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Citations for Click Chemistry Tools :
1 - 50 of 76 citations for 7H Purine 7 acetamide N dibenzofuran 3 yl 1 2 3 6 tetrahydro 1 3 dimethyl 2 6 dioxo 9CI since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2022Quote: ... and 5-days post-surgery along with 5-Ethynyl-2′-deoxyuridine (EdU, Click Chemistry Tools, 3 mg/kg) every day (n = 4-5/group) ...
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bioRxiv - Biochemistry 2020Quote: ... 1.6 mM BTTAA ligand (2-(4-((bis((1-tert-butyl-1H-1,2,3-triazol-4-yl)methyl)amino)methyl)−1 H-1,2,3-triazol-1-yl)acetic acid) (Click Chemistry Tools Scottsdale, Az), and 5 mM sodium ascorbate ...
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bioRxiv - Molecular Biology 2024Quote: ... The 3’-amino modified oligos (Supplementary Table S5) were activated by 1 mM NHS-methyltetrazine (Click Chemistry Tools) in 50% DMSO for 60 min at 21 °C ...
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bioRxiv - Bioengineering 2021Quote: ... 2-(4-((bis((1-(tert-butyl)-1H-1,2,3-triazol-4-yl)methyl)amino)methyl)-1H-1,2,3-triazol-1-yl)acetic acid (BTTAA, Click Chemistry Tools > 95%), Click-IT™ Fucose Alkyne (Invitrogen) ...
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bioRxiv - Bioengineering 2021Quote: ... 5 mM tris(3-hydroxypropyltriazolylmethyl)amine (THPTA; Click Chemistry Tools), 2 mM copper sulfate ...
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bioRxiv - Cell Biology 2023Quote: ... were dissolved in dry DMF (100 µL) in a plastic screw cap tube and 3-azidopropyl-1-amine (3.7 μL, 37.2 μmol, Click Chemistry Tools) followed by DIEA (13 μL ...
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bioRxiv - Neuroscience 2020Quote: ... 3) 8 ul of 5mM Biotin-Alkyne (C21H35N3O6S, Click Chemistry Tools), and 4 ...
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bioRxiv - Cell Biology 2023Quote: ... were dissolved in dry DMF (200 µL) in a plastic screw cap tube and 3-azidopropyl-1-amine (11 μL, 110 μmol, Click Chemistry Tools) followed by DIEA (38 μL ...
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bioRxiv - Biochemistry 2023Quote: ... Tris(3-hydroxypropyltriazolyl-methyl)amine (THPTA) was purchased from Click Chemistry Tools. 1-Palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC) ...
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bioRxiv - Developmental Biology 2021Quote: ... 2 μM Cy3 azide (Click Chemistry Tools AZ119-1), and 100 mM ascorbic acid freshly prepared and diluted in Gomori buffer (pH 7.4) ...
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bioRxiv - Bioengineering 2019Quote: ... 2,5-dioxopyrrolidin-1-yl 1-azido-3,6,9,12-tetraoxapentadecan-15-oate (azido-PEG4-NHS Ester) was purchased from Click Chemistry Tools (Scottsdale, AZ). Tris(3-hydroxypropyltriazolylmethyl)amine (THPTA) ...
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bioRxiv - Bioengineering 2021Quote: ... 6-Azidohexanoic Acid Ester (Click Chemistry tools, > 95%), Alkyne-PEG4-NHS Ester (Click Chemistry Tools > 95%) ...
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bioRxiv - Bioengineering 2023Quote: ... and 2-(4-((bis((1-tert-butyl-1H-1,2,3-triazol-4-yl)methyl)amino)methyl)-1H-1,2,3-triazol-1-yl) acetic acid (BTTAA) (catalog no. 1236) were purchased from Click Chemistry Tools. Iodoacetamide and 4,5-methylenedioxy-1,2-phenylenediamine dihydrochloride (DMB ...
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bioRxiv - Biophysics 2024Quote: ... IEDDA reaction was performed by applying 1-2 μM tetrazine dye (Click Chemistry Tools, Scottsdale, AZ) to the culture medium and incubating it with the cells at 37°C for 30 minutes ...
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bioRxiv - Developmental Biology 2022Quote: ... The click reaction was performed for 1 hour in click staining solution (100 mM Tris pH 8.5, 1 mM CuSO4, 2 mM AZDye-488 Azide (Click Chemistry Tools, 1275-1), and 100 mM ascorbic acid) ...
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bioRxiv - Cell Biology 2022Quote: ... In every replicate 1 – 2 mg BTD-labeled lysate was clicked to Az800 (AzDye 800 – Click Chemistry tools) as described above ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: PTH peptides with C terminal Cys residues purified by HPLC were incubated with 3 molar equivalents of DBCO-maleimide (Click Chemistry Tools #A108-100) and the DBCO-peptide products purified by HPLC ...
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bioRxiv - Molecular Biology 2021Quote: ... 2 mM THPTA (Click Chemistry Tools), and 100 µM copper sulfate ...
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bioRxiv - Bioengineering 2023Quote: ... they were reacted with 10:1 Methyltetrazine-sulfo-N-Hydroxysuccinimide ester (Met-NHS) (Click Chemistry Tools):Peptides for an hour at room temperature in Dulbecco’s Phosphate Buffered Saline (DPBS ...
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bioRxiv - Molecular Biology 2021Quote: ... 2 mM biotin azide (Click Chemistry Tools), 10 mM sodium ascorbate made fresh (Sigma) ...
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bioRxiv - Physiology 2023Quote: ... strain-promoted inverse electron-demand Diels-Alder cycloaddition (SPIEDAC) was performed by adding 1-2 μM tetrazine dye (Click Chemistry Tools, Scottsdale, AZ) to the culture medium and incubating it with the cells at 37°C for 30 minutes ...
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bioRxiv - Molecular Biology 2021Quote: The Click reaction was then performed by incubating cells with 100 µL Click buffer (100 µM copper sulfate, 2 mM THPTA, 10 mM fresh sodium ascorbate, 10 µM Cy5 picolyl azide (Click Chemistry Tools 1171-1)) for 1 hour at 37°C in the dark ...
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bioRxiv - Molecular Biology 2022Quote: ... in situ click reactions were performed by incubating cells with 100 µL Click buffer (100 µM copper sulfate, 2 mM THPTA, 10 mM fresh sodium ascorbate, 10 µM Cy3 picolyl azide (Click Chemistry Tools 1178-1)) for 1 hour at 37°C in the dark ...
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bioRxiv - Molecular Biology 2023Quote: ... is incubated with DBCO-Peg5-NHS Ester (linker; 10mM in DMSO; Click Chemistry Tools Cat: 1378531-80-6) in 60 molar excess (10 μg of antibody and 2.8 μg of linker ...
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bioRxiv - Molecular Biology 2022Quote: ... 2 mM THPTA (Click Chemistry Tools 1010-100), and 100 µM copper sulfate (Fisher Scientific AC197722500) ...
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bioRxiv - Biochemistry 2020Quote: ... is combined with DBCO-Peg5-NHS Ester (linker; 10 mM in DMSO; Click Chemistry Tools Cat: 1378531-80-6) in 60 molar excess (50 mg of Rabbit IgG and 4.6 mg of linker) ...
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bioRxiv - Genetics 2022Quote: ... 5-ethynyl-2’-deoxyuridine (EdU) (Click Chemistry Tools 1381, Scottsdale, AZ) was added to a final concentration of 10 uM ...
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bioRxiv - Microbiology 2022Quote: ... with 2 μM Alexa Fluor 488 azide (Click Chemistry tools #1275) according to manufacturer instructions ...
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bioRxiv - Neuroscience 2022Quote: ... 20 µM 5-Ethynyl-2′-deoxyuridine (EdU; Click Chemistry Tools 1149) was added to the cell culture media for 30 minutes to label NSCs in S-phase ...
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bioRxiv - Cancer Biology 2020Quote: ... 5 μl of 2 mM biotin-alkyne-tag (Click Chemistry Tools, 1266), and 5 μl of 200 mM CuSO4 were added in the specified order to 5 ml of PBS (pH 7.8) ...
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bioRxiv - Biophysics 2023Quote: ... for further conjugation with DBCO-PEG4-NHS (Click Chemistry Tools, A134-2) via succinimidyl ester chemistry ...
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bioRxiv - Neuroscience 2024Quote: ... were submerged in dimethyl sulfoxide (DMSO) with 10 mM dibenzocyclooctyne-PEG-amine (DBCO-PEG4-amine; Click Chemistry Tools, A103P) overnight at room temperature ...
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bioRxiv - Microbiology 2022Quote: ... AF 488 picolyl azide and 5-ethynyl-2’-deoxycytidine (EdC) (Click Chemistry Tools, Scottsdale, AZ) were used for click chemistry according to the manufacturer’s instructions.
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bioRxiv - Microbiology 2023Quote: ... AlexaFlour 488 picolyl azide and 5-ethynyl-2’-deoxycytidine (EdC) (Click Chemistry Tools, Scottsdale, AZ) were used for click-chemistry according to the manufacturer’s instructions.
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bioRxiv - Synthetic Biology 2023Quote: ... and 44 µL of a 1:1 mixture of BTTAA (Click Chemistry Tools, 77.4 mg mL-1 PBS) and copper sulfate pentahydrate (7.4 mg mL-1 water) ...
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bioRxiv - Molecular Biology 2023Quote: ... Stocks of N-azidoacetylmannosamine-tetraacylated (Ac4ManNAz, Click Chemistry Tools) were made to 500 mM in sterile dimethyl sulfoxide (DMSO) ...
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bioRxiv - Microbiology 2023Quote: ... and then resuspended to OD600 of 0.6 in 1 mL PBS + 1 μM AZDye 488 DBCO (Click chemistry tools, cat# 1278-1). Cells were covered with aluminum foil and rocked for 1 hour ...
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bioRxiv - Molecular Biology 2021Quote: ... brucei cells (0.7-0.9 × 106 cells/ml) were incubated with 150 µM 5-ethynyl-2’-deoxyuridine (EdU) (Click Chemistry Tools) for 3 hrs before genomic DNA was isolated ...
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bioRxiv - Cell Biology 2023Quote: ... cells are incubated overnight in culture medium with 5 μM of 5-ethynyl 2’-deoxyuridine (EdU, #1149, Click Chemistry Tools) diluted from a 10 mM stock in DMSO ...
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bioRxiv - Bioengineering 2020Quote: ... cytokines were reacted with a commercial 2-nitrobenzyl linker displaying both NHS ester and DBCO substituents (1160-10, Click Chemistry Tools) followed by addition of poly(ethylene glycol ...
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bioRxiv - Biochemistry 2019Quote: ... artificial amino acid-containing base was incubated at room temperature with 150 µM 5,5′-dithiobis-2-nitrobenzoic acid for 10 minutes before chilling on ice and adding 300 µM DBCO-Cy3 (Click Chemistry Tools) and incubating at 4°C overnight ...
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bioRxiv - Plant Biology 2023Quote: ... 44.5 μL of labelled proteins were incubated with 2 μM Cy5 Picolyl Azide (Click Chemistry Tools, 50 μM stock in DMSO) or Fluorescein Picolyl Azide (Click Chemistry Tools ...
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bioRxiv - Plant Biology 2024Quote: ... 44.5 μl of labelled proteins were treated with 2 μM Cy5 Picolyl Azide (Click Chemistry Tools, 50 μM stock in DMSO) or Fluorescein Picolyl Azide (Click Chemistry Tools ...
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bioRxiv - Immunology 2020Quote: ... A 500 μL labeling reaction containing 0.5 mg of ovalbumin in 1X BBS and 50 μL of 2 mM mTz-PEG4-NHS in DMSO (0.2 mM final; Click Chemistry Tools, 1069), pH 8.5 was rotated at 4 °C overnight ...
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bioRxiv - Bioengineering 2023Quote: 7-(Diethylamino)-4- (hydroxymethyl)coumarin (DEAC-OH) was previously synthesized in-house (Method S3) and conjugated to 2-azidoacetic acid (Click Chemistry tools, 1081), 3-azidopropionic acid (Synthonix ...
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bioRxiv - Cell Biology 2024Quote: ... CalFluor 647 Azide(Click chemistry tools, 1272-1); Phosphatidylcholine Assay Kit (Colorimetric/Fluorometric ...
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bioRxiv - Biochemistry 2020Quote: ... Lysates containing 50 μg of protein were then incubated for 2 h with shaking at 37°C with 100 μM TAMRA azide (Click Chemistry Tools Scottsdale, Az), 800 μM copper(II ...
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bioRxiv - Bioengineering 2024Quote: ... and were used for conjugation with dibenzocyclooctyne (DBCO)-N-hydroxysuccinimidyl (NHS) ester (#A133, Click Chemistry Tools). Typically ...
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bioRxiv - Cancer Biology 2023Quote: ... Monoclonal antibodies at 1 mg mL-1 in 1X PBS were incubated with DBCO-PEG5-NHS Ester linker (A102P, Click Chemistry Tools, Scottsdale, AZ, USA) at a 4:1 molar ratio of crosslinker to antibody for at least 2 h at room temperature ...
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bioRxiv - Microbiology 2023Quote: ... and 24 μM carboxyrhodamine 110 Alkyne (Click Chemistry Tools, TA106-1). Cells were washed 3x in PBS before acquisition via FACS or ImageStream.