Labshake search
Citations for Click Chemistry Tools :
51 - 77 of 77 citations for 7 Methylimidazo 1 2 a pyridine 6 boronic acid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Neuroscience 2020Quote: ... with 25 μM Alkyne Alexa fluor 405 (Click Chemistry tools # 1309-1), CuSO4 and additive overnight at 4°C ...
-
bioRxiv - Biochemistry 2022Quote: ... and 100× molar equivalents of DBCO-AF647 (Click Chemistry Tools, 1302-1) in DMSO were added and the reaction was mixed at 500 rpm in the dark for 2 hours rt ...
-
bioRxiv - Bioengineering 2020Quote: ... incubated for 40 minutes in AFDye 488 DBCO (Click Chemistry Tools, 1278-1; 30 μM) at 37°C ...
-
bioRxiv - Biochemistry 2022Quote: ... MCF10A cells were treated with 1 mM 5-ethynyl uridine (5-EU; Click Chemistry Tools 1261) for 1 h to label nascent RNA as in (Bryant et al ...
-
bioRxiv - Bioengineering 2023Quote: ... they were reacted with 10:1 Methyltetrazine-sulfo-N-Hydroxysuccinimide ester (Met-NHS) (Click Chemistry Tools):Peptides for an hour at room temperature in Dulbecco’s Phosphate Buffered Saline (DPBS ...
-
bioRxiv - Biochemistry 2023Quote: ... εmax = 250,000 M-1cm-1 for Cy5 as provided in the product specifications by Click Chemistry Tools. MWFis1ΔN = 13,584 daltons and MWDrp1 = 81,877 daltons.
-
bioRxiv - Microbiology 2023Quote: ... Bacteria were pulse-labeled for 60 min by supplementing 1 mM L-AHA (Click Chemistry Tools, 1066-100) or L-methionine (Sigma ...
-
bioRxiv - Molecular Biology 2024Quote: ... The 3’-amino modified oligos (Supplementary Table S5) were activated by 1 mM NHS-methyltetrazine (Click Chemistry Tools) in 50% DMSO for 60 min at 21 °C ...
-
bioRxiv - Bioengineering 2019Quote: ... The suspension was centrifuged and resuspended in 1% Tween 80 containing PBS with methyltetrazine-PEG4-Maleimide (Click Chemistry Tools) of 200 μM final concentration ...
-
bioRxiv - Cell Biology 2023Quote: ... were dissolved in dry DMF (100 µL) in a plastic screw cap tube and 3-azidopropyl-1-amine (3.7 μL, 37.2 μmol, Click Chemistry Tools) followed by DIEA (13 μL ...
-
bioRxiv - Molecular Biology 2024Quote: ... 25 µl of methanol-preserved cells (∼30’000 cells in total) were mixed with 1 mM NHS-TCO (Click Chemistry Tools) and incubated in the dark at 21 °C for 5 minutes ...
-
bioRxiv - Bioengineering 2020Quote: ... The reduced C-terminal cysteine (1 eq.) was reacted with sulfo DBCO-maleimide crosslinker (4 eq.) (Click Chemistry Tools, AZ, USA) in PBS (pH 6.5 ...
-
bioRxiv - Microbiology 2021Quote: ... Cells were resuspended in 50 μl of CuAAC reaction mixture (128 μM TBTA, 1 mM CuSO4, 1.2 mM freshly prepared sodium ascorbate, and 20 μM AFDye 488 Azide Plus fluorophore [Click Chemistry Tools] in PBS + 0.1% Triton X-100 + 0.01% BSA ...
-
bioRxiv - Bioengineering 2021Quote: ... two HBSS washes were performed and hydrogels were then incubated in AFDye 488 DBCO (Click Chemistry Tools, 1278-1; 30 μM) for 40 minutes at 37°C ...
-
bioRxiv - Cell Biology 2022Quote: ... The beads were then washed with ice-cold D-PBS 4 times and incubated in D-PBS supplemented with 1 μM Cy5.5-azide (Click Chemistry Tools), 1 mM TCEP (adjusted to pH 7.0) ...
-
bioRxiv - Molecular Biology 2022Quote: Cells were washed 3X with PBS/1%FBS (pH 7.4) followed by treatment with 10 μM CalFluor 647 Azide (Click Chemistry Tools), premixed BTTAA-CuSO4 complex ([BTTAA]:[CuSO4]=2:1 ...
-
bioRxiv - Molecular Biology 2022Quote: ... Cells were washed 3X with PBS/1%FBS (pH 7.4) followed by treatment with 10 μM CalFluor 647 Azide (Click Chemistry Tools), premixed BTTAA-CuSO4 complex ([BTTAA]:[CuSO4]=2:1 ...
-
bioRxiv - Microbiology 2023Quote: ... Cells were resuspended in 50 μl of CuAAC reaction mixture (128 μM TBTA, 1 mM CuSO4, 1.2 mM freshly prepared sodium ascorbate, and 20 μM AFDye 488 Azide Plus fluorophore [Click Chemistry Tools] in PBS + 0.1% Triton X-100 + 0.01% BSA ...
-
bioRxiv - Bioengineering 2021Quote: ... 50 μL of 1 mg/mL antibodies were reacted with 1 μL of 10 mM UV-cleavable azido-NHS ester (30 equivalents to antibodies; Click Chemistry Tools) for 2 h ...
-
Bio-orthogonal Glycan Imaging of Culture Cells and Whole Animal C. elegans with Expansion MicroscopybioRxiv - Bioengineering 2024Quote: ... elegans eggs were hatched into L1 larvae for 24 h without food in M9 buffer containing 1 mM tetra-acylated azidoacetylgalactosamine (GalNAz, Click Chemistry Tools) or M9 buffer containing equal volume of the vehicle (DMSO) ...
-
bioRxiv - Cell Biology 2023Quote: ... were dissolved in dry DMF (200 µL) in a plastic screw cap tube and 3-azidopropyl-1-amine (11 μL, 110 μmol, Click Chemistry Tools) followed by DIEA (38 μL ...
-
bioRxiv - Microbiology 2019Quote: ... with an azide or alkyne Alexa Fluor 488/594 (AF 594 Alkyne, 1297-1 or AF 488 Picolyl Azide 1276-5, Click Chemistry Tools, USA) for 1 hour in the dark ...
-
bioRxiv - Microbiology 2021Quote: ... 15 mL portions of each sample were resuspended in 15 mL of incubation media amended with 1 mM homopropargylglycine (HPG; Click Chemistry Tools, USA) (HPG-positive microcosm) ...
-
bioRxiv - Bioengineering 2024Quote: ... dicysteines resuspended at a 40 mM concentration were prereacted with 100 µM of AlexaFluor 488 maleimide (1:400 dye:peptide) (Click Chemistry Tools; Scottsdale, AZ) for 15 minutes ...
-
bioRxiv - Bioengineering 2024Quote: ... whereas PEG-BCN was preincubated for 15 minutes with 50 µM of AlexaFluor 568 azide (1:200 dye:PEG) (Click Chemistry Tools; Scottsdale, AZ). DN hydrogels were then formed with these fluorescently tagged components ...
-
bioRxiv - Microbiology 2022Quote: ... L-AHA-containing proteins were labeled with Cy7 by strain-promoted azide-alkyne click-chemistry employing 1 μM dibenzylcyclooctyne-Cy7 (DBCO-Cy7) (Click Chemistry Tools, USA, Scottsdale). After methanol-chloroform extraction ...
-
bioRxiv - Immunology 2024Quote: ... SAv-drug conjugation reactions were next incubated for 1 hour with 100 μL azide agarose slurry with gentle mixing (50 μL packed resin; Click Chemistry Tools/Vector Laboratories), after which the resin was removed using Spin-X columns (Corning) ...