Labshake search
Citations for Click Chemistry Tools :
1 - 50 of 54 citations for Creatinine Urinary Detection Kit 10 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Genomics 2022Quote: ... 10 µL of 10 mM DBCO-NHS (Click Chemistry Tools cat# A134-10) was then added to the antibodies and allowed to rotate at room temperature for one hour 26 ...
-
bioRxiv - Neuroscience 2021Quote: ... 10 μM AFDye 488 Azide (Click Chemistry Tools), and 100 mM ascorbic acid in the dark at room temperature for 30 minutes ...
-
bioRxiv - Bioengineering 2022Quote: ... NHS-PEG-TCO ester (A137-10, Click Chemistry Tools), poly(ethylene glycol ...
-
bioRxiv - Molecular Biology 2021Quote: The Click reaction was then performed by incubating cells with 100 µL Click buffer (100 µM copper sulfate, 2 mM THPTA, 10 mM fresh sodium ascorbate, 10 µM Cy5 picolyl azide (Click Chemistry Tools 1171-1)) for 1 hour at 37°C in the dark ...
-
bioRxiv - Genomics 2022Quote: 100 µl of 100 µM oligo barcode (IDT) in PBS and 10 µl of 10 mM azide-NHS (Click Chemistry Tools cat# 1251-5) in DMSO were mixed at room temperature for two hours ...
-
bioRxiv - Molecular Biology 2022Quote: ... in situ click reactions were performed by incubating cells with 100 µL Click buffer (100 µM copper sulfate, 2 mM THPTA, 10 mM fresh sodium ascorbate, 10 µM Cy3 picolyl azide (Click Chemistry Tools 1178-1)) for 1 hour at 37°C in the dark ...
-
bioRxiv - Molecular Biology 2021Quote: ... and 1.5 µl of 10 mM Azide-PEG4-Biotin (Click Chemistry Tools), the click reaction was conducted for 60 min at room temperature ...
-
bioRxiv - Bioengineering 2021Quote: ... Cy5.5-NHS ester (purchased from Click Chemistry Tools, for Lys-cy5.5 conjugate 10), or DBCO–Cy5.5 (purchased from Click Chemistry Tools ...
-
bioRxiv - Molecular Biology 2023Quote: ... were incubated with 10 mM biotin azide (probe condition; Click Chemistry Tools 1265) or 10 mM desthio-biotin (no probe condition ...
-
bioRxiv - Bioengineering 2023Quote: ... in PBS and incubated in 10 μM AZDye 488 DBCO (Click Chemistry Tools) for 30 min at 37 °C ...
-
bioRxiv - Bioengineering 2024Quote: ... gels were soaked in 10 µM Cy5-N3 (Click Chemistry Tools; Scottsdale, AZ) for 2 hours at 37 °C to block any unreacted BCN groups ...
-
bioRxiv - Bioengineering 2024Quote: ... gels were soaked in 10 µM Cy5-N3 (Click Chemistry Tools; Scottsdale, AZ) for 2 hours at 37 °C to label the SPAAC network and then washed 3 x 1 hr in PBS ...
-
bioRxiv - Cancer Biology 2022Quote: ... 5 μl DBCO-S-S-biotin (Click Chemistry Tools, 10 mM stock in DMSO) was directly added to the reaction mixture and incubated overnight at 37°C ...
-
bioRxiv - Cancer Biology 2022Quote: ... 5 μl DBCO-S-S-biotin (Click Chemistry Tools, 10 mM stock in DMSO) was directly added to the reaction mixture and incubated overnight at 37°C ...
-
bioRxiv - Bioengineering 2022Quote: ... 50 μL of antibodies were reacted with 10 mM photocleavable azido-NHS ester (Click Chemistry Tools) at 1 ...
-
bioRxiv - Synthetic Biology 2020Quote: ... SpoIVA was labelled with 10-fold molar excess of trans-cyclooctene-PEG3-Maleimide (Click Chemistry Tools) overnight at 4 °C ...
-
bioRxiv - Bioengineering 2023Quote: ... they were reacted with 10:1 Methyltetrazine-sulfo-N-Hydroxysuccinimide ester (Met-NHS) (Click Chemistry Tools):Peptides for an hour at room temperature in Dulbecco’s Phosphate Buffered Saline (DPBS ...
-
bioRxiv - Immunology 2020Quote: ... 8 μL of 100 mM TCO-PEG4-NHS in DMSO (10 mM final; Click Chemistry Tools, A137), pH 8.5 was rotated at room temperature for 15 min ...
-
bioRxiv - Bioengineering 2022Quote: ... and Click-&-Go Cell Reaction Buffer Kit (Click Chemistry Tools), and 3 ...
-
bioRxiv - Microbiology 2023Quote: ... using the Click-&-Go protein enrichment kit (Click Chemistry Tools) according to the manufacturer’s protocol ...
-
bioRxiv - Biochemistry 2020Quote: ... is combined with DBCO-Peg5-NHS Ester (linker; 10 mM in DMSO; Click Chemistry Tools Cat: 1378531-80-6) in 60 molar excess (50 mg of Rabbit IgG and 4.6 mg of linker) ...
-
bioRxiv - Neuroscience 2024Quote: ... were submerged in dimethyl sulfoxide (DMSO) with 10 mM dibenzocyclooctyne-PEG-amine (DBCO-PEG4-amine; Click Chemistry Tools, A103P) overnight at room temperature ...
-
bioRxiv - Developmental Biology 2021Quote: ... stained using the CalFluor 647 Azide Kit (Click Chemistry Tools, Cat#: 1372), and imaged (see “Fluorescent tissue imaging/fluorescence image analysis”).
-
bioRxiv - Synthetic Biology 2023Quote: ... and dibenzocyclooctyne-sulfo-N-hydroxysuccinimide ester (DBCO-Sulfo-NHS ester, Click Chemistry Tools, prepared as 10 mM stock in DMSO) at a 2500:1:1000 molar ratio of dye:particle:DBCO ...
-
bioRxiv - Bioengineering 2024Quote: ... 40 µL of 10 mM Cy7-NHS Ester (#1115) or AZDye 488-NHS Ester (#1338) (both from Click Chemistry Tools) in DMSO was added to the solution dropwise in dark at 4 °C ...
-
bioRxiv - Bioengineering 2020Quote: ... cytokines were reacted with a commercial 2-nitrobenzyl linker displaying both NHS ester and DBCO substituents (1160-10, Click Chemistry Tools) followed by addition of poly(ethylene glycol ...
-
bioRxiv - Biochemistry 2019Quote: ... artificial amino acid-containing base was incubated at room temperature with 150 µM 5,5′-dithiobis-2-nitrobenzoic acid for 10 minutes before chilling on ice and adding 300 µM DBCO-Cy3 (Click Chemistry Tools) and incubating at 4°C overnight ...
-
bioRxiv - Molecular Biology 2022Quote: Cells were washed 3X with PBS/1%FBS (pH 7.4) followed by treatment with 10 μM CalFluor 647 Azide (Click Chemistry Tools), premixed BTTAA-CuSO4 complex ([BTTAA]:[CuSO4]=2:1 ...
-
bioRxiv - Molecular Biology 2022Quote: ... Cells were washed 3X with PBS/1%FBS (pH 7.4) followed by treatment with 10 μM CalFluor 647 Azide (Click Chemistry Tools), premixed BTTAA-CuSO4 complex ([BTTAA]:[CuSO4]=2:1 ...
-
bioRxiv - Molecular Biology 2024Quote: ... The reaction was terminated by quenching with a 10 mM Tris-HCl [pH 8.0] buffer supplemented with 50 µM methyltetrazine-DBCO (Click Chemistry Tools). The methanol-preserved cells conjugated with ClickTags were rehydrated following Supplementary Protocol 3.
-
bioRxiv - Neuroscience 2020Quote: ... Alkyne-conjugated biotin and Protein Reaction Buffer kit were from Click Chemistry Tools.
-
bioRxiv - Bioengineering 2020Quote: ... or Click-&-Go™ Plus 647 Imaging Kit (Click Chemistry Tools, Scottsdale, AZ) preceding application of antibody staining solutions ...
-
bioRxiv - Molecular Biology 2019Quote: Biotin was attached to metabolically labeled RNAs in a 10–20 μl reaction with 4 mM biotin disulfide azide (Click Chemistry Tools), 5 mM CuSO4 (Sigma Aldrich) ...
-
bioRxiv - Bioengineering 2021Quote: ... 50 μL of 1 mg/mL antibodies were reacted with 1 μL of 10 mM UV-cleavable azido-NHS ester (30 equivalents to antibodies; Click Chemistry Tools) for 2 h ...
-
bioRxiv - Biochemistry 2022Quote: HEK293A cells with or without TEAD overexpression was pretreated with DMSO or TM2 in medium with 10% dialyzed fetal bovine serum (DFBS) for 8 hand labeled by Alkynyl Palmitic acid (1165, Click Chemistry Tools) for another 16 h ...
-
bioRxiv - Biochemistry 2023Quote: 10 μM of RPA4AZP (0.5 mL) was mixed with 2X molar excess of the dibenzocyclooctyne-MB543 (DBCO-MB543; Click Chemistry Tools Inc.) dye and incubated at 4 °C for 1.5 h with gentle rocking in dark ...
-
bioRxiv - Biochemistry 2023Quote: ... the substrate was diluted to 100 μM and incubated for 10 minutes at room temperature with 200 μM SulfoCy5 (for stopped-flow measurements, Click Chemistry Tools) or LD555 (for smFRET measurements) ...
-
bioRxiv - Biochemistry 2023Quote: ... was added to the purified base after concentrating and incubated for 10 min on ice before addition of 300 μM dybenzocyclooctane (DBCO)-Cy3 (Click Chemistry Tools) or LD655 (Lumidyne Technologies) ...
-
bioRxiv - Cell Biology 2021Quote: ... was conducted using the Click-&-Go Protein Reaction Buffer Kit (Click Chemistry Tools 1262) according to the manufacturer’s protocol ...
-
bioRxiv - Biophysics 2023Quote: ... Azide-PEG4-NHS ester (Cat# AZ103-100) and Sulfo-DBCO NHS ester (Cat# A124-10) were purchased from Click Chemistry Tools (Scottsdale, AZ). Alexa 488 Mouse Anti-ZAP70 (pY319)/Syk (Y352 ...
-
bioRxiv - Cell Biology 2022Quote: ... were transfected with pcDNA3.1-SARS CoV-2-Spike-C9 and incubated overnight in DMEM supplemented with 10% (v/v) charcoal-stripped FBS with or without 100 μM of clickable fatty acid analogs (Click Chemistry Tools, Scottsdale, AZ) coupled to albumin ...
-
bioRxiv - Neuroscience 2021Quote: ... sections were click reacted using a Click&Go EdU 488 imaging kit (Click Chemistry Tools, #1324) according to manufacturer instructions before proceeding with subsequent immunolabeling ...
-
bioRxiv - Microbiology 2022Quote: ... 5-EU was labeled in permeabilized cells using the Click & Go Kit (Click Chemistry Tools #1263) with 2 μM Alexa Fluor 488 azide (Click Chemistry tools #1275 ...
-
bioRxiv - Biochemistry 2020Quote: ... Enrichment of alkyne-modified proteins was conducted using the Click-&-Go Protein Enrichment Kit (Click Chemistry Tools) and the protocols thereof ...
-
bioRxiv - Cell Biology 2020Quote: ... The Click-iT reaction was performed with the Click-&-Go Protein Reaction Buffer Kit (Click Chemistry Tools) and AF488 alkyne (Click Chemistry Tools ...
-
bioRxiv - Microbiology 2020Quote: ... to AHA containing proteins was carried out using Click-&-Go™ Protein Reaction Buffer Kit (Click Chemistry Tools) according to the manufacturer’s instructions ...
-
bioRxiv - Cell Biology 2022Quote: ... EdU was labelled using the 647 azide plus kit (Click-&-Go Plus EdU 647, Cat#1353, Click Chemistry Tools) according to the manufacturers protocol ...
-
bioRxiv - Bioengineering 2023Quote: ... Sectioned gels were stained for EdU using a 647 fluorescent EdU kit (Click-&-Go EdU 647, Click Chemistry tools) per the manufacturer’s directions ...
-
bioRxiv - Cell Biology 2024Quote: ... incorporated EdU was labeled using the Click-&-Go Plus EdU Cell Proliferation Kit for Imaging (Click Chemistry tools Catalog #1314) and imaged with a Leica THUNDER microscope ...
-
bioRxiv - Biochemistry 2022Quote: ... An on bead click chemistry reaction was accomplished using 100 µl Click-&-Go click chemistry reaction buffer kit and 40 µM azide-biotin as per the manufacturer’s instructions (Click Chemistry Tools), and incubated in the dark at room temperature for 30 mins with gentle agitation ...