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Citations for Click Chemistry Tools :
1 - 50 of 52 citations for Caspase 8 CASP8 Antibody Biotin since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2020Quote: ... 3) 8 ul of 5mM Biotin-Alkyne (C21H35N3O6S, Click Chemistry Tools), and 4 ...
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bioRxiv - Biochemistry 2023Quote: ... and biotin-DADPS-azide (8) were purchased from Click Chemistry Tools. Full chemical structures of key reagents are shown in Fig ...
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bioRxiv - Neuroscience 2019Quote: ... we incubated the lysate with DBCO-biotin [8] (30 μM; Click Chemistry Tools LLC) for 1 hr at ambient temperature ...
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bioRxiv - Biochemistry 2022Quote: ... Stock solutions of biotin-tetrazine (biotin-PEG4-methyltetrazine, Click Chemistry Tools), bis-biotinamide (bis-dPEG3-biotin ...
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bioRxiv - Bioengineering 2020Quote: ... Azide-PC-Biotin (Click Chemistry Tools) dissolved in DMSO was added to the wells at 1 eq ...
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bioRxiv - Molecular Biology 2021Quote: ... 2 mM biotin azide (Click Chemistry Tools), 10 mM sodium ascorbate made fresh (Sigma) ...
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Chemoproteomics of microbiota metabolites reveals small-molecule agonists for orphan receptor GPRC5AbioRxiv - Biochemistry 2021Quote: ... azido-PEG3-biotin (Click Chemistry Tools, Catalog #AZ104).
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bioRxiv - Synthetic Biology 2023Quote: ... Antibodies were then co-incubated with a 20-fold molar excess of NHS ester: PC-biotin-NHS ester (Click Chemistry Tools), Biotin-PEG2-NHS ester (BroadPharma) ...
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bioRxiv - Biochemistry 2021Quote: ... Click chemistry reactants were freshly prepared as a 5X master mix that consists of 0.5 M biotin-azide (Biotin-Picolyl azide, 1167, Click Chemistry Tools), 5 mM TCEP ...
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bioRxiv - Molecular Biology 2019Quote: Biotin was attached to metabolically labeled RNAs in a 10–20 μl reaction with 4 mM biotin disulfide azide (Click Chemistry Tools), 5 mM CuSO4 (Sigma Aldrich) ...
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bioRxiv - Biochemistry 2022Quote: ... 100 uM Biotin-Azide (1167-5, Click Chemistry Tools) and incubated for another 1 h ...
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bioRxiv - Developmental Biology 2019Quote: ... 4 mM disulfide biotin azide (Click Chemistry Tools, Scottsdale, AZ) was added to a 10 μL reaction containing RNA ...
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bioRxiv - Neuroscience 2022Quote: ... 5 mM Biotin-PEG4-Alkyne (Click Chemistry Tools, Cat#TA105100) and 50 mM TCEP (Sigma ...
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bioRxiv - Neuroscience 2022Quote: ... to label EdU with Azide-biotin (Click Chemistry Tools #1265) for subsequent Streptavidin-555 (Invitrogen ...
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bioRxiv - Biochemistry 2022Quote: For the acid-cleavable reagent (DADPS Biotin Azide, CLICK CHEMISTRY TOOLS) ligated sample ...
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bioRxiv - Cell Biology 2023Quote: ... 0.4 μL of 50 mM WS DBCO Biotin (Click Chemistry Tools) was added ...
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bioRxiv - Molecular Biology 2021Quote: ... and 1.5 µl of 10 mM Azide-PEG4-Biotin (Click Chemistry Tools), the click reaction was conducted for 60 min at room temperature ...
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bioRxiv - Cancer Biology 2020Quote: ... 5 μl of 2 mM biotin-alkyne-tag (Click Chemistry Tools, 1266), and 5 μl of 200 mM CuSO4 were added in the specified order to 5 ml of PBS (pH 7.8) ...
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bioRxiv - Cell Biology 2021Quote: Labeling of the incorporated AHA by biotin-alkyne (Click Chemistry Tools 1266) was conducted using the Click-&-Go Protein Reaction Buffer Kit (Click Chemistry Tools 1262 ...
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bioRxiv - Neuroscience 2020Quote: ... Alkyne-conjugated biotin and Protein Reaction Buffer kit were from Click Chemistry Tools.
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bioRxiv - Molecular Biology 2020Quote: ... The lysates were reacted with 100 μM azide-PEG3-biotin (Click Chemistry Tools), 500 μM CuSO4 ...
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bioRxiv - Cancer Biology 2023Quote: ... Click reaction was performed to conjugate biotin-azide (Cat# 1265, Click Chemistry Tools) to EdU labeled nascent DNA and sonicated (Bioruptor Pico sonication device) ...
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bioRxiv - Molecular Biology 2023Quote: ... were incubated with 10 mM biotin azide (probe condition; Click Chemistry Tools 1265) or 10 mM desthio-biotin (no probe condition ...
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bioRxiv - Cancer Biology 2022Quote: ... 5 μl DBCO-S-S-biotin (Click Chemistry Tools, 10 mM stock in DMSO) was directly added to the reaction mixture and incubated overnight at 37°C ...
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bioRxiv - Cancer Biology 2022Quote: ... 5 μl DBCO-S-S-biotin (Click Chemistry Tools, 10 mM stock in DMSO) was directly added to the reaction mixture and incubated overnight at 37°C ...
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bioRxiv - Microbiology 2021Quote: ... Samples were subsequently incubated with 100 μM diazo biotin azide (azo-tag; Click Chemistry Tools), 1 mM tris(2-carboxyethyl)phosphine (TCEP ...
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Chemoproteomics of microbiota metabolites reveals small-molecule agonists for orphan receptor GPRC5AbioRxiv - Biochemistry 2021Quote: ... cell lysates prepared as above were labeled with azido-PEG3-biotin (Click Chemistry Tools, AZ104), incubated with high-capacity neutravidin beads (Thermo Scientific Pierce) ...
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bioRxiv - Genomics 2023Quote: ... The modified DNA was added to 1µl [1.0mM] Disulfide Biotin DBCO (Click Chemistry Tools A112-5) and incubated at 37°C for 2 hours ...
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bioRxiv - Cell Biology 2024Quote: AF488 picolyl azide and Dde biotin picolyl azide were purchased from Click Chemistry Tools (Scottsdale, AZ). DNA Isolation Midi-Prep Plus and DNA Clean and Concentrator Kits were purchased from Zymo Research ...
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bioRxiv - Cancer Biology 2022Quote: ... Biotin-alkyne (#1266-5) and TAMRA-alkyne (#1255-5) were purchased from Click Chemistry Tools (Scottsdale, AZ). O-GlcNAc Chemoenzymatic labeling using the Click-IT™ kit were carried out as recommended by the manufacturer (ThermoFisher) ...
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bioRxiv - Biochemistry 2021Quote: ... Supernatant was collected and incubated with 120 μM dialkoxydiphenylsilane (DADPS) alkyne-biotin (Click Chemistry Tools, #1331-25), 100 μM BTTAA (Click Chemistry Tools ...
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bioRxiv - Physiology 2023Quote: ... and 1.14 μl Biotin-N3 (stock solution: 9.67 mM in DMSO; 1265, Click Chemistry Tools, Scottsdale, AZ), were added for each reaction and this was incubated at room temperature for 1 hour ...
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bioRxiv - Microbiology 2021Quote: ... One mg of cell lysate was resuspended in 445µl 1x SDS buffer with 1x EDTA-free protease inhibitors and incubated for 1.5 hours with 55µl of click reaction master-mix consisting of 10µl of 5mM diazo biotin azide (Click Chemistry Tools), 10µl of 50mM TCEP ...
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bioRxiv - Biochemistry 2022Quote: ... 600 mM NaCl and then the azido-sialic acids were labeled with 30 μM WS DBCO-biotin (Click Chemistry Tools) at room temperature for 30 min ...
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bioRxiv - Biochemistry 2022Quote: ... An on bead click chemistry reaction was accomplished using 100 µl Click-&-Go click chemistry reaction buffer kit and 40 µM azide-biotin as per the manufacturer’s instructions (Click Chemistry Tools), and incubated in the dark at room temperature for 30 mins with gentle agitation ...
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bioRxiv - Neuroscience 2023Quote: ... Alkylated and cleaned sample of AHA/ANL incorporated samples were clicked to a disulfide-biotin alkyne (DST) (Click Chemistry Tools, #1498) as described before ...
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bioRxiv - Genomics 2024Quote: ... we performed a click reaction between the azide-linked modifications (products from the previous reaction) and the strained alkyne DBCO-PEG4-biotin (Click Chemistry Tools). The resulting biotinylated-5hmC-modified cfDNA fragments were then affinity captured using Dynabeads M-270 Streptavidin (Thermo) ...
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bioRxiv - Biochemistry 2023Quote: ... 1 g lysate (500 µL) were reacted with click reagents to give final concentrations as follows: 100 µM of diazo biotin-azide (Click Chemistry Tools, Scottsdale, AZ), 800 µM copper (II ...
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bioRxiv - Biochemistry 2023Quote: ... 100 μg of lysate were reacted with click reagents to give final concentrations as follows: 100 µM of diazo biotin-azide (Click Chemistry Tools, Scottsdale, AZ), 800 µM copper (II ...
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bioRxiv - Cancer Biology 2024Quote: ... protein lysates from OSU-CLL cells (1e6 cells/mL) were clicked to TAMRA biotin-azide and incubated with streptavidin agarose resin (Click Chemistry Tools; Scottsdale, AZ) to isolate biotin-alkyne-tagged proteins ...
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bioRxiv - Immunology 2020Quote: ... 8 μL of 100 mM TCO-PEG4-NHS in DMSO (10 mM final; Click Chemistry Tools, A137), pH 8.5 was rotated at room temperature for 15 min ...
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bioRxiv - Biochemistry 2022Quote: HEK293A cells with or without TEAD overexpression was pretreated with DMSO or TM2 in medium with 10% dialyzed fetal bovine serum (DFBS) for 8 hand labeled by Alkynyl Palmitic acid (1165, Click Chemistry Tools) for another 16 h ...
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bioRxiv - Bioengineering 2021Quote: ... 50 μL of 1 mg/mL antibodies were reacted with 1 μL of 10 mM UV-cleavable azido-NHS ester (30 equivalents to antibodies; Click Chemistry Tools) for 2 h ...
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bioRxiv - Cancer Biology 2024Quote: ... Antibodies were conjugated to mTz-PEG4-NHS (Click chemistry tools) at first in 1x BBS ...
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bioRxiv - Molecular Biology 2020Quote: ... Antibody was modified with DBCO-Sulfo-NHS ester (Click Chemistry Tools, #A124) according to manufacturer’s instructions ...
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bioRxiv - Bioengineering 2022Quote: ... 50 μL antibodies were then modified with UV-cleavable azido-NHS ester (Click Chemistry Tools) at 1:15 molar ratio for 2 h ...
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bioRxiv - Cancer Biology 2020Quote: ... Antibodies were incubated with DBCO-PEG5-NHS Ester linker (Click Chemistry Tools, cat. no. A102P) at a 4:1 molar ratio linker:antibody for 2 h at room temperature ...
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bioRxiv - Bioengineering 2022Quote: ... 50 μL of antibodies were reacted with 10 mM photocleavable azido-NHS ester (Click Chemistry Tools) at 1 ...
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bioRxiv - Immunology 2024Quote: ... Successful attachment of azide groups to antibodies was confirmed by a test conjugation with DBCO-AZDye (Click Chemistry Tools/Vector Laboratories) followed by detection of fluor-conjugated antibodies using UltraComp eBeads (ThermoFisher) ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Antibodies were functionalized by reacting with a 5-fold molar excess of dibenzocyclooctyne-PEG4-NHS ester (DBCO-PEG4-NHS) (Click Chemistry Tools, Scottsdale, AZ) for 30 minutes at room temperature ...