488 Azide Plus fluorophore [Click Chemistry Tools] in PBS + 0.1% Triton X-100 + 0.01% BSA ...
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bioRxiv - Bioengineering 2021Quote: ... two HBSS washes were performed and hydrogels were then incubated in AFDye 488 DBCO (Click Chemistry Tools, 1278-1; 30 μM) for 40 minutes at 37°C ...
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bioRxiv - Cell Biology 2022Quote: ... The beads were then washed with ice-cold D-PBS 4 times and incubated in D-PBS supplemented with 1 μM Cy5.5-azide (Click Chemistry Tools), 1 mM TCEP (adjusted to pH 7.0) ...
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bioRxiv - Molecular Biology 2022Quote: Cells were washed 3X with PBS/1%FBS (pH 7.4) followed by treatment with 10 μM CalFluor 647 Azide (Click Chemistry Tools), premixed BTTAA-CuSO4 complex ([BTTAA]:[CuSO4]=2:1 ...
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bioRxiv - Molecular Biology 2022Quote: ... Cells were washed 3X with PBS/1%FBS (pH 7.4) followed by treatment with 10 μM CalFluor 647 Azide (Click Chemistry Tools), premixed BTTAA-CuSO4 complex ([BTTAA]:[CuSO4]=2:1 ...
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bioRxiv - Microbiology 2023Quote: ... Cells were resuspended in 50 μl of CuAAC reaction mixture (128 μM TBTA, 1 mM CuSO4, 1.2 mM freshly prepared sodium ascorbate, and 20 μM AFDye 488 Azide Plus fluorophore [Click Chemistry Tools] in PBS + 0.1% Triton X-100 + 0.01% BSA ...
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Bio-orthogonal Glycan Imaging of Culture Cells and Whole Animal C. elegans with Expansion MicroscopybioRxiv - Bioengineering 2024Quote: ... elegans eggs were hatched into L1 larvae for 24 h without food in M9 buffer containing 1 mM tetra-acylated azidoacetylgalactosamine (GalNAz, Click Chemistry Tools) or M9 buffer containing equal volume of the vehicle (DMSO) ...
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bioRxiv - Cell Biology 2023Quote: ... were dissolved in dry DMF (200 µL) in a plastic screw cap tube and 3-azidopropyl-1-amine (11 μL, 110 μmol, Click Chemistry Tools) followed by DIEA (38 μL ...
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bioRxiv - Microbiology 2019Quote: ... with an azide or alkyne Alexa Fluor 488/594 (AF 594 Alkyne, 1297-1 or AF 488 Picolyl Azide 1276-5, Click Chemistry Tools, USA) for 1 hour in the dark ...
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bioRxiv - Bioengineering 2024Quote: ... whereas PEG-BCN was preincubated for 15 minutes with 50 µM of AlexaFluor 568 azide (1:200 dye:PEG) (Click Chemistry Tools; Scottsdale, AZ). DN hydrogels were then formed with these fluorescently tagged components ...
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bioRxiv - Microbiology 2022Quote: ... L-AHA-containing proteins were labeled with Cy7 by strain-promoted azide-alkyne click-chemistry employing 1 μM dibenzylcyclooctyne-Cy7 (DBCO-Cy7) (Click Chemistry Tools, USA, Scottsdale). After methanol-chloroform extraction ...